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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
15/09/2010 |
Data da última atualização: |
02/05/2019 |
Autoria: |
ALEXANDRE, T. M.; RIBEIRO, Z. M. A.; CRAVEIRO, S. R.; CUNHA, F.; FONSECA, I. C. B.; MOSCARDI, F.; CASTRO, M. E. B. |
Afiliação: |
TALITA M. ALEXANDRE, CNPSo - UFPR; ZILDA MARIA DE ARAUJO RIBEIRO, CENARGEN; SALUANA R. CRAVEIRO, CENARGEN - UNB; FABIANE CUNHA, CNPSo - UFPR; INES CRISTINA B. FONSECA, UEL; FLÁVIO MOSCARDI, UEL; MARIA ELITA B. CASTRO, UEL. |
Título: |
Evaluation of seven viral isolates as potential biocontrol agents against Pseudoplusia includens (Lepidoptera: Noctuidae) caterpillars. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Journal of Invertebrate Pathology, Amsterdan, v. 105, n. 1, p. 98-104, Sept. 2010. |
DOI: |
10.1016/j.jip.2010.05.015 |
Idioma: |
Português |
Conteúdo: |
The caterpillar Pseudoplusia includens (Walker, 1857) (Lepidoptera, Noctuidae), known as soybean looper, is a pest that has recently assumed greater importance in soybean in Brazil. Isolates of nucleopolyhedroviruses (NPVs) of this pest have been identified from cotton in Guatemala and soybean farms in Brazil, providing an interesting perspective of potential use of viral insecticide against the insect in lieu to chemical insecticides. With the objective to contribute to the characterization studies of this virus, morphological and molecular analyses and biological activity were carried out with seven P. includens viral isolates (I-A to I-G). Electron microscopy of viral samples, purified from macerated infected larvae, showed particles with typical morphology of the Baculoviridae family, genus Alphabaculovirus (Nucleopolyhedrovirus ? NPV) presenting virions with only a single nucleocapsid per envelope (SNPV) occluded in a protein matrix, forming occlusion bodies (OB). This virus was then classified as P. includens single nucleopolyhedrovirus (PsinSNPV). OB particles analyzed in SDS?polyacrylamide gel showed an intense band corresponding in size to NPV polyhedrin protein. DNA restriction profiles of the PsinSNPV isolates showed differences in the fragment size and number suggesting the existence of genotypic variants, except between I-E and I-F profiles that were similar. Among the isolates tested for infectivity against P. includens, I-A, I-E and I-F were the most virulent. Survival times (ST50) varied according to viral concentration, with significant differences among isolates for the three higher concentrations. MenosThe caterpillar Pseudoplusia includens (Walker, 1857) (Lepidoptera, Noctuidae), known as soybean looper, is a pest that has recently assumed greater importance in soybean in Brazil. Isolates of nucleopolyhedroviruses (NPVs) of this pest have been identified from cotton in Guatemala and soybean farms in Brazil, providing an interesting perspective of potential use of viral insecticide against the insect in lieu to chemical insecticides. With the objective to contribute to the characterization studies of this virus, morphological and molecular analyses and biological activity were carried out with seven P. includens viral isolates (I-A to I-G). Electron microscopy of viral samples, purified from macerated infected larvae, showed particles with typical morphology of the Baculoviridae family, genus Alphabaculovirus (Nucleopolyhedrovirus ? NPV) presenting virions with only a single nucleocapsid per envelope (SNPV) occluded in a protein matrix, forming occlusion bodies (OB). This virus was then classified as P. includens single nucleopolyhedrovirus (PsinSNPV). OB particles analyzed in SDS?polyacrylamide gel showed an intense band corresponding in size to NPV polyhedrin protein. DNA restriction profiles of the PsinSNPV isolates showed differences in the fragment size and number suggesting the existence of genotypic variants, except between I-E and I-F profiles that were similar. Among the isolates tested for infectivity against P. includens, I-A, I-E and I-F were the most virulent.... Mostrar Tudo |
Thesagro: |
Baculovirus; Controle biológico; Lagarta; Pseudoplusia Includens; Pupa; Soja; Virus. |
Thesaurus Nal: |
