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| Registros recuperados : 14 | |
| 2. |  | CANTUARIAS-AVILÉS, T.; STUCHI, E. S.; MOURÃO FILHO, F. A. A.; SILVA, S. R. Horticultural performance of Tahiti lime onto twelve rootstocks in Northern São Paulo State, Brazil. In: INTERNATIONAL HORTICULTURAL CONGRESS, 28., 2010, Lisboa. Science and horticulture for people: programme & book of abstracts. Lisboa: ISHS, 2010. v. 1, p. 149. 1 CD-ROM. T02.227, pdf.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 5. | | CANTUARIAS-AVELÉS, T.; STUCHI, E. S.; MOURÃO FILHO, F. A. A.; RODRIGUES-SILVA, S. Effects of trifoliate rootstocks on growth, productivity and fruit quality of 'Tahiti' lime in Brazil (Citrus latifolia Hort. ex. Tanaka). In: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan, China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 133. P41.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 6. | | CANTUARIAS-AVILÉS, T.; STUCHI, E. S.; MOURÃO FILHO, F. A. A.; SILVA, S. R. Twelve rootstocks effects on the intensity of citrus variagated chlorosis (CVC) in 'Folha Murcha' sweet orange in Bebedouro, SP, Brazil. In: CONFERENCE OF THE INTERNATIONAL ORGANIZATION OF CITRUS VIROLOGISTS, 17., 2007, Adana. Program [and] abstracts... Adana: IOCV, 2007. p. 88.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 9. | | MOURÃO FILHO, F. A. A.; STUCHI, E. S.; MENDES, B. M. J.; CANTUARIAS-AVILÉS, T.; SILVA, S. R. Plant growth and fruit yield of Valencia sweet orange grafted on thirteen rootstocks. In: INTERNATIONAL HORTICULTURAL CONGRESS, 28., 2010, Lisboa. Science and horticulture for people: programme & book of abstracts. Lisboa: ISHS, 2010. v. 1, p. 147. 1 CD-ROM. T02.219, pdf.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 12. | | MOURÃO FILHO, F. A. A.; STUCHI, E. S.; CANTUARIAS-AVILÉS, T.; ESPINOZA-NÚÑEZ, E.; BREMER NETO, H.; BASSAN, M. M.; SILVA, S. R. Horticultural package for high quality Tahiti lime production. In: INTERNATIONAL CITRUS CONGRESS, 22., 2012.Valencia. Book of abstracts. Valencia: International Society of Citriculture; Instituto Valenciano de Investigaciones Agrarias; Fundación Agroalimed, 2012. Documento eletrônico.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 13. | | RAMOS, Y. C.; MOURÃO FILHO, F. A. A.; STUCHI, E. S.; SILVA, S. R; CAPUTO, M. M.; BREMER NETO, H.; FADEL, A. L. Initial production of 17 mid-season sweet orange cultivars in Southwestern São Paulo State, Brazil. In: INTERNATIONAL CITRUS CONGRESS, 22., 2012.Valencia. Book of abstracts. Valencia: International Society of Citriculture; Instituto Valenciano de Investigaciones Agrarias; Fundación Agroalimed, 2012. Documento eletrônico.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 14. | | SALIBE, A. B.; MOURÃO FILHO, F. A. A.; LARANJEIRA, F. F.; MACHADO, M.; BRAGA, G. C.; GAZOLA, D.; REOLON, C. A. Eficiência de métodos de transmissão de Xylella fastidiosa em plantas citricas. Fitopatologia Brasileira, Brasília, DF, v. 32, p. 121, ago. 2007. Suplemento. Edição dos Resumos do XL Congresso Brasiliero de Fitopatologia, Maringá, ago. 2007.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 14 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
14/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; REIS, D. R. de L.; FERNANDES, L. E.; PEIXOTO, M. G. C. D.; MACHADO, M. A.; VIANA, J. H. M. |
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Afiliação: |
S. Wohlres-Viana, UFJF; E. K. N. Arashiro, UFJF; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; L. E. Fernandes; MARIA GABRIELA CAMPOLINA D PEIXOTO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
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Título: |
Polymorphisms and alternative splicing of the luteinizing hormone receptor of dairy cattle. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 15, n. 2, 2016. |
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Páginas: |
12 p. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract The aim of this study was to screen for variability in the luteinizing hormone/choriogonadotropin receptor (LHCGR) and to determine the occurrence of LHCGR mRNA isoforms in two dairy breeds of cattle. Granulosa cells from dominant ovarian follicles were recovered from 16 Gir and 16 Holstein cows, and total RNA was extracted. Complementary DNA was synthesized and PCR was used to generate amplicons for sequencing. Chromatograms were evaluated, and multiple sequences were aligned and analyzed for the presence of polymorphisms, allele frequency, polymorphic information content (PIC), and Hardy-Weinberg equilibrium (HWE). Twentyone single nucleotide polymorphisms (SNPs) were identified in LH receptor mRNA. Seventeen SNPs were identified in Gir cattle (seven exclusively), and 14 were found in