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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
24/12/2008 |
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Data da última atualização: |
15/01/2009 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
GUERRA, M.; MORAES, A. P.; MIRKOV, E.; BRASILEIRO-VIDAL, A. C.; SILVA, A. E. B. e; SOARES FILHO, W. dos S. |
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Afiliação: |
M. Guerra, UFPE; A. P. Moraes, UFPE; E. Mirkov, AgriLife Research; A. C. Brasileiro-Vidal, UFPE; A. E. Barros e Silva, UFPE; Walter dos Santos Soares Filho, CNPMF. |
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Título: |
Molecular characterization of chromosomes of Poncirus trifoliata using different DNA sequences. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan,China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 25. 19. |
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Idioma: |
Inglês |
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Conteúdo: |
In order to get a first molecular cytogenetic map of each chromosome of Poncirus trifoliata, we localized by fluorescent in situ hybridization (FISH) several different probes. Chromosomes were firstly stained with the fluorochromes CMA and DAPI which allow the identification of heterochromatin as CMA+/DAPI- bands, dividing the nine chromosome pairs into three groups: two chromosome pairs bearing a proximal and a terminal band (B type), four having only a single terminal band (D type) and three others without any band (F type). Sequential CMA/DAPI staining and FISH using the 5S and 45S rDNA probes showed that these two probes were always adjacent to each other. The 45S rDNA probe co-located with the proximal band of all B type chromosome and with the single band of one D chromosome. A satelite DNA sequence isolated from Citrus sinensis, using primers based on the 181 bp satelite sequence of C. ichangensis described by Beridze et al. (1992), hybridzed at all CMA+ bands except the rDNA sites and a single terminal band in a B chromosome, suggesting that this is the main satelite sequence of Citrus and Poncirus. Further, 24 BACs from a P. trifoliata genomic library were selected and hybridized in situ, including four BACs from the Ctv resistance gene region. Seven out of the 24 probes gave disperse signals, four did not give any signal and 13 produced single copy homozygous signals in seven of the nine chromosome pairs (two D pairs were not labeled). The four BACs from the Ctv region were located very close to each other at the smallest F chromosome type, precisely in the interstitial region of the more distended arm. MenosIn order to get a first molecular cytogenetic map of each chromosome of Poncirus trifoliata, we localized by fluorescent in situ hybridization (FISH) several different probes. Chromosomes were firstly stained with the fluorochromes CMA and DAPI which allow the identification of heterochromatin as CMA+/DAPI- bands, dividing the nine chromosome pairs into three groups: two chromosome pairs bearing a proximal and a terminal band (B type), four having only a single terminal band (D type) and three others without any band (F type). Sequential CMA/DAPI staining and FISH using the 5S and 45S rDNA probes showed that these two probes were always adjacent to each other. The 45S rDNA probe co-located with the proximal band of all B type chromosome and with the single band of one D chromosome. A satelite DNA sequence isolated from Citrus sinensis, using primers based on the 181 bp satelite sequence of C. ichangensis described by Beridze et al. (1992), hybridzed at all CMA+ bands except the rDNA sites and a single terminal band in a B chromosome, suggesting that this is the main satelite sequence of Citrus and Poncirus. Further, 24 BACs from a P. trifoliata genomic library were selected and hybridized in situ, including four BACs from the Ctv resistance gene region. Seven out of the 24 probes gave disperse signals, four did not give any signal and 13 produced single copy homozygous signals in seven of the nine chromosome pairs (two D pairs were not labeled). The four BACs from the Ctv... Mostrar Tudo |
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Palavras-Chave: |
Citros. |
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Thesagro: |
Biotecnologia. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02333naa a2200205 a 4500
001 1655685
005 2009-01-15
008 2008 bl uuuu u00u1 u #d
100 1 $aGUERRA, M.
245 $aMolecular characterization of chromosomes of Poncirus trifoliata using different DNA sequences.
