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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
05/12/2012 |
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Data da última atualização: |
05/12/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MORAES, R. M.; MELO, I. S. de; SUMYANTO, J.; CHANDRA, S.; JOSHI, V. |
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Afiliação: |
RITA M. MORAES, University of Mississippi; ITAMAR SOARES DE MELO, CNPMA; JOKO SUMYANTO, University of Mississippi; SUMAN CHANDRA, University of Mississippi; VAISHALI JOSHI, University of Mississippi. |
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Título: |
Bacterial community associated with autotrophic and heterotrophic cultures of medicinal plant smallanthus sonchifolius (Yacon). |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
American Journal of Plant Sciences, v. 3, n. 10, p. 1382-1389, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
Several unfruitful attempts to grow axenic shoot cultures of Smallanthus sonchifolius, also known as yacon, were made before healthy shoots grew in association with bacteria on half strength Murashigue and Skoog media supplemented with 2.2 µM benzylaminopurine. Twenty-one bacterial isolates were obtained from in vitro S. sonchifolius plantlets, eight of these isolates were identified as Flavimonas oryzihabitans, Curtobacterium pusillum, Sphingomonas paucimobilis, and Microbacterium imperiale. These microorganisms produced indole acetic acid (IAA) at amounts varying between 8.89 to 47.45 µg/mL, reason for being classified as plant growth promoting bacteria (PGPB). The results show that buds associated with bacteria cultured on sucrose free media produced 3.77 new roots measuring 18.33 cm in length after a 30-day growing period. In contrast, buds growing on sucrose supplemented media, the number of roots induced was higher (6.67 to 14 roots/explant) but shorter in length, 4.67 to 5.83 cm. During plant acclimatization to soil, photosynthesis and water efficiency were measured showing that the plants were healthy and vigorous. A slightly higher rate of photosynthesis and water use efficiency was recorded in the plants produced on heterotrophic conditions as compared to plants grown in sucrose free media. Plants adapted well in the soil demonstrating that the PGPB community associated to S. sonchifolius in shoot cultures was not harmful to plant production. The purpose of this study was to demonstrate that the bacteria associated with in vitro S. sonchifolius shoot cultures was not the result of microbial contamination, but rather from symbiotic associations that extended from cultivation in the greenhouse, to culture and back to soil. This is the first report to show that autotrophic cultures may represent a viable alternative to grow healthy plants without eliminating beneficial bacteria associated with the host. MenosSeveral unfruitful attempts to grow axenic shoot cultures of Smallanthus sonchifolius, also known as yacon, were made before healthy shoots grew in association with bacteria on half strength Murashigue and Skoog media supplemented with 2.2 µM benzylaminopurine. Twenty-one bacterial isolates were obtained from in vitro S. sonchifolius plantlets, eight of these isolates were identified as Flavimonas oryzihabitans, Curtobacterium pusillum, Sphingomonas paucimobilis, and Microbacterium imperiale. These microorganisms produced indole acetic acid (IAA) at amounts varying between 8.89 to 47.45 µg/mL, reason for being classified as plant growth promoting bacteria (PGPB). The results show that buds associated with bacteria cultured on sucrose free media produced 3.77 new roots measuring 18.33 cm in length after a 30-day growing period. In contrast, buds growing on sucrose supplemented media, the number of roots induced was higher (6.67 to 14 roots/explant) but shorter in length, 4.67 to 5.83 cm. During plant acclimatization to soil, photosynthesis and water efficiency were measured showing that the plants were healthy and vigorous. A slightly higher rate of photosynthesis and water use efficiency was recorded in the plants produced on heterotrophic conditions as compared to plants grown in sucrose free media. Plants adapted well in the soil demonstrating that the PGPB community associated to S. sonchifolius in shoot cultures was not harmful to plant production. The purpose of this st... Mostrar Tudo |
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Palavras-Chave: |
Çido indolacético; IAA; Yacon. |
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Thesagro: |
Bactéria; Planta medicinal. |
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Thesaurus Nal: |
Endophytes; Indole acetic acid; Micropropagation; Smallanthus sonchifolius. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/71469/1/2012AP37.pdf
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Marc: |
LEADER 02774naa a2200277 a 4500
001 1941315
005 2012-12-05
008 2012 bl uuuu u00u1 u #d
100 1 $aMORAES, R. M.
245 $aBacterial community associated with autotrophic and heterotrophic cultures of medicinal plant smallanthus sonchifolius (Yacon).$h[electronic resource]
260 $c2012
520 $aSeveral unfruitful attempts to grow axenic shoot cultures of Smallanthus sonchifolius, also known as yacon, were made before healthy shoots grew in association with bacteria on half strength Murashigue and Skoog media supplemented with 2.2 µM benzylaminopurine. Twenty-one bacterial isolates were obtained from in vitro S. sonchifolius plantlets, eight of these isolates were identified as Flavimonas oryzihabitans, Curtobacterium pusillum, Sphingomonas paucimobilis, and Microbacterium imperiale. These microorganisms produced indole acetic acid (IAA) at amounts varying between 8.89 to 47.45 µg/mL, reason for being classified as plant growth promoting bacteria (PGPB). The results show that buds associated with bacteria cultured on sucrose free media produced 3.77 new roots measuring 18.33 cm in length after a 30-day growing period. In contrast, buds growing on sucrose supplemented media, the number of roots induced was higher (6.67 to 14 roots/explant) but shorter in length, 4.67 to 5.83 cm. During plant acclimatization to soil, photosynthesis and water efficiency were measured showing that the plants were healthy and vigorous. A slightly higher rate of photosynthesis and water use efficiency was recorded in the plants produced on heterotrophic conditions as compared to plants grown in sucrose free media. Plants adapted well in the soil demonstrating that the PGPB community associated to S. sonchifolius in shoot cultures was not harmful to plant production. The purpose of this study was to demonstrate that the bacteria associated with in vitro S. sonchifolius shoot cultures was not the result of microbial contamination, but rather from symbiotic associations that extended from cultivation in the greenhouse, to culture and back to soil. This is the first report to show that autotrophic cultures may represent a viable alternative to grow healthy plants without eliminating beneficial bacteria associated with the host.
