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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
07/04/2004 |
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Data da última atualização: |
23/04/2012 |
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Autoria: |
WENDLAND, A.; SILVA, J. F. V.; BINNECK, E.; MARIN, S. R. R.; MORALES, A. M. R.; TRAVENSOLO, R. F.; ALVES, L. C.; LEMOS, E. G. M.; MENTEN, J. O. M.; NEPOMUCENO, A. L. |
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Título: |
Genetic expression of soybeans: Meloidogyne javanica interaction, revealed by analyses of microarrays. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
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Páginas: |
p. 320. |
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Série: |
(Embrapa Soja. Documentos, 228). |
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Idioma: |
Inglês |
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Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. |
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Conteúdo: |
The root-knot nematode, Meloidogyne javanica is an economically important pathogen of soybeans. Effective management of the nematode is often dependent on the use of resistant genotypes. The genetic interactions are complex and poorly understood. The use of cDNA Microarray technology promises to accelerate rapidly our understand of infectious disease processes, by allowing the examination of thousands of genes and gene products, simultaneously. To explore the plant genes putatively involved in the events of nematode-plant parasitism, we used Affymetrix GeneChips to conduct expression profiling of the early stages of infection of soybean genotypes. The infection experiments were conducted with three treatments: mock infected, resistant soybean genotype JF 7056 infected and suscetible soybean genotype 266-S infected with second stage juveniles - J2 of M. javanica. RNA obtained from the roots of each treatment was used to construct cDNA libraries, used for late sequencing. About 1000 unique sequences cDNA were chosen to be plotted in Microrray slides. Target cDNA was labelled by reverse transcription and incorporated with aminoallyl modified dUTP. A total of six hibridizations were performed. Sequences analyzed identified genes related to nematode resistance (ex: gene Hs1pro -1), and other stresses. |
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Thesagro: |
Nematóide. |
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Categoria do assunto: |
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Marc: |
LEADER 02463naa a2200277 a 4500
001 1466851
005 2012-04-23
008 2004 bl uuuu u00u1 u #d
100 1 $aWENDLAND, A.
245 $aGenetic expression of soybeans$bMeloidogyne javanica interaction, revealed by analyses of microarrays.
260 $c2004
300 $ap. 320.
490 $a(Embrapa Soja. Documentos, 228).
500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
520 $aThe root-knot nematode, Meloidogyne javanica is an economically important pathogen of soybeans. Effective management of the nematode is often dependent on the use of resistant genotypes. The genetic interactions are complex and poorly understood. The use of cDNA Microarray technology promises to accelerate rapidly our understand of infectious disease processes, by allowing the examination of thousands of genes and gene products, simultaneously. To explore the plant genes putatively involved in the events of nematode-plant parasitism, we used Affymetrix GeneChips to conduct expression profiling of the early stages of infection of soybean genotypes. The infection experiments were conducted with three treatments: mock infected, resistant soybean genotype JF 7056 infected and suscetible soybean genotype 266-S infected with second stage juveniles - J2 of M. javanica. RNA obtained from the roots of each treatment was used to construct cDNA libraries, used for late sequencing. About 1000 unique sequences cDNA were chosen to be plotted in Microrray slides. Target cDNA was labelled by reverse transcription and incorporated with aminoallyl modified dUTP. A total of six hibridizations were performed. Sequences analyzed identified genes related to nematode resistance (ex: gene Hs1pro -1), and other stresses.
650 $aNematóide
700 1 $aSILVA, J. F. V.
700 1 $aBINNECK, E.
700 1 $aMARIN, S. R. R.
700 1 $aMORALES, A. M. R.
700 1 $aTRAVENSOLO, R. F.
700 1 $aALVES, L. C.
700 1 $aLEMOS, E. G. M.
700 1 $aMENTEN, J. O. M.
700 1 $aNEPOMUCENO, A. L.
