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Registro Completo |
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
22/03/2016 |
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Data da última atualização: |
22/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, C. R. C.; PONTES, R. G. M. S. de; LIMA, L. M. de; MARTINS, E. S.; MELO FILHO, P. A.; PINHEIRO, M. P. N.; SANTOS, R. C. dos. |
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Afiliação: |
CARLIANE R. C. SILVA, UFRPE; ROSE GOMES MONNERAT SOLON DE PONTES, CENARGEN; LIZIANE MARIA DE LIMA, CNPA; ÉRICA S. MARTINS, CENARGEN; PÉRICLES A. MELO FIILHO, UFRPE; MORGANNA P. N. PINHEIRO, UFRPE; ROSEANE CAVALCANTI DOS SANTOS, CNPA. |
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Título: |
Stable integration and expression of a cry1 la gene conferring resistance to fall armyworm and boll weevil in cotton plants. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pest Management Science, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
BACKGROUND: Boll weevil is a serious pest of cotton crop. Effective control involves applications of chemical insecticides, increasing the cost of production and environmental pollution. The current genetically modified Bt crops have allowed great benefits to farmers but show activity limited to lepidopteran pests. This work reports on procedures adopted for integration and expression of a cry transgene conferring resistance to boll weevil and fall armyworm by usingmolecular tools. RESULTS: Four Brazilian cotton cultivars were microinjected with a minimal linear cassette generating 1248 putative lines. Complete gene integration was found in only one line (T0-34) containing one copy of cry1Ia detected by Southern blot. Protein was expressed in high concentration at 45 days after emergence (dae), decreasing by approximately 50% at 90 dae. Toxicity of the cry protein was demonstrated in feeding bioassays revealing 56.7% mortality to boll weevil fed buds and 88.1% mortality to fall armyworm fed leaves. A binding of cry1Ia antibody was found in the midgut of boll weevils fed on T0-34 buds in an immunodetection assay. CONCLUSION: The gene introduced into plants confers resistance to boll weevil and fall armyworm. Transmission of the transgene occurred normally to T1 progeny.All plants showed phenotypically normal growth,with fertile flowers and abundant seeds. |
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Palavras-Chave: |
Chemical inseticides. |
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Thesagro: |
Anthonomus grandis; Gossypium hirsutum; Spodoptera frugiperda. |
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Thesaurus Nal: |
cotton. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141527/1/Stable-integration-and-expression-of-a-cry1-...pdf
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Marc: |
LEADER 02151naa a2200253 a 4500
001 2041613
005 2016-03-22
008 2015 bl uuuu u00u1 u #d
100 1 $aSILVA, C. R. C.
245 $aStable integration and expression of a cry1 la gene conferring resistance to fall armyworm and boll weevil in cotton plants.$h[electronic resource]
260 $c2015
520 $aBACKGROUND: Boll weevil is a serious pest of cotton crop. Effective control involves applications of chemical insecticides, increasing the cost of production and environmental pollution. The current genetically modified Bt crops have allowed great benefits to farmers but show activity limited to lepidopteran pests. This work reports on procedures adopted for integration and expression of a cry transgene conferring resistance to boll weevil and fall armyworm by usingmolecular tools. RESULTS: Four Brazilian cotton cultivars were microinjected with a minimal linear cassette generating 1248 putative lines. Complete gene integration was found in only one line (T0-34) containing one copy of cry1Ia detected by Southern blot. Protein was expressed in high concentration at 45 days after emergence (dae), decreasing by approximately 50% at 90 dae. Toxicity of the cry protein was demonstrated in feeding bioassays revealing 56.7% mortality to boll weevil fed buds and 88.1% mortality to fall armyworm fed leaves. A binding of cry1Ia antibody was found in the midgut of boll weevils fed on T0-34 buds in an immunodetection assay. CONCLUSION: The gene introduced into plants confers resistance to boll weevil and fall armyworm. Transmission of the transgene occurred normally to T1 progeny.All plants showed phenotypically normal growth,with fertile flowers and abundant seeds.
650 $acotton
650 $aAnthonomus grandis
650 $aGossypium hirsutum
650 $aSpodoptera frugiperda
653 $aChemical inseticides
700 1 $aPONTES, R. G. M. S. de
700 1 $aLIMA, L. M. de
700 1 $aMARTINS, E. S.
700 1 $aMELO FILHO, P. A.
700 1 $aPINHEIRO, M. P. N.
700 1 $aSANTOS, R. C. dos
773 $tPest Management Science, 2015.
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Registro original: |
Embrapa Algodão (CNPA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
04/03/2011 |
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Data da última atualização: |
17/10/2023 |
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Tipo da produção científica: |
Orientação de Tese de Pós-Graduação |
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Autoria: |
XAVIER, L. M. S. |
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Afiliação: |
LUCIANE MODENEZ SALDIVAR XAVIER. |
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Título: |
Ecologia química de Elasmopalpus lignosellus (Zeller): comunicação química durante o comportamento reprodutivo e a interação tritrófica envolvendo milho e o parasitóide Trichogramma pretiosum Riley. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
2010. |
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Páginas: |
127 f. |
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Idioma: |
Português |
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Notas: |
Tese (Doutorado) - Universidade Federal de Viçosa, Viçosa, MG. Co-orientador: Paulo Afonso Viana. |
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Palavras-Chave: |
Ecologia química. |
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Thesagro: |
Inseto; Praga. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00647nam a2200157 a 4500
001 1880032
005 2023-10-17
008 2010 bl uuuu m 00u1 u #d
100 1 $aXAVIER, L. M. S.
245 $aEcologia química de Elasmopalpus lignosellus (Zeller)$bcomunicação química durante o comportamento reprodutivo e a interação tritrófica envolvendo milho e o parasitóide Trichogramma pretiosum Riley.$h[electronic resource]
260 $a2010.$c2010
300 $a127 f.
500 $aTese (Doutorado) - Universidade Federal de Viçosa, Viçosa, MG. Co-orientador: Paulo Afonso Viana.
650 $aInseto
650 $aPraga
653 $aEcologia química
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Embrapa Milho e Sorgo (CNPMS) |
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