|
|
Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
09/06/2017 |
Data da última atualização: |
09/06/2017 |
Autoria: |
MORI, M.; MATSUKURA, S.; KAWAKURA, K.; TAMAOKI, B.-I. |
Título: |
In-vitro synthesis of androgen from pregnenolone in the testes of the goat (Capra hircus) and identification of 5-pregnene-3beta,17alpha,20alpha-triol as an intermediate in the metabolic pathway of pregnenolone. |
Ano de publicação: |
1980 |
Fonte/Imprenta: |
Journal of Endocrinology, v. 84, n. 2, p. 381-390, Feb. 1980. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: When [4(-14)C]pregnenolone was aerobically incubated in vitro in the presence of NAD+ and NADPH with cell-free homogenates of testicular tissue of adult domestic goats (Capra hircus), progesterone, 17alpha-hydroxypregnenolone, 17alpha-hydroxyprogesterone, 17alpha,20alpha-dihydroxy-4-pregnen-3-one, dehydroepiandrosterone, androstenedione and testosterone were identified as its known metabolites. Time-course studies on this metabolism showed that the production of 17alpha,20alpha-dihydroxy-4-pregnen-3-one and testosterone constantly increased up to the end of incubation, suggesting that these are both end-products of pregnenolone metabolism in this system. The other metabolites behaved as intermediates and were ultimately converted, in part, to testosterone by the testicular homogenates, indicating that testosterone was synthesized through both 4-ene and 5-ene-pathways. Furthermore, besides these metabolites, 5-pregnene-3beta,17alpha,20alpha-triol was also identified as an intermediary metabolite, formed from pregnenolone through 17alpha-hydroxypregnenolone in the presence of NADPH, and further convertible into 17alpha,20alpha-dihydroxy-4-pregnen-3-one by the microsomal fraction and into 17alpha-hydroxypregnenolone by the cytosol fraction in the presence of NAD+ and NADP+. It was not, however, significantly transformed into C19-steroids. Furthermore, 17alpha,20alpha-dihydroxy-4-pregnen-3-one, which was formed either from 5-pregnene-3beta,17alpha,20alpha-triol or from 17alpha-hydroxyprogesterone, remained almost unchanged without conversion to C19-steroids when incubated with the caprine testicular homogenates. MenosAbstract: When [4(-14)C]pregnenolone was aerobically incubated in vitro in the presence of NAD+ and NADPH with cell-free homogenates of testicular tissue of adult domestic goats (Capra hircus), progesterone, 17alpha-hydroxypregnenolone, 17alpha-hydroxyprogesterone, 17alpha,20alpha-dihydroxy-4-pregnen-3-one, dehydroepiandrosterone, androstenedione and testosterone were identified as its known metabolites. Time-course studies on this metabolism showed that the production of 17alpha,20alpha-dihydroxy-4-pregnen-3-one and testosterone constantly increased up to the end of incubation, suggesting that these are both end-products of pregnenolone metabolism in this system. The other metabolites behaved as intermediates and were ultimately converted, in part, to testosterone by the testicular homogenates, indicating that testosterone was synthesized through both 4-ene and 5-ene-pathways. Furthermore, besides these metabolites, 5-pregnene-3beta,17alpha,20alpha-triol was also identified as an intermediary metabolite, formed from pregnenolone through 17alpha-hydroxypregnenolone in the presence of NADPH, and further convertible into 17alpha,20alpha-dihydroxy-4-pregnen-3-one by the microsomal fraction and into 17alpha-hydroxypregnenolone by the cytosol fraction in the presence of NAD+ and NADP+. It was not, however, significantly transformed into C19-steroids. Furthermore, 17alpha,20alpha-dihydroxy-4-pregnen-3-one, which was formed either from 5-pregnene-3beta,17alpha,20alpha-triol or from... Mostrar Tudo |
Palavras-Chave: |
17-alpha-Hydroxypregnenolone; 20-Hydroxysteroid Dehydrogenases; 3-Hydroxysteroid Dehydrogenases. |
Thesaurus Nal: |