773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
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Registro original: |
Embrapa Soja (CNPSO) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
03/10/2013 |
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Data da última atualização: |
03/10/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
AMARANTE, C. V. T. do; MIQUELOTO, A.; FREITAS, S. T. de; STEFFENS, C. A.; SILVEIRA, J. P. G.; CORRÊA, T. R. |
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Afiliação: |
CASSANDRO VIDAL TALAMINI DO AMARANTE, Universidade do Estado de Santa Catarina; AQUIDAUANA MIQUELOTO, Universidade do Estado de Santa Catarina; SERGIO TONETTO DE FREITAS, CPATSA; CRISTIANO ANDRÉ STEFFENS, Universidade do Estado de Santa Catarina; JOÃO PAULO GENEROSO SILVEIRA, Universidade do Estado de Santa Catarina; THAIS ROSELI CORRÊA, Universidade do Estado de Santa Catarina. |
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Título: |
Fruit sampling methods to quantify calcium and magnesium contents to predict bitter pit development in Fuji apple: A multivariate approach. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Scientia Horticulturae, Amsterdam, v. 157, p. 19-23, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
Bitter pit (BP) is a physiological disorder in apple commonly associated with high Mg/Ca ratio in fruit tissue. This work was carried out to identify the best fruit tissue sampling method for Ca and Mg assessment to discriminate ?Fuji? apples regarding the occurrence of BP. After six months under controlled atmosphere storage (2.0 kPa O2 + 0.5 kPa CO2, at 0.5 ?C/90?95% RH), fruit without or with BP were submitted to Ca and Mg analysis (mg kg?1 of fresh weight) in the total fraction (TF) of peel + flesh, and in the TF and soluble fraction (SF) of flesh or peel tissues. For the peel + flesh, a wedge-shaped segment was cut longitudinally from the fruit (with 1 cm wide at the equatorial region), discarding the core tissue. For individual sampling of the peel (thickness of 0?2 mm) or flesh (thickness of 2?8 mm), the fruit were cut along the equatorial region, so that only the distal end was used. In this portion of the fruit, Ca and Mg contents were higher in the peel than in the flesh in the TF, and lower in the peel than in the flesh in the SF, while the Mg/Ca ratios in TF and SF fractions were higher in the flesh than in the peel, regardless the occurrence of BP. Calcium and Mg contents in the SF relative to the TF were very low in both peel and flesh tissues. Calcium in the SF represented 0.36% and 2.79% of its content in the TF for peel and flesh, respectively. Magnesium content in the SF represented 0.70% and 3.74% of that in the TF for peel and flesh, respectively. Fruit without BP showed higher Ca content and lower Mg/Ca ratio in the TF of peel + flesh, peel or flesh, and in the SF of peel or flesh, compared to fruit with BP. Fruit without BP also showed a higher percentage of Ca in the SF in relation to TF in the peel (0.42%) compared to fruit with BP (0.31%). Fruit without BP also showed lower Mg content in TF of peel + flesh and peel, and in the SF of the flesh. There was no difference between fruit with and without BP only for Mg contents in TF of the flesh and SF of the peel. The canonical discriminant analysis showed that the Mg/Ca ratio in the SF of the peel tissue at the distal end of the fruit provides the best method to discriminate fruit without and with BP, which can be potentially used as a tool to predict BP development in Fuji apples. MenosBitter pit (BP) is a physiological disorder in apple commonly associated with high Mg/Ca ratio in fruit tissue. This work was carried out to identify the best fruit tissue sampling method for Ca and Mg assessment to discriminate ?Fuji? apples regarding the occurrence of BP. After six months under controlled atmosphere storage (2.0 kPa O2 + 0.5 kPa CO2, at 0.5 ?C/90?95% RH), fruit without or with BP were submitted to Ca and Mg analysis (mg kg?1 of fresh weight) in the total fraction (TF) of peel + flesh, and in the TF and soluble fraction (SF) of flesh or peel tissues. For the peel + flesh, a wedge-shaped segment was cut longitudinally from the fruit (with 1 cm wide at the