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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
24/08/2020 |
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Data da última atualização: |
25/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MORÁN DIEZ, M. E; TRANQUE, E.; BETTIOL, W.; MONTE, E.; HERMOSA, R. |
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Afiliação: |
MARIA EUGENIA MORÁN DIEZ, Universidad de Salamanca; EDUARDO TRANQUE, Centro de Investigaciones Biológicas Margarita Salas; WAGNER BETTIOL, CNPMA; ENRIQUE MONTE, Universidad de Salamanca; ROSA HERMOSA, Universidad de Salamanca. |
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Título: |
Differential response of tomato plants to the application of three Trichoderma species when evaluating the control of Pseudomonas syringae populations. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Plants, v. 9, n. 5, article 626, 2020. |
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ISSN: |
2223-7747 |
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DOI: |
https://doi.org/10.3390/plants9050626 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Trichoderma species are well known biocontrol agents that are able to induce responses in the host plants against an array of abiotic and biotic stresses. Here, we investigate, when applied to tomato seeds, the potential of Trichoderma strains belonging to three different species, T. parareesei T6, T. asperellum T25, and T. harzianum T34, to control the fully pathogenic strain Pseudomonas syringae pv. tomato (Pst) DC3000, able to produce the coronatine (COR) toxin, and the COR-deficient strain Pst DC3118 in tomato plants, and the molecular mechanisms by which the plant can modulate its systemic defense. Four-week old tomato plants, seed-inoculated, or not, with a Trichoderma strain, were infected, or not, with a Pst strain, and the changes in the expression of nine marker genes representative of salicylic acid (SA) (ICS1 and PAL5) and jasmonic acid (JA) (TomLoxC) biosynthesis, SA- (PR1b1), JA- (PINII and MYC2) and JA/Ethylene (ET)-dependent (ERF-A2) defense pathways, as well as the abscisic acid (ABA)-responsive gene AREB2 and the respiratory burst oxidase gene LERBOH1, were analyzed at 72 hours post-inoculation (hpi) with the bacteria. The significant increase obtained for bacterial population sizes in the leaves, disease index, and the upregulation of tomato genes related to SA, JA, ET and ABA in plants inoculated with Pst DC3000 compared with those obtained with Pst DC3118, confirmed the COR role as a virulence factor, and showed that both Pst and COR synergistically activate the JA- and SA-signaling defense responses, at least at 72 hpi. The three Trichoderma strains tested reduced the DC3118 levels to different extents and were able to control disease symptoms at the same rate. However, a minor protection (9.4%) against DC3000 was only achieved with T. asperellum T25. The gene deregulation detected in Trichoderma-treated plus Pst-inoculated tomato plants illustrates the complex system of a phytohormone-mediated signaling network that is affected by the pathogen and Trichoderma applications but also by their interaction. The expression changes for all nine genes analyzed, excepting LERBOH1, as well as the bacterial populations in the leaves were significantly affected by the interaction. Our results show that Trichoderma spp. are not adequate to control the disease caused by fully pathogenic Pst strains in tomato plants. MenosAbstract: Trichoderma species are well known biocontrol agents that are able to induce responses in the host plants against an array of abiotic and biotic stresses. Here, we investigate, when applied to tomato seeds, the potential of Trichoderma strains belonging to three different species, T. parareesei T6, T. asperellum T25, and T. harzianum T34, to control the fully pathogenic strain Pseudomonas syringae pv. tomato (Pst) DC3000, able to produce the coronatine (COR) toxin, and the COR-deficient strain Pst DC3118 in tomato plants, and the molecular mechanisms by which the plant can modulate its systemic defense. Four-week old tomato plants, seed-inoculated, or not, with a Trichoderma strain, were infected, or not, with a Pst strain, and the changes in the expression of nine marker genes representative of salicylic acid (SA) (ICS1 and PAL5) and jasmonic acid (JA) (TomLoxC) biosynthesis, SA- (PR1b1), JA- (PINII and MYC2) and JA/Ethylene (ET)-dependent (ERF-A2) defense pathways, as well as the abscisic acid (ABA)-responsive gene AREB2 and the respiratory burst oxidase gene LERBOH1, were analyzed at 72 hours post-inoculation (hpi) with the bacteria. The significant increase obtained for bacterial population sizes in the leaves, disease index, and the upregulation of tomato genes related to SA, JA, ET and ABA in plants inoculated with Pst DC3000 compared with those obtained with Pst DC3118, confirmed the COR role as a virulence factor, and showed that both Pst and COR synergisti... Mostrar Tudo |
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Palavras-Chave: |
(hemi)biotrophic bacteria; Systemic defense; Trichoderma parareesei. |
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Thesagro: |
Bactéria Patogênica; Fungo Para Controle Biológico; Pseudomonas Syringae; Tomate; Trichoderma; Trichoderma Harzianum. |