Androgens; Androstenedione; Biosynthesis; Goats; pregnenolone; Progesterone; Testosterone. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02564naa a2200277 a 4500 001 2070660 005 2017-06-09 008 1980 bl uuuu u00u1 u #d 100 1 $aMORI, M. 245 $aIn-vitro synthesis of androgen from pregnenolone in the testes of the goat (Capra hircus) and identification of 5-pregnene-3beta,17alpha,20alpha-triol as an intermediate in the metabolic pathway of pregnenolone. 260 $c1980 520 $aAbstract: When [4(-14)C]pregnenolone was aerobically incubated in vitro in the presence of NAD+ and NADPH with cell-free homogenates of testicular tissue of adult domestic goats (Capra hircus), progesterone, 17alpha-hydroxypregnenolone, 17alpha-hydroxyprogesterone, 17alpha,20alpha-dihydroxy-4-pregnen-3-one, dehydroepiandrosterone, androstenedione and testosterone were identified as its known metabolites. Time-course studies on this metabolism showed that the production of 17alpha,20alpha-dihydroxy-4-pregnen-3-one and testosterone constantly increased up to the end of incubation, suggesting that these are both end-products of pregnenolone metabolism in this system. The other metabolites behaved as intermediates and were ultimately converted, in part, to testosterone by the testicular homogenates, indicating that testosterone was synthesized through both 4-ene and 5-ene-pathways. Furthermore, besides these metabolites, 5-pregnene-3beta,17alpha,20alpha-triol was also identified as an intermediary metabolite, formed from pregnenolone through 17alpha-hydroxypregnenolone in the presence of NADPH, and further convertible into 17alpha,20alpha-dihydroxy-4-pregnen-3-one by the microsomal fraction and into 17alpha-hydroxypregnenolone by the cytosol fraction in the presence of NAD+ and NADP+. It was not, however, significantly transformed into C19-steroids. Furthermore, 17alpha,20alpha-dihydroxy-4-pregnen-3-one, which was formed either from 5-pregnene-3beta,17alpha,20alpha-triol or from 17alpha-hydroxyprogesterone, remained almost unchanged without conversion to C19-steroids when incubated with the caprine testicular homogenates. 650 $aAndrogens 650 $aAndrostenedione 650 $aBiosynthesis 650 $aGoats 650 $apregnenolone 650 $aProgesterone 650 $aTestosterone 653 $a17-alpha-Hydroxypregnenolone 653 $a20-Hydroxysteroid Dehydrogenases 653 $a3-Hydroxysteroid Dehydrogenases 700 1 $aMATSUKURA, S. 700 1 $aKAWAKURA, K. 700 1 $aTAMAOKI, B.-I. 773 $tJournal of Endocrinology$gv. 84, n. 2, p. 381-390, Feb. 1980.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
08/01/2014 |
Data da última atualização: |
11/01/2019 |
Tipo da produção científica: |
Documentos |
Autoria: |
TONIETTO, J.; ZANUS, M. C.; FALCADE, I.; GUERRA, C. C. |
Afiliação: |
JORGE TONIETTO, CNPUV; MAURO CELSO ZANUS, CNPUV; IVANIRA FALCADE, UCS; CELITO CRIVELLARO GUERRA, CNPUV. |
Título: |
O regulamento de uso da indicação geográfica Altos Montes: vinhos finos tranquilos e espumantes. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Bento Gonçalves: Embrapa Uva e Vinho, 2013. |
Páginas: |
36 p. |
Descrição Física: |
il. |
Série: |
(Embrapa Uva e Vinho. Documentos, 85). |
ISSN: |
1516-8107 |
Idioma: |
Português |
Conteúdo: |
A Indicação de Procedência Altos Montes. O Regulamento de Uso da Indicação de Procedência Altos Montes. A Operacionalização do Regulamento de Uso da Indicação de Procedência Altos Montes |
Palavras-Chave: |
Altos Montes; Espumante; Indicação geográfica; Rio Grande do Sul; Zoneamento. |
Thesagro: |
Geografia; Legislação; Uva; Vinho; Viticultura. |
Categoria do assunto: |
-- |
URL: |
https://www.cnpuv.embrapa.br/publica/documentos/doc085.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/94888/1/doc085.pdf
|
Marc: |
LEADER 01040nam a2200301 a 4500 001 1975276 005 2019-01-11 008 2013 bl uuuu u0uu1 u #d 022 $a1516-8107 100 1 $aTONIETTO, J. 245 $aO regulamento de uso da indicação geográfica Altos Montes$bvinhos finos tranquilos e espumantes.$h[electronic resource] 260 $aBento Gonçalves: Embrapa Uva e Vinho$c2013 300 $a36 p.$cil. 490 $a(Embrapa Uva e Vinho. Documentos, 85). 520 $aA Indicação de Procedência Altos Montes. O Regulamento de Uso da Indicação de Procedência Altos Montes. A Operacionalização do Regulamento de Uso da Indicação de Procedência Altos Montes 650 $aGeografia 650 $aLegislação 650 $aUva 650 $aVinho 650 $aViticultura 653 $aAltos Montes 653 $aEspumante 653 $aIndicação geográfica 653 $aRio Grande do Sul 653 $aZoneamento 700 1 $aZANUS, M. C. 700 1 $aFALCADE, I. 700 1 $aGUERRA, C. C.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Uva e Vinho (CNPUV) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|