| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Meio Ambiente. |
|
Data corrente: |
11/02/2016 |
|
Data da última atualização: |
12/02/2016 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
MENDES, L. W.; MENDES, R.; TSAI, S. M. |
|
Afiliação: |
L. W. MENDES, ESALQ/USP; RODRIGO MENDES, CNPMA; S. M. TSAI, ESALQ/USP. |
|
Título: |
Metagenomic analysis of the rhizosphere microbiome of the common bean resistant to Fusarium oxysporum. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
In: RHIZOSPHERE, 4., 2015, Maastricht. Stretching the interface of life: abstracts... Maastricht: Wageningen University & Research Centre and the Netherlands Institute of Ecology, 2015. Ref. 56. |
|
Páginas: |
34-35 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The rhizosphere microbiome plays a key role in the functioning of the host plant, influencing its physiology and development. It has been suggested that plants use mechanisms present in the rhizosphere microbiome to fend off infections, such as fungal diseases. This work aimed to assess the microbial community inhabiting the common bean rhizosphere in order to identify potential groups related to the suppression of the soil-borne pathogen Fusarium oxysporum. Therefore, using shotgun metagenomic sequencing (Illumina Miseq), we investigated the phylogenetic and potential functional diversity of microbial communities colonizing the rhizosphere of four cultivars of common bean with different levels of resistance to the fungus, ranging from high susceptibility to resistant. Quantitative PCR of total bacteria in rhizosphere samples showed in increase of 16S rRNA copy number with the increase of resistance to the fungus. Mesocosms experiments, including four common bean cultivars cultivated in Amazonian Dark Earth and three replicates, were conducted in greenhouse conditions and we obtained over than 12 million metagenomic sequences. The overall microbial diversity did not present significant variations across common bean cultivars. From the classified sequences, 97,4% were affiliated to Bacteria and 1,48% to Archaea. Proteobacteria represented the most abundant phyla (41,7%), followed by Actinobacteria (29,4%), Firmicutes (5,9%) and Acidobacteria (4,1%). The microbial communities structure were different between bulk soil and rhizosphere samples. Comparing all bean cultivars, the resistant one showed an overrepresention of the phyla Spirochaetes, Nitrospirae and Euryarchaeota. The resistant bean cultivar presented high number of sequences affiliated to the genus Bacillus. Interestingly, the resistant and moderately resistant cultivars, presented high proportion of sequences related to bacteriocin, a narrow spectrum antibiotics. Preliminary analysis showed that different common bean cultivars could select differential microbial groups in the rhizosphere environment. Further analysis will search for bacterial groups potentially related to the fungal antagonism. MenosThe rhizosphere microbiome plays a key role in the functioning of the host plant, influencing its physiology and development. It has been suggested that plants use mechanisms present in the rhizosphere microbiome to fend off infections, such as fungal diseases. This work aimed to assess the microbial community inhabiting the common bean rhizosphere in order to identify potential groups related to the suppression of the soil-borne pathogen Fusarium oxysporum. Therefore, using shotgun metagenomic sequencing (Illumina Miseq), we investigated the phylogenetic and potential functional diversity of microbial communities colonizing the rhizosphere of four cultivars of common bean with different levels of resistance to the fungus, ranging from high susceptibility to resistant. Quantitative PCR of total bacteria in rhizosphere samples showed in increase of 16S rRNA copy number with the increase of resistance to the fungus. Mesocosms experiments, including four common bean cultivars cultivated in Amazonian Dark Earth and three replicates, were conducted in greenhouse conditions and we obtained over than 12 million metagenomic sequences. The overall microbial diversity did not present significant variations across common bean cultivars. From the classified sequences, 97,4% were affiliated to Bacteria and 1,48% to Archaea. Proteobacteria represented the most abundant phyla (41,7%), followed by Actinobacteria (29,4%), Firmicutes (5,9%) and Acidobacteria (4,1%). The microbial communities ... Mostrar Tudo |
|
Palavras-Chave: |
Metagenômica. |
|
Thesagro: |
Feijão; Fusarium Oxysporum. |
|
Thesaurus Nal: |
Metagenomics. |
|
Categoria do assunto: |
S Ciências Biológicas |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/138807/1/2015RA-067.pdf
|
|
Marc: |
LEADER 02914nam a2200193 a 4500
001 2036658
005 2016-02-12
008 2015 bl uuuu u00u1 u #d
100 1 $aMENDES, L. W.
245 $aMetagenomic analysis of the rhizosphere microbiome of the common bean resistant to Fusarium oxysporum.$h[electronic resource]
260 $aIn: RHIZOSPHERE, 4., 2015, Maastricht. Stretching the interface of life: abstracts... Maastricht: Wageningen University & Research Centre and the Netherlands Institute of Ecology, 2015. Ref. 56.$c2015
300 $a34-35
520 $aThe rhizosphere microbiome plays a key role in the functioning of the host plant, influencing its physiology and development. It has been suggested that plants use mechanisms present in the rhizosphere microbiome to fend off infections, such as fungal diseases. This work aimed to assess the microbial community inhabiting the common bean rhizosphere in order to identify potential groups related to the suppression of the soil-borne pathogen Fusarium oxysporum. Therefore, using shotgun metagenomic sequencing (Illumina Miseq), we investigated the phylogenetic and potential functional diversity of microbial communities colonizing the rhizosphere of four cultivars of common bean with different levels of resistance to the fungus, ranging from high susceptibility to resistant. Quantitative PCR of total bacteria in rhizosphere samples showed in increase of 16S rRNA copy number with the increase of resistance to the fungus. Mesocosms experiments, including four common bean cultivars cultivated in Amazonian Dark Earth and three replicates, were conducted in greenhouse conditions and we obtained over than 12 million metagenomic sequences. The overall microbial diversity did not present significant variations across common bean cultivars. From the classified sequences, 97,4% were affiliated to Bacteria and 1,48% to Archaea. Proteobacteria represented the most abundant phyla (41,7%), followed by Actinobacteria (29,4%), Firmicutes (5,9%) and Acidobacteria (4,1%). The microbial communities structure were different between bulk soil and rhizosphere samples. Comparing all bean cultivars, the resistant one showed an overrepresention of the phyla Spirochaetes, Nitrospirae and Euryarchaeota. The resistant bean cultivar presented high number of sequences affiliated to the genus Bacillus. Interestingly, the resistant and moderately resistant cultivars, presented high proportion of sequences related to bacteriocin, a narrow spectrum antibiotics. Preliminary analysis showed that different common bean cultivars could select differential microbial groups in the rhizosphere environment. Further analysis will search for bacterial groups potentially related to the fungal antagonism.
