| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Hortaliças; Embrapa Unidades Centrais. |
|
Data corrente: |
01/04/1997 |
|
Data da última atualização: |
01/04/1997 |
|
Autoria: |
GUEDES, A. C.; MOREIRA, H. M.; MENEZES, J. E. |
|
Afiliação: |
EMBRAPA-CNPH, Brasilia, DF. |
|
Título: |
Producao e importacao de sementes de hortalicas no Brasil - 1981-1985. |
|
Ano de publicação: |
1988 |
|
Fonte/Imprenta: |
Brasilia: EMBRAPA-CNPH, 1988. |
|
Páginas: |
141p. |
|
Série: |
(EMBRAPA-CNPH. Documentos, 2). |
|
Idioma: |
Português |
|
Conteúdo: |
O presente trabalho é um contribuição do Centro Nacional de Pesquisa de Hortaliças (CNPH) da EMBRAPA à ações de vários setores para o desenvolvimento da olericultura nacional. Mesmo tratando-se de uma coleta quatittiva de dados, muitas informações importantes foram obtidas, sendo a principal delas a revelação do quadro da realidade do setor e o seu consequente registro histórico. A utilização destes dados por pesquisadores, professores, jprodutore, importadores e comerciantes de sementes será o termômetro que marcará a importância deste trabalho que ora é colacado à disposição de todos. |
|
Palavras-Chave: |
Brasil; Import; Production; Seed; Vegetable. |
|
Thesagro: |
Hortaliça; Importação; Produção; Semente. |
|
Thesaurus Nal: |
Brazil. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01300nam a2200277 a 4500
001 1756908
005 1997-04-01
008 1988 bl uuuu 00u1 u #d
100 1 $aGUEDES, A. C.
245 $aProducao e importacao de sementes de hortalicas no Brasil - 1981-1985.
260 $aBrasilia: EMBRAPA-CNPH$c1988
300 $a141p.
490 $a(EMBRAPA-CNPH. Documentos, 2).
520 $aO presente trabalho é um contribuição do Centro Nacional de Pesquisa de Hortaliças (CNPH) da EMBRAPA à ações de vários setores para o desenvolvimento da olericultura nacional. Mesmo tratando-se de uma coleta quatittiva de dados, muitas informações importantes foram obtidas, sendo a principal delas a revelação do quadro da realidade do setor e o seu consequente registro histórico. A utilização destes dados por pesquisadores, professores, jprodutore, importadores e comerciantes de sementes será o termômetro que marcará a importância deste trabalho que ora é colacado à disposição de todos.
650 $aBrazil
650 $aHortaliça
650 $aImportação
650 $aProdução
650 $aSemente
653 $aBrasil
653 $aImport
653 $aProduction
653 $aSeed
653 $aVegetable
700 1 $aMOREIRA, H. M.
700 1 $aMENEZES, J. E.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Hortaliças (CNPH) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
06/11/2017 |
|
Data da última atualização: |
06/11/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
CARVALHO, M. J. S.; OLIVEIRA, E. J. de; SOUZA, A. da S.; PEREIRA, J. S.; DIAMANTINO, M. S. A. S.; OLIVEIRA, S. A. S. de. |
|
Afiliação: |
M. J. S. CARVALHO; EDER JORGE DE OLIVEIRA, CNPMF; ANTONIO DA SILVA SOUZA, CNPMF; J. S. PEREIRA; M. S .A. S. DIAMANTINO; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF. |
|
Título: |
Cleaning cassava genotypes infected with cassava frogskin disease via in vitro shoot tip culture. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
Genetics and Molecular Research, v. 16, n.2, 2017. |
|
Série: |
1676-5680 |
|
Idioma: |
Inglês |
|
Conteúdo: |
This study aimed to develop a methodology for eliminating cassava frogskin disease (CFSD) from in vitro shoot tip culture by associating thermotherapy and tetracycline. Cuttings from different accessions (BGM0232, BGM0315, BGM0464, BGM584, BGM0841, and BGM1342), infected with CFSD according to visual inspection of the disease symptoms, were used for cleaning. To verify the absence of other diseases, the plants were indexed for Cassava common mosaic virus - CsCMV (by ELISA) and Cassava vein mosaic virus - CsVMV (by polymerase chain reaction, PCR), proving that the accessions were free of these viruses, except for BGM0315 and BGM0464, which were infected with CsVMV. Subsequently, the cuttings were submitted to different tetracycline concentrations for 3 min, and then subjected to thermotherapy under different temperatures (35°, 38°, 40°, 45°, and 55°C). Shoots of 2 cm were harvested, and their surfaces were sterilized in a laminar flow chamber. Subsequently, the shoot tips of different sizes were removed (0.2, 0.4, 0.5, and 1.0 mm) for inoculation in a culture medium with tetracycline at the same concentrations in which the cuttings were dipped. After 60 days of cultivation, the explants were transferred to a multiplication medium without antibiotics. Thirty days after the transfer, the viability of the regenerated plants was evaluated, which were then acclimatized