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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
08/12/2015 |
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Data da última atualização: |
25/01/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DOMINGOS, D. F.; FARIA, A. F. de; GALAVERNA, R. S.; EBERLIN, M. N.; GREENFIELD, P.; ZUCCHI, T. D.; MELO, I. S. de; TRAN-DINH, N.; MIDGLEY, D.; OLIVEIRA, V. M. |
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Afiliação: |
DANIELA FERREIRA DOMINGOS, CPQBA-UNICAMP; ANDREIA FONSECA DE FARIA, Yale University; RENAN DE SOUZA GALAVERNA, IQ-UNICAMP; MARCOS NOGUEIRA EBERLIN, IQ-UNICAMP; PAUL GREENFIELD, CSIRO; TIAGO DOMINGUES ZUCCHI; ITAMAR SOARES DE MELO, CNPMA; NAI TRAN-DINH, CSIRO; DAVID MIDGLEY, CSIRO; VALERIA MAIA DE OLIVEIRA, CPQBA-UNICAMP. |
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Título: |
Genomic and chemical insights into biosurfactant production by the mangrove-derived strain Bacillus safensis CCMA-560. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Applied Microbiology and Biotechnology, Heidelberg, v. 99, n. 7, p. 3155-3167, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Many Bacillus species can produce biosurfactant, although most of the studies on lipopeptide production by this genus have been focused on Bacillus subtilis. Surfactants are broadly used in pharmaceutical, food and petroleum industry, and biological surfactant shows some advantages over the chemical surfactants, such as less toxicity, production from renewable, cheaper feedstocks and development of novel recombinant hyperproducer strains. This study is aimed to unveil the biosurfactant metabolic pathway and chemical composition in Bacillus safensis strain CCMA-560. The whole genome of the CCMA 560 strain was previously sequenced, and with the aid of bioinformatics tools, its biosurfactant metabolic pathway was compared to other pathways of closely related species. Fourier transform infrared (FTIR) and highresolution TOF mass spectrometry (MS) were used to characterize the biosurfactant molecule. B. safensis CCMA-560 metabolic pathway is similar to other Bacillus species; however, some differences in amino acid incorporation were observed, and chemical analyses corroborated the genetic results. The strain CCMA-560 harbours two genes flanked by srfAC and srfAD not present in other Bacillus spp., which can be involved in the production of the analogue gramicidin. FTIR and MS showed that B. safensis CCMA-560 produces a mixture of at least four lipopeptides with seven amino acids incorporated and a fatty acid chain with 14 carbons, which makes this molecule similar to the biosurfactant of Bacillus pumilus, namely, pumilacidin. This is the first report on the biosurfactant production by B. safensis, encompassing the investigation of the metabolic pathway and chemical characterization of the biosurfactant molecule. MenosAbstract: Many Bacillus species can produce biosurfactant, although most of the studies on lipopeptide production by this genus have been focused on Bacillus subtilis. Surfactants are broadly used in pharmaceutical, food and petroleum industry, and biological surfactant shows some advantages over the chemical surfactants, such as less toxicity, production from renewable, cheaper feedstocks and development of novel recombinant hyperproducer strains. This study is aimed to unveil the biosurfactant metabolic pathway and chemical composition in Bacillus safensis strain CCMA-560. The whole genome of the CCMA 560 strain was previously sequenced, and with the aid of bioinformatics tools, its biosurfactant metabolic pathway was compared to other pathways of closely related species. Fourier transform infrared (FTIR) and highresolution TOF mass spectrometry (MS) were used to characterize the biosurfactant molecule. B. safensis CCMA-560 metabolic pathway is similar to other Bacillus species; however, some differences in amino acid incorporation were observed, and chemical analyses corroborated the genetic results. The strain CCMA-560 harbours two genes flanked by srfAC and srfAD not present in other Bacillus spp., which can be involved in the production of the analogue gramicidin. FTIR and MS showed that B. safensis CCMA-560 produces a mixture of at least four lipopeptides with seven amino acids incorporated and a fatty acid chain with 14 carbons, which makes this molecule similar to t... Mostrar Tudo |
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Palavras-Chave: |
Bacillus safensis; Chemical characterizion; Metabolic pathway; Pumilacidin; Surfactant. |
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Thesagro: |
Bacilo. |
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Thesaurus Nal: |
Biosurfactants. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02693naa a2200313 a 4500
001 2030948
005 2016-01-25
008 2015 bl uuuu u00u1 u #d
100 1 $aDOMINGOS, D. F.