650 $aMetagenomics
650 $aFeijão
650 $aFusarium Oxysporum
653 $aMetagenômica
700 1 $aMENDES, R.
700 1 $aTSAI, S. M.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Meio Ambiente (CNPMA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 189 | |
| 62. |  | SILVA, L. C. da; NASCIMENTO, M. A.; ALVES, C. R.; MENDES, L. G.; FURTADO, R. F.; CARDOSO, A. L. H. Avaliação da formação de microcápsulas de óleo de pequi em matrizes de goma de cajueiro e quitosana por coacervação complexa. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 25., 2016, Gramado. Anais... Gramado: SBCTA-RS, 2016.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
|    |
| 65. | | CASTRO, F. T. de; OLIVEIRA, S. P. de; TABAI, K. C.; GOÉS, H. de A.; MENDES, L. L. Estratégias de venda no comércio de frutas e hortaliças na Zona Oeste do município do Rio de Janeiro. Revista da Associação Brasileira de Nutrição, Rio de Janeiro, v. 1, n. 1, p. 203, maio/jul. 2008. Edição dos Anais do XX Congresso Brasileiro de Nutrição, Rio de Janeiro, maio 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
| |
| 66. | | BRAGA, L. P.; TANENTZAP, A. J.; LEE, B.; TSAI, S. M.; RAAIJMAKERS, J. M.; MENDES, R.; MENDES, L. W. Diversity of viruses and viroids in the rhizosphere of common bean cultivars differing in resistance to the fungal root pathogen Fusarium oxysporum. Applied Soil Ecology, v. 190, article 105018, 2023.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Meio Ambiente. |
| |
| 68. | | ROCHA, C. S.; RITTERBUSCH, G. A.; MORES, N.; SIMON, N. L.; DINIZ-MENDES, L.; NIEL, C.; ZANELLA, J. R. C. Detection of porcine single-stranded DNA viruses in reproductive organs of culled sows. In: INTERNATIONAL PIG VETERINARY SOCIETY(IPVS) CONGRESS, 21., 2010, Vancouver. Proceedings... Vancouver: IPVS, 2010. p. 471. 1 CD-Rom. Projeto/Plano de Ação: 16.0030.044.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
| |
| 69. | | MENDES, L. de M. O.; FILIPPI, M. C. C. de; GUEDES, A. S.; HOFFMANN, L. V.; ARAÚJO, L. G. Genes de avirulência de seis isolados brasileiros de Magnaporthe oryzae de cultivares de terras altas e irrigado. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., 2023, Brasília, DF. Anais... Brasília, DF: Sociedade Brasileira de Fitopatologia, 2023. p. 124.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
|  |
| 70. | | GUIMARÃES JUNIOR, J. B.; ARAÚJO, B. L. M.; LOPES, O. P.; MENDES, R. F.; MENDES, L. M. Produção de painéis aglomerados da madeira de desrama de Acacia mangium. Pesquisa Florestal Brasileira, Colombo, v. 33, n. 76, p. 387-391, out./dez. 2013.| Biblioteca(s): Embrapa Florestas. |
| |
| 77. | | BUFALINO, L.; MENDES, L. M.; TONOLI, G. H. D.; COSTA, T. G.; CAIXETA, L. A.; MARCONCINI, J. M. Isolamento da celulose de madeiras amazônicas para a proução de nanofibras: análise preliminar. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 7.; ESCOLA DE NANOTECNOLOGIA, 3., 2013, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação, 2013. p. 211-213 Editores: Maria Alice Martins, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso. CD-ROM. Editores: Maria Alice Martins, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
| |
| 78. | | MESQUITA, R. G. de A.; MENDES, L. M.; MENDES, R. F.; TONOLI, G. H. D.; MARCONCINI, J. M. Inclusão de feixes de sisal na produção de painéis MDP de eucalipto. Scientia forestalis, Piracicaba, v. 43, n. 105, p. 75-82, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Instrumentação. |
| |
| 79. | | GUIMARÃES JUNIOR, J. B.; XAVIER, M. M.; SANTOS, T. S.; PROTÁSIO, T. de P.; MENDES, R. F.; MENDES, L. M. Inclusão de resíduo da cultura de sorgo em painéis aglomerados de eucalipto. Pesquisa Florestal Brasileira, Colombo, v. 36, n. 88, p. 435-442, out./dez. 2016.| Biblioteca(s): Embrapa Florestas. |
| |
| 80. | | LOPES, T. A.; BUFALINO, L.; CLARO, P. I. C.; MARTINS, M. A.; TONOLI, G. H. D.; MENDES, L. M. The effect of surface modifications with corona discharge in pinus and eucalyptus nanofibril films. Cellulose, v. 25, n. 9, p. 5017-5033, set. 2018. 5017-5033| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Instrumentação. |
| |
| Registros recuperados : 189 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
