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Registro Completo |
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Biblioteca(s): |
Embrapa Acre; Embrapa Amazônia Ocidental. |
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Data corrente: |
02/06/2014 |
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Data da última atualização: |
06/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MIDORIKAWA, G. E. O.; SOUZA, M. de L. M. de; SILVA, O. F.; DIAS, J. do S. A.; KANZAKI, L. I. B.; HANADA, R. E.; MESQUITA, R. M. L. C.; GONCALVES, R. C.; ALVARES, V. de S.; BITTENCOURT, D. M. de C.; MILLER, R. N. G. |
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Afiliação: |
Glaucia E. O. Midorikawa, Universidade de Brasília (UnB); MARIA DE LOURDES MENDES DE SOUZA, CTAA; OTNIEL FREITAS SILVA, CTAA; JUREMA DO SOCORRO AZEVEDO DIAS, CPAF-AP; Luis I. B. Kanzaki, Universidade de Brasília (UnB); Rogerio E. Hanada, Instituto Nacional de Pesquisas da Amazônia (Inpa); Renata M. L. C. Mesquita, Instituto Nacional de Pesquisas da Amazônia (Inpa); RIVADALVE COELHO GONCALVES, CPAF-AC; VIRGINIA DE SOUZA ALVARES, CPAF-Acre; DANIELA MATIAS DE C BITTENCOURT, CPAA; Robert N. G. Miller, Universidade de Brasília (UnB). |
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Título: |
Characterization of Aspergillus species on Brazil nut from the Brazilian Amazonian region and development of a PCR assay for identification at the genus level. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
BMC Microbiology, v.14, n. 138, 2014. |
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ISSN: |
1471-2180 |
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DOI: |
10.1186/1471-2180-14-138 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five species, with Aspergillus section Flavi species A. nomius and A. flavus the most abundant. PCR primers ASP_GEN_MTSSU_F1 and ASP_GEN_MTSSU_R1 were designed for the genus Aspergillus, targeting a portion of the mitochondrial small subunit ribosomal RNA gene. Primer specificity was validated through both electronic PCR against target gene sequences at Genbank and in PCR reactions against DNA from Aspergillus species and other fungal genera common on Brazil nut. Collective differentiation of the observed section Flavi species A. flavus, A. nomius and A. tamarii from other Aspergillus species was possible on the basis of RFLP polymorphism. Conclusions:Given the abundance of Aspergillus section Flavi species A. nomius and A. flavus observed on Brazil nut, and associated risk of mycotoxin accumulation, simple identification methods for such mycotoxigenic species are of importance for Hazard Analysis Critical Control Point system implementation. The assay for the genus Aspergillus represents progress towards specific PCR identification and detection of mycotoxigenic species. MenosBackground: Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five species, with Aspergillus section Flavi species A. nomius and A. flavus the most abundant. PCR primers ASP_GEN_MTSSU_F1 and ASP_GEN_MTSSU_R1 were designed for the genus Aspergillus, targeting a portion of the mitochondrial small subunit ribosomal RNA gene. Primer specificity was validated through both electronic PCR against target gene sequences at Genbank and in PCR reactions against DNA from Aspergillus species and other fungal genera common on Brazil nut. Collective differentiation of the observed section Flavi species A. flavus, A. nomius and A. tamarii from other Aspergillus species was possible on the basis of RFLP polymorphism. Conclusions:Given the abundance of Aspergillus section Flavi species A. nomius ... Mostrar Tudo |
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Palavras-Chave: |
Castanha do brasil; Castanha-do-brasil; Enfermedades y desórdenes de las plantas; Identificación de patógenos; Nuez del Brasil; Pragas e doenças. |
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Thesagro: |
Aflatoxina; Aspergillus flavus; Bertholletia excelsa; Castanha do pará; Doença de planta; Fungo; Identificação. |
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Thesaurus Nal: |
Amazonia; Aspergillus; Brazil nuts; Fungi; Pathogen identification; Plant diseases and disorders. |
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Categoria do assunto: |
--
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/102994/1/castanha-Robert.pdf
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Marc: |
LEADER 03376naa a2200493 a 4500
001 1987473
005 2021-07-06
008 2014 bl uuuu u00u1 u #d
022 $a1471-2180
024 7 $a10.1186/1471-2180-14-138$2DOI
100 1 $aMIDORIKAWA, G. E. O.