650 $aEndophytes
650 $aIndole acetic acid
650 $aMicropropagation
650 $aSmallanthus sonchifolius
650 $aBactéria
650 $aPlanta medicinal
653 $aÇido indolacético
653 $aIAA
653 $aYacon
700 1 $aMELO, I. S. de
700 1 $aSUMYANTO, J.
700 1 $aCHANDRA, S.
700 1 $aJOSHI, V.
773 $tAmerican Journal of Plant Sciences$gv. 3, n. 10, p. 1382-1389, 2012.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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| Registros recuperados : 199 | |
| 41. |  | MORAES, R. P. de; FIGUEIREDO, P. J. M.; ZAPPAROLI, I. D.; SILVA, F. C. da. Compostagem para Londrina, PR: definição de um sistema de gestão de resíduos sólidos domésticos. In: CONGRESSO DA SOCIEDADE BRASILEIRA DE ECONOMIA, ADMINISTRAÇÃO E SOCIOLOGIA RURAL, 53., 2015, João Pessoa. Agropecuária, meio ambiente e desenvolvimento: anais eletrônicos. João Pessoa: Sober, 2015. Não paginado. Sober 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 46. | | NAZARÉ, R. F. R. de; ALMEIDA, M. das G. C.; MORAES, R. R. Processamento, enriquecimento protéico e conservação do néctar de tucumã. In: SIMPÓSIO DO TRÓPICO ÚMIDO, 1., 1984, Belém, PA. Resumos. Belém, PA: EMBRAPA-CPATU, 1984. p. 265. (EMBRAPA-CPATU. Documentos, 31).| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 47. | | NAZARÉ, R. F. R. de; ALMEIDA, M. das G. C.; MORAES, R. R. Processamento, enriquecimento protéico e conservação do néctar de tucumã. In: SIMPÓSIO DO TRÓPICO ÚMIDO, 1., 1984, Belém, PA. Anais. Belém, PA: EMBRAPA-CPATU, 1986. v. 4, p. 253-263. (EMBRAPA-CPATU. Documentos, 36). v.4 Culturas perenes.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 50. | | BULBOVAS, P.; SOUZA, S. R. de; MORAES, R. M. de; LUIZÃO, F.; ARTAXO, P. Plântulas de soja 'Tracajá' expostas ao ozônio sob condições controladas. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 42, n. 5, p. 641-646, maio 2007 Título em inglês: Soybean 'Tracajá' seedlings exposed to ozone under controlled conditions.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 55. | | BERTAGNOLLI, P. F.; COSTAMILAN, L. M.; MORAES, R. M. A. de; EICHELBERGER, L. BRS Charrua RR: cultivar de soja indicada para o sul do estado de Mato Grosso do Sul. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 29., 2007, Campo Grande, MS. Resumos... Londrina: Embrapa Soja, 2007. p. 91-92. (Embrapa Soja. Documentos, 287). Organizado por Odilon Ferreira Saraiva, Regina Maria Villas Boas de Campos Leite, Simone Ery Grosskopf.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Trigo. |
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| 56. | | BERTAGNOLLI, P. F.; MORAES, R. M. A. de; COSTAMILAN, L. M.; EICHELBERGER, L. BRS Pampa RR: cultivar de soja indicada para o sul do estado de Mato Grosso do Sul. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 29., 2007, Campo Grande, MS. Resumos... Londrina: Embrapa Soja, 2007. p. 93-94. (Embrapa Soja. Documentos, 287). Organizado por Odilon Ferreira Saraiva, Regina Maria Villas Boas de Campos Leite, Simone Ery Grosskopf.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Trigo. |
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| 58. | | MORAES, R. M. A. de; BERTAGNOLLI, P. F.; COSTAMILAN, L. M.; EICHELBERGER, L.; PITOL, C. Melhoramento de soja na Embrapa Trigo, safra 2006/07. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO SUL, 35., 2007, Santa Maria. Ata e resumos... Santa Maria: Universidade Federal de Santa Maria, 2007. p. 47. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Trigo. |
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| 59. | | MORAES, R. M. A. de; BERTAGNOLLI, P. F.; COSTAMILAN, L. M.; PITOL, C.; SCHNEIDER, S. Melhoramento de soja na Embrapa Trigo, safra agrícola 2005/06. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO SUL, 34., 2006, Pelotas. Ata e resumos... Pelotas: Embrapa Clima Temperado, 2006. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| Registros recuperados : 199 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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