Data/hora: 08/06/2024 17:42:21

Biblioteca(s): 	Embrapa Agrobiologia.	
Data corrente: 	28/09/2022	
Data da última atualização: 	07/10/2022	
Autoria: 	AMARAL, M. B.; LOPES, T. dos S.; FEDER, C. B.; RIBEIRO, T. G.; PACHECO, R. S.; TEIXEIRA, T. N.; MONTEIRO, E. de C.; RAMALHO, I. O.; MACEDO, R. de O.; BODDEY, R. M.; ZILLI, J. E.; ALVES, B. J. R.	
Afiliação: 	MAYAN BLANC AMARAL, UFRRJ; TAMIRIS DOS SANTOS LOPES, UFRRJ; CAROLINE BUENO FEDER, UFRRJ; THIAGO GONÇALVES RIBEIRO, UFRRJ; RAFAEL SANCHES PACHECO, UFRRJ; TEIXEIRA THIAGO NEVES TEIXEIRA, UFRRJ; EDEVALDO DE CASTRO MONTEIRO, UFRRJ; ISRAEL OLIVEIRA RAMALHO, UFRRJ; ROBERT DE O. MACEDO, UFRRJ; ROBERT MICHAEL BODDEY, CNPAB; JERRI EDSON ZILLI, CNPAB; BRUNO JOSE RODRIGUES ALVES, CNPAB.	
Título: 	Bradyrhizobium occurrence in nodules of perennial horsegram.	
Ano de publicação: 	2022	
Fonte/Imprenta: 	Brazilian Journal of Microbiology Online, 17 set. 2022.	
ISSN: 	1678-4405	
DOI: 	10.1007/s42770-022-00821-2	
Idioma: 	Inglês	
Conteúdo: 	The introduction of a forage legume into a tropical pasture should decrease the need for N fertilizer, provided biological N-2 fixation (BNF) contributes enough to compensate for exported N. Macrotyloma axillare (perennial horsegram) is a suitable legume for composing mixed pastures, and our hypothesis is that the isolation of indigenous rhizobia from roots and rhizosphere is the way of achieving an efficient inoculant to maximize BNF to the legume. Nodules and rhizosphere soil taken from M. axillare grown in a mixed pasture with palisade grass were sampled and used in a trap host assay using Leonard jars containing a mixture of vermiculite and sand. A total of ten bacteria were initially isolated using this technique. The isolates were then used in two experiments to evaluate the inoculation responses on the perennial horsegram in greenhouse conditions to which nodulation, plant growth, and shoot N accumulation were measured. Phylogenetic analyses based on 16S rRNA and recA placed all strains within genus Bradyrhizobium, some of them not previously described. The best strain provided more than 120 nodules and more than 65 mg of nodules per plant. Strain BR14182 was considered the most promising given the high dry matter and N accumulation in plant shoots. This study provides the first analysis of Bradyrhizobium diversity nodulating M. axillare in Brazil and provided evidence of the role of inoculation in incrementing the plant-rhizobium symbiosis in a forage legume.	
Palavras-Chave: 	Inoculant; Macrotyloma axillare; Mixed pastures; Rhizobia.	
Categoria do assunto: 	S Ciências Biológicas	
Registro original: 	Embrapa Agrobiologia (CNPAB)	
Exemplares: 	CNPAB - (42019-1) - UPC - AP - DD -  -  -  - DIS