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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
19/04/2005 |
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Data da última atualização: |
30/05/2005 |
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Autoria: |
MONNERAT, R. G.; DIAS, D. G. S.; SILVA, S. F. da; MARTINS, E. S.; BERRY, C.; FALCÃO, R.; GOMES, A. C. M. M.; PRAÇA, L. B.; SOARES, C. M. S. |
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Título: |
Screening of Bacillus thuringiensis strains effective against mosquitoes. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 40, n. 2, p. 103-106, 2005. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this work was to evaluate 210 Bacillus thuringiensis strains against Aedes aegypti and Culex quinquefasciatus larvae to select the most effective. These strains were isolated from different regions of Brazil and are stored in a Bacillus spp. collection at Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil. The selected strains were characterized by morphological (microscopy), biochemical (SDS-PAGE 10%) and molecular (PCR) methods. Six B. thuringiensis strains were identified as mosquito-toxic after the selective bioassays. None of the strains produced the expected PCR products for detection of cry4, cry11 and cyt1A genes. These results indicate that the activity of mosquitocidal Brazilian strains are not related with Cry4, Cry11 or Cyt proteins, so they could be used as an alternative bioinsecticide against mosquitoes.
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Palavras-Chave: |
Aedes aegypt; Bioinseticida. |
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Thesagro: |
Bacillus Thuringiensis; Culex Quinquefasciatus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01621naa a2200265 a 4500
001 1185589
005 2005-05-30
008 2005 bl uuuu u00u1 u #d
100 1 $aMONNERAT, R. G.
245 $aScreening of Bacillus thuringiensis strains effective against mosquitoes.
260 $c2005
520 $aThe objective of this work was to evaluate 210 Bacillus thuringiensis strains against Aedes aegypti and Culex quinquefasciatus larvae to select the most effective. These strains were isolated from different regions of Brazil and are stored in a Bacillus spp. collection at Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil. The selected strains were characterized by morphological (microscopy), biochemical (SDS-PAGE 10%) and molecular (PCR) methods. Six B. thuringiensis strains were identified as mosquito-toxic after the selective bioassays. None of the strains produced the expected PCR products for detection of cry4, cry11 and cyt1A genes. These results indicate that the activity of mosquitocidal Brazilian strains are not related with Cry4, Cry11 or Cyt proteins, so they could be used as an alternative bioinsecticide against mosquitoes.
650 $aBacillus Thuringiensis
650 $aCulex Quinquefasciatus
653 $aAedes aegypt
653 $aBioinseticida
700 1 $aDIAS, D. G. S.
700 1 $aSILVA, S. F. da
700 1 $aMARTINS, E. S.
700 1 $aBERRY, C.
700 1 $aFALCÃO, R.
700 1 $aGOMES, A. C. M. M.
700 1 $aPRAÇA, L. B.
700 1 $aSOARES, C. M. S.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 40, n. 2, p. 103-106, 2005.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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