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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
05/01/2001 |
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Data da última atualização: |
05/01/2001 |
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Autoria: |
RITCHIE, N. J.; SCHUTTER, M. E.; DICK, R. P.; MYROLD, D. D. |
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Título: |
Use of length heterogeneity PCR and fatty acid methyl ester profiles to characterize microbial communities in soil. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Applied and Environmental Microbiology, Washington, v. 66, p. 1668-1675, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
In length heterogeneity PCR (LH-PCR) a fluorescently labeled primer is used to determine the relative amounts of amplified originating from different microorganisms. Labeled fragments are separated by gel electrophoresis and detected by laser-induced fluorescence with an automated gene sequencer. We used LH-PCR to evaluate the composition of the soil microbial community. Four soils, which differed in terms of soil type and/or crop management practice, were studied. Previous data for microbial biomass, nitrogen and carbon contents, and nitrogen mineralization rates suggested that the microbial characteristics of these soils were different. One site received two different treatments: no-till and conventional till perennial ryegrass. The other sites were no-till continuous grass plots at separate locations with different soil types. Community composition was characterized by assessing the natural length heterogeneity in eubacterial sequences amplified from the 5' domain of the 16S rRNA gene and by determining fatty acid methyl ester (FAME) profiles. We found that LH-PCR results were reproducible. Both methods distinguished the three sites. The most abundant bacterial community members, based on cloned LH-PCR products, were members of the B subclass of the class Proteobacteria, the Cytophaga-Flexibacter-Bacteriodes group, and the high-G+C-content gram-positive bacterial group. Strong correlations were found between LH-PCR results and FAME results. We found that the LH-PCR method is an efficient, reliable, and highly reproducible method that should be a useful tool in future assessments of microbial community composition. MenosIn length heterogeneity PCR (LH-PCR) a fluorescently labeled primer is used to determine the relative amounts of amplified originating from different microorganisms. Labeled fragments are separated by gel electrophoresis and detected by laser-induced fluorescence with an automated gene sequencer. We used LH-PCR to evaluate the composition of the soil microbial community. Four soils, which differed in terms of soil type and/or crop management practice, were studied. Previous data for microbial biomass, nitrogen and carbon contents, and nitrogen mineralization rates suggested that the microbial characteristics of these soils were different. One site received two different treatments: no-till and conventional till perennial ryegrass. The other sites were no-till continuous grass plots at separate locations with different soil types. Community composition was characterized by assessing the natural length heterogeneity in eubacterial sequences amplified from the 5' domain of the 16S rRNA gene and by determining fatty acid methyl ester (FAME) profiles. We found that LH-PCR results were reproducible. Both methods distinguished the three sites. The most abundant bacterial community members, based on cloned LH-PCR products, were members of the B subclass of the class Proteobacteria, the Cytophaga-Flexibacter-Bacteriodes group, and the high-G+C-content gram-positive bacterial group. Strong correlations were found between LH-PCR results and FAME results. We found that the LH-PCR method... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02152naa a2200157 a 4500
001 1621188
005 2001-01-05
008 2000 bl --- 0-- u #d
100 1 $aRITCHIE, N. J.
245 $aUse of length heterogeneity PCR and fatty acid methyl ester profiles to characterize microbial communities in soil.
260 $c2000
520 $aIn length heterogeneity PCR (LH-PCR) a fluorescently labeled primer is used to determine the relative amounts of amplified originating from different microorganisms. Labeled fragments are separated by gel electrophoresis and detected by laser-induced fluorescence with an automated gene sequencer. We used LH-PCR to evaluate the composition of the soil microbial community. Four soils, which differed in terms of soil type and/or crop management practice, were studied. Previous data for microbial biomass, nitrogen and carbon contents, and nitrogen mineralization rates suggested that the microbial characteristics of these soils were different. One site received two different treatments: no-till and conventional till perennial ryegrass. The other sites were no-till continuous grass plots at separate locations with different soil types. Community composition was characterized by assessing the natural length heterogeneity in eubacterial sequences amplified from the 5' domain of the 16S rRNA gene and by determining fatty acid methyl ester (FAME) profiles. We found that LH-PCR results were reproducible. Both methods distinguished the three sites. The most abundant bacterial community members, based on cloned LH-PCR products, were members of the B subclass of the class Proteobacteria, the Cytophaga-Flexibacter-Bacteriodes group, and the high-G+C-content gram-positive bacterial group. Strong correlations were found between LH-PCR results and FAME results. We found that the LH-PCR method is an efficient, reliable, and highly reproducible method that should be a useful tool in future assessments of microbial community composition.
700 1 $aSCHUTTER, M. E.
700 1 $aDICK, R. P.
700 1 $aMYROLD, D. D.
773 $tApplied and Environmental Microbiology, Washington$gv. 66, p. 1668-1675, 2000.
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Embrapa Agrobiologia (CNPAB) |
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| 13. | | PACHECO, S.; GODOY, R. L. O.; PORTE, A.; ROSA, J. S. da; ARAÚJO, M. C. P. Obtaining zeaxanthin and violaxanthin standards for high performance liquid chromatography from solanum pseudocapsicum and capsicum annuum. In: WORLD CONGRESS OF FOOD SCIENCE AND TECHNOLOGY, 16.; LATIN AMERICAN SEMINAR OF FOOD SCIENCE AND TECHNOLOGY, 17., 2012, Foz do Iguaçu. Addressing global food security and wellness through food science and technology: abstracts. Foz do Iguaçu: IUFoST: ALACCTA, 2012. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
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| 18. | | ARAUJO, M. C.; DONATONI, F. A. B.; PEZZOPANE, C. de G.; PROENÇA, T. M.; SANTOS, P. M. Atividade enzimática da guaiacol peroxidase de Urochloa brizantha cv. BRS Paiaguás em condições de estresse hídrico. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 9., 2017, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste; Embrapa Instrumentação, 2017. p. 54. (Embrapa Pecuária Sudeste. Documentos, 126). Editores técnicos: Alexandre Berndt, Ana Rita Araujo Nogueira, Bianca Baccili Zanotto Vigna, Juliana Gonçalves Costa, Lea Chapaval, Manuel Antonio Chagas Jacinto, Patricia Menezes Santos.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 19. | | BRITO, E. S. de; ARAÚJO, M. C. P. de; ALVES, R. E.; CARKEET, C.; CLEVIDENCE, B. A.; NOVOTNY, J. A. Anthocyanins present in selected tropical fruits: acerola, jambolão, and guajiru. Journal of Agricultural and Food Chemistry, Washington, DC, v.55, n.23. p.9389-9394, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
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| 20. | | BRITO, E. S. de; ARAÚJO, M. C. P. de; ALVES, R. E.; CARKEET, C.; CLEVIDENCE, B. A.; NOVOTNY, J. A. Anthocyanins present in selected tropical fruits: acerola, jambolão, and guajiru. Journal of Agricultural and Food Chemistry, Washington, DC, v. 55, n. 23, p. 9389-9394, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
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| Registros recuperados : 80 | |
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