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Registro Completo |
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Biblioteca(s): |
Embrapa Agropecuária Oeste. |
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Data corrente: |
17/01/2019 |
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Data da última atualização: |
29/01/2019 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
DUBOC, E.; MOTTA, I. de S.; SANTIAGO, E. F.; NASCIMENTO, A. M.; MEIRA, R.; MARTINI, L. V. R. |
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Afiliação: |
ENY DUBOC, CPAO; IVO DE SA MOTTA, CPAO; E. F. SANTIAGO, DOCENTE - UNIVERSIDADE ESTADUAL DE MATO GROSSO DO SUL; A. M. NASCIMENTO, GRADUANDO EM CIÊNCIAS BIOLÓGICAS - UNIVERSIDADE ESTADUAL DE MATO GROSSO DO SUL, DOURADOS; R. MEIRA, GRADUANDO EM CIÊNCIAS BIOLÓGICAS - UNIVERSIDADE ESTADUAL DE MATO GROSSO DO SUL, DOURADOS; L. V. R. MARTINI, GRADUANDO EM CIÊNCIAS BIOLÓGICAS - UNIVERSIDADE ESTADUAL DE MATO GROSSO DO SUL, DOURADOS. |
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Título: |
Produção de mudas de Maclura tinctoria com resíduo de poda em substrato orgânico. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: SEMINÁRIO ESTADUAL DE EDUCAÇÃO NO CAMPO, 3.; SEMINÁRIO DE AGROECOLOGIA DE MATO GROSSO DO SUL, 7.; ENCONTRO DE PRODUTORES AGROECOLÓGICOS DE MATO GROSSO DO SUL, 6.; Seminário de sistemas agroflorestais em bases agroecológicas de MATO GROSSO DO SUL, 3., 2018, Campo Grande, MS. Sistemas agroflorestais agroalimentares, sociobiodiversidade, saúde, educação: desafios e perspectivas. Campo Grande, MS, 2018. AGROECOL 2018 |
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Idioma: |
Português |
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Notas: |
Publicada também no Cadernos de Agroecologia, v. 13, n. 2, 2018. |
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Palavras-Chave: |
Lodo de esgoto; Taiúva; Vermicomposto. |
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Thesagro: |
Meio Ambiente; Reciclagem; Resíduo Orgânico. |
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Thesaurus Nal: |
Organic compounds; Packing material; Recycling; Sewage sludge; Vermicomposting. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/190816/1/Agroecol-Eny-Maclura.pdf
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Marc: |
LEADER 01298nam a2200301 a 4500
001 2104388
005 2019-01-29
008 2018 bl uuuu u00u1 u #d
100 1 $aDUBOC, E.
245 $aProdução de mudas de Maclura tinctoria com resíduo de poda em substrato orgânico.$h[electronic resource]
260 $aIn: SEMINÁRIO ESTADUAL DE EDUCAÇÃO NO CAMPO, 3.; SEMINÁRIO DE AGROECOLOGIA DE MATO GROSSO DO SUL, 7.; ENCONTRO DE PRODUTORES AGROECOLÓGICOS DE MATO GROSSO DO SUL, 6.; Seminário de sistemas agroflorestais em bases agroecológicas de MATO GROSSO DO SUL, 3., 2018, Campo Grande, MS. Sistemas agroflorestais agroalimentares, sociobiodiversidade, saúde, educação: desafios e perspectivas. Campo Grande, MS, 2018. AGROECOL 2018$c2018
500 $aPublicada também no Cadernos de Agroecologia, v. 13, n. 2, 2018.
650 $aOrganic compounds
650 $aPacking material
650 $aRecycling
650 $aSewage sludge
650 $aVermicomposting
650 $aMeio Ambiente
650 $aReciclagem
650 $aResíduo Orgânico
653 $aLodo de esgoto
653 $aTaiúva
653 $aVermicomposto
700 1 $aMOTTA, I. de S.
700 1 $aSANTIAGO, E. F.
700 1 $aNASCIMENTO, A. M.
700 1 $aMEIRA, R.
700 1 $aMARTINI, L. V. R.