245 $aCharacterization of Aspergillus species on Brazil nut from the Brazilian Amazonian region and development of a PCR assay for identification at the genus level.$h[electronic resource]
260 $c2014
520 $aBackground: Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five species, with Aspergillus section Flavi species A. nomius and A. flavus the most abundant. PCR primers ASP_GEN_MTSSU_F1 and ASP_GEN_MTSSU_R1 were designed for the genus Aspergillus, targeting a portion of the mitochondrial small subunit ribosomal RNA gene. Primer specificity was validated through both electronic PCR against target gene sequences at Genbank and in PCR reactions against DNA from Aspergillus species and other fungal genera common on Brazil nut. Collective differentiation of the observed section Flavi species A. flavus, A. nomius and A. tamarii from other Aspergillus species was possible on the basis of RFLP polymorphism. Conclusions:Given the abundance of Aspergillus section Flavi species A. nomius and A. flavus observed on Brazil nut, and associated risk of mycotoxin accumulation, simple identification methods for such mycotoxigenic species are of importance for Hazard Analysis Critical Control Point system implementation. The assay for the genus Aspergillus represents progress towards specific PCR identification and detection of mycotoxigenic species.
650 $aAmazonia
650 $aAspergillus
650 $aBrazil nuts
650 $aFungi
650 $aPathogen identification
650 $aPlant diseases and disorders
650 $aAflatoxina
650 $aAspergillus flavus
650 $aBertholletia excelsa
650 $aCastanha do pará
650 $aDoença de planta
650 $aFungo
650 $aIdentificação
653 $aCastanha do brasil
653 $aCastanha-do-brasil
653 $aEnfermedades y desórdenes de las plantas
653 $aIdentificación de patógenos
653 $aNuez del Brasil
653 $aPragas e doenças
700 1 $aSOUZA, M. de L. M. de
700 1 $aSILVA, O. F.
700 1 $aDIAS, J. do S. A.
700 1 $aKANZAKI, L. I. B.
700 1 $aHANADA, R. E.
700 1 $aMESQUITA, R. M. L. C.
700 1 $aGONCALVES, R. C.
700 1 $aALVARES, V. de S.
700 1 $aBITTENCOURT, D. M. de C.
700 1 $aMILLER, R. N. G.
773 $tBMC Microbiology$gv.14, n. 138, 2014.
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Registro original: |
Embrapa Acre (CPAF-AC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
01/11/2018 |
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Data da última atualização: |
01/11/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SILVA, T. R. e; DUARTE, A. W. F.; PASSARINI, M. R. Z.; RUIZ, A. L. T. G.; FRANCO, C. H.; MORAES, C. B.; MELO, I. S. de; RODRIGUES, R. A.; FANTINATTI-GARBOGGINI, F.; OLIVEIRA, V. M. |
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Afiliação: |
TIAGO RODRIGUES E SILVA, IB-UNICAMP; ALYSSON WAGNER FERNANDES DUARTE, UFAL; MICHEL RODRIGO ZAMBRANO PASSARINI, UFAL; ANA LUCIA TASCA GOIS RUIZ, FCF-UNICAMP; CAIO HADDAD FRANCO, CNPEM; CAROLINA BORSOI MORAES, CNPEM; ITAMAR SOARES DE MELO, CNPMA; RODNEY ALEXANDRE RODRIGUES, CPQBA-UNICAMP; FABIANA FANTINATTI-GARBOGGINI, CPQBA-UNICAMP. |
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Título: |
Bacteria from Antarctic environments: diversity and detection of antimicrobial, antiproliferative, and antiparasitic activities. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Polar Biology, v. 41, n. 7, p. 1505-1519, 2018. |
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ISSN: |
1432-2056 |
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DOI: |
https://doi.org/10.1007/s00300-018-2300-y |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Microorganisms dominate most of Antarctic ecosystems and play a crucial role in their functioning. They are called extremophilic microorganisms with unique and versatile metabolic properties with possible biotechnological applications in several areas. The aim of the present study was to identify psychrotolerant microorganisms from Antarctic continent samples and to screen them for antimicrobial effects. Phylogenetic analyses revealed that most isolates were closely related to recognized species, including those recovered previously from Antarctica, which belonged to the major phyla Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteria (classes Alpha, Beta, and Gammaproteobacteria). A total of 326 bacterial isolates, distributed in 39 different genera, were recovered and identified based on sequencing of the 16S rRNA gene. The main representative genera were Arthrobacter, Psychrobacter, Pseudoalteromonas, and Rhodococcus. Antimicrobial screening revealed fifteen isolates capable of inhibiting growth of at least one of the indicator strains: Escherichia coli, Micrococcus luteus, Staphylococcus aureus, Bacillus subtilis, and Candida albicans. One psychrotolerant bacterium, Pseudomonas sp. isolate 99, showed a broad antimicrobial range, in addition to antiproliferative and antiparasitic activity. Overall, the small number of antibiotic-producing isolates obtained and the weakness of their inhibition halos corroborated previous findings suggesting that cold-loving