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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
01/10/2019 |
Data da última atualização: |
12/02/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
RODRIGUES, L. L.; RODRIGUES, L. A.; SOUZA, T. L. P. O. de; MELO, L. C.; PEREIRA, H. S. |
Afiliação: |
LUDVINIA L. RODRIGUES, UFG; LUANA ALVES RODRIGUES, CNPAF; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; LEONARDO CUNHA MELO, CNPAF; HELTON SANTOS PEREIRA, CNPAF. |
Título: |
Genetic control of seed coat darkening in common bean cultivars from three market classes. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Crop Science, v. 59, n. 5, p. 2046-2054, Sep./Oct. 2019. |
DOI: |
10.2135/cropsci2019.03.0161 |
Idioma: |
Inglês |
Conteúdo: |
Common bean (Phaseolus vulgaris L.) cultivars with slow seed coat darkening benefit the growers by extending the seed storage period without losses in commercial quality. Seed coat darkening in pinto and carioca seeded common bean is controlled by recessive genes, but it is not known whether these genes are different or not. The objectives of this study were (i) to verify if the gene that controls seed coat darkening in common bean cultivars from different market classes is the same, and (ii) to evaluate the efficiency of the simple sequence repeat (SSR) marker Pvsd-1158 and the single nucleotide polymorphism (SNP) marker PvbHLHp12804 linked to the Sd gene in identifying carioca and mulatinho seeded lines contrasting for seed coat darkening. Seventeen lines were used for genotypic and phenotypic evaluations in two trials, and F2:3 progenies obtained from crosses between one pinto bean line (Sd gene) and two carioca lines that have slow seed coat darkening were used in segregation tests. The result of the two markers was identical and shown 87.5% coincidence with phenotypic data. Only two lines, CNFM 11940 (mulatinho) and TAA Dama (carioca), exhibited slow seed coat darkening and marker alleles related to regular darkening. Therefore, there was recombination between the markers and the Sd gene, or there is another gene causing slow seed coat darkening in these lines. All F2:3 progenies exhibited slow seed coat darkening, indicating that there was no segregation. The two markers are efficient for assisted selection. Results of the genotypic evaluations and the segregation test indicating that the same gene (Sd) is responsible for seed coat darkening in pinto and carioca beans. MenosCommon bean (Phaseolus vulgaris L.) cultivars with slow seed coat darkening benefit the growers by extending the seed storage period without losses in commercial quality. Seed coat darkening in pinto and carioca seeded common bean is controlled by recessive genes, but it is not known whether these genes are different or not. The objectives of this study were (i) to verify if the gene that controls seed coat darkening in common bean cultivars from different market classes is the same, and (ii) to evaluate the efficiency of the simple sequence repeat (SSR) marker Pvsd-1158 and the single nucleotide polymorphism (SNP) marker PvbHLHp12804 linked to the Sd gene in identifying carioca and mulatinho seeded lines contrasting for seed coat darkening. Seventeen lines were used for genotypic and phenotypic evaluations in two trials, and F2:3 progenies obtained from crosses between one pinto bean line (Sd gene) and two carioca lines that have slow seed coat darkening were used in segregation tests. The result of the two markers was identical and shown 87.5% coincidence with phenotypic data. Only two lines, CNFM 11940 (mulatinho) and TAA Dama (carioca), exhibited slow seed coat darkening and marker alleles related to regular darkening. Therefore, there was recombination between the markers and the Sd gene, or there is another gene causing slow seed coat darkening in these lines. All F2:3 progenies exhibited slow seed coat darkening, indicating that there was no segregation. The two marke... Mostrar Tudo |
Thesagro: |
Controle Genético; Feijão; Melhoramento Genético Vegetal; Phaseolus Vulgaris; Tratamento de Semente. |
Thesaurus Nal: |
