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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Soja. |
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Data corrente: |
11/04/2002 |
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Data da última atualização: |
11/04/2002 |
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Autoria: |
MOSTASSO, L.; MOSTASSO, F. L.; DIAS, B. G.; VARGAS, M. A. T.; HUNGRIA, M. |
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Título: |
Selection of bean (Phaseolus vulgaris L.) rhizobial strains for the Brazilian Cerrados. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Field Crops Research, Amsterdam, v. 73, p. 121-132, 2002. |
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Idioma: |
Inglês
Português |
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Conteúdo: |
The common bean croop (Phaseolus vulgaris L.) occupies 5.5 million hectares in Brazil and provides about 30% of the populations protein needs. Yield remains low mainly due to limited availability of N and P, and to the often acid soil conditions. Surprisingly, there have been only very limited studies in Brazil in which the isolation and evaluation of efficient N fixing bean strains has been attempted. This paper reports the selection of bean rhizobia for the Cerrados, the savannas that represent one-fourth of Brazilian land. Two hundred strains were selected from large pink colored nodules collected in the Cerrados region and biological nitrogen fixation was evaluted under optimal (27/21º C, day/nigth) and high (37/21º C) temperature conditions. Thirty-six strains were selected and their nitrogen-fixing capacity and competitiveness further evaluated with black-seeded Negro Argel, and colored Carioca cultivars. One fifth of the strains showed low genetic stability of nodulation gene, but some strains were as or more competitive than the strains currently recommended for use in commercial bean inoculants in Brazil The superior performance of five strains was confirmed under field conditions and reinoculation in the second year increased bean yield. The DNA fingerprintings obtained by the ERIC-REP-PCR analyses indicated a high level of genetic diversity, and among the 36 strains, six different patterns of RFLP-PCR of the 16S rRNA gene region were detected. The 16S rRNA sequences of the most efficient and competitive strains were genetically similar to Rhizobium tropici, suggesting that further studies on inoculant strains in the hot and acid soils of the Brasilian Cerrados and Africa should emphasize this species. MenosThe common bean croop (Phaseolus vulgaris L.) occupies 5.5 million hectares in Brazil and provides about 30% of the populations protein needs. Yield remains low mainly due to limited availability of N and P, and to the often acid soil conditions. Surprisingly, there have been only very limited studies in Brazil in which the isolation and evaluation of efficient N fixing bean strains has been attempted. This paper reports the selection of bean rhizobia for the Cerrados, the savannas that represent one-fourth of Brazilian land. Two hundred strains were selected from large pink colored nodules collected in the Cerrados region and biological nitrogen fixation was evaluted under optimal (27/21º C, day/nigth) and high (37/21º C) temperature conditions. Thirty-six strains were selected and their nitrogen-fixing capacity and competitiveness further evaluated with black-seeded Negro Argel, and colored Carioca cultivars. One fifth of the strains showed low genetic stability of nodulation gene, but some strains were as or more competitive than the strains currently recommended for use in commercial bean inoculants in Brazil The superior performance of five strains was confirmed under field conditions and reinoculation in the second year increased bean yield. The DNA fingerprintings obtained by the ERIC-REP-PCR analyses indicated a high level of genetic diversity, and among the 36 strains, six different patterns of RFLP-PCR of the 16S rRNA gene region were detected. The 16S rRNA sequen... Mostrar Tudo |
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Palavras-Chave: |
Bean. |
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Thesagro: |
Cerrado; Feijão; Fixação de Nitrogênio; Phaseolus Vulgaris; Rhizobium. |
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Thesaurus Nal: |
nitrogen fixation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02437naa a2200253 a 4500
001 1564655
005 2002-04-11
008 2002 bl --- 0-- u #d
100 1 $aMOSTASSO, L.
245 $aSelection of bean (Phaseolus vulgaris L.) rhizobial strains for the Brazilian Cerrados.
260 $c2002
520 $aThe common bean croop (Phaseolus vulgaris L.) occupies 5.5 million hectares in Brazil and provides about 30% of the populations protein needs. Yield remains low mainly due to limited availability of N and P, and to the often acid soil conditions. Surprisingly, there have been only very limited studies in Brazil in which the isolation and evaluation of efficient N fixing bean strains has been attempted. This paper reports the selection of bean rhizobia for the Cerrados, the savannas that represent one-fourth of Brazilian land. Two hundred strains were selected from large pink colored nodules collected in the Cerrados region and biological nitrogen fixation was evaluted under optimal (27/21º C, day/nigth) and high (37/21º C) temperature conditions. Thirty-six strains were selected and their nitrogen-fixing capacity and competitiveness further evaluated with black-seeded Negro Argel, and colored Carioca cultivars. One fifth of the strains showed low genetic stability of nodulation gene, but some strains were as or more competitive than the strains currently recommended for use in commercial bean inoculants in Brazil The superior performance of five strains was confirmed under field conditions and reinoculation in the second year increased bean yield. The DNA fingerprintings obtained by the ERIC-REP-PCR analyses indicated a high level of genetic diversity, and among the 36 strains, six different patterns of RFLP-PCR of the 16S rRNA gene region were detected. The 16S rRNA sequences of the most efficient and competitive strains were genetically similar to Rhizobium tropici, suggesting that further studies on inoculant strains in the hot and acid soils of the Brasilian Cerrados and Africa should emphasize this species.
