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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
03/08/2004 |
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Data da última atualização: |
05/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MOTA, F. F. da; GOMES, E. A.; PAIVA, E.; ROSADO, A. S.; SELDIN, L. |
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Afiliação: |
ELIANE APARECIDA GOMES, CNPMS; EMBRAPA-CNPMS. |
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Título: |
Use of rpoB gene analysis for identification of nitrogen-fixing Paenibacillus species as an alternative to the 16S rRNA gene. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Letters in Applied Microbiology, Oxford, v. 39, n. 1, p. 34-40, 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
Aim: To avoid the limitations of 16S rRNA-based phylogenetic analysis for Paenibacillus species, the usefulness of the RNA polymerase beta-subunit encoding gene (rpoB) was investigated as an alternative to the 16S rRNA gene for taxonomic studies. Methods and Results: Partial rpoB sequences were generated for the type strains of eight nitrogen-fixing Paenibacillus species. The presence of only one copy of rpoB in the genome of P. graminis strain RSA19T was demonstrated by denaturing gradient gel electrophoresis and hybridization assays. A comparative analysis of the sequences of the 16S rRNA and rpoB genes was performed and the eight species showed between 91·6-99·1% (16S rRNA) and 77·9-97·3% (rpoB) similarity, allowing a more accurate discrimination between the different species using the rpoB gene. Finally, 24 isolates from the rhizosphere of different cultivars of maize previously identified as Paenibacillus spp. were assigned correctly to one of the nitrogen-fixing species. Conclusions, Significance and Impact of the Study: The data obtained in this study indicate that rpoB is a powerful identification tool, which can be used for the correct discrimination of the nitrogen-fixing species of agricultural and industrial importance within the genus Paenibacillus. |
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Thesagro: |
Gene. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 01861naa a2200181 a 4500
001 1487746
005 2018-06-05
008 2004 bl uuuu u00u1 u #d
100 1 $aMOTA, F. F. da
245 $aUse of rpoB gene analysis for identification of nitrogen-fixing Paenibacillus species as an alternative to the 16S rRNA gene.$h[electronic resource]
260 $c2004
520 $aAim: To avoid the limitations of 16S rRNA-based phylogenetic analysis for Paenibacillus species, the usefulness of the RNA polymerase beta-subunit encoding gene (rpoB) was investigated as an alternative to the 16S rRNA gene for taxonomic studies. Methods and Results: Partial rpoB sequences were generated for the type strains of eight nitrogen-fixing Paenibacillus species. The presence of only one copy of rpoB in the genome of P. graminis strain RSA19T was demonstrated by denaturing gradient gel electrophoresis and hybridization assays. A comparative analysis of the sequences of the 16S rRNA and rpoB genes was performed and the eight species showed between 91·6-99·1% (16S rRNA) and 77·9-97·3% (rpoB) similarity, allowing a more accurate discrimination between the different species using the rpoB gene. Finally, 24 isolates from the rhizosphere of different cultivars of maize previously identified as Paenibacillus spp. were assigned correctly to one of the nitrogen-fixing species. Conclusions, Significance and Impact of the Study: The data obtained in this study indicate that rpoB is a powerful identification tool, which can be used for the correct discrimination of the nitrogen-fixing species of agricultural and industrial importance within the genus Paenibacillus.
650 $aGene
700 1 $aGOMES, E. A.
700 1 $aPAIVA, E.
700 1 $aROSADO, A. S.
700 1 $aSELDIN, L.
773 $tLetters in Applied Microbiology, Oxford$gv. 39, n. 1, p. 34-40, 2004.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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