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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
02/05/2014 |
Data da última atualização: |
02/05/2014 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MOURA, R. R.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; MELO, C. H. S.; SANCHEZ, D. J. D.; VIEIRA, M. P.; ALMEIDA, T. M.; SEROVA, I. A.; SEROV, O. L.; PEREIRA, A. F.; TEIXEIRA, D. I. A.; MELO, L. M.; FREITAS, V. J. F. |
Afiliação: |
Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; JEFERSON FERREIRA DA FONSECA, CNPC; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; Institute of Cytology and Genetics, Novosibirsk, Russia.; Institute of Cytology and Genetics, Novosibirsk, Russia.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil.; UECE - Fortaleza, CE, Brazil. |
Título: |
Reproductive parameters and the use of MOET in transgenic founder goat carrying the human granulocyte colony-stimulating factor (hG-CSF) gene. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Animal Reproduction, v. 11, n. 1, p. 37-43, Jan./Mar. 2014. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: This study aimed to monitor estrous cycle parameters of a human granulocyte colony-stimulating factor (hG-CSF)-transgenic founder female goat and to perform superovulation and embryo recovery (surgical or transcervical method) for further transfer to recipients to quickly obtain offspring. Two experiments were performed using a transgenic (TF) and a non-transgenic (NTF) female. In experiment 1, three estrous cycles were monitored for the following parameters: estrus behavior, progesterone concentration and ovarian activity. In experiment 2, two superovulation/embryo recovery sessions were performed and the recovered embryos were transferred to previously prepared recipients. Data were compared by either t test or Fisher's exact test. The mean interval between natural estrus was 20.7 ± 0.6 and 19.7 ± 0.6 (P > 0.05) days for the TF and NTF, respectively. Progesterone concentrations and ovarian activity were normal and similar between goats. The ovulation rate was similar between TF and NTF (12.0 ± 1.4 vs. 18.0 ± 4.2 CL; P > 0.05). No significant differences in embryo recovery rate (P > 0.05) were observed between the surgical and transcervical methods for TF (69.2 vs. 72.7%) or NTF (100.0 vs. 86.7%). Sixteen embryos from the TF were transferred to recipients, and eight kids were born. Among these kids, the transgene was identified in three (two males and one female), resulting in a transgenesis rate of 37.5%. In summary, the TF is a true founder, since she proved fertility and capacity of transmitting the hG-CSF transgene to progeny, suggesting that the analyzed reproductive traits were not compromised by the presence of the transgene. MenosAbstract: This study aimed to monitor estrous cycle parameters of a human granulocyte colony-stimulating factor (hG-CSF)-transgenic founder female goat and to perform superovulation and embryo recovery (surgical or transcervical method) for further transfer to recipients to quickly obtain offspring. Two experiments were performed using a transgenic (TF) and a non-transgenic (NTF) female. In experiment 1, three estrous cycles were monitored for the following parameters: estrus behavior, progesterone concentration and ovarian activity. In experiment 2, two superovulation/embryo recovery sessions were performed and the recovered embryos were transferred to previously prepared recipients. Data were compared by either t test or Fisher's exact test. The mean interval between natural estrus was 20.7 ± 0.6 and 19.7 ± 0.6 (P > 0.05) days for the TF and NTF, respectively. Progesterone concentrations and ovarian activity were normal and similar between goats. The ovulation rate was similar between TF and NTF (12.0 ± 1.4 vs. 18.0 ± 4.2 CL; P > 0.05). No significant differences in embryo recovery rate (P > 0.05) were observed between the surgical and transcervical methods for TF (69.2 vs. 72.7%) or NTF (100.0 vs. 86.7%). Sixteen embryos from the TF were transferred to recipients, and eight kids were born. Among these kids, the transgene was identified in three (two males and one female), resulting in a transgenesis rate of 37.5%. In summary, the TF is a true founder, since she proved fer... Mostrar Tudo |
Palavras-Chave: |
Goat; Transgenia. |
Thesagro: |
Cabra; Caprino; Hormônio animal; Progesterona; Superovulação; Transferência de embrião. |
Thesaurus Nal: |
Embryo transfer; Progesterone; Superovulation; Transgenesis. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/101750/1/API-Reproductive-parameters.pdf
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Marc: |
