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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sul. |
Data corrente: |
13/01/2020 |
Data da última atualização: |
15/01/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FAST HINZ, O.; CHILIBROSTE, P.; MENEGAZZI, G.; OBORSKY, M.; GENRO, T. C. M.; SOCA, P.; MATTIAUDA, D. A. |
Afiliação: |
Oliver Fast Hinz, Universidad de la República; Pablo Chilibroste, Universidad de la República; Gabriel Menegazzi, Universidad de la República; Matías Oborsky, Universidad de la República; TERESA CRISTINA MORAES GENRO, CPPSUL; Pablo Soca, Universidad de la República; Diego A. Mattiauda, Universidad de la República. |
Título: |
How is the ingestive behaviour of mid lactating Holstein cows grazing a fescue based pasture under two different defoliation intensities? |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Journal of Animal Science, v. 97, p. 385-386, Dec. 2019. |
DOI: |
doi.org/10.1093/jas/skz258.766 |
Idioma: |
Inglês |
Notas: |
Supplement 3. ASAS-CSAS Annual Meeting and Trade Show. |
Conteúdo: |
An experiment was performed to study the effect of two contrasting defoliation intensities of a fescue based pasture on the ingestive behaviour. Treatments were two grazing intensities: lax treatment (TL) 12 cm and control treatment (TC) 6 cm, with four replicants of 0.3 ha each. The starting of the experiment was determined by the three leaf stage. Twenty-four mid lactating Holstein cows were blocked according to parity, body weight, body condition score and calving date. Each plot was grazed by three cows during as many days as necessary until the defoliation height of each treatment was achieved (TL: 6 d; TC: 8 d). The pasture mass was 2530 ± 180 kgDM/ha and animals had access from 8:00 to 16:00. Each cow received 7.4 kgDM of supplementation (17% Crude Protein; 2.81 Mcal/kgDM). After PM milking (17:00 h) animals remained separated in 8 pens, where they received 5.5 kgDM in individual troughs. The remaining supplement was provided in the AM milking (05:00 h). During the access to the pasture, grazing and ruminating times and bites rate (BT; bites/minute) were determined every 5 minutes by visual observation at the beginning, middle and end of the experiment. Daily ruminating (DR) was also logged by electronic recorders in 2h periods (Heatime®HR, SCR Dairy). The PROC GLIMMIX of SAS (SAS 9.2, 2010) with a binomial response distribution and with Logit as a link function, was used to determine the probability of the different events. DR and BR were analysed with a mixed model with block and treatment as fixed effects. The probability of cows grazing was greater for TC than TL and lower for ruminating and DR (Table 1). There was no effect of treatment on BR (55 ± 8.8 bites/min). The changes observed on ingestive behaviour were reflected on productive performance (companion abstract). MenosAn experiment was performed to study the effect of two contrasting defoliation intensities of a fescue based pasture on the ingestive behaviour. Treatments were two grazing intensities: lax treatment (TL) 12 cm and control treatment (TC) 6 cm, with four replicants of 0.3 ha each. The starting of the experiment was determined by the three leaf stage. Twenty-four mid lactating Holstein cows were blocked according to parity, body weight, body condition score and calving date. Each plot was grazed by three cows during as many days as necessary until the defoliation height of each treatment was achieved (TL: 6 d; TC: 8 d). The pasture mass was 2530 ± 180 kgDM/ha and animals had access from 8:00 to 16:00. Each cow received 7.4 kgDM of supplementation (17% Crude Protein; 2.81 Mcal/kgDM). After PM milking (17:00 h) animals remained separated in 8 pens, where they received 5.5 kgDM in individual troughs. The remaining supplement was provided in the AM milking (05:00 h). During the access to the pasture, grazing and ruminating times and bites rate (BT; bites/minute) were determined every 5 minutes by visual observation at the beginning, middle and end of the experiment. Daily ruminating (DR) was also logged by electronic recorders in 2h periods (Heatime®HR, SCR Dairy). The PROC GLIMMIX of SAS (SAS 9.2, 2010) with a binomial response distribution and with Logit as a link function, was used to determine the probability of the different events. DR and BR were analysed with a mixed model ... Mostrar Tudo |
Thesagro: |