650 $anitrogen fixation
650 $aCerrado
650 $aFeijão
650 $aFixação de Nitrogênio
650 $aPhaseolus Vulgaris
650 $aRhizobium
653 $aBean
700 1 $aMOSTASSO, F. L.
700 1 $aDIAS, B. G.
700 1 $aVARGAS, M. A. T.
700 1 $aHUNGRIA, M.
773 $tField Crops Research, Amsterdam$gv. 73, p. 121-132, 2002.
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Registro original: |
Embrapa Cerrados (CPAC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
24/10/2003 |
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Data da última atualização: |
24/10/2003 |
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Autoria: |
MARIN, V. A.; TEIXEIRA, K. R. S. dos; BALDANI, J. I. |
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Título: |
Characterization of amplified polymerase chain reaction glnB and nifH gene fragments of nitrogen-fixing Burkholderia species. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Letters in Applied Microbiology, Oxford, v. 36, p. 77-82, 2003. |
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Idioma: |
Inglês |
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Conteúdo: |
Aims: To clone and sequence polymerase chain reaction (PCR)-amplified glnB and nifH genes of the nitrogen- fixing bacteria Burkholderia brasilensis strain M130, B. tropicalis strain PPe8 and B. kururiensis strain KP23. Methods and Resu1ts: The glnB and nifH gene fragments were amplified by PCR using universal degenerated primers. A very high percentage of similarity for the nifH (100%) and glnB (96%) genes was observed between strains M130 and KP23. A similarity of 100% for the nifH gene was also observed between strains M130 and PPe8. However, the identity for the glnB gene was 98% and the similarity 88%. The phylogenetic tree of the nifH gene showed a very high degree of similarity to the 16S rDNA gene. Conc1usions: The nitrogen-fixing bacteria of the Burkholderia genus formed a cluster separated from the other species of the genus mainly when the nifH rather than the glnB gene was used to construct the phylogenetic tree. Significance and Impact of the Study: Knowledge of the nifH and glnB gene sequences of B. brasilensis, B. tropicalis and B. kururiensis will support new studies on the diversity of these diazotrophs in natural environments.
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Palavras-Chave: |
BNF; FBN; Fixação biológica de nitrogênio; Nitrogen fixation biology. |
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Thesagro: |
Bactéria. |
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Thesaurus NAL: |
Burkholderia. |
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Categoria do assunto: |
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Marc: |
LEADER 01831naa a2200217 a 4500
001 1624610
005 2003-10-24
008 2003 bl --- 0-- u #d
100 1 $aMARIN, V. A.
245 $aCharacterization of amplified polymerase chain reaction glnB and nifH gene fragments of nitrogen-fixing Burkholderia species.
260 $c2003
520 $aAims: To clone and sequence polymerase chain reaction (PCR)-amplified glnB and nifH genes of the nitrogen- fixing bacteria Burkholderia brasilensis strain M130, B. tropicalis strain PPe8 and B. kururiensis strain KP23. Methods and Resu1ts: The glnB and nifH gene fragments were amplified by PCR using universal degenerated primers. A very high percentage of similarity for the nifH (100%) and glnB (96%) genes was observed between strains M130 and KP23. A similarity of 100% for the nifH gene was also observed between strains M130 and PPe8. However, the identity for the glnB gene was 98% and the similarity 88%. The phylogenetic tree of the nifH gene showed a very high degree of similarity to the 16S rDNA gene. Conc1usions: The nitrogen-fixing bacteria of the Burkholderia genus formed a cluster separated from the other species of the genus mainly when the nifH rather than the glnB gene was used to construct the phylogenetic tree. Significance and Impact of the Study: Knowledge of the nifH and glnB gene sequences of B. brasilensis, B. tropicalis and B. kururiensis will support new studies on the diversity of these diazotrophs in natural environments.
650 $aBurkholderia
650 $aBactéria
653 $aBNF
653 $aFBN
653 $aFixação biológica de nitrogênio
653 $aNitrogen fixation biology
700 1 $aTEIXEIRA, K. R. S. dos
700 1 $aBALDANI, J. I.
773 $tLetters in Applied Microbiology, Oxford$gv. 36, p. 77-82, 2003.
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