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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
09/11/2017 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MONTEIRO, J. P.; SANTOS, J. M. L. dos; ANDRÉ, W. P. P.; VASCONCELOS, J. F.; FROTA, G. A.; RIBEIRO, W. L. C.; FREITAS, E. P. de; MACEDO, I. T. F.; TEIXEIRA, M.; VIEIRA, L. da S.; BEVILAQUA, C. M. L. |
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Afiliação: |
JOMAR PATRICIO MONTEIRO, CNPC; Jessica Maria Leite dos Santos, Pós-graduação - Universidade Estadual do Ceará (UECe) - Fortaleza, CE, Brazil; Weibson Paz Pinheiro Andre, UECe - Fortaleza, CE, Brazil; Janaelia Ferreira Vasconcelos, Universidade Estadual do Vale do Acaraú (UVA) - Sobral, CE, Brazil; Gracielle Araújo Frota, UVA - Sobral, CE, Brazil; Wesley Lyeverton Correia Ribeiro, UECe - Fortaleza, CE, Brazil; Edilson Pereira de Freitas; Iara Tersia Freitas Macedo; MARCEL TEIXEIRA, CNPC; LUIZ DA SILVA VIEIRA, CNPC; Claudia Maria Leal Bevilaqua, UECe - Fortaleza, CE, Brazil. |
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Título: |
Molecular diagnostic for levamisol resistance in Haemonchus contortus. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE OF THE WORLD ASSOCIATION FOR THE ADVANCEMENT OF VETERINARY PARASITOLOGY, 26., 2017, Kuala Lumpur, Malaysia. Abstract book. Perth: WAAVP, 2017. p. 381. Abstract 4592. |
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Idioma: |
Inglês |
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Notas: |
In conjunction with 53rd MSPTM Annual Conference. |
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Conteúdo: |
Abstract: In tropical areas, Haemonchus contortus is the leading cause of production losses in small ruminant herds and its control is traditionally done through the utilization of synthetic anthelmintics. Levamisole, an imidazothiazole derivative, is widely used in Brazil and the occurrence of resistance is not uncommon. The genetic base for levamisole resistance in H. contortus is still under investigation, but it has been recently associated with a 63 bp deletion in the Hco-acr-8 gene, which codes for one of the subunits of a levamisole-sensistive acetylcholine receptor. Here we describe a real time PCR test for the detection and quantification of the presence and absence of this deletion. Reactions contained 12.5ul 2 × Fast Start Universal SYBR Green Master Mix (Roche, West Sussex, UK), 0.3 pmol/ul of each primer (forward and reverse), 50 ng of DNA and water for a total volume of 25?l. Amplification conditions for were: 95 oC for 10 min and 35 cycles at 95 oC for 15 s and at 56 ºC for 30 s. We tested the assay in two known H. contortus isolates, one resistant (Kokstad isolate - KOK) and another susceptible (Inbred-susceptible-Edinburgh - ISE). We also used the test to characterize a local H. contortus population previously exposed to levamisole. Preliminay results are in agreement with previously reported data as only the resistant allele was detected in the KOK isolate and both alleles were detected in the ISE isolate suggesting that this test may be useful in the fast detection of levamisole resistance in H. contortus. MenosAbstract: In tropical areas, Haemonchus contortus is the leading cause of production losses in small ruminant herds and its control is traditionally done through the utilization of synthetic anthelmintics. Levamisole, an imidazothiazole derivative, is widely used in Brazil and the occurrence of resistance is not uncommon. The genetic base for levamisole resistance in H. contortus is still under investigation, but it has been recently associated with a 63 bp deletion in the Hco-acr-8 gene, which codes for one of the subunits of a levamisole-sensistive acetylcholine receptor. Here we describe a real time PCR test for the detection and quantification of the presence and absence of this deletion. Reactions contained 12.5ul 2 × Fast Start Universal SYBR Green Master Mix (Roche, West Sussex, UK), 0.3 pmol/ul of each primer (forward and reverse), 50 ng of DNA and water for a total volume of 25?l. Amplification conditions for were: 95 oC for 10 min and 35 cycles at 95 oC for 15 s and at 56 ºC for 30 s. We tested the assay in two known H. contortus isolates, one resistant (Kokstad isolate - KOK) and another susceptible (Inbred-susceptible-Edinburgh - ISE). We also used the test to characterize a local H. contortus population previously exposed to levamisole. Preliminay results are in agreement with previously reported data as only the resistant allele was detected in the KOK isolate and both alleles were detected in the ISE isolate suggesting that this test may be useful in the fast ... Mostrar Tudo |
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Palavras-Chave: |
Resistance to anthelmintics. |
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Thesagro: |
Anti-Helmíntico; Caprino; Haemonchus Contortus; Ovino; Resistência a produtos químicos. |
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Thesaurus Nal: |
Anthelmintics; Brazil; Drug resistance; Goats; Levamisole; Sheep; Small ruminants. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166470/1/cnpc-2017-Molecular-diagnostic.pdf
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Marc: |
LEADER 02847nam a2200397 a 4500
001 2079274
005 2019-09-23
008 2017 bl uuuu u00u1 u #d
100 1 $aMONTEIRO, J. P.
