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Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
04/01/2016 |
Data da última atualização: |
25/01/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PÉREZ, E.; RUBIO, M. B.; CARDOZA, R. E.; GUTIÉRREZ, S.; BETTIOL, W.; MONTE, E.; HERMOSA, R. |
Afiliação: |
ESCLAUDYS PEREZ, Universidad de Salamanca; MARIA BELEN RUBIO, Universidad de Salamanca; ROSA ELENA CARDOSA, Universidad de León; SANTIAGO GUTIERREZ, Universidad de León; WAGNER BETTIOL, CNPMA; ENRIQUE MONTE, Universidad de Salamanca; ROSA HERMOSA, Universidad de Salamanca. |
Título: |
The importance of chorismate mutase in the biocontrol potential of Trichoderma parareesei. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Frontiers in Microbiology, Lausanne, v. 6, 2015. Article 1181. |
Páginas: |
14 p. |
ISBN: |
http://dx.doi.org/10.3389/fmicb.2015.01181 |
Idioma: |
Inglês |
Conteúdo: |
Species of Trichoderma exert direct biocontrol activity against soil-borne plant pathogens due to their ability to compete for nutrients and to inhibit or kill their targets through the production of antibiotics and/or hydrolytic enzymes. In addition to these abilities, Trichoderma spp. have beneficial effects for plants, including the stimulation of defenses and the promotion of growth. Here we study the role in biocontrol of the T. parareesei Tparo7 gene, encoding a chorismate mutase (CM), a shikimate pathway branch point leading to the production of aromatic amino acids, which are not only essential components of protein synthesis but also the precursors of a wide range of secondary metabolites. We isolated T. parareesei transformants with the Tparo7 gene silenced. Compared with the wild-type, decreased levels of Tparo7 expression in the silenced transformants were accompanied by reduced CM activity, lower growth rates on different culture media, and reduced mycoparasitic behavior against the phytopathogenic fungi Rhizoctonia solani, Fusarium oxysporum and Botrytis cinerea in dual cultures. By contrast, higher amounts of the aromatic metabolites tyrosol, 2-phenylethanol and salicylic acid were detected in supernatants from the silenced transformants, which were able to inhibit the growth of F. oxysporum and B. cinerea. In in vitro plant assays, Tparo7-silenced transformants also showed a reduced capacity to colonize tomato roots. The effect of Tparo7-silencing on tomato plant responses was examined in greenhouse assays. The growth of plants colonized by the silenced transformants was reduced and the plants exhibited an increased susceptibility to B. cinerea in comparison with the responses observed for control plants. In addition, the plants turned yellowish and were defective in jasmonic acid- and ethylene-regulated signaling pathways which was seen by expression analysis of lipoxygenase 1 (LOX1), ethylene-insensitive protein 2 (EIN2) and pathogenesis-related protein 1 (PR-1) genes. MenosSpecies of Trichoderma exert direct biocontrol activity against soil-borne plant pathogens due to their ability to compete for nutrients and to inhibit or kill their targets through the production of antibiotics and/or hydrolytic enzymes. In addition to these abilities, Trichoderma spp. have beneficial effects for plants, including the stimulation of defenses and the promotion of growth. Here we study the role in biocontrol of the T. parareesei Tparo7 gene, encoding a chorismate mutase (CM), a shikimate pathway branch point leading to the production of aromatic amino acids, which are not only essential components of protein synthesis but also the precursors of a wide range of secondary metabolites. We isolated T. parareesei transformants with the Tparo7 gene silenced. Compared with the wild-type, decreased levels of Tparo7 expression in the silenced transformants were accompanied by reduced CM activity, lower growth rates on different culture media, and reduced mycoparasitic behavior against the phytopathogenic fungi Rhizoctonia solani, Fusarium oxysporum and Botrytis cinerea in dual cultures. By contrast, higher amounts of the aromatic metabolites tyrosol, 2-phenylethanol and salicylic acid were detected in supernatants from the silenced transformants, which were able to inhibit the growth of F. oxysporum and B. cinerea. In in vitro plant assays, Tparo7-silenced transformants also showed a reduced capacity to colonize tomato roots. The effect of Tparo7-silencing on tomato p... Mostrar Tudo |
Palavras-Chave: |
2-phenylethanol; Tparo7 gene; Tyrosol. |
Thesagro: |
Controle biológico; Trichoderma. |
Thesaurus Nal: |
Antifungal agents; Gene silencing; Phenylethyl alcohol; Salicylic acid; Shikimate pathway. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/136478/1/2015AP28.pdf
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Marc: |