Beans; Genetic markers; Seed coat. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02537naa a2200277 a 4500 001 2112628 005 2020-02-12 008 2019 bl uuuu u00u1 u #d 024 7 $a10.2135/cropsci2019.03.0161$2DOI 100 1 $aRODRIGUES, L. L. 245 $aGenetic control of seed coat darkening in common bean cultivars from three market classes.$h[electronic resource] 260 $c2019 520 $aCommon bean (Phaseolus vulgaris L.) cultivars with slow seed coat darkening benefit the growers by extending the seed storage period without losses in commercial quality. Seed coat darkening in pinto and carioca seeded common bean is controlled by recessive genes, but it is not known whether these genes are different or not. The objectives of this study were (i) to verify if the gene that controls seed coat darkening in common bean cultivars from different market classes is the same, and (ii) to evaluate the efficiency of the simple sequence repeat (SSR) marker Pvsd-1158 and the single nucleotide polymorphism (SNP) marker PvbHLHp12804 linked to the Sd gene in identifying carioca and mulatinho seeded lines contrasting for seed coat darkening. Seventeen lines were used for genotypic and phenotypic evaluations in two trials, and F2:3 progenies obtained from crosses between one pinto bean line (Sd gene) and two carioca lines that have slow seed coat darkening were used in segregation tests. The result of the two markers was identical and shown 87.5% coincidence with phenotypic data. Only two lines, CNFM 11940 (mulatinho) and TAA Dama (carioca), exhibited slow seed coat darkening and marker alleles related to regular darkening. Therefore, there was recombination between the markers and the Sd gene, or there is another gene causing slow seed coat darkening in these lines. All F2:3 progenies exhibited slow seed coat darkening, indicating that there was no segregation. The two markers are efficient for assisted selection. Results of the genotypic evaluations and the segregation test indicating that the same gene (Sd) is responsible for seed coat darkening in pinto and carioca beans. 650 $aBeans 650 $aGenetic markers 650 $aSeed coat 650 $aControle Genético 650 $aFeijão 650 $aMelhoramento Genético Vegetal 650 $aPhaseolus Vulgaris 650 $aTratamento de Semente 700 1 $aRODRIGUES, L. A. 700 1 $aSOUZA, T. L. P. O. de 700 1 $aMELO, L. C. 700 1 $aPEREIRA, H. S. 773 $tCrop Science$gv. 59, n. 5, p. 2046-2054, Sep./Oct. 2019.
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Embrapa Arroz e Feijão (CNPAF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Milho e Sorgo. |
Data corrente: |
10/07/2017 |
Data da última atualização: |
24/01/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
RIBEIRO, A. P.; SOUZA, W. R. de; MARTINS, P. K.; VINECKY, F.; DUARTE, K. E.; BASSO, M. F.; DIAS, B. B. A.; CAMPANHA, R. B.; OLIVEIRA, P. A. de; CENTENO, D. C.; CANÇADO, G. M. de A.; MAGALHÃES, J. V. de; SOUSA, C. A. F. de; ANDRADE, A. C.; KOBAYASHI, A. K.; MOLINARI, H. B. C. |
Afiliação: |
WAGNER R. DE SOUZA, UFLA; POLYANA K. MARTINS, UFLA; FELIPE VINECKY, UFLA; KAROLINE E. DUARTE, UFLA; MARCOS F. BASSO, UFLA; BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; RAQUEL BOMBARDA CAMPANHA, CNPAE; PATRICIA ABRAO DE OLIVEIRA, CNPAE; DANILO C. CENTENO, UFABC; GERALDO MAGELA DE ALMEIDA CANCADO, CNPTIA; JURANDIR VIEIRA DE MAGALHAES, CNPMS; CARLOS A. F. DE SOUSA, UFLA; ALAN CARVALHO ANDRADE, SAPC; ADILSON KENJI KOBAYASHI, CNPAE; HUGO BRUNO CORREA MOLINARI, CNPAE. |
Título: |
Overexpression of BdMATE gene improves aluminum tolerance in Setaria viridis. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Frontiers in Plant Science, v. 8, p. 1-12, June 2017. |
Páginas: |
12 p. |
DOI: |
10.3389/fpls.2017.00865 |
Idioma: |
Inglês |
Conteúdo: |
Acidic soils are distributed worldwide, predominantly in tropical and subtropical areas,reaching around 50% of the arable soil. This type of soil strongly reduces crop production, mainly because of the presence of aluminum, which has its solubility increased at low pH levels. A well-known physiological mechanism used by plants to cope with Al stress involves activation of membrane transporters responsible for organic acid anions secretion from the root apex to the rhizosphere, which chelate Al, preventing its absorption by roots. In sorghum, a membrane transporter gene belonging to multidrug and toxic compound extrusion (MATE) family was identified and characterized as an aluminum-activated citrate transporter gene responsible for Al tolerance in this crop. Setaria viridis is an emerging model for C4 species and it is an important model to validate some genes for further C4 crops transformation, such as sugarcane, maize, and wheat. In the present work, Setaria viridis was used as a model plant to overexpress a newly identified MATE gene from Brachypodium distachyon(BdMATE), closely related to SbMATE, for aluminum tolerance assays. Transgenic S. viridis plants overexpressing a BdMATE presented an improved Al tolerance phenotype, characterized by sustained root growth and exclusion of aluminum from the root apex in transgenic plants, as confirmed by hematoxylin assay. In addition, transgenic plants showed higher root citrate exudation into the rhizosphere, suggesting that Al tolerance improvement in these plants could be