260 $c2008
520 $aIn order to get a first molecular cytogenetic map of each chromosome of Poncirus trifoliata, we localized by fluorescent in situ hybridization (FISH) several different probes. Chromosomes were firstly stained with the fluorochromes CMA and DAPI which allow the identification of heterochromatin as CMA+/DAPI- bands, dividing the nine chromosome pairs into three groups: two chromosome pairs bearing a proximal and a terminal band (B type), four having only a single terminal band (D type) and three others without any band (F type). Sequential CMA/DAPI staining and FISH using the 5S and 45S rDNA probes showed that these two probes were always adjacent to each other. The 45S rDNA probe co-located with the proximal band of all B type chromosome and with the single band of one D chromosome. A satelite DNA sequence isolated from Citrus sinensis, using primers based on the 181 bp satelite sequence of C. ichangensis described by Beridze et al. (1992), hybridzed at all CMA+ bands except the rDNA sites and a single terminal band in a B chromosome, suggesting that this is the main satelite sequence of Citrus and Poncirus. Further, 24 BACs from a P. trifoliata genomic library were selected and hybridized in situ, including four BACs from the Ctv resistance gene region. Seven out of the 24 probes gave disperse signals, four did not give any signal and 13 produced single copy homozygous signals in seven of the nine chromosome pairs (two D pairs were not labeled). The four BACs from the Ctv region were located very close to each other at the smallest F chromosome type, precisely in the interstitial region of the more distended arm.
650 $aBiotecnologia
653 $aCitros
700 1 $aMORAES, A. P.
700 1 $aMIRKOV, E.
700 1 $aBRASILEIRO-VIDAL, A. C.
700 1 $aSILVA, A. E. B. e
700 1 $aSOARES FILHO, W. dos S.
773 $tIn: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan,China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 25. 19.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
26/02/2015 |
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Data da última atualização: |
07/04/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
MATALLO, M. B.; ALMEIDA, S. D. B.; FRANCO, D. A. S.; CERDEIRA, A. L.; GAZZIERO, D. L. P. |
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Afiliação: |
Instituto Biológico - Campinas; Instituto Biológico - Campinas; Instituto Biológico - Campinas; ANTONIO LUIZ CERDEIRA, CNPMA; DIONISIO LUIZ PISA GAZZIERO, CNPSO. |
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Título: |
Glyphosate as a toll to produce shikimic acid in plants. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Planta Daninha, Viçosa, MG, v. 32, n. 3, p. 601-608, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT - Oseltamivir phosphate is a potent viral inhibitor produced from shikimic acid extracted from seeds of Ilicium verum, the most important natural source. With the site of action 5-enolpyruvylshikimate-3-phosphate synthase (EPSP), glyphosate is the only compound capable of inhibiting its activity with the consequent accumulation of shikimic acid in plants. Corn and soybean plants were sprayed with reduced rates of glyphosate (0.0 to 230.4 g a.i. ha-1) and shikimic acid content in the dry mass was determined by HPLC 3, 7 and 10 days after application. Results showed shikimic acid accumulation in dry mass with increases of up to 969% in corn and 33,000% on soybeans, with peak concentrations 3 days after treatment (DAT). Industrial feasibility for shikimic acid production, combined with favorable climatic conditions for growing corn and soybean in virtually all over Brazil, favor the use of reduced rates of glyphosate in shikimic acid biosynthesis, with potential for use as an inducer in exploration of alternative sources for production of oseltamivir phosphate with low environmental impact. RESUMO - O fosfato de oseltamivir, ingrediente ativo do antiviral Tamiflu®, é um potente inibidor viral produzido a partir do ácido chiquímico e extraído da semente de Ilicium verum, sua mais importante fonte natural. Tendo como sítio de ação a enzima 5 enolpiruvilchiquimato-3-fosfato sintase (EPSPs), o glyphosate é o único composto capaz de inibir a sua atividade, com o consequente acúmulo do ácido chiquímico nas plantas. Plantas de milho e soja foram pulverizadas com subdoses de glyphosate (0,0 a 230,4 g e.a. ha-1), determinando-se o teor de ácido chiquímico na massa seca dessas plantas por HPLC aos 3,7 e 10 dias após aplicação. Os resultados mostraram acúmulo de ácido chiquímico na massa seca dessas plantas, com aumento de até 969% no milho e 33.000% na soja, com picos de concentração aos 3 DAT. A praticabilidade industrial do processo de obtenção do ácido chiquímico, aliada às condições edafoclimáticas favoráveis ao plantio de milho e soja em