LEADER 02926naa a2200325 a 4500 001 2032746 005 2016-01-25 008 2015 bl uuuu u00u1 u #d 100 1 $aPÉREZ, E. 245 $aThe importance of chorismate mutase in the biocontrol potential of Trichoderma parareesei.$h[electronic resource] 260 $c2015 300 $a14 p. 520 $aSpecies of Trichoderma exert direct biocontrol activity against soil-borne plant pathogens due to their ability to compete for nutrients and to inhibit or kill their targets through the production of antibiotics and/or hydrolytic enzymes. In addition to these abilities, Trichoderma spp. have beneficial effects for plants, including the stimulation of defenses and the promotion of growth. Here we study the role in biocontrol of the T. parareesei Tparo7 gene, encoding a chorismate mutase (CM), a shikimate pathway branch point leading to the production of aromatic amino acids, which are not only essential components of protein synthesis but also the precursors of a wide range of secondary metabolites. We isolated T. parareesei transformants with the Tparo7 gene silenced. Compared with the wild-type, decreased levels of Tparo7 expression in the silenced transformants were accompanied by reduced CM activity, lower growth rates on different culture media, and reduced mycoparasitic behavior against the phytopathogenic fungi Rhizoctonia solani, Fusarium oxysporum and Botrytis cinerea in dual cultures. By contrast, higher amounts of the aromatic metabolites tyrosol, 2-phenylethanol and salicylic acid were detected in supernatants from the silenced transformants, which were able to inhibit the growth of F. oxysporum and B. cinerea. In in vitro plant assays, Tparo7-silenced transformants also showed a reduced capacity to colonize tomato roots. The effect of Tparo7-silencing on tomato plant responses was examined in greenhouse assays. The growth of plants colonized by the silenced transformants was reduced and the plants exhibited an increased susceptibility to B. cinerea in comparison with the responses observed for control plants. In addition, the plants turned yellowish and were defective in jasmonic acid- and ethylene-regulated signaling pathways which was seen by expression analysis of lipoxygenase 1 (LOX1), ethylene-insensitive protein 2 (EIN2) and pathogenesis-related protein 1 (PR-1) genes. 650 $aAntifungal agents 650 $aGene silencing 650 $aPhenylethyl alcohol 650 $aSalicylic acid 650 $aShikimate pathway 650 $aControle biológico 650 $aTrichoderma 653 $a2-phenylethanol 653 $aTparo7 gene 653 $aTyrosol 700 1 $aRUBIO, M. B. 700 1 $aCARDOZA, R. E. 700 1 $aGUTIÉRREZ, S. 700 1 $aBETTIOL, W. 700 1 $aMONTE, E. 700 1 $aHERMOSA, R. 773 $tFrontiers in Microbiology, Lausanne$gv. 6, 2015. Article 1181.
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Embrapa Meio Ambiente (CNPMA) |
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Biblioteca(s): |
Embrapa Meio-Norte. |
Data corrente: |
02/10/2002 |
Data da última atualização: |
10/06/2011 |
Tipo da produção científica: |
Documentos |
Autoria: |
CARVALHO, J. H. de; MONTEIRO, F. das C.; GIRÃO, R. N. |
Afiliação: |
JOSÉ HERCULANO DE CARVALHO, CPAMN; FRANCISCO DAS CHAGAS MONTEIRO, CPAMN; RAIMUNDO NONATO GIRÃO, CPAMN. |
Título: |
Conservação do bovino pé-duro ou curraleiro: situação atual. |
Ano de publicação: |
2001 |
Fonte/Imprenta: |
Teresina: Embrapa Meio-Nortel, 2001. |
Páginas: |
16 p. |
Série: |
(Embrapa Meio-Norte. Documentos, 58). |
ISSN: |
0104-866X |
Idioma: |
Português |
Conteúdo: |
O gado pé-duro ou curraleiro é descendente dos bovinos trazidos pelos colonizadores portugueses e constitui um importante recurso genético para a pecuária brasileira, especialmente a nordestina. É uma raça muito resistente e bem adaptada a ambientes adversos, como a zona semi-árida do Nordeste. Entretanto, com a introdução de novas raças bovinos no Brasil, mais produtivas em ambientes melhorados, o gado pé-duro perdeu terreno e quase foi extinto. Este boletim traça um breve histório da raça e da s medidas que vêm sendo tomadas para sua conservação. E ressalta a atuação da Embrapa Meio-Norte, que tem particpação fundamental na sua conservação, em pareceria com a Embrapa Recursos Genéticos e Biotecnologia. |
Palavras-Chave: |
Animal genetic advance; Gado curraleiro; Gado pé-duro; Melhoramento genético; Native race; Raça nativa. |
Thesagro: |
Bovinocultura; Conservação; Melhoramento Animal; Melhoramento Genético Animal. |
Thesaurus NAL: |
cattle. |
Categoria do assunto: |
-- L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/35887/1/Doc58.pdf
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Marc: |
LEADER 01575nam a2200301 a 4500 001 1054346 005 2011-06-10 008 2001 bl uuuu u0uu1 u #d 022 $a0104-866X 100 1 $aCARVALHO, J. H. de 245 $aConservação do bovino pé-duro ou curraleiro$bsituação atual. 260 $aTeresina: Embrapa Meio-Nortel$c2001 300 $a16 p. 490 $a(Embrapa Meio-Norte. Documentos, 58). 520 $aO gado pé-duro ou curraleiro é descendente dos bovinos trazidos pelos colonizadores portugueses e constitui um importante recurso genético para a pecuária brasileira, especialmente a nordestina. É uma raça muito resistente e bem adaptada a ambientes adversos, como a zona semi-árida do Nordeste. Entretanto, com a introdução de novas raças bovinos no Brasil, mais produtivas em ambientes melhorados, o gado pé-duro perdeu terreno e quase foi extinto. Este boletim traça um breve histório da raça e da s medidas que vêm sendo tomadas para sua conservação. E ressalta a atuação da Embrapa Meio-Norte, que tem particpação fundamental na sua conservação, em pareceria com a Embrapa Recursos Genéticos e Biotecnologia. 650 $acattle 650 $aBovinocultura 650 $aConservação 650 $aMelhoramento Animal 650 $aMelhoramento Genético Animal 653 $aAnimal genetic advance 653 $aGado curraleiro 653 $aGado pé-duro 653 $aMelhoramento genético 653 $aNative race 653 $aRaça nativa 700 1 $aMONTEIRO, F. das C. 700 1 $aGIRÃO, R. N.
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