LEADER 02866naa a2200409 a 4500 001 1985516 005 2014-05-02 008 2014 bl uuuu u00u1 u #d 100 1 $aMOURA, R. R. 245 $aReproductive parameters and the use of MOET in transgenic founder goat carrying the human granulocyte colony-stimulating factor (hG-CSF) gene.$h[electronic resource] 260 $c2014 520 $aAbstract: This study aimed to monitor estrous cycle parameters of a human granulocyte colony-stimulating factor (hG-CSF)-transgenic founder female goat and to perform superovulation and embryo recovery (surgical or transcervical method) for further transfer to recipients to quickly obtain offspring. Two experiments were performed using a transgenic (TF) and a non-transgenic (NTF) female. In experiment 1, three estrous cycles were monitored for the following parameters: estrus behavior, progesterone concentration and ovarian activity. In experiment 2, two superovulation/embryo recovery sessions were performed and the recovered embryos were transferred to previously prepared recipients. Data were compared by either t test or Fisher's exact test. The mean interval between natural estrus was 20.7 ± 0.6 and 19.7 ± 0.6 (P > 0.05) days for the TF and NTF, respectively. Progesterone concentrations and ovarian activity were normal and similar between goats. The ovulation rate was similar between TF and NTF (12.0 ± 1.4 vs. 18.0 ± 4.2 CL; P > 0.05). No significant differences in embryo recovery rate (P > 0.05) were observed between the surgical and transcervical methods for TF (69.2 vs. 72.7%) or NTF (100.0 vs. 86.7%). Sixteen embryos from the TF were transferred to recipients, and eight kids were born. Among these kids, the transgene was identified in three (two males and one female), resulting in a transgenesis rate of 37.5%. In summary, the TF is a true founder, since she proved fertility and capacity of transmitting the hG-CSF transgene to progeny, suggesting that the analyzed reproductive traits were not compromised by the presence of the transgene. 650 $aEmbryo transfer 650 $aProgesterone 650 $aSuperovulation 650 $aTransgenesis 650 $aCabra 650 $aCaprino 650 $aHormônio animal 650 $aProgesterona 650 $aSuperovulação 650 $aTransferência de embrião 653 $aGoat 653 $aTransgenia 700 1 $aSOUZA-FABJAN, J. M. G. 700 1 $aFONSECA, J. F. da 700 1 $aMELO, C. H. S. 700 1 $aSANCHEZ, D. J. D. 700 1 $aVIEIRA, M. P. 700 1 $aALMEIDA, T. M. 700 1 $aSEROVA, I. A. 700 1 $aSEROV, O. L. 700 1 $aPEREIRA, A. F. 700 1 $aTEIXEIRA, D. I. A. 700 1 $aMELO, L. M. 700 1 $aFREITAS, V. J. F. 773 $tAnimal Reproduction$gv. 11, n. 1, p. 37-43, Jan./Mar. 2014.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registros recuperados : 5 | |
1. | | MOURA, R. R.; MONTEIRO, D. A.; FONSECA, E. da S.; BALIEIRO, F. de C.; CESÁRIO, F.; RACHID, C. T. C. da C. The impact of pH modulation on the rhizosphere bacteriome of maize and bean. Microbiology Research, v. 15, n. 1, p. 314-325, 2024.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
Biblioteca(s): Embrapa Solos. |
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2. | | MOURA, N. O.; SAKREZENSKI, D. A.; PAIXÃO, D. S.; MARTINS, C. L.; SILVA, E. B. M.; EL-HUSNY, J. C.; SILVA, A. G.; CARDOSO, K. W. S.; MOURA, R. R. O.; FREITAS, L. S. Infestação de lagartas falsa-medideira Chrysodeixis includens (Lepidoptera: Noctuidae) em cultivares de soja no polo Paragominas de grãos, Pará. In: CONGRESSO BRASILEIRO DE SOJA, 8., 2018, Goiânia. Inovação, tecnologias digitais e sustentabilidade da soja: anais. Brasília, DF: Embrapa, 2018. p. 113-115.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Amazônia Oriental. |
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3. | | MOURA, R. R.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; MELO, C. H. S.; SANCHEZ, D. J. D.; VIEIRA, M. P.; ALMEIDA, T. M.; SEROVA, I. A.; SEROV, O. L.; PEREIRA, A. F.; TEIXEIRA, D. I. A.; MELO, L. M.; FREITAS, V. J. F. Reproductive parameters and the use of MOET in transgenic founder goat carrying the human granulocyte colony-stimulating factor (hG-CSF) gene. Animal Reproduction, v. 11, n. 1, p. 37-43, Jan./Mar. 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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4. | | MOURA, R. R.; SOUZA, J. M. G. de; ALMEIDA, T. M. de; VIEIRA, M. P.; MELO, C. H. S. de; BATISTA, R. I. T. P.; PEREIRA, A. F.; TEIXEIRA, D. I. A.; MELO, L. M.; FONSECA, J. F. da; FREITAS, V. J. de F. Uso da colheita transcervical de embriões em cabra transgênica para o fator estimulante de colônia de granulócitos humano (HG-CSF). In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 25., 2011, Cumbuco. O impacto das biotecnologias reprodutivas na saúde e produção animal: anais. Cumbuco: SBTE, 2011. p. 422. Resumo R224. 1 CD-ROM.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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5. | | MOURA, R. R.; SOUZA, J. M. G.; MELO, C. H. S.; ALBUQUERQUE, E. S.; ALMEIDA, T. M.; VIEIRA, M. P.; BATISTA, R. I. T. P.; PEREIRA, A. F.; TEIXEIRA, D. I. A.; MELO, L. M.; FONSECA, J. F. da; FREITAS, V. J. F. Use of two methods for embryo recovery in an embryo transfer program from a founder transgenic goat for human granulocyte colony-Stimulating Factor (hG-CSF). Transgenic Research, v. 21, n. 4, p. 921, Aug. 2012. Abstracts from the UC Davis Transgenic Animal Research Conference VIII, August, 2011.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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Registros recuperados : 5 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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