Data/hora: 07/06/2024 20:23:07

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	26/11/1999	
Data da última atualização: 	15/05/2023	
Autoria: 	MIRANDA, M.; MOTOMURA, T.; IKEDA, F.; OHGAWARA, T.; SAITO, W.; ENDO, T.; OMURA, M.; MORIGUCHI, T.	
Título: 	Somatic hybrids obtained by fusion between Poncirus trifoliata (2x) and fortunella hindsii (4x) protoplasts	
Ano de publicação: 	1997	
Fonte/Imprenta: 	Plant Cell Reports, v.16, p.401-405, 1997.	
Idioma: 	Inglês	
Conteúdo: 	Somatic hybrids were obtained by the symmetric fusion of embryogenic callus cells from tetraploid `Mame' kumquat [Fortunella hindsii (Champ.) Swing.] and mesophyll cells from diploid trifoliate orange [Poncirus trifoliata (L.) Raf.]. Southern blot analysis of three regenerants revealed that they carried specific rDNA fragments from both fusion partners, thereby confirming their hybridity. In contrast, mitochondrial DNA (mtDNA) and chloroblast DNA (cpDNA) were unidirectionally transmitted from the callus parent without any evidence of recombination. No differences in the restriction fragment patterns of rDNA, mtDNA or cpDNA could be detected among the regenerants. Flow cytometry showed that two regenerants were hexaploids, as expected, but that one was pentaploid, probably due to elimination of chromosomes prior to the regeneration of this plant.	
Palavras-Chave: 	Citros; Eletrofusao; Eliminacao de cromossomos; Poliplaides.	
Thesaurus Nal: 	chromosome elimination; Citrus; electrofusion; flow cytometry; polyploidy.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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