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 | Acesso ao texto completo restrito à biblioteca da Embrapa Rondônia. Para informações adicionais entre em contato com cpafro.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Clima Temperado; Embrapa Rondônia. |
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Data corrente: |
14/06/2021 |
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Data da última atualização: |
15/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MORAES, F. P. de; D'AVILA, C. A.; OLIVEIRA, F. C. de; CASTRO, N. A. de; VIEIRA, A. D.; SCHNEIDER, A.; PFEIFER, L. F. M.; PEGORARO, L. M. C.; FERREIRA, R.; FERST, J. G.; ROVANI, M. T.; CORREA, M. N.; GONÇALVES, P. B. D.; LUCIA JUNIOR, T.; GASPERIN, B. G. |
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Afiliação: |
FABIANE PEREIRA DE MORAES, Federal University of Pelotas; CAMILA AMARAL D'AVILA, Federal University of Pelotas; FERNANDO CAETANO DE OLIVEIRA, Federal University of Pelotas; NATALIA ÁVILA DE CASTRO, Federal University of Pelotas; ARNALDO DINIZ VIEIRA, Federal University of Pelotas; AUGUSTO SCHNEIDER, Federal University of Pelotas; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO; LIGIA MARGARETH CANTARELLI PEGORARO, CPACT; ROGERIO FERREIRA, Santa Catarina State University; JULIANA GERMANO FERST, Federal University of Santa Maria; MONIQUE TOMAZELE ROVANI, Federal University of Rio Grande do Sul; MARCIO NUNES CORREA, Federal University of Pelotas; PAULO BAYARD DIAS GONÇALVES, Federal University of Santa Maria / Federal University of Pampa; THOMAZ LUCIA JUINOR, Federal University of Pelotas; BERNARDO GARZIERA GASPERIN, Federal University of Pelotas. |
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Título: |
Prostaglandin F2a regulation and function during ovulation and luteinization in cows. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Theriogenology, v. 171, p. 30-37, Sept. 2021. |
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ISSN: |
0093-691X |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2021.05.008 |
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Idioma: |
Inglês |
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Conteúdo: |
Although prostaglandins are important in the ovulation process, a precise role for prostaglandin F2a (PGF) has not been elucidated. This study aimed to evaluate the regulation of PGF receptor mRNA (PTGFR) in granulosa cells and the local effect of PGF on ovulation and luteinization. In Experiment 1, using samples collected in vivo before (Day 2), during (Day 3) and after (Day 4) follicular deviation, expression of PTGFR in bovine granulosa cells was more abundant in the dominant follicle after deviation than in subordinates (P < 0.05). However, the expression of PTGFR was not regulated (P ¼ 0.1) in preovulatory follicles at different time-points (0, 3, 6, 12 and 24 h) after ovulation induction with GnRH. In Experiment 2, to assess the role of systemic PGF treatment on luteinization and vascularization of preovulatory follicles, flunixin meglumine (FM), a nonsteroidal anti-inflammatory drug, was used to inhibit endogenous prostaglandin synthesis. Cows with preovulatory follicles were induced to ovulate with GnRH (0 h) and allocated to three groups: Control, with no further treatment; FM, treated with 2.2 mg/kg FM im 17 h after GnRH treatment; and FM þ PGF, treated with FM 17 h after GnRH, followed by 25 mg dinoprost tromethamine (PGF) 23 h after GnRH treatment. FM injection was able to reduce the concentration of PGF in the follicular fluid (FF) (P < 0.001). However, contrary to our hypothesis, color Doppler ultrasound evaluations revealed decreased vascular flow in FM þ PGF group (P < 0.05), and no effect of the treatments on intrafollicular P4 and E2 concentrations 24 h after GnRH. The prostaglandin metabolite (PGFM) concentrations in the FF were greater in cows receiving systemic PGF (P < 0.001), which prompted us to further check its role on ovulation. Therefore, in Experiment 3, in a final attempt to demonstrate the local effect of PGF on ovulation, cows with preovulatory follicles received an intrafollicular injection (IFI) of PBS (Control) or 100 ng/mL purified PGF (PGF group). PGF treatment did not affect the time of ovulation after IFI (66 ± 6.4 and 63 ± 8.5 h for control and PGF, respectively; P > 0.05), further suggesting that it has no direct effect in the ovulatory process. Based on our findings, we concluded that FM decreased PGF synthesis within the follicle, whereas PGF treatment decreased follicular vascularization. In addition, the in vivo model of intrafollicular injection evidenced that PGF alone is not able to locally induce ovulation. MenosAlthough prostaglandins