bacteria from Antarctica are not as good as their relatives from mesophilic environments for antimicrobial prospecting. Nonetheless, antiproliferative and antiparasitic results observed are promising and suggest that there is an untapped wealth in Antarctic environments for bioprospecting compounds with pharmaceutical potential application. MenosAbstract: Microorganisms dominate most of Antarctic ecosystems and play a crucial role in their functioning. They are called extremophilic microorganisms with unique and versatile metabolic properties with possible biotechnological applications in several areas. The aim of the present study was to identify psychrotolerant microorganisms from Antarctic continent samples and to screen them for antimicrobial effects. Phylogenetic analyses revealed that most isolates were closely related to recognized species, including those recovered previously from Antarctica, which belonged to the major phyla Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteria (classes Alpha, Beta, and Gammaproteobacteria). A total of 326 bacterial isolates, distributed in 39 different genera, were recovered and identified based on sequencing of the 16S rRNA gene. The main representative genera were Arthrobacter, Psychrobacter, Pseudoalteromonas, and Rhodococcus. Antimicrobial screening revealed fifteen isolates capable of inhibiting growth of at least one of the indicator strains: Escherichia coli, Micrococcus luteus, Staphylococcus aureus, Bacillus subtilis, and Candida albicans. One psychrotolerant bacterium, Pseudomonas sp. isolate 99, showed a broad antimicrobial range, in addition to antiproliferative and antiparasitic activity. Overall, the small number of antibiotic-producing isolates obtained and the weakness of their inhibition halos corroborated previous findings suggesting that cold-lov... Mostrar Tudo |
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Palavras-Chave: |
16S rRNA genes; Bioprospecting; Cold environments. |
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Thesagro: |
Bactéria; Biodiversidade. |
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Thesaurus NAL: |
Antarctica; Antibiotics; Bacillus (bacteria); Bioactive compounds; Pseudomonas. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02966naa a2200373 a 4500
001 2098646
005 2018-11-01
008 2018 bl uuuu u00u1 u #d
022 $a1432-2056
024 7 $ahttps://doi.org/10.1007/s00300-018-2300-y$2DOI
100 1 $aSILVA, T. R. e
245 $aBacteria from Antarctic environments$bdiversity and detection of antimicrobial, antiproliferative, and antiparasitic activities.$h[electronic resource]
260 $c2018
520 $aAbstract: Microorganisms dominate most of Antarctic ecosystems and play a crucial role in their functioning. They are called extremophilic microorganisms with unique and versatile metabolic properties with possible biotechnological applications in several areas. The aim of the present study was to identify psychrotolerant microorganisms from Antarctic continent samples and to screen them for antimicrobial effects. Phylogenetic analyses revealed that most isolates were closely related to recognized species, including those recovered previously from Antarctica, which belonged to the major phyla Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteria (classes Alpha, Beta, and Gammaproteobacteria). A total of 326 bacterial isolates, distributed in 39 different genera, were recovered and identified based on sequencing of the 16S rRNA gene. The main representative genera were Arthrobacter, Psychrobacter, Pseudoalteromonas, and Rhodococcus. Antimicrobial screening revealed fifteen isolates capable of inhibiting growth of at least one of the indicator strains: Escherichia coli, Micrococcus luteus, Staphylococcus aureus, Bacillus subtilis, and Candida albicans. One psychrotolerant bacterium, Pseudomonas sp. isolate 99, showed a broad antimicrobial range, in addition to antiproliferative and antiparasitic activity. Overall, the small number of antibiotic-producing isolates obtained and the weakness of their inhibition halos corroborated previous findings suggesting that cold-loving bacteria from Antarctica are not as good as their relatives from mesophilic environments for antimicrobial prospecting. Nonetheless, antiproliferative and antiparasitic results observed are promising and suggest that there is an untapped wealth in Antarctic environments for bioprospecting compounds with pharmaceutical potential application.
650 $aAntarctica
650 $aAntibiotics
650 $aBacillus (bacteria)
650 $aBioactive compounds
650 $aPseudomonas
650 $aBactéria
650 $aBiodiversidade
653 $a16S rRNA genes
653 $aBioprospecting
653 $aCold environments
700 1 $aDUARTE, A. W. F.
700 1 $aPASSARINI, M. R. Z.
700 1 $aRUIZ, A. L. T. G.
700 1 $aFRANCO, C. H.
700 1 $aMORAES, C. B.
700 1 $aMELO, I. S. de
700 1 $aRODRIGUES, R. A.
700 1 $aFANTINATTI-GARBOGGINI, F.
700 1 $aOLIVEIRA, V. M.
773 $tPolar Biology$gv. 41, n. 7, p. 1505-1519, 2018.
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