for 70 days in a greenhouse and transferred to the field. After 7 months, a visual analysis of the symptomatic roots and a PCR analysis were held to prove the elimination of CFSD and CsVMV from the accessions infected with these viruses (BGM0315 and BGM0464), respectively. Most of the treatments resulted in 100% cleaning of CFSD-infected plants. From accessions that were also infected with CsVMV, only 2% of the plants remained infected, also demonstrating the cleaning efficiency of this protocol for this disease. MenosThis study aimed to develop a methodology for eliminating cassava frogskin disease (CFSD) from in vitro shoot tip culture by associating thermotherapy and tetracycline. Cuttings from different accessions (BGM0232, BGM0315, BGM0464, BGM584, BGM0841, and BGM1342), infected with CFSD according to visual inspection of the disease symptoms, were used for cleaning. To verify the absence of other diseases, the plants were indexed for Cassava common mosaic virus - CsCMV (by ELISA) and Cassava vein mosaic virus - CsVMV (by polymerase chain reaction, PCR), proving that the accessions were free of these viruses, except for BGM0315 and BGM0464, which were infected with CsVMV. Subsequently, the cuttings were submitted to different tetracycline concentrations for 3 min, and then subjected to thermotherapy under different temperatures (35°, 38°, 40°, 45°, and 55°C). Shoots of 2 cm were harvested, and their surfaces were sterilized in a laminar flow chamber. Subsequently, the shoot tips of different sizes were removed (0.2, 0.4, 0.5, and 1.0 mm) for inoculation in a culture medium with tetracycline at the same concentrations in which the cuttings were dipped. After 60 days of cultivation, the explants were transferred to a multiplication medium without antibiotics. Thirty days after the transfer, the viability of the regenerated plants was evaluated, which were then acclimatized for 70 days in a greenhouse and transferred to the field. After 7 months, a visual analysis of the symptomatic ro... Mostrar Tudo |
|
Thesagro: |
Mandioca. |
|
Thesaurus NAL: |
cassava. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02559naa a2200217 a 4500
001 2078909
005 2017-11-06
008 2017 bl --- 0-- u #d
100 1 $aCARVALHO, M. J. S.
245 $aCleaning cassava genotypes infected with cassava frogskin disease via in vitro shoot tip culture.$h[electronic resource]
260 $c2017
490 $a1676-5680
520 $aThis study aimed to develop a methodology for eliminating cassava frogskin disease (CFSD) from in vitro shoot tip culture by associating thermotherapy and tetracycline. Cuttings from different accessions (BGM0232, BGM0315, BGM0464, BGM584, BGM0841, and BGM1342), infected with CFSD according to visual inspection of the disease symptoms, were used for cleaning. To verify the absence of other diseases, the plants were indexed for Cassava common mosaic virus - CsCMV (by ELISA) and Cassava vein mosaic virus - CsVMV (by polymerase chain reaction, PCR), proving that the accessions were free of these viruses, except for BGM0315 and BGM0464, which were infected with CsVMV. Subsequently, the cuttings were submitted to different tetracycline concentrations for 3 min, and then subjected to thermotherapy under different temperatures (35°, 38°, 40°, 45°, and 55°C). Shoots of 2 cm were harvested, and their surfaces were sterilized in a laminar flow chamber. Subsequently, the shoot tips of different sizes were removed (0.2, 0.4, 0.5, and 1.0 mm) for inoculation in a culture medium with tetracycline at the same concentrations in which the cuttings were dipped. After 60 days of cultivation, the explants were transferred to a multiplication medium without antibiotics. Thirty days after the transfer, the viability of the regenerated plants was evaluated, which were then acclimatized for 70 days in a greenhouse and transferred to the field. After 7 months, a visual analysis of the symptomatic roots and a PCR analysis were held to prove the elimination of CFSD and CsVMV from the accessions infected with these viruses (BGM0315 and BGM0464), respectively. Most of the treatments resulted in 100% cleaning of CFSD-infected plants. From accessions that were also infected with CsVMV, only 2% of the plants remained infected, also demonstrating the cleaning efficiency of this protocol for this disease.
650 $acassava
650 $aMandioca
700 1 $aOLIVEIRA, E. J. de
700 1 $aSOUZA, A. da S.
700 1 $aPEREIRA, J. S.
700 1 $aDIAMANTINO, M. S. A. S.
700 1 $aOLIVEIRA, S. A. S. de
773 $tGenetics and Molecular Research$gv. 16, n.2, 2017.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