are important in the ovulation process, a precise role for prostaglandin F2a (PGF) has not been elucidated. This study aimed to evaluate the regulation of PGF receptor mRNA (PTGFR) in granulosa cells and the local effect of PGF on ovulation and luteinization. In Experiment 1, using samples collected in vivo before (Day 2), during (Day 3) and after (Day 4) follicular deviation, expression of PTGFR in bovine granulosa cells was more abundant in the dominant follicle after deviation than in subordinates (P < 0.05). However, the expression of PTGFR was not regulated (P ¼ 0.1) in preovulatory follicles at different time-points (0, 3, 6, 12 and 24 h) after ovulation induction with GnRH. In Experiment 2, to assess the role of systemic PGF treatment on luteinization and vascularization of preovulatory follicles, flunixin meglumine (FM), a nonsteroidal anti-inflammatory drug, was used to inhibit endogenous prostaglandin synthesis. Cows with preovulatory follicles were induced to ovulate with GnRH (0 h) and allocated to three groups: Control, with no further treatment; FM, treated with 2.2 mg/kg FM im 17 h after GnRH treatment; and FM þ PGF, treated with FM 17 h after GnRH, followed by 25 mg dinoprost tromethamine (PGF) 23 h after GnRH treatment. FM injection was able to reduce the concentration of PGF in the follicular fluid (FF) (P < 0.001). However, contrary to our hypothesis, color Doppler ultrasound evaluations revealed decreased vascular flow in FM þ PGF ... Mostrar Tudo |
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Palavras-Chave: |
Ação anti-inflamatória; F2a; Luteinização. |
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Thesagro: |
Ovulação; Prostaglandina; Reprodução Animal; Vaca. |
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Thesaurus Nal: |
Animal reproduction; Anti-inflammatory activity; Cows; Luteinization; Ovulation; Prostaglandins. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
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024 7 $ahttps://doi.org/10.1016/j.theriogenology.2021.05.008$2DOI
100 1 $aMORAES, F. P. de
245 $aProstaglandin F2a regulation and function during ovulation and luteinization in cows.$h[electronic resource]
260 $c2021
520 $aAlthough prostaglandins are important in the ovulation process, a precise role for prostaglandin F2a (PGF) has not been elucidated. This study aimed to evaluate the regulation of PGF receptor mRNA (PTGFR) in granulosa cells and the local effect of PGF on ovulation and luteinization. In Experiment 1, using samples collected in vivo before (Day 2), during (Day 3) and after (Day 4) follicular deviation, expression of PTGFR in bovine granulosa cells was more abundant in the dominant follicle after deviation than in subordinates (P < 0.05). However, the expression of PTGFR was not regulated (P ¼ 0.1) in preovulatory follicles at different time-points (0, 3, 6, 12 and 24 h) after ovulation induction with GnRH. In Experiment 2, to assess the role of systemic PGF treatment on luteinization and vascularization of preovulatory follicles, flunixin meglumine (FM), a nonsteroidal anti-inflammatory drug, was used to inhibit endogenous prostaglandin synthesis. Cows with preovulatory follicles were induced to ovulate with GnRH (0 h) and allocated to three groups: Control, with no further treatment; FM, treated with 2.2 mg/kg FM im 17 h after GnRH treatment; and FM þ PGF, treated with FM 17 h after GnRH, followed by 25 mg dinoprost tromethamine (PGF) 23 h after GnRH treatment. FM injection was able to reduce the concentration of PGF in the follicular fluid (FF) (P < 0.001). However, contrary to our hypothesis, color Doppler ultrasound evaluations revealed decreased vascular flow in FM þ PGF group (P < 0.05), and no effect of the treatments on intrafollicular P4 and E2 concentrations 24 h after GnRH. The prostaglandin metabolite (PGFM) concentrations in the FF were greater in cows receiving systemic PGF (P < 0.001), which prompted us to further check its role on ovulation. Therefore, in Experiment 3, in a final attempt to demonstrate the local effect of PGF on ovulation, cows with preovulatory follicles received an intrafollicular injection (IFI) of PBS (Control) or 100 ng/mL purified PGF (PGF group). PGF treatment did not affect the time of ovulation after IFI (66 ± 6.4 and 63 ± 8.5 h for control and PGF, respectively; P > 0.05), further suggesting that it has no direct effect in the ovulatory process. Based on our findings, we concluded that FM decreased PGF synthesis within the follicle, whereas PGF treatment decreased follicular vascularization. In addition, the in vivo model of intrafollicular injection evidenced that PGF alone is not able to locally induce ovulation.