equatorial region), discarding the core tissue. For individual sampling of the peel (thickness of 0?2 mm) or flesh (thickness of 2?8 mm), the fruit were cut along the equatorial region, so that only the distal end was used. In this portion of the fruit, Ca and Mg contents were higher in the peel than in the flesh in the TF, and lower in the peel than in the flesh in the SF, while the Mg/Ca ratios in TF and SF fractions were higher in the flesh than in the peel, regardless the occurrence of BP. Calcium and Mg contents in the SF relative to the TF were very low in both peel and flesh tissues. Calcium in the SF represented 0.36% and 2.79% of its content in the TF for peel and flesh, respectively. Magnesium content in the SF represented 0.70% and 3.74% of that in the TF for peel and flesh, respectively. Fruit w... Mostrar Tudo |
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Palavras-Chave: |
Análise multivariada; Apple; Composição mineral; Desordem fisiológica; Metodologia de amostragem; Ppp. |
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Thesagro: |
Cálcio; Fisiologia; Magnésio. |
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Thesaurus NAL: |
bitter pit. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 03207naa a2200301 a 4500
001 1967747
005 2013-10-03
008 2013 bl uuuu u00u1 u #d
100 1 $aAMARANTE, C. V. T. do
245 $aFruit sampling methods to quantify calcium and magnesium contents to predict bitter pit development in Fuji apple$bA multivariate approach.$h[electronic resource]
260 $c2013
520 $aBitter pit (BP) is a physiological disorder in apple commonly associated with high Mg/Ca ratio in fruit tissue. This work was carried out to identify the best fruit tissue sampling method for Ca and Mg assessment to discriminate ?Fuji? apples regarding the occurrence of BP. After six months under controlled atmosphere storage (2.0 kPa O2 + 0.5 kPa CO2, at 0.5 ?C/90?95% RH), fruit without or with BP were submitted to Ca and Mg analysis (mg kg?1 of fresh weight) in the total fraction (TF) of peel + flesh, and in the TF and soluble fraction (SF) of flesh or peel tissues. For the peel + flesh, a wedge-shaped segment was cut longitudinally from the fruit (with 1 cm wide at the equatorial region), discarding the core tissue. For individual sampling of the peel (thickness of 0?2 mm) or flesh (thickness of 2?8 mm), the fruit were cut along the equatorial region, so that only the distal end was used. In this portion of the fruit, Ca and Mg contents were higher in the peel than in the flesh in the TF, and lower in the peel than in the flesh in the SF, while the Mg/Ca ratios in TF and SF fractions were higher in the flesh than in the peel, regardless the occurrence of BP. Calcium and Mg contents in the SF relative to the TF were very low in both peel and flesh tissues. Calcium in the SF represented 0.36% and 2.79% of its content in the TF for peel and flesh, respectively. Magnesium content in the SF represented 0.70% and 3.74% of that in the TF for peel and flesh, respectively. Fruit without BP showed higher Ca content and lower Mg/Ca ratio in the TF of peel + flesh, peel or flesh, and in the SF of peel or flesh, compared to fruit with BP. Fruit without BP also showed a higher percentage of Ca in the SF in relation to TF in the peel (0.42%) compared to fruit with BP (0.31%). Fruit without BP also showed lower Mg content in TF of peel + flesh and peel, and in the SF of the flesh. There was no difference between fruit with and without BP only for Mg contents in TF of the flesh and SF of the peel. The canonical discriminant analysis showed that the Mg/Ca ratio in the SF of the peel tissue at the distal end of the fruit provides the best method to discriminate fruit without and with BP, which can be potentially used as a tool to predict BP development in Fuji apples.
650 $abitter pit
650 $aCálcio
650 $aFisiologia
650 $aMagnésio
653 $aAnálise multivariada
653 $aApple
653 $aComposição mineral
653 $aDesordem fisiológica
653 $aMetodologia de amostragem
653 $aPpp
700 1 $aMIQUELOTO, A.
700 1 $aFREITAS, S. T. de
700 1 $aSTEFFENS, C. A.
700 1 $aSILVEIRA, J. P. G.
700 1 $aCORRÊA, T. R.
773 $tScientia Horticulturae, Amsterdam$gv. 157, p. 19-23, 2013.
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