Biological control; Insect control; Insect larvae; Soybeans; Virulence. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02660naa a2200349 a 4500 001 1862327 005 2019-05-02 008 2010 bl uuuu u00u1 u #d 024 7 $a10.1016/j.jip.2010.05.015$2DOI 100 1 $aALEXANDRE, T. M. 245 $aEvaluation of seven viral isolates as potential biocontrol agents against Pseudoplusia includens (Lepidoptera$bNoctuidae) caterpillars. 260 $c2010 520 $aThe caterpillar Pseudoplusia includens (Walker, 1857) (Lepidoptera, Noctuidae), known as soybean looper, is a pest that has recently assumed greater importance in soybean in Brazil. Isolates of nucleopolyhedroviruses (NPVs) of this pest have been identified from cotton in Guatemala and soybean farms in Brazil, providing an interesting perspective of potential use of viral insecticide against the insect in lieu to chemical insecticides. With the objective to contribute to the characterization studies of this virus, morphological and molecular analyses and biological activity were carried out with seven P. includens viral isolates (I-A to I-G). Electron microscopy of viral samples, purified from macerated infected larvae, showed particles with typical morphology of the Baculoviridae family, genus Alphabaculovirus (Nucleopolyhedrovirus ? NPV) presenting virions with only a single nucleocapsid per envelope (SNPV) occluded in a protein matrix, forming occlusion bodies (OB). This virus was then classified as P. includens single nucleopolyhedrovirus (PsinSNPV). OB particles analyzed in SDS?polyacrylamide gel showed an intense band corresponding in size to NPV polyhedrin protein. DNA restriction profiles of the PsinSNPV isolates showed differences in the fragment size and number suggesting the existence of genotypic variants, except between I-E and I-F profiles that were similar. Among the isolates tested for infectivity against P. includens, I-A, I-E and I-F were the most virulent. Survival times (ST50) varied according to viral concentration, with significant differences among isolates for the three higher concentrations. 650 $aBiological control 650 $aInsect control 650 $aInsect larvae 650 $aSoybeans 650 $aVirulence 650 $aBaculovirus 650 $aControle biológico 650 $aLagarta 650 $aPseudoplusia Includens 650 $aPupa 650 $aSoja 650 $aVirus 700 1 $aRIBEIRO, Z. M. A. 700 1 $aCRAVEIRO, S. R. 700 1 $aCUNHA, F. 700 1 $aFONSECA, I. C. B. 700 1 $aMOSCARDI, F. 700 1 $aCASTRO, M. E. B. 773 $tJournal of Invertebrate Pathology, Amsterdan$gv. 105, n. 1, p. 98-104, Sept. 2010.
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Embrapa Soja (CNPSO) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
22/06/2010 |
Data da última atualização: |
02/03/2011 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
ABREU, V. M. N.; ABREU, P. G. de; TOMAZELLI, I. L.; CESTONARO, T.; CONCEIÇÃO, V. da; MORÉS, R. |
Afiliação: |
VALERIA MARIA NASCIMENTO ABREU, CNPSA; PAULO GIOVANNI DE ABREU, CNPSA; INAIARA LETÍCIA TOMAZELLI, UNC; TAIANA CESTONARO, UNC; VANESSA DA CONCEIÇÃO, UNC; RÚBIA MORÉS, UNC. |
Título: |
Distribuição da temperatura e umidade do ar no sentido transversal em aviário de postura automatizado com sistema de nebulização. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
In: CONGRESSO APA PRODUÇÃO E COMERCIALIZAÇÃO DE OVOS, 8., 2010, São Pedro. Anais 2010: as portas de um futuro promissor São Paulo: APA, 2010. p. 314-316. |
Idioma: |
Português |
Notas: |
Projeto/Plano de Ação: 03.07.52.000-02 |
Palavras-Chave: |
Experimento; Nebulização. |
Thesagro: |
Aviário; Temperatura. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00827naa a2200229 a 4500 001 1855812 005 2011-03-02 008 2010 bl uuuu u00u1 u #d 100 1 $aABREU, V. M. N. 245 $aDistribuição da temperatura e umidade do ar no sentido transversal em aviário de postura automatizado com sistema de nebulização. 260 $c2010 500 $aProjeto/Plano de Ação: 03.07.52.000-02 650 $aAviário 650 $aTemperatura 653 $aExperimento 653 $aNebulização 700 1 $aABREU, P. G. de 700 1 $aTOMAZELLI, I. L. 700 1 $aCESTONARO, T. 700 1 $aCONCEIÇÃO, V. da 700 1 $aMORÉS, R. 773 $tIn: CONGRESSO APA PRODUÇÃO E COMERCIALIZAÇÃO DE OVOS, 8., 2010, São Pedro. Anais 2010: as portas de um futuro promissor São Paulo: APA, 2010. p. 314-316.
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