Data/hora: 06/06/2024 16:23:19

Biblioteca(s): | Embrapa Clima Temperado; Embrapa Recursos Genéticos e Biotecnologia.
Data corrente: | 07/01/2019
Data da última atualização: | 18/12/2019
Autoria: | MATTOS, V. da S.; MULET, K.; CARES, J. E.; GOMES, C. B.; FERNANDEZ, D.; SA, M. F. G. de; CARNEIRO, R. M. D. G.; CASTAGNONE-SERENO, P.
Afiliação: | VANESSA DA SILVA MATTOS, UNB; KARINE MULET, CNRS; JUVENIL ENRIQUE CARES, UNB; CESAR BAUER GOMES, CPACT; DIANA FERNANDEZ, IRD; MARIA FATIMA GROSSI DE SA, Cenargen; REGINA MARIA DECHECHI G CARNEIRO, Cenargen; PHILIPPE CASTAGNONE-SERENO, INRA.
Título: | Development of Diagnostic SCAR Markers for Meloidogyne graminicola, M. Oryzae, and M. salasi associated with irrigated rice fields in Americas.
Ano de publicação: | 2019
Fonte/Imprenta: | Plant Disease, v. 103, p. 83-88, Jan. 2019.
DOI: | https://doi.org/10.1094/PDIS-12-17-2015-RE

Idioma:

Inglês
Português

Conteúdo: | Root-knot nematodes (RKN) cause important production losses of rice (Oryza sativa L.) in the world. Together with Meloidogyne graminicola Golden and Birchfield 1965, M. oryzae Maas, Sanders and Dede, 1978 and M. salasi López, 1984 have been causing damages in irrigated rice fields in Central and South America. In addition, six other RKN species may occur in rice fields in other regions of the world. Correct identification of Meloidogyne spp. is difficult but essential for the management of rice RKNs. The objective of this study was to develop some species-specific molecular markers for the diagnosis of South American RKN rice-related species. Isozyme phenotypes indicated the occurrence of some RKN species in the Brazilian samples, namely M. graminicola, M. oryzae, M. javanica, and two cryptic species designated as Meloidogyne sp. 2 and Meloidogyne sp. 3. Random amplified polymorphic DNA (RAPD) analysis of 16 isolates revealed interspecific genetic polymorphism between Meloidogyne spp., but isolates belonging to the same species (i.e., sharing the same esterase phenotype) always clustered together, whatever the species considered. Specific SCAR markers of 230, 120, and 160 bp were developed for M. graminicola, M. oryzae, and M. salasi, respectively. These SCAR markers may be potential molecular tools for application in routine diagnostic procedures subject to their validation with other rice RKN field populations in the world.
Palavras-Chave: | Nematóide das galhas; South American; Species-specific molecular markers.
Thesagro: | Marcador Molecular; Nematóide; Oryza Sativa.
Thesaurus Nal: | Root-knot nematodes.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/189842/1/Cesar-Bauer-pdis-12-17-2015-re.pdf
Registro original: | Embrapa Clima Temperado (CPACT)

Exemplares:

CPACT - (21047-1) - UPC - AP - DD -  -  -  - DIS
CENARGEN - (37596-1) - UPC - AP - DD -  -  -  - DIS