|
Registro Completo |
|
Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
|
Data corrente: |
24/05/2012 |
|
Data da última atualização: |
22/02/2016 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
COTTA, S. R; MOTA, F. F. da; TUPINAMBÁ, G.; ISHIDA, K.; ROZENTAL, S.; SILVA, D. O. e; SILVA, A. J. R. da; BIZZO, H. R.; ALVIANO, D. S.; ALVIANO, C. S.; SELDIN, L. |
|
Afiliação: |
SIMONE RAPOSO COTTA, UFRJ; FABIO FARIA DA MOTA, UFRJ; GLEISER TUPINAMBÁ, UFRJ; KELLY ISHIDA, UFRJ; SONIA ROZENTAL, UFRJ; DAVI OLIVEIRA E SILVA, UFRJ; ANTONIO JORGE RIBEIRO DA SILVA, UFRJ; HUMBERTO RIBEIRO BIZZO, CTAA; DANIELA SALES ALVIANO, UFRJ; CELUTA SALES ALVIANO, UFRJ; LUCY SELDIN, UFRJ. |
|
Título: |
Antimicrobial activity of Paenibacillus kribbensis POC 115 against the dermatophyte Trichophyton rubrum. |
|
Ano de publicação: |
2012 |
|
Fonte/Imprenta: |
World Journal of Microbiology and Biotechnology, v. 28, n. 3, p. 953-962, 2012. |
|
DOI: |
10.1007/s11274-011-0893-1 |
|
Idioma: |
Inglês |
|
Conteúdo: |
In a search for an antifungal substance with activity against the dermatophyte fungus Trichophyton rubrum, strain POC 115 was chosen among different Paenibacillus strains for its phenotypic and genetic characterization and for preliminary characterization of its antimicrobial substance. Strain POC 115 was identified as belonging to Paenibacillus kribbensis. Physico-chemical characterization of the antimicrobial substance showed that it was not stable during heat and organic solvents treatments, but its activity was preserved at a wide range of pH and after treatment with pronase E, trypsin and DNase I. The crude concentrated supernatant of POC 115 culture was partially purified and the fraction presenting antimicrobial activity was further analyzed by UPLC/Mass Spectrometry. Two peaks were observed at 2.02 (mass 1,207 D) and 2.71 (mass 1,014 D) min in the mass chromatogram. The antimicrobial substance produced by POC 115 was correlated to iturin family compounds based on a set of primers designed for the amplification of PKS operon in the POC 115 genome. As happens with the mode of action of the antibiotics of the iturin group, the AMS produced by POC 115 caused the disruption of cytoplasmic membrane of T. rubrum and the subsequent withdraw of the intracellular material. This is the first report on the production of antimicrobial substances in P. kribbensis, and it may be of great relevance as an alternative or supplementary substance to antifungal drugs currently used against dermatophytes. MenosIn a search for an antifungal substance with activity against the dermatophyte fungus Trichophyton rubrum, strain POC 115 was chosen among different Paenibacillus strains for its phenotypic and genetic characterization and for preliminary characterization of its antimicrobial substance. Strain POC 115 was identified as belonging to Paenibacillus kribbensis. Physico-chemical characterization of the antimicrobial substance showed that it was not stable during heat and organic solvents treatments, but its activity was preserved at a wide range of pH and after treatment with pronase E, trypsin and DNase I. The crude concentrated supernatant of POC 115 culture was partially purified and the fraction presenting antimicrobial activity was further analyzed by UPLC/Mass Spectrometry. Two peaks were observed at 2.02 (mass 1,207 D) and 2.71 (mass 1,014 D) min in the mass chromatogram. The antimicrobial substance produced by POC 115 was correlated to iturin family compounds based on a set of primers designed for the amplification of PKS operon in the POC 115 genome. As happens with the mode of action of the antibiotics of the iturin group, the AMS produced by POC 115 caused the disruption of cytoplasmic membrane of T. rubrum and the subsequent withdraw of the intracellular material. This is the first report on the production of antimicrobial substances in P. kribbensis, and it may be of great relevance as an alternative or supplementary substance to antifungal drugs currently used again... Mostrar Tudo |
|
Palavras-Chave: |
Dermatófitos patogênicos; Paenibacillus kribbensis; Substância antimicrobiana. |
|
Thesaurus Nal: |
Trichophyton rubrum. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
LEADER 02433naa a2200301 a 4500
001 1925383
005 2016-02-22
008 2012 bl uuuu u00u1 u #d
024 7 $a10.1007/s11274-011-0893-1$2DOI
100 1 $aCOTTA, S. R
245 $aAntimicrobial activity of Paenibacillus kribbensis POC 115 against the dermatophyte Trichophyton rubrum.
260 $c2012
520 $aIn a search for an antifungal substance with activity against the dermatophyte fungus Trichophyton rubrum, strain POC 115 was chosen among different Paenibacillus strains for its phenotypic and genetic characterization and for preliminary characterization of its antimicrobial substance. Strain POC 115 was identified as belonging to Paenibacillus kribbensis. Physico-chemical characterization of the antimicrobial substance showed that it was not stable during heat and organic solvents treatments, but its activity was preserved at a wide range of pH and after treatment with pronase E, trypsin and DNase I. The crude concentrated supernatant of POC 115 culture was partially purified and the fraction presenting antimicrobial activity was further analyzed by UPLC/Mass Spectrometry. Two peaks were observed at 2.02 (mass 1,207 D) and 2.71 (mass 1,014 D) min in the mass chromatogram. The antimicrobial substance produced by POC 115 was correlated to iturin family compounds based on a set of primers designed for the amplification of PKS operon in the POC 115 genome. As happens with the mode of action of the antibiotics of the iturin group, the AMS produced by POC 115 caused the disruption of cytoplasmic membrane of T. rubrum and the subsequent withdraw of the intracellular material. This is the first report on the production of antimicrobial substances in P. kribbensis, and it may be of great relevance as an alternative or supplementary substance to antifungal drugs currently used against dermatophytes.
650 $aTrichophyton rubrum
653 $aDermatófitos patogênicos
653 $aPaenibacillus kribbensis
653 $aSubstância antimicrobiana
700 1 $aMOTA, F. F. da
700 1 $aTUPINAMBÁ, G.
700 1 $aISHIDA, K.
700 1 $aROZENTAL, S.
700 1 $aSILVA, D. O. e
700 1 $aSILVA, A. J. R. da
700 1 $aBIZZO, H. R.
700 1 $aALVIANO, D. S.
700 1 $aALVIANO, C. S.
700 1 $aSELDIN, L.
773 $tWorld Journal of Microbiology and Biotechnology$gv. 28, n. 3, p. 953-962, 2012.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
|