diversos países, favorece o uso de subdoses de glyphosate na biossíntese de ácido chiquímico, com potencial para ser explorado como indutor na produção do fosfato de oseltamivir com baixo impacto ambiental. MenosABSTRACT - Oseltamivir phosphate is a potent viral inhibitor produced from shikimic acid extracted from seeds of Ilicium verum, the most important natural source. With the site of action 5-enolpyruvylshikimate-3-phosphate synthase (EPSP), glyphosate is the only compound capable of inhibiting its activity with the consequent accumulation of shikimic acid in plants. Corn and soybean plants were sprayed with reduced rates of glyphosate (0.0 to 230.4 g a.i. ha-1) and shikimic acid content in the dry mass was determined by HPLC 3, 7 and 10 days after application. Results showed shikimic acid accumulation in dry mass with increases of up to 969% in corn and 33,000% on soybeans, with peak concentrations 3 days after treatment (DAT). Industrial feasibility for shikimic acid production, combined with favorable climatic conditions for growing corn and soybean in virtually all over Brazil, favor the use of reduced rates of glyphosate in shikimic acid biosynthesis, with potential for use as an inducer in exploration of alternative sources for production of oseltamivir phosphate with low environmental impact. RESUMO - O fosfato de oseltamivir, ingrediente ativo do antiviral Tamiflu®, é um potente inibidor viral produzido a partir do ácido chiquímico e extraído da semente de Ilicium verum, sua mais importante fonte natural. Tendo como sítio de ação a enzima 5 enolpiruvilchiquimato-3-fosfato sintase (EPSPs), o glyphosate é o único composto capaz de inibir a sua atividade, com o consequente... Mostrar Tudo |
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Palavras-Chave: |
Planta daninha. |
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Thesaurus NAL: |
Weeds. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/118856/1/Glyphosate-as-a-toll-to-produce-shikimic-acid-in-plants..pdf
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Marc: |
LEADER 02870naa a2200193 a 4500
001 2010065
005 2022-04-07
008 2014 bl uuuu u00u1 u #d
100 1 $aMATALLO, M. B.
245 $aGlyphosate as a toll to produce shikimic acid in plants.$h[electronic resource]
260 $c2014
520 $aABSTRACT - Oseltamivir phosphate is a potent viral inhibitor produced from shikimic acid extracted from seeds of Ilicium verum, the most important natural source. With the site of action 5-enolpyruvylshikimate-3-phosphate synthase (EPSP), glyphosate is the only compound capable of inhibiting its activity with the consequent accumulation of shikimic acid in plants. Corn and soybean plants were sprayed with reduced rates of glyphosate (0.0 to 230.4 g a.i. ha-1) and shikimic acid content in the dry mass was determined by HPLC 3, 7 and 10 days after application. Results showed shikimic acid accumulation in dry mass with increases of up to 969% in corn and 33,000% on soybeans, with peak concentrations 3 days after treatment (DAT). Industrial feasibility for shikimic acid production, combined with favorable climatic conditions for growing corn and soybean in virtually all over Brazil, favor the use of reduced rates of glyphosate in shikimic acid biosynthesis, with potential for use as an inducer in exploration of alternative sources for production of oseltamivir phosphate with low environmental impact. RESUMO - O fosfato de oseltamivir, ingrediente ativo do antiviral Tamiflu®, é um potente inibidor viral produzido a partir do ácido chiquímico e extraído da semente de Ilicium verum, sua mais importante fonte natural. Tendo como sítio de ação a enzima 5 enolpiruvilchiquimato-3-fosfato sintase (EPSPs), o glyphosate é o único composto capaz de inibir a sua atividade, com o consequente acúmulo do ácido chiquímico nas plantas. Plantas de milho e soja foram pulverizadas com subdoses de glyphosate (0,0 a 230,4 g e.a. ha-1), determinando-se o teor de ácido chiquímico na massa seca dessas plantas por HPLC aos 3,7 e 10 dias após aplicação. Os resultados mostraram acúmulo de ácido chiquímico na massa seca dessas plantas, com aumento de até 969% no milho e 33.000% na soja, com picos de concentração aos 3 DAT. A praticabilidade industrial do processo de obtenção do ácido chiquímico, aliada às condições edafoclimáticas favoráveis ao plantio de milho e soja em diversos países, favorece o uso de subdoses de glyphosate na biossíntese de ácido chiquímico, com potencial para ser explorado como indutor na produção do fosfato de oseltamivir com baixo impacto ambiental.
650 $aWeeds
653 $aPlanta daninha
700 1 $aALMEIDA, S. D. B.
700 1 $aFRANCO, D. A. S.
700 1 $aCERDEIRA, A. L.
700 1 $aGAZZIERO, D. L. P.
773 $tPlanta Daninha, Viçosa, MG$gv. 32, n. 3, p. 601-608, 2014.
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