Data/hora: 11/06/2024 15:12:40

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 16/02/2016
Data da última atualização: | 29/12/2017
Autoria: | METTIFOGO, E.; BUZINHANI, M.; BUIM, M. R.; TIMENETSKY, J.; FERREIRA, A. J. P.
Afiliação: | ELENA METTIFOGO, ICBM/USP; MELISSA BUZINHANI, ICBM/USP; MARCOS R. BUIM, Instituto Biológico; JORGE TIMENETSKY, ICBM/USP; ANTONIO J. PIANTINO FERREIRA, USP.
Título: | Evaluation of a PCR multiplex for detection and differentiation of Mycoplasma synoviae, M. gallisepticum, and M. gallisepticum strain F-vaccine.
Ano de publicação: | 2015
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 35, n. 1, p. 13-18, jan. 2015.
Idioma: | Inglês
Notas: | Título em português: Avaliação de uma PCR multiplex para detecção e diferenciação de Mycoplasma  synoviae, Mycoplasma gallisepticum e Mycoplasma gallisepticum cepa F vacinal.
Conteúdo: | Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are the mycoplasma infections of most concern for commercial poultry industry. MG infection is commonly designated as chronic respiratory disease (CRD) of chickens and infections sinusitis of turkeys. MS causes sub clinical upper respiratory infection and tenosynovitis or bursitis in chickens and turkeys. The multiplex PCR was standardized to detect simultaneously the MS, MG field strains and MG F-vaccine strain specific. The generic PCR for detection of any species of Mollicutes Class was performed and compared to the multiplex PCR and to PCR using species-specific primers. A total of 129 avian tracheal swabs were collected from broiler-breeders, layer hens and broilers in seven different farms and were examined by multiplex PCR methods. The system (multiplex PCR) demonstrated to be very rapid, sensitive, and specific. Therefore, the results showed a high prevalence of MS in the flocks examined (27.9%), and indicate that the MS is a recurrent pathogen in Brazilian commercial poultry flocks.
Palavras-Chave: | Multiplex PCR.
Thesagro: | Mycoplasma Gallisepticum.
Thesaurus Nal: | Chickens.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/139127/1/Evaluation-of-a-PCR-multiplex.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (58796-1) - UPE - AP - PP - 636.08905 - P474 -  - DIS