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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
01/07/2004 |
Data da última atualização: |
01/07/2004 |
Autoria: |
GONZALEZ-BULNES, A.; GARCIA-GARCIA, R. M.; SANTIAGO-MORENO, J.; LOPEZ-SEBASTIAN, A.; COCERO, M. J. |
Título: |
Effect of follicular status on superovulatory response in ewes is influenced by presence of corpus luteum at first FSH dose. |
Ano de publicação: |
2002 |
Fonte/Imprenta: |
Theriogenology, v. 58, n. 8, p. 1607-1614, 2002. |
Idioma: |
Inglês |
Conteúdo: |
The present study was developed to assess possible effects on ovulatory response and embryo yields arising from the presence of a corpus luteum (CL) at the time of initiation of the progestagen treatment used in superovulatory protocols in sheep. In breeding season, estrus was synchronized in 25 Manchega ewes using 40 mg FGA sponges for 14 days, together with a single dose of 125 ug of cloprostenol on Day 12, with Day 0 as day of progestagen insertion. Superovulatory treatment consisted of eight decreasing doses (1.5 x 3 ml, 1.25 x 2 ml, and 1 x 3 ml) of OvagenTM twice daily from 60 h before to 24 h after sponge removal. The presence or absence of corpora lutea was assessed by transrectal ultrasonography at progestagen insertion and at first FSH dose. Number and size of all follicles >=2 mm were also evaluated at first FSH dose. The number of corpora lutea and the number and viability of recovered embryos in response to the treatment were evaluated 7 days after sponge removal. No significant effect on ovarian response of the presence of a CL at sponge insertion in 21 of the 25 ewes (84%) was detected. However, ewes with a CL at first FSH dose (16 ewes, 64%) yielded a higher number of transferable embryos (7.2 +- 1.4 versus 2.7 +- 0.7, P < 0.05), since the embryo degeneration rate was increased in sheep without a CL (42.5% versus 12.7%, P < 0.01). Analysis of possible effects derived from the presence of a large presumptively dominant follicle (>=6 mm) at first FSH dose showed that both recovery and viability rates were lowest (P < 0.05) in ewes bearing a large follicle in the absence of a CL (40.5 and 50.6%, respectively), and highest in ewes that did not show a large follicle but in which a CL was present (73.9 and 85.2%). The final number of transferable embryos was very different between groups (10.2 versus 1.8, P < 0.01). These results indicate that the number and quality of embryos obtained from superovulated ewes is affected by the presence of a CL prior to the first FSH dose (i.e. by the stage of the estrous cycle at progestagen insertion) and also by an interaction with suppressive effects from large dominant follicles. This finding suggests the existence of some effects on follicular population prior to the FSH treatment that may compromise follicle and oocyte developmental competence. It seems reasonable to hypothesize that superovulatory yields wuold be increased by beginning the treatment during the early-luteal phase of the estrous cycle, allowing for the presence of a CL along with the progestagen treatment. MenosThe present study was developed to assess possible effects on ovulatory response and embryo yields arising from the presence of a corpus luteum (CL) at the time of initiation of the progestagen treatment used in superovulatory protocols in sheep. In breeding season, estrus was synchronized in 25 Manchega ewes using 40 mg FGA sponges for 14 days, together with a single dose of 125 ug of cloprostenol on Day 12, with Day 0 as day of progestagen insertion. Superovulatory treatment consisted of eight decreasing doses (1.5 x 3 ml, 1.25 x 2 ml, and 1 x 3 ml) of OvagenTM twice daily from 60 h before to 24 h after sponge removal. The presence or absence of corpora lutea was assessed by transrectal ultrasonography at progestagen insertion and at first FSH dose. Number and size of all follicles >=2 mm were also evaluated at first FSH dose. The number of corpora lutea and the number and viability of recovered embryos in response to the treatment were evaluated 7 days after sponge removal. No significant effect on ovarian response of the presence of a CL at sponge insertion in 21 of the 25 ewes (84%) was detected. However, ewes with a CL at first FSH dose (16 ewes, 64%) yielded a higher number of transferable embryos (7.2 +- 1.4 versus 2.7 +- 0.7, P < 0.05), since the embryo degeneration rate was increased in sheep without a CL (42.5% versus 12.7%, P < 0.01). Analysis of possible effects derived from the presence of a large presumptively dominant follicle (>=6 mm) at first FSH dose showe... Mostrar Tudo |
Palavras-Chave: |
Folículo. |
Thesagro: |
