Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
30/10/2017 |
Data da última atualização: |
31/10/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BEZERRA JUNIOR, R. Q.; ELOY, A. M. X.; FURTADO, J. R.; PINHEIRO, R. R.; ANDRIOLI, A.; MORENO, F. B.; LOBO, M. D. P.; MONTEIRO-MOREIRA, A. C. O.; MOREIRA, R. de A.; PINTO, T. M. F.; TEIXEIRA, M. F. da S. |
Afiliação: |
Rosivaldo Quirino Bezerra Júnior, Laboratory of Virology (LABOVIR), Ceará State University (UECE) - Fortaleza, Ceará, Brazil; ANGELA MARIA XAVIER ELOY, CNPC; JOAO RICARDO FURTADO, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; ALICE ANDRIOLI, CNPC; Frederico Bruno Moreno, Center of Experimental Biology (Nubex), University of Fortaleza (UNIFOR), Fortaleza, Ceará, Brazil; Marina Duarte Pinto Lobo, Center of Experimental Biology (Nubex), University of Fortaleza (UNIFOR), Fortaleza, Ceará, Brazil; Ana Cristina O. Monteiro-Moreira, Center of Experimental Biology (Nubex), University of Fortaleza (UNIFOR), Fortaleza, Ceará, Brazil; Renato de Azevedo Moreira; Tatiana Maria Farias Pinto, Molecular Biology Laboratory, Vale do Acaraú State University (UVA) - Sobral, Ceará, Brazil; Maria Fátima da Silva Teixeira, Laboratory of Virology (LABOVIR), Ceará State University (UECE) - Fortaleza, Ceará, Brazil. |
Título: |
A panel of protein candidates for comprehensive study of Caprine Arthritis Encephalitis (CAE) infection. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Tropical Animal Health and Production, v. 50, n. 1, n. 7, p. 43-48, Oct. 2018. |
DOI: |
10.1007/s11250-017-1398-1 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The caprine arthrite encephalite (CAE) is a disease that affects especially dairy goat. The virus shows compartmentalization features, that allows it to hide at certain times during the course of the disease, making it difficult to control. The present study was conducted to identify the major seminal plasma protein profile of goats infected by CAE and its associations with seroconversion using Western blotting. Two groups containing five males each, were used in this experiment. The first group was composed by seropositive animals and the control by seronegative confirmed by Western blotting and PCR. The semen was collected through artificial vagina and after that, two-dimensional electrophoresis and MALDI-TOF MS were used. Seventy-five spots were identified in the goat seminal plasma gels, equivalent to 13 different proteins with more expression. The similar proteins found in both groups and related to reproduction were spermadhesin Z13-like, bodhesin and bodhesin-2, Lipocalin, protein PDC-109-like, and albumin. In infected goats, proteases such as arisulfatase A have been identified, whose function probably is related to metabolism control of sulfatides, involved to virus control. The other ones were bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase, cathepsin F isoform X1, disintegrin and metalloproteinase domain-containing protein 2-like isoform X1, clusterin, carbonic anhydrase 2, electron transfer flavoprotein subunit beta, and epididymal secretory glutathione peroxidase. The results of this study show the reaction of the innate immune system against chronic infection of goats by CAE. MenosAbstract: The caprine arthrite encephalite (CAE) is a disease that affects especially dairy goat. The virus shows compartmentalization features, that allows it to hide at certain times during the course of the disease, making it difficult to control. The present study was conducted to identify the major seminal plasma protein profile of goats infected by CAE and its associations with seroconversion using Western blotting. Two groups containing five males each, were used in this experiment. The first group was composed by seropositive animals and the control by seronegative confirmed by Western blotting and PCR. The semen was collected through artificial vagina and after that, two-dimensional electrophoresis and MALDI-TOF MS were used. Seventy-five spots were identified in the goat seminal plasma gels, equivalent to 13 different proteins with more expression. The similar proteins found in both groups and related to reproduction were spermadhesin Z13-like, bodhesin and bodhesin-2, Lipocalin, protein PDC-109-like, and albumin. In infected goats, proteases such as arisulfatase A have been identified, whose function probably is related to metabolism control of sulfatides, involved to virus control. The other ones were bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase, cathepsin F isoform X1, disintegrin and metalloproteinase domain-containing protein 2-like isoform X1, clusterin, carbonic anhydrase 2, electron transfer flavoprotein subunit beta, and epididymal secr... Mostrar Tudo |
Palavras-Chave: |
Artrite Encefalite Caprina; Arylsulfatase A; Proteomic; Viroses; Virus disease. |
Thesagro: |
Caprino; Doença animal; Genética Molecular; Infecção; Marcador genético; Sêmen; Vírus. |
Thesaurus Nal: |
Biomarkers; Caprine arthritis encephalitis virus; Dairy goats; Genetic markers; Infectious diseases; Lentivirus; Proteomics; Viral proteins. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 03050naa a2200493 a 4500 001 2078384 005 2023-10-31 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1007/s11250-017-1398-1$2DOI 100 1 $aBEZERRA JUNIOR, R. Q. 245 $aA panel of protein candidates for comprehensive study of Caprine Arthritis Encephalitis (CAE) infection. 260 $c2018 520 $aAbstract: The caprine arthrite encephalite (CAE) is a disease that affects especially dairy goat. The virus shows compartmentalization features, that allows it to hide at certain times during the course of the disease, making it difficult to control. The present study was conducted to identify the major seminal plasma protein profile of goats infected by CAE and its associations with seroconversion using Western blotting. Two groups containing five males each, were used in this experiment. The first group was composed by seropositive animals and the control by seronegative confirmed by Western blotting and PCR. The semen was collected through artificial vagina and after that, two-dimensional electrophoresis and MALDI-TOF MS were used. Seventy-five spots were identified in the goat seminal plasma gels, equivalent to 13 different proteins with more expression. The similar proteins found in both groups and related to reproduction were spermadhesin Z13-like, bodhesin and bodhesin-2, Lipocalin, protein PDC-109-like, and albumin. In infected goats, proteases such as arisulfatase A have been identified, whose function probably is related to metabolism control of sulfatides, involved to virus control. The other ones were bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase, cathepsin F isoform X1, disintegrin and metalloproteinase domain-containing protein 2-like isoform X1, clusterin, carbonic anhydrase 2, electron transfer flavoprotein subunit beta, and epididymal secretory glutathione peroxidase. The results of this study show the reaction of the innate immune system against chronic infection of goats by CAE. 650 $aBiomarkers 650 $aCaprine arthritis encephalitis virus 650 $aDairy goats 650 $aGenetic markers 650 $aInfectious diseases 650 $aLentivirus 650 $aProteomics 650 $aViral proteins 650 $aCaprino 650 $aDoença animal 650 $aGenética Molecular 650 $aInfecção 650 $aMarcador genético 650 $aSêmen 650 $aVírus 653 $aArtrite Encefalite Caprina 653 $aArylsulfatase A 653 $aProteomic 653 $aViroses 653 $aVirus disease 700 1 $aELOY, A. M. X. 700 1 $aFURTADO, J. R. 700 1 $aPINHEIRO, R. R. 700 1 $aANDRIOLI, A. 700 1 $aMORENO, F. B. 700 1 $aLOBO, M. D. P. 700 1 $aMONTEIRO-MOREIRA, A. C. O. 700 1 $aMOREIRA, R. de A. 700 1 $aPINTO, T. M. F. 700 1 $aTEIXEIRA, M. F. da S. 773 $tTropical Animal Health and Production$gv. 50, n. 1, n. 7, p. 43-48, Oct. 2018.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|