245 $aMolecular diagnostic for levamisol resistance in Haemonchus contortus.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE OF THE WORLD ASSOCIATION FOR THE ADVANCEMENT OF VETERINARY PARASITOLOGY, 26., 2017, Kuala Lumpur, Malaysia. Abstract book. Perth: WAAVP, 2017. p. 381. Abstract 4592.$c4592
500 $aIn conjunction with 53rd MSPTM Annual Conference.
520 $aAbstract: In tropical areas, Haemonchus contortus is the leading cause of production losses in small ruminant herds and its control is traditionally done through the utilization of synthetic anthelmintics. Levamisole, an imidazothiazole derivative, is widely used in Brazil and the occurrence of resistance is not uncommon. The genetic base for levamisole resistance in H. contortus is still under investigation, but it has been recently associated with a 63 bp deletion in the Hco-acr-8 gene, which codes for one of the subunits of a levamisole-sensistive acetylcholine receptor. Here we describe a real time PCR test for the detection and quantification of the presence and absence of this deletion. Reactions contained 12.5ul 2 × Fast Start Universal SYBR Green Master Mix (Roche, West Sussex, UK), 0.3 pmol/ul of each primer (forward and reverse), 50 ng of DNA and water for a total volume of 25?l. Amplification conditions for were: 95 oC for 10 min and 35 cycles at 95 oC for 15 s and at 56 ºC for 30 s. We tested the assay in two known H. contortus isolates, one resistant (Kokstad isolate - KOK) and another susceptible (Inbred-susceptible-Edinburgh - ISE). We also used the test to characterize a local H. contortus population previously exposed to levamisole. Preliminay results are in agreement with previously reported data as only the resistant allele was detected in the KOK isolate and both alleles were detected in the ISE isolate suggesting that this test may be useful in the fast detection of levamisole resistance in H. contortus.
650 $aAnthelmintics
650 $aBrazil
650 $aDrug resistance
650 $aGoats
650 $aLevamisole
650 $aSheep
650 $aSmall ruminants
650 $aAnti-Helmíntico
650 $aCaprino
650 $aHaemonchus Contortus
650 $aOvino
650 $aResistência a produtos químicos
653 $aResistance to anthelmintics
700 1 $aSANTOS, J. M. L. dos
700 1 $aANDRÉ, W. P. P.
700 1 $aVASCONCELOS, J. F.
700 1 $aFROTA, G. A.
700 1 $aRIBEIRO, W. L. C.
700 1 $aFREITAS, E. P. de
700 1 $aMACEDO, I. T. F.
700 1 $aTEIXEIRA, M.
700 1 $aVIEIRA, L. da S.
700 1 $aBEVILAQUA, C. M. L.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 5 | |
| 3. |  | FERNANDES, A. A. O.; MACHADO, F. H. F.; ANDRADE, J. M. de S.; MENEZES, F. A. B. de; CATUNDA, A. G.; FIGUEIREDO, E. A. P. de. Desempenho de caprinos nativos e ovinos morada nova em diferentes pastagens no Ceara. Pesquisa Agropecuaria Brasileira, Brasilia, v.22, n.9/10, p.1061-1066, set./out.1987.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Unidades Centrais. |
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| 4. | | FERNANDES, A. A. O.; MACHADO, F. H. F.; ANDRADE, J. M. de S.; FIGUEIREDO, E. A. P. de; SHELTON, M.; PANT, K. P. Efeito do cruzamento sobre o crescimento de caprinos no Ceara. Pesquisa Agropecuaria Brasileira, Brasilia, v.20, n.1, p.109-114, jan. 1985.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Unidades Centrais. |
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| 5. | | FERNANDES, A. A. O.; ANDRADE, J. M. de S.; MENEZES, F. A. B. de; CATUNDA, A. G.; FIGUEIREDO, E. A. P. de. Recria de caprinos nativos e ovinos "morada nova" em pastagem de capim-buffel. Pesquisa Agropecuaria Brasileira, Brasilia, v.20, n.8, p.975-980, ago. 1985.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 5 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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