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Thesaurus Nal: |
Bacterial diseases of plants; Biological control agents; Defense mechanisms; Tomatoes; Trichoderma asperellum. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/215555/1/Bettiol-differential-response-2020.pdf
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Marc: |
LEADER 03522naa a2200361 a 4500
001 2124536
005 2021-08-25
008 2020 bl uuuu u00u1 u #d
022 $a2223-7747
024 7 $ahttps://doi.org/10.3390/plants9050626$2DOI
100 1 $aMORÁN DIEZ, M. E
245 $aDifferential response of tomato plants to the application of three Trichoderma species when evaluating the control of Pseudomonas syringae populations.$h[electronic resource]
260 $c2020
520 $aAbstract: Trichoderma species are well known biocontrol agents that are able to induce responses in the host plants against an array of abiotic and biotic stresses. Here, we investigate, when applied to tomato seeds, the potential of Trichoderma strains belonging to three different species, T. parareesei T6, T. asperellum T25, and T. harzianum T34, to control the fully pathogenic strain Pseudomonas syringae pv. tomato (Pst) DC3000, able to produce the coronatine (COR) toxin, and the COR-deficient strain Pst DC3118 in tomato plants, and the molecular mechanisms by which the plant can modulate its systemic defense. Four-week old tomato plants, seed-inoculated, or not, with a Trichoderma strain, were infected, or not, with a Pst strain, and the changes in the expression of nine marker genes representative of salicylic acid (SA) (ICS1 and PAL5) and jasmonic acid (JA) (TomLoxC) biosynthesis, SA- (PR1b1), JA- (PINII and MYC2) and JA/Ethylene (ET)-dependent (ERF-A2) defense pathways, as well as the abscisic acid (ABA)-responsive gene AREB2 and the respiratory burst oxidase gene LERBOH1, were analyzed at 72 hours post-inoculation (hpi) with the bacteria. The significant increase obtained for bacterial population sizes in the leaves, disease index, and the upregulation of tomato genes related to SA, JA, ET and ABA in plants inoculated with Pst DC3000 compared with those obtained with Pst DC3118, confirmed the COR role as a virulence factor, and showed that both Pst and COR synergistically activate the JA- and SA-signaling defense responses, at least at 72 hpi. The three Trichoderma strains tested reduced the DC3118 levels to different extents and were able to control disease symptoms at the same rate. However, a minor protection (9.4%) against DC3000 was only achieved with T. asperellum T25. The gene deregulation detected in Trichoderma-treated plus Pst-inoculated tomato plants illustrates the complex system of a phytohormone-mediated signaling network that is affected by the pathogen and Trichoderma applications but also by their interaction. The expression changes for all nine genes analyzed, excepting LERBOH1, as well as the bacterial populations in the leaves were significantly affected by the interaction. Our results show that Trichoderma spp. are not adequate to control the disease caused by fully pathogenic Pst strains in tomato plants.
650 $aBacterial diseases of plants
650 $aBiological control agents
650 $aDefense mechanisms
650 $aTomatoes
650 $aTrichoderma asperellum
650 $aBactéria Patogênica
650 $aFungo Para Controle Biológico
650 $aPseudomonas Syringae
650 $aTomate
650 $aTrichoderma
650 $aTrichoderma Harzianum
653 $a(hemi)biotrophic bacteria
653 $aSystemic defense
653 $aTrichoderma parareesei
700 1 $aTRANQUE, E.
700 1 $aBETTIOL, W.
700 1 $aMONTE, E.
700 1 $aHERMOSA, R.
773 $tPlants$gv. 9, n. 5, article 626, 2020.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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| Registros recuperados : 8 | |
| 5. |  | PÉREZ, E.; RUBIO, M. B.; CARDOZA, R. E.; GUTIÉRREZ, S.; BETTIOL, W.; MONTE, E.; HERMOSA, R. The importance of chorismate mutase in the biocontrol potential of Trichoderma parareesei. Frontiers in Microbiology, Lausanne, v. 6, 2015. Article 1181. 14 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 6. | | RUBIO, M. B.; VICENTE, R.; BETTIOL, W.; MORCUENDE, R.; MONTE, E.; HERMOSA, R. Tomato plant responses to salt stress after combined application of Trichoderma harzianum and chemical fertilization. In: INTERNATIONAL TRICHODERMA & GLIOCADIUM WORKSHOP, 15., 2018, Salamanca. Opportunism and conversations in the environment: proceedings. Salamanca: University of Salamanca, 2018. p. 67.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 7. | | RUBIO, M. B.; HERMOSA, R.; VICENTE, R.; GOMÉZ-ACOSTA, F. E.; MORCUENDE, R.; MONTE, E.; BETTIOL, W. The combination of trichoderma harzianum and chemical fertilization leads to the deregulation of phytohormone networking, preventing the adaptive responses of tomato plants to salt stress. Frontiers in Plant Science, v. 8, p. 1-14, 2017. Article 294.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 8. | | DOMINGUEZ, S.; RUBIO, M. B.; CARDOZA, R. E.; GUTIÉRREZ, S.; NICOLÁS, C.; BETTIOL, W.; HERMOSA, R.; MONTE, E. Nitrogen metabolism and growth enhancement in tomato plants challenged with Trichoderma harzianum expressing the Aspergillus nidulans acetamidase amdS gene. Frontiers in Microbiology, v. 7, article 1182, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 8 | |
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