Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
01/02/2016 |
|
Data da última atualização: |
01/02/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 1 |
|
Autoria: |
CASTRO, R. de J. C.; SOUZA SOBRINHO, F. de; GAMA, M. A. S. da; SATO, R. T.; LIMA, L. L.; OLIVEIRA, M. A. L. de. |
|
Afiliação: |
Renata de Jesus Coelho Castro, UFJF; FAUSTO DE SOUZA SOBRINHO, CNPGL; MARCO ANTONIO SUNDFELD DA GAMA, CNPGL; Renata Takabayashi Sato, UFJF; Larissa Lavorato Lima, UFJF; Marcone Augusto Leal de Oliveira, UFJF. |
|
Título: |
A Rapid Method for Determination of the Main Conjugated Linoleic Acid Precursors (C18:2 n-6 and C18:3 n-3) in Forage by Capillary Zone Electrophoresis with Ultraviolet Detection Using Gas Chromatography with Flame Ionization Detection as a Comparative Method. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
Journal of AOAC International, v. 98, n. 6, p. 1591-1597, 2015. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: A rapid method has been proposed for determination of the main conjugated linoleic acid precursors such as linoleic (C18:2 n-6) and linolenic (C18:3 n-3) acids in forages by capillary zone electrophoresis (CZE) with direct UV detection at 200 nm. Among the fatty acids found in forages, C18:2 n-6 and C18:3 n-3 have received particular attention due to their roles as precursors for the synthesis of conjugated linoleic acid, a class of health-enhancing compounds that is predominantly found in dairy products. The electrolyte background consisted of 12.0 mmol/L tetraborate buffer (pH 9.2) added to 12.0 mmol/L Brij 35(®), 17% acetonitrile, and 33% methanol. Under the optimized conditions, the baseline separation of C18:2 n-6 and C18:3 n-3 was achieved within 4 min. The CZE-UV method was compared to GC with a flame ionization detector, which is the American Oil Chemists' Society (AOCS 996.06) official method for fatty acid analysis. The methods did not show any evidence of significant differences within 95% confidence interval (P > 0.05). The CZE-UV method was successfully applied to the analysis of 80 genotypes of Brachiaria ruzizienses clones submitted to a genetic improvement program in agricultural research. |
|
Palavras-Chave: |
CLA; CZE-UV method; Fatty acids in forages. |
|
Thesaurus NAL: |
conjugated linoleic acid. |
|
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
|
Marc: |
LEADER 02109naa a2200229 a 4500
001 2035820
005 2024-02-01
008 2015 bl uuuu u00u1 u #d
100 1 $aCASTRO, R. de J. C.
245 $aA Rapid Method for Determination of the Main Conjugated Linoleic Acid Precursors (C18$b2 n-6 and C18:3 n-3) in Forage by Capillary Zone Electrophoresis with Ultraviolet Detection Using Gas Chromatography with Flame Ionization Detection as a Comparative Method.$h[electronic resource]
260 $c2015
520 $aAbstract: A rapid method has been proposed for determination of the main conjugated linoleic acid precursors such as linoleic (C18:2 n-6) and linolenic (C18:3 n-3) acids in forages by capillary zone electrophoresis (CZE) with direct UV detection at 200 nm. Among the fatty acids found in forages, C18:2 n-6 and C18:3 n-3 have received particular attention due to their roles as precursors for the synthesis of conjugated linoleic acid, a class of health-enhancing compounds that is predominantly found in dairy products. The electrolyte background consisted of 12.0 mmol/L tetraborate buffer (pH 9.2) added to 12.0 mmol/L Brij 35(®), 17% acetonitrile, and 33% methanol. Under the optimized conditions, the baseline separation of C18:2 n-6 and C18:3 n-3 was achieved within 4 min. The CZE-UV method was compared to GC with a flame ionization detector, which is the American Oil Chemists' Society (AOCS 996.06) official method for fatty acid analysis. The methods did not show any evidence of significant differences within 95% confidence interval (P > 0.05). The CZE-UV method was successfully applied to the analysis of 80 genotypes of Brachiaria ruzizienses clones submitted to a genetic improvement program in agricultural research.
650 $aconjugated linoleic acid
653 $aCLA
653 $aCZE-UV method
653 $aFatty acids in forages
700 1 $aSOUZA SOBRINHO, F. de
700 1 $aGAMA, M. A. S. da
700 1 $aSATO, R. T.
700 1 $aLIMA, L. L.
700 1 $aOLIVEIRA, M. A. L. de
773 $tJournal of AOAC International$gv. 98, n. 6, p. 1591-1597, 2015.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