245 $aGenomic and chemical insights into biosurfactant production by the mangrove-derived strain Bacillus safensis CCMA-560.$h[electronic resource]
260 $c2015
520 $aAbstract: Many Bacillus species can produce biosurfactant, although most of the studies on lipopeptide production by this genus have been focused on Bacillus subtilis. Surfactants are broadly used in pharmaceutical, food and petroleum industry, and biological surfactant shows some advantages over the chemical surfactants, such as less toxicity, production from renewable, cheaper feedstocks and development of novel recombinant hyperproducer strains. This study is aimed to unveil the biosurfactant metabolic pathway and chemical composition in Bacillus safensis strain CCMA-560. The whole genome of the CCMA 560 strain was previously sequenced, and with the aid of bioinformatics tools, its biosurfactant metabolic pathway was compared to other pathways of closely related species. Fourier transform infrared (FTIR) and highresolution TOF mass spectrometry (MS) were used to characterize the biosurfactant molecule. B. safensis CCMA-560 metabolic pathway is similar to other Bacillus species; however, some differences in amino acid incorporation were observed, and chemical analyses corroborated the genetic results. The strain CCMA-560 harbours two genes flanked by srfAC and srfAD not present in other Bacillus spp., which can be involved in the production of the analogue gramicidin. FTIR and MS showed that B. safensis CCMA-560 produces a mixture of at least four lipopeptides with seven amino acids incorporated and a fatty acid chain with 14 carbons, which makes this molecule similar to the biosurfactant of Bacillus pumilus, namely, pumilacidin. This is the first report on the biosurfactant production by B. safensis, encompassing the investigation of the metabolic pathway and chemical characterization of the biosurfactant molecule.
650 $aBiosurfactants
650 $aBacilo
653 $aBacillus safensis
653 $aChemical characterizion
653 $aMetabolic pathway
653 $aPumilacidin
653 $aSurfactant
700 1 $aFARIA, A. F. de
700 1 $aGALAVERNA, R. S.
700 1 $aEBERLIN, M. N.
700 1 $aGREENFIELD, P.
700 1 $aZUCCHI, T. D.
700 1 $aMELO, I. S. de
700 1 $aTRAN-DINH, N.
700 1 $aMIDGLEY, D.
700 1 $aOLIVEIRA, V. M.
773 $tApplied Microbiology and Biotechnology, Heidelberg$gv. 99, n. 7, p. 3155-3167, 2015.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
08/11/2013 |
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Data da última atualização: |
22/04/2014 |
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Autoria: |
RIBEIRO, C. C. D. U.; FACCINI. J. L. H.; CANCADO, P. H. D.; PIRANDA. E. M.; BARROS-BATTESTI, D. M.; LEITE, R. C. |
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Afiliação: |
CARLA CAROLINA DIAS UZEDO RIBEIRO, Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Serope´dica, RJ; JOÃO LUIZ HORÁCIO FACCINI, Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Serope´dica, RJ; PAULO HENRIQUE DUARTE CANCADO, CNPGC; ELIANE MATTOS PIRANDA, Centro de Ciências Biológicas e da Saúde, UFMS, Campo Grande, MS; DARCI MORAES BARROS-BATTESTI, Laboratório de Parasitologia, Instituto Butantan, São Paulo, SP; ROMÁRIO CERQUEIRA LEITE, Escola de Veterinária, Universidade Federal de Minas Gerais, Minas Gerais, MG. |
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Título: |
Life cycle of Ornithodoros rostratus (Acari: Argasidae) under experimental conditions and comments on the host-parasite relationship in the Pantanal wetland region, Brazil |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Experimental and Applied Acarology, v. 61, 2013, p. 139-146. |
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Idioma: |
Português |
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Palavras-Chave: |
Experimental; Host-relations; Life cycle; Ornithodoros rostratus. |
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Thesaurus NAL: |
Argasidae. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/92223/1/Ribeiro-et-al.2013-1.pdf
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Marc: |
LEADER 00787naa a2200229 a 4500
001 1970848
005 2014-04-22
008 2013 bl uuuu u00u1 u #d
100 1 $aRIBEIRO, C. C. D. U.
245 $aLife cycle of Ornithodoros rostratus (Acari$bArgasidae) under experimental conditions and comments on the host-parasite relationship in the Pantanal wetland region, Brazil$h[electronic resource]
260 $c2013
650 $aArgasidae
653 $aExperimental
653 $aHost-relations
653 $aLife cycle
653 $aOrnithodoros rostratus
700 1 $aFACCINI. J. L. H.
700 1 $aCANCADO, P. H. D.
700 1 $aPIRANDA. E. M.
700 1 $aBARROS-BATTESTI, D. M.
700 1 $aLEITE, R. C.
773 $tExperimental and Applied Acarology$gv. 61, 2013, p. 139-146.
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