Holstein cattle (four exclusively). Seven of the 21 polymorphisms found did not alter which amino acid was translated. Eight SNPs caused a change to an amino acid in a different chemical group. Classification of SNPs according to PIC values identified 12 as being highly informative in Gir cattle and five in Holstein. Eight SNPs deviated from HWE in Gir compared with 11 in Holstein, and eight in both breeds. Two isoforms were also identified, one in exon 1, which lacks 30 nucleotides beginning at position 118, and the other in exon 10. Taken together, these data show that LHCGR in dairy cattle breeds has a high frequency of polymorphism and exists in multiple isoforms resulting from alternative splicing. MenosAbstract The aim of this study was to screen for variability in the luteinizing hormone/choriogonadotropin receptor (LHCGR) and to determine the occurrence of LHCGR mRNA isoforms in two dairy breeds of cattle. Granulosa cells from dominant ovarian follicles were recovered from 16 Gir and 16 Holstein cows, and total RNA was extracted. Complementary DNA was synthesized and PCR was used to generate amplicons for sequencing. Chromatograms were evaluated, and multiple sequences were aligned and analyzed for the presence of polymorphisms, allele frequency, polymorphic information content (PIC), and Hardy-Weinberg equilibrium (HWE). Twentyone single nucleotide polymorphisms (SNPs) were identified in LH receptor mRNA. Seventeen SNPs were identified in Gir cattle (seven exclusively), and 14 were found in Holstein cattle (four exclusively). Seven of the 21 polymorphisms found did not alter which amino acid was translated. Eight SNPs caused a change to an amino acid in a different chemical group. Classification of SNPs according to PIC values identified 12 as being highly informative in Gir cattle and five in Holstein. Eight SNPs deviated from HWE in Gir compared with 11 in Holstein, and eight in both breeds. Two isoforms were also identified, one in exon 1, which lacks 30 nucleotides beginning at position 118, and the other in exon 10. Taken together, these data show that LHCGR in dairy cattle breeds has a high frequency of polymorphism and exists in multiple isoforms resulting from a... Mostrar Tudo |
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Palavras-Chave: |
LHCGR isoforms. |
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Thesaurus NAL: |
reproduction; zebu. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/155636/1/Cnpgl-2016-GMR-Polylmorphisms.pdf
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Marc: |
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001 2064058
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008 2016 bl uuuu u00u1 u #d
100 1 $aWOHLRES-VIANA, S.
245 $aPolymorphisms and alternative splicing of the luteinizing hormone receptor of dairy cattle.$h[electronic resource]
260 $c2016
300 $a12 p.
520 $aAbstract The aim of this study was to screen for variability in the luteinizing hormone/choriogonadotropin receptor (LHCGR) and to determine the occurrence of LHCGR mRNA isoforms in two dairy breeds of cattle. Granulosa cells from dominant ovarian follicles were recovered from 16 Gir and 16 Holstein cows, and total RNA was extracted. Complementary DNA was synthesized and PCR was used to generate amplicons for sequencing. Chromatograms were evaluated, and multiple sequences were aligned and analyzed for the presence of polymorphisms, allele frequency, polymorphic information content (PIC), and Hardy-Weinberg equilibrium (HWE). Twentyone single nucleotide polymorphisms (SNPs) were identified in LH receptor mRNA. Seventeen SNPs were identified in Gir cattle (seven exclusively), and 14 were found in Holstein cattle (four exclusively). Seven of the 21 polymorphisms found did not alter which amino acid was translated. Eight SNPs caused a change to an amino acid in a different chemical group. Classification of SNPs according to PIC values identified 12 as being highly informative in Gir cattle and five in Holstein. Eight SNPs deviated from HWE in Gir compared with 11 in Holstein, and eight in both breeds. Two isoforms were also identified, one in exon 1, which lacks 30 nucleotides beginning at position 118, and the other in exon 10. Taken together, these data show that LHCGR in dairy cattle breeds has a high frequency of polymorphism and exists in multiple isoforms resulting from alternative splicing.
650 $areproduction
650 $azebu
653 $aLHCGR isoforms
700 1 $aARASHIRO, E. K. N.
700 1 $aREIS, D. R. de L.
700 1 $aFERNANDES, L. E.
700 1 $aPEIXOTO, M. G. C. D.
700 1 $aMACHADO, M. A.
700 1 $aVIANA, J. H. M.
773 $tGenetics and Molecular Research$gv. 15, n. 2, 2016.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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