Festuca; Gado Holandês; Ingestão; Lactação. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02665naa a2200265 a 4500 001 2118640 005 2020-01-15 008 2019 bl uuuu u00u1 u #d 024 7 $adoi.org/10.1093/jas/skz258.766$2DOI 100 1 $aFAST HINZ, O. 245 $aHow is the ingestive behaviour of mid lactating Holstein cows grazing a fescue based pasture under two different defoliation intensities?$h[electronic resource] 260 $c2019 500 $aSupplement 3. ASAS-CSAS Annual Meeting and Trade Show. 520 $aAn experiment was performed to study the effect of two contrasting defoliation intensities of a fescue based pasture on the ingestive behaviour. Treatments were two grazing intensities: lax treatment (TL) 12 cm and control treatment (TC) 6 cm, with four replicants of 0.3 ha each. The starting of the experiment was determined by the three leaf stage. Twenty-four mid lactating Holstein cows were blocked according to parity, body weight, body condition score and calving date. Each plot was grazed by three cows during as many days as necessary until the defoliation height of each treatment was achieved (TL: 6 d; TC: 8 d). The pasture mass was 2530 ± 180 kgDM/ha and animals had access from 8:00 to 16:00. Each cow received 7.4 kgDM of supplementation (17% Crude Protein; 2.81 Mcal/kgDM). After PM milking (17:00 h) animals remained separated in 8 pens, where they received 5.5 kgDM in individual troughs. The remaining supplement was provided in the AM milking (05:00 h). During the access to the pasture, grazing and ruminating times and bites rate (BT; bites/minute) were determined every 5 minutes by visual observation at the beginning, middle and end of the experiment. Daily ruminating (DR) was also logged by electronic recorders in 2h periods (Heatime®HR, SCR Dairy). The PROC GLIMMIX of SAS (SAS 9.2, 2010) with a binomial response distribution and with Logit as a link function, was used to determine the probability of the different events. DR and BR were analysed with a mixed model with block and treatment as fixed effects. The probability of cows grazing was greater for TC than TL and lower for ruminating and DR (Table 1). There was no effect of treatment on BR (55 ± 8.8 bites/min). The changes observed on ingestive behaviour were reflected on productive performance (companion abstract). 650 $aFestuca 650 $aGado Holandês 650 $aIngestão 650 $aLactação 700 1 $aCHILIBROSTE, P. 700 1 $aMENEGAZZI, G. 700 1 $aOBORSKY, M. 700 1 $aGENRO, T. C. M. 700 1 $aSOCA, P. 700 1 $aMATTIAUDA, D. A. 773 $tJournal of Animal Science$gv. 97, p. 385-386, Dec. 2019.
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Embrapa Pecuária Sul (CPPSUL) |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
14/06/2010 |
Data da última atualização: |
01/11/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
NOBILE, P. M.; QUECINI, V.; BAZZO, B.; QUITERIO, G.; MAZZAFERA, P.; COLOMBO, C. A. |
Afiliação: |
PAULA M. NOBILE, IAC; VERA MARIA QUECINI, CNPUV; BARBARA BAZZO, IAC; GABRIELA QUITERIO, IAC; PAULO MAZZAFERA, INSTITUTO DE BIOLOGIA - UNICAMP; CARLOS A. COLOMBO, IAC. |
Título: |
Transcriptional profile of genes involved in the byosynthesis of phytate and ferritin in coffea. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Journal of Agricultural and Food Chemistry, Washington DC, v. 58, n. 6, p. 3479-3487, mar. 2010. |
DOI: |
10.1021/jf9043088 |
Idioma: |
Inglês |
Conteúdo: |
The present work aimed to study the control of the biosynthesis of the antinutritional factor phytate and its associated Fe-rich protein family, ferritin, in coffee. Phytate has the ability to chelate Fe, making it unavailable to human absorption. The Coffea genome databases were queried for genes associated with phytate metabolism and ferritin genes. The genetic framework for phytate biosynthesis and its reverse pathway was identified in silico analyses and indicate that Coffea phosphatidyl inositol kinase and monophosphatase families play nonredundant roles in phytate metabolism. The transcriptional profiles of phytate biosynthesis key-genes MYO-INOSITOL(3)P1 SYNTHASE, two genes coding for PHOSPHATIDYL INOSITOL KINASE, and three FERRITIN genes were temporally evaluated by qPCR in coffee seeds from two crop locations, Adamantina-SP and Ouro-Fino-MG, the last one traditionally associated with high-quality coffee beverage grain. A targeted metabolome profile of phytic acid contents throughout three fruit maturation stages in association with the transcriptional analysis was also obtained. Taken together, our