650 $aAnimal reproduction
650 $aAnti-inflammatory activity
650 $aCows
650 $aLuteinization
650 $aOvulation
650 $aProstaglandins
650 $aOvulação
650 $aProstaglandina
650 $aReprodução Animal
650 $aVaca
653 $aAção anti-inflamatória
653 $aF2a
653 $aLuteinização
700 1 $aD'AVILA, C. A.
700 1 $aOLIVEIRA, F. C. de
700 1 $aCASTRO, N. A. de
700 1 $aVIEIRA, A. D.
700 1 $aSCHNEIDER, A.
700 1 $aPFEIFER, L. F. M.
700 1 $aPEGORARO, L. M. C.
700 1 $aFERREIRA, R.
700 1 $aFERST, J. G.
700 1 $aROVANI, M. T.
700 1 $aCORREA, M. N.
700 1 $aGONÇALVES, P. B. D.
700 1 $aLUCIA JUNIOR, T.
700 1 $aGASPERIN, B. G.
773 $tTheriogenology$gv. 171, p. 30-37, Sept. 2021.
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Registro original: |
Embrapa Rondônia (CPAF-RO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
09/08/2023 |
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Data da última atualização: |
09/08/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
VILARINHO, A. A.; PIRES, J. L. F.; ANTONIAZZI, N. |
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Afiliação: |
ALOISIO ALCANTARA VILARINHO, CNPT; JOAO LEONARDO FERNANDES PIRES, CNPT; NOEMIR ANTONIAZZI, Fundação Agrária de Pesquisa Agropecuária/Guarapuava. |
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Título: |
Ensaios VCU 3 do programa de melhoramento de cevada cervejeira da Embrapa em 2019. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
In: REUNIÃO NACIONAL DE PESQUISA DE CEVADA, 33., 2023, Passo Fundo. Anais... Passo Fundo: Acervus, 2023. |
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Páginas: |
p. 65-69. |
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Idioma: |
Português |
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Conteúdo: |
Resumo: O presente trabalho teve por objetivo avaliar os ensaios de valor de cultivo e uso (VCU 3) de cevada cervejeira da Embrapa, em 2019. O delineamento experimental foi o de blocos ao acaso com três repetições. O ensaio foi composto por 14 genótipos, sendo 12 linhagens e duas cultivares comerciais utilizadas como testemunha. Dez ensaios foram realizados, sendo cinco no Rio Grande do Sul, quatro no Paraná e um em Santa Catarina. Com base nos dados apresentados, as linhagens PFC 2014172, PFC 2014176, PFC 2015014 e PFC 2015062 são as mais promissoras em função do comportamento agronômico e das características de qualidade dos grãos para uso na malteação. |
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Palavras-Chave: |
Qualidade para malteação; Rendimento de grãos. |
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Thesagro: |
Hordeum Vulgare. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1155791/1/Ensaios-VCU-3-do-programa-de-melhoramento-de-cevada-cervejeira-65-69.pdf
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Marc: |
LEADER 01292nam a2200181 a 4500
001 2155791
005 2023-08-09
008 2023 bl uuuu u00u1 u #d
100 1 $aVILARINHO, A. A.
245 $aEnsaios VCU 3 do programa de melhoramento de cevada cervejeira da Embrapa em 2019.$h[electronic resource]
260 $aIn: REUNIÃO NACIONAL DE PESQUISA DE CEVADA, 33., 2023, Passo Fundo. Anais... Passo Fundo: Acervus$c2023
300 $ap. 65-69.
520 $aResumo: O presente trabalho teve por objetivo avaliar os ensaios de valor de cultivo e uso (VCU 3) de cevada cervejeira da Embrapa, em 2019. O delineamento experimental foi o de blocos ao acaso com três repetições. O ensaio foi composto por 14 genótipos, sendo 12 linhagens e duas cultivares comerciais utilizadas como testemunha. Dez ensaios foram realizados, sendo cinco no Rio Grande do Sul, quatro no Paraná e um em Santa Catarina. Com base nos dados apresentados, as linhagens PFC 2014172, PFC 2014176, PFC 2015014 e PFC 2015062 são as mais promissoras em função do comportamento agronômico e das características de qualidade dos grãos para uso na malteação.
650 $aHordeum Vulgare
653 $aQualidade para malteação
653 $aRendimento de grãos
700 1 $aPIRES, J. L. F.
700 1 $aANTONIAZZI, N.
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