Data/hora: 10/06/2024 10:48:53

Biblioteca(s): | Embrapa Amazônia Ocidental; Embrapa Pesca e Aquicultura.
Data corrente: | 03/11/2021
Data da última atualização: | 04/11/2021
Autoria: | ADOLFI, M. C.; DU, K.; KNEITZ, S.; CABAU, C.; ZAHM, M.; KLOPP, C.; FERON, R.; PAIXÃO, R. V.; VARELA, E. S.; O'SULLIVAN, F. L. A.; OLIVEIRA, M. A. de; NÓBREGA, R. H.; LOPEZ-ROQUES, C.; IAMPIETRO, C.; LLUCH, J.; KLOAS, W.; WUERTZ, S.; SCHAEFER, F.; STÖCK, M.; GUIGUEN, Y.; SCHARTL, M.
Afiliação: | MATEUS C. ADOLFI, University of Wuerzburg; KANG DU, University of Wuerzburg; SUSANNE KNEITZ, University of Wuerzburg; CÉDRIC CABAU, Université de Toulouse; MARGOT ZAHM, Université de Toulouse; CHRISTOPHE KLOPP, INRA; ROMAIN FERON, INRAE; RÔMULO V. PAIXÃO, Bolsista CPAA; EDUARDO SOUSA VARELA, CNPASA; FERNANDA LOUREIRO ALMEIDA OSULLIVAN, CPAA; MARCOS A. DE OLIVEIRA, UNESP; RAFAEL H. NÓBREGA, UNESP; CÉLINE LOPEZ-ROQUES, INRAE; CAROLE IAMPIETRO, INRAE; JÉRÔME LLUCH, INRAE; WERNER KLOAS, Institute of Freshwater Ecology and Inland Fisheries; SVEN WUERTZ, Institute of Freshwater Ecology and Inland Fisheries; FABIAN SCHAEFER, Institute of Freshwater Ecology and Inland Fisheries; MATTHIAS STÖCK, Institute of Freshwater Ecology and Inland Fisheries; YANN GUIGUEN, INRAE; MANFRED SCHARTL, Comprehensive Cancer Center Mainfranken.
Título: | A duplicated copy of id2b is an unusual sex-determining candidate gene on the Y chromosome of arapaima (Arapaima gigas).
Ano de publicação: | 2021
Fonte/Imprenta: | Scientific Reports, v. 11, art. 21544, 2021.
ISSN: | 2045-2322
DOI: | https://doi.org/10.1038/s41598-021-01066-z
Idioma: | Inglês
Conteúdo: | Arapaima gigas is one of the largest freshwater fish species of high ecological and economic importance. Overfishing and habitat destruction are severe threats to the remaining wild populations. By incorporating a chromosomal Hi-C contact map, we improved the arapaima genome assembly to chromosome-level, revealing an unexpected high degree of chromosome rearrangements during evolution of the bonytongues (Osteoglossiformes). Combining this new assembly with pool-sequencing of male and female genomes, we identified id2bbY, a duplicated copy of the inhibitor of DNA binding 2b (id2b) gene on the Y chromosome as candidate male sex-determining gene. A PCR-test for id2bbY was developed, demonstrating that this gene is a reliable male-specific marker for genotyping. Expression analyses showed that this gene is expressed in juvenile male gonads. Its paralog, id2ba, exhibits a male-biased expression in immature gonads. Transcriptome analyses and protein structure predictions confirm id2bbY as a prime candidate for the master sex-determiner. Acting through the TGF B signaling pathway, id2bbY from arapaima would provide the first evidence for a link of this family of transcriptional regulators to sex determination. Our study broadens our current understanding about the evolution of sex determination genetic networks and provide a tool for improving arapaima aquaculture for commercial and conservation purposes.
Thesagro: | Cromossoma; Peixe; Pirarucu.
Thesaurus Nal: | Arapaima gigas; Chromosomes; Cytogenetics; Fish; Sex chromosomes; Sex determination.

Categoria do assunto:

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L Ciência Animal e Produtos de Origem Animal

URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/227368/1/Arapaima-sexmaster-gene-2021.pdf
Registro original: | Embrapa Pesca e Aquicultura (CNPASA)

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