related to the chelation of the metal by the organic acid anion. These results suggest that BdMATE gene can be used to transform C4 crops of economic importance with improved aluminum tolerance. MenosAcidic soils are distributed worldwide, predominantly in tropical and subtropical areas,reaching around 50% of the arable soil. This type of soil strongly reduces crop production, mainly because of the presence of aluminum, which has its solubility increased at low pH levels. A well-known physiological mechanism used by plants to cope with Al stress involves activation of membrane transporters responsible for organic acid anions secretion from the root apex to the rhizosphere, which chelate Al, preventing its absorption by roots. In sorghum, a membrane transporter gene belonging to multidrug and toxic compound extrusion (MATE) family was identified and characterized as an aluminum-activated citrate transporter gene responsible for Al tolerance in this crop. Setaria viridis is an emerging model for C4 species and it is an important model to validate some genes for further C4 crops transformation, such as sugarcane, maize, and wheat. In the present work, Setaria viridis was used as a model plant to overexpress a newly identified MATE gene from Brachypodium distachyon(BdMATE), closely related to SbMATE, for aluminum tolerance assays. Transgenic S. viridis plants overexpressing a BdMATE presented an improved Al tolerance phenotype, characterized by sustained root growth and exclusion of aluminum from the root apex in transgenic plants, as confirmed by hematoxylin assay. In addition, transgenic plants showed higher root citrate exudation into the rhizosphere, suggesting that Al t... Mostrar Tudo |
Palavras-Chave: |
BdMate; Hydroponic system; Organismo geneticamente modificado; Tolerância ao alumínio. |
Thesagro: |
Aluminio. |
Thesaurus NAL: |
Abiotic stress; Aluminum; Genetically modified organisms; Setaria viridis. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02981naa a2200433 a 4500 001 2080632 005 2018-01-24 008 2017 bl uuuu u00u1 u #d 024 7 $a10.3389/fpls.2017.00865$2DOI 100 1 $aRIBEIRO, A. P. 245 $aOverexpression of BdMATE gene improves aluminum tolerance in Setaria viridis.$h[electronic resource] 260 $c2017 300 $a12 p. 520 $aAcidic soils are distributed worldwide, predominantly in tropical and subtropical areas,reaching around 50% of the arable soil. This type of soil strongly reduces crop production, mainly because of the presence of aluminum, which has its solubility increased at low pH levels. A well-known physiological mechanism used by plants to cope with Al stress involves activation of membrane transporters responsible for organic acid anions secretion from the root apex to the rhizosphere, which chelate Al, preventing its absorption by roots. In sorghum, a membrane transporter gene belonging to multidrug and toxic compound extrusion (MATE) family was identified and characterized as an aluminum-activated citrate transporter gene responsible for Al tolerance in this crop. Setaria viridis is an emerging model for C4 species and it is an important model to validate some genes for further C4 crops transformation, such as sugarcane, maize, and wheat. In the present work, Setaria viridis was used as a model plant to overexpress a newly identified MATE gene from Brachypodium distachyon(BdMATE), closely related to SbMATE, for aluminum tolerance assays. Transgenic S. viridis plants overexpressing a BdMATE presented an improved Al tolerance phenotype, characterized by sustained root growth and exclusion of aluminum from the root apex in transgenic plants, as confirmed by hematoxylin assay. In addition, transgenic plants showed higher root citrate exudation into the rhizosphere, suggesting that Al tolerance improvement in these plants could be related to the chelation of the metal by the organic acid anion. These results suggest that BdMATE gene can be used to transform C4 crops of economic importance with improved aluminum tolerance. 650 $aAbiotic stress 650 $aAluminum 650 $aGenetically modified organisms 650 $aSetaria viridis 650 $aAluminio 653 $aBdMate 653 $aHydroponic system 653 $aOrganismo geneticamente modificado 653 $aTolerância ao alumínio 700 1 $aSOUZA, W. R. de 700 1 $aMARTINS, P. K. 700 1 $aVINECKY, F. 700 1 $aDUARTE, K. E. 700 1 $aBASSO, M. F. 700 1 $aDIAS, B. B. A. 700 1 $aCAMPANHA, R. B. 700 1 $aOLIVEIRA, P. A. de 700 1 $aCENTENO, D. C. 700 1 $aCANÇADO, G. M. de A. 700 1 $aMAGALHÃES, J. V. de 700 1 $aSOUSA, C. A. F. de 700 1 $aANDRADE, A. C. 700 1 $aKOBAYASHI, A. K. 700 1 $aMOLINARI, H. B. C. 773 $tFrontiers in Plant Science$gv. 8, p. 1-12, June 2017.
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