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Registro original: |
Embrapa Agropecuária Oeste (CPAO) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
18/08/2021 |
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Data da última atualização: |
19/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PEREZ, V. K. C.; CUSTODIO, D. A. C.; SILVA, E. M. M.; OLIVEIRA, J. de; GUIMARÃES, A. S.; BRITO, M. A. V. P.; SOUZA-FILHO, A. F.; HEINEMANN, M. B.; LAGE, A. P.; DORNELES, E. M. S. |
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Afiliação: |
VERÓNICA K. C. PÉREZ, Universidade Federal de Lavras, Lavras; DIRCÉIA A. C. CUSTÓDIO, Universidade Federal de Lavras; EDUARDA M. M. SILVA, Universidade Federal de Lavras; JULIA DE OLIVEIRA, Universidade Federal de Lavras; ALESSANDRO DE SA GUIMARAES, CNPGL; MARIA A. V. P. BRITO; ANTÔNIO F. SOUZA-FILHO, Universidade de São Paulo; MARCOS B. HEINEMANN, Universidade de São Paulo; ANDREY P. LAGE, Universidade Federal de Minas Gerais; ELAINE M. S. DORNELES, Universidade Federal de Lavras. |
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Título: |
Virulence factors and antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis in Brazil. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 51, p. 2111-2122, 2020. |
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DOI: |
https://doi.org/10.1007/s42770-020-00363-5 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to evaluate virulence factors and genetic markers of antimicrobial resistance in 400 Staphylococcus aureus strains isolated from bovine mastitis in four Brazilian states, as well as to assess the association between these characteristics and field information. Virulence factors and drug resistance genes were identified by PCR screening. Biofilm-forming and hemolytic phenotype were detected using Congo red Tryptic Soy Broth and defibrinated sheep blood agar, respectively. Of all isolates, 83.5% were biofilm-forming and 98.5% strains exhibited biofilm gene icaAD, and a significant association between phenotype and genotype for biofilm was observed (P = 0.0005). Hemolysin genes were observed in 82.85% (hla+hlb+), 16.5% (hla+) and 0.75% (hlb+) isolates, whereas the hemolytic phenotype exhibited was complete and incomplete hemolysis in 64.25%, complete in 28.25%, incomplete in 4.75%, and negative in 2.75% of the strains. Virulence factors genes luk, seb, sec, sed, and tst were observed in 3.5%, 0.5%, 1%, 0.25%, and 0.74% isolates, respectively. The gene blaZ was detected in 82.03% of penicillin-resistant isolates, whereas tetK and aac(6′)-Ie?aph(2′)-Ia were observed in 33.87% and 45.15% of the tetracycline and aminoglycosides-resistant isolates, respectively. Fluoroquinolone resistance gene mepA was detected for the first time in S. aureus from bovine mastitis. Resistance genes tetM (3.22%), tetL (1.61%), ermA (14.29%), ermB (14.29%), ermC (33.3%), ermT (9.52%), ermY (4.76%), msrA (9.52%), and mphC (9.52%) were also detected among resistant isolates. No association between virulence factors or antimicrobialresistant genes and year of isolation, geographic origin, or antimicrobial resistance profile was observed. Our results showed that S. aureus strains isolated from bovine mastitis in the four Brazilian states sampled are mainly biofilmforming and hemolytic, whereas virulence genes associated with enterotoxins, luk and tst, were less frequently observed. Moreover, a wide variety of resistance genes that confer resistance to almost all classes of antimicrobial agents approved for use in animals and humans were found. Overall, the data point to a great pathogenic potential of S. aureus associated with bovine mastitis and to the non-negligible risks to public health of staphylococcal infections from animal origin. MenosThis study aimed to evaluate virulence factors and genetic markers of antimicrobial resistance in 400 Staphylococcus aureus strains isolated from bovine mastitis in four Brazilian states, as well as to assess the association between these characteristics and field information. Virulence factors and drug resistance genes were identified by PCR screening. Biofilm-forming and hemolytic phenotype were detected using Congo red Tryptic Soy Broth and defibrinated sheep blood agar, respectively. Of all isolates, 83.5% were biofilm-forming and 98.5% strains exhibited biofilm gene icaAD, and a significant association between phenotype and genotype for biofilm was observed (P = 0.0005). Hemolysin genes were observed in 82.85% (hla+hlb+), 16.5% (hla+) and 0.75% (hlb+) isolates, whereas the hemolytic phenotype exhibited was complete and incomplete hemolysis in 64.25%, complete in 28.25%, incomplete in 4.75%, and negative in 2.75% of the strains. Virulence factors genes luk, seb, sec, sed, and tst were observed in 3.5%, 0.5%, 1%, 0.25%, and 0.74% isolates, respectively. The gene blaZ was detected in 82.03% of penicillin-resistant isolates, whereas tetK and aac(6′)-Ie?aph(2′)-Ia were observed in 33.87% and 45.15% of the tetracycline and aminoglycosides-resistant isolates, respectively. Fluoroquinolone resistance gene mepA was detected for the first time in S. aureus from bovine mastitis. Resistance genes tetM (3.22%), tetL (1.61%), ermA (14.29%), ermB (14.29%), ermC (33.3%), er... Mostrar Tudo |
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Palavras-Chave: |
Hemolisina; Infecção intramamária; Intramammary infection; Mastite; Staphylococci. |
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Thesagro: |
Biofilme; Bovino; Doença Animal; Estafilococo. |
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Thesaurus NAL: |
Biofilm; Hemolysins. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03482naa a2200373 a 4500
001 2133706
005 2021-08-19
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s42770-020-00363-5$2DOI
100 1 $aPEREZ, V. K. C.
