Data/hora: 05/06/2024 05:30:35

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 14/10/1999
Data da última atualização: | 14/10/1999
Autoria: | POWELL, W.; MORGANTE, M.; ANDRE, C.; HANAFEY, M.; VOGEL, J.; TINGEY, S.; RAFALSKI, A.
Afiliação: | Biotechnology Research, Agricultural Products Department, E. I. du Pont de Nemours and Co (Inc.) P. O. Box 80402 Experimental Station.
Título: | The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis
Ano de publicação: | 1996
Fonte/Imprenta: | Milecualar-Breeding, v.2, n.3, p.225-238, 1996
Idioma: | Inglês
Conteúdo: | The utility of RFLP (restriction fragment length polymorphism), RAPD (random-amplified polymorphic DNA), AFLP (amplified fragment length polymorphism) and SSR (simple sequence repeat, microsatellite) markers in soybean germplasm analysis eas determined by evaluating information content (expected heterozygosity), number of loci simultaneously analyzed per experiment (multiplex ratio) and effectiveness in assessing relationships between accessions. SSR markers have the highest expected heterozygosity (0.60), while AFLP markers have the highest effective multiplex ratio (19). A single prameter, defined as the marker index, which is the product of expected heterozygosity and multiplex ratio, may be used to evaluate overall utility of a marker system. A comparison of genetic similarity matrices revealed that, if the comparison involved both cultivated (Glycine max) and wild soybean (Glycine soja) accessions, estimates based on RFLPs, AFLPs and SSRs are highly correlated, indicating congruence between these assays.
Palavras-Chave: | AFLP; Glycine; Microsatellite; RAPD; RFLP; Simple sequence repeat polymorphism; Soybean; SSR.
Thesaurus Nal: | germplasm; polymorphism.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (17374-1) - ADD - SP - -- - S11544 -  - S11544 - DIS