Corpo Lúteo; Embrião; Ovário; Ovelha; Ovino; Reprodução Animal; Superovulação. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03337naa a2200265 a 4500 001 1530632 005 2004-07-01 008 2002 bl uuuu u00u1 u #d 100 1 $aGONZALEZ-BULNES, A. 245 $aEffect of follicular status on superovulatory response in ewes is influenced by presence of corpus luteum at first FSH dose. 260 $c2002 520 $aThe present study was developed to assess possible effects on ovulatory response and embryo yields arising from the presence of a corpus luteum (CL) at the time of initiation of the progestagen treatment used in superovulatory protocols in sheep. In breeding season, estrus was synchronized in 25 Manchega ewes using 40 mg FGA sponges for 14 days, together with a single dose of 125 ug of cloprostenol on Day 12, with Day 0 as day of progestagen insertion. Superovulatory treatment consisted of eight decreasing doses (1.5 x 3 ml, 1.25 x 2 ml, and 1 x 3 ml) of OvagenTM twice daily from 60 h before to 24 h after sponge removal. The presence or absence of corpora lutea was assessed by transrectal ultrasonography at progestagen insertion and at first FSH dose. Number and size of all follicles >=2 mm were also evaluated at first FSH dose. The number of corpora lutea and the number and viability of recovered embryos in response to the treatment were evaluated 7 days after sponge removal. No significant effect on ovarian response of the presence of a CL at sponge insertion in 21 of the 25 ewes (84%) was detected. However, ewes with a CL at first FSH dose (16 ewes, 64%) yielded a higher number of transferable embryos (7.2 +- 1.4 versus 2.7 +- 0.7, P < 0.05), since the embryo degeneration rate was increased in sheep without a CL (42.5% versus 12.7%, P < 0.01). Analysis of possible effects derived from the presence of a large presumptively dominant follicle (>=6 mm) at first FSH dose showed that both recovery and viability rates were lowest (P < 0.05) in ewes bearing a large follicle in the absence of a CL (40.5 and 50.6%, respectively), and highest in ewes that did not show a large follicle but in which a CL was present (73.9 and 85.2%). The final number of transferable embryos was very different between groups (10.2 versus 1.8, P < 0.01). These results indicate that the number and quality of embryos obtained from superovulated ewes is affected by the presence of a CL prior to the first FSH dose (i.e. by the stage of the estrous cycle at progestagen insertion) and also by an interaction with suppressive effects from large dominant follicles. This finding suggests the existence of some effects on follicular population prior to the FSH treatment that may compromise follicle and oocyte developmental competence. It seems reasonable to hypothesize that superovulatory yields wuold be increased by beginning the treatment during the early-luteal phase of the estrous cycle, allowing for the presence of a CL along with the progestagen treatment. 650 $aCorpo Lúteo 650 $aEmbrião 650 $aOvário 650 $aOvelha 650 $aOvino 650 $aReprodução Animal 650 $aSuperovulação 653 $aFolículo 700 1 $aGARCIA-GARCIA, R. M. 700 1 $aSANTIAGO-MORENO, J. 700 1 $aLOPEZ-SEBASTIAN, A. 700 1 $aCOCERO, M. J. 773 $tTheriogenology$gv. 58, n. 8, p. 1607-1614, 2002.
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Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
01/09/2009 |
Data da última atualização: |
25/05/2018 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
BOREGAS, K. G. B.; MENDES, S. M.; FERMINO, T. C.; WAQUIL, J. M. |
Afiliação: |
Katia G. Brasil Boregas, CNPMS; SIMONE MARTINS MENDES, CNPMS; Talita C. Firmino, CNPMS; JOSE MAGID WAQUIL, CNPMS. |
Título: |
Avaliação da sobrevivência de predadores de Spodoptera frugiperda (J. E. Smith, 1797) alimentados com lagartas desenvolvidas em milho-BT e não-BT. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 11., 2009, Bento Gonçalves. Tecnologia e conservação ambiental: resumos. [S.l.]: Sociedade Entomológica do Brasil: IRGA: Unisinos: Fiocruz, 2009. |
Descrição Física: |
1 CD-ROM. |
Idioma: |
Português |
Palavras-Chave: |
Manejo de pragas; Transgênicos. |
Thesagro: |
Controle Biológico. |
Thesaurus NAL: |
Insecta. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/56944/1/Avaliacao-sobrevivencia.pdf
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Marc: |
LEADER 00789nam a2200193 a 4500 001 1492060 005 2018-05-25 008 2009 bl uuuu u00u1 u #d 100 1 $aBOREGAS, K. G. B. 245 $aAvaliação da sobrevivência de predadores de Spodoptera frugiperda (J. E. Smith, 1797) alimentados com lagartas desenvolvidas em milho-BT e não-BT.$h[electronic resource] 260 $aIn: SIMPÓSIO DE CONTROLE BIOLÓGICO, 11., 2009, Bento Gonçalves. Tecnologia e conservação ambiental: resumos. [S.l.]: Sociedade Entomológica do Brasil: IRGA: Unisinos: Fiocruz$c2009 300 $c1 CD-ROM. 650 $aInsecta 650 $aControle Biológico 653 $aManejo de pragas 653 $aTransgênicos 700 1 $aMENDES, S. M. 700 1 $aFERMINO, T. C. 700 1 $aWAQUIL, J. M.
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