data indicate that the investigated local conditions did not cause significant alterations in phytate biosynthesis. Futhermore, the temporal transcriptional profiling revealed that candidate gene expression is regulated independently of phytate accumulation. In contrast, the expression profile of ferritin-unit genes is affected by environmental conditions and genetic background. The roles of the investigated genes are discussed concerning the quality of coffee beverage. MenosThe present work aimed to study the control of the biosynthesis of the antinutritional factor phytate and its associated Fe-rich protein family, ferritin, in coffee. Phytate has the ability to chelate Fe, making it unavailable to human absorption. The Coffea genome databases were queried for genes associated with phytate metabolism and ferritin genes. The genetic framework for phytate biosynthesis and its reverse pathway was identified in silico analyses and indicate that Coffea phosphatidyl inositol kinase and monophosphatase families play nonredundant roles in phytate metabolism. The transcriptional profiles of phytate biosynthesis key-genes MYO-INOSITOL(3)P1 SYNTHASE, two genes coding for PHOSPHATIDYL INOSITOL KINASE, and three FERRITIN genes were temporally evaluated by qPCR in coffee seeds from two crop locations, Adamantina-SP and Ouro-Fino-MG, the last one traditionally associated with high-quality coffee beverage grain. A targeted metabolome profile of phytic acid contents throughout three fruit maturation stages in association with the transcriptional analysis was also obtained. Taken together, our data indicate that the investigated local conditions did not cause significant alterations in phytate biosynthesis. Futhermore, the temporal transcriptional profiling revealed that candidate gene expression is regulated independently of phytate accumulation. In contrast, the expression profile of ferritin-unit genes is affected by environmental conditions and genetic back... Mostrar Tudo |
Palavras-Chave: |
Fator anti-nutricional; Ferritina; Fitato. |
Thesagro: |
Biologia; Café; Ferro; Genética. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/204172/1/12303-2010-p.3479-3487-2.pdf
|
Marc: |
LEADER 02404naa a2200277 a 4500 001 1854944 005 2019-11-01 008 2010 bl uuuu u00u1 u #d 024 7 $a10.1021/jf9043088$2DOI 100 1 $aNOBILE, P. M. 245 $aTranscriptional profile of genes involved in the byosynthesis of phytate and ferritin in coffea.$h[electronic resource] 260 $c2010 520 $aThe present work aimed to study the control of the biosynthesis of the antinutritional factor phytate and its associated Fe-rich protein family, ferritin, in coffee. Phytate has the ability to chelate Fe, making it unavailable to human absorption. The Coffea genome databases were queried for genes associated with phytate metabolism and ferritin genes. The genetic framework for phytate biosynthesis and its reverse pathway was identified in silico analyses and indicate that Coffea phosphatidyl inositol kinase and monophosphatase families play nonredundant roles in phytate metabolism. The transcriptional profiles of phytate biosynthesis key-genes MYO-INOSITOL(3)P1 SYNTHASE, two genes coding for PHOSPHATIDYL INOSITOL KINASE, and three FERRITIN genes were temporally evaluated by qPCR in coffee seeds from two crop locations, Adamantina-SP and Ouro-Fino-MG, the last one traditionally associated with high-quality coffee beverage grain. A targeted metabolome profile of phytic acid contents throughout three fruit maturation stages in association with the transcriptional analysis was also obtained. Taken together, our data indicate that the investigated local conditions did not cause significant alterations in phytate biosynthesis. Futhermore, the temporal transcriptional profiling revealed that candidate gene expression is regulated independently of phytate accumulation. In contrast, the expression profile of ferritin-unit genes is affected by environmental conditions and genetic background. The roles of the investigated genes are discussed concerning the quality of coffee beverage. 650 $aBiologia 650 $aCafé 650 $aFerro 650 $aGenética 653 $aFator anti-nutricional 653 $aFerritina 653 $aFitato 700 1 $aQUECINI, V. 700 1 $aBAZZO, B. 700 1 $aQUITERIO, G. 700 1 $aMAZZAFERA, P. 700 1 $aCOLOMBO, C. A. 773 $tJournal of Agricultural and Food Chemistry, Washington DC$gv. 58, n. 6, p. 3479-3487, mar. 2010.
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