245 $aVirulence factors and antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis in Brazil.$h[electronic resource]
260 $c2020
520 $aThis study aimed to evaluate virulence factors and genetic markers of antimicrobial resistance in 400 Staphylococcus aureus strains isolated from bovine mastitis in four Brazilian states, as well as to assess the association between these characteristics and field information. Virulence factors and drug resistance genes were identified by PCR screening. Biofilm-forming and hemolytic phenotype were detected using Congo red Tryptic Soy Broth and defibrinated sheep blood agar, respectively. Of all isolates, 83.5% were biofilm-forming and 98.5% strains exhibited biofilm gene icaAD, and a significant association between phenotype and genotype for biofilm was observed (P = 0.0005). Hemolysin genes were observed in 82.85% (hla+hlb+), 16.5% (hla+) and 0.75% (hlb+) isolates, whereas the hemolytic phenotype exhibited was complete and incomplete hemolysis in 64.25%, complete in 28.25%, incomplete in 4.75%, and negative in 2.75% of the strains. Virulence factors genes luk, seb, sec, sed, and tst were observed in 3.5%, 0.5%, 1%, 0.25%, and 0.74% isolates, respectively. The gene blaZ was detected in 82.03% of penicillin-resistant isolates, whereas tetK and aac(6′)-Ie?aph(2′)-Ia were observed in 33.87% and 45.15% of the tetracycline and aminoglycosides-resistant isolates, respectively. Fluoroquinolone resistance gene mepA was detected for the first time in S. aureus from bovine mastitis. Resistance genes tetM (3.22%), tetL (1.61%), ermA (14.29%), ermB (14.29%), ermC (33.3%), ermT (9.52%), ermY (4.76%), msrA (9.52%), and mphC (9.52%) were also detected among resistant isolates. No association between virulence factors or antimicrobialresistant genes and year of isolation, geographic origin, or antimicrobial resistance profile was observed. Our results showed that S. aureus strains isolated from bovine mastitis in the four Brazilian states sampled are mainly biofilmforming and hemolytic, whereas virulence genes associated with enterotoxins, luk and tst, were less frequently observed. Moreover, a wide variety of resistance genes that confer resistance to almost all classes of antimicrobial agents approved for use in animals and humans were found. Overall, the data point to a great pathogenic potential of S. aureus associated with bovine mastitis and to the non-negligible risks to public health of staphylococcal infections from animal origin.
650 $aBiofilm
650 $aHemolysins
650 $aBiofilme
650 $aBovino
650 $aDoença Animal
650 $aEstafilococo
653 $aHemolisina
653 $aInfecção intramamária
653 $aIntramammary infection
653 $aMastite
653 $aStaphylococci
700 1 $aCUSTODIO, D. A. C.
700 1 $aSILVA, E. M. M.
700 1 $aOLIVEIRA, J. de
700 1 $aGUIMARÃES, A. S.
700 1 $aBRITO, M. A. V. P.
700 1 $aSOUZA-FILHO, A. F.
700 1 $aHEINEMANN, M. B.
700 1 $aLAGE, A. P.
700 1 $aDORNELES, E. M. S.
773 $tBrazilian Journal of Microbiology$gv. 51, p. 2111-2122, 2020.
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