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| Registros recuperados : 13 | |
| 6. |  | PEREIRA, N. C.; MELO, V. S. de; HUNGRIA, L. C. da; BRASIL, E. C. Atributos físicos de solos submetidos a diferentes sistemas de uso da terra no município de Paragominas. In: SEMINÁRIO ANUAL DE INICIAÇÃO CIENTÍFICA DA UFRA, 15., 2017, Belém, PA. Anais... Belém, PA: UFRA, 2018. p. 70. Organizadoras: Paula Fernanda Viegas Pinheiro e Tatianne Feitosa Soares.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 8. | | SILVA JUNIOR, M. L. da; DESJARDINS, T.; SARRAZIN, M.; MELO, V. S. de; MARTINS, P. F. da S.; SANTOS, E. R.; CARVALHO, C. J. R. de. Carbon content in amazonian oxisols after forest conversion to pasture. Revista Brasileira de Ciência do Solo, Viçosa, MG, v. 33, n. 6, p. 1603-1611, Nov./Dec. 2009.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 9. | | PEDROSO, A. J. S.; SILVA JUNIOR, M. L. da; MELO, V. S. de; LEAL, N. C.; MELO JUNIOR, J. A.; VASCONCELOS, J. M.; TEIXEIRA, O. M. M. Influência das omissões de macronutrientes no crescimento e desenvolvimento do sistema radicular e foliar de mudas de Chamma (mentha piperita L.). In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 29.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 13.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 11.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 8., 2010, Guarapari. Fontes de nutrientes e produção agrícola: modelando o futuro: anais. Viçosa, MG: SBCS, 2010. Fertbio 2010.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 10. | | MELO, V. S. de; MARQUES, J. D.; SILVA JÚNIOR, M. L. da; MEYER, L. F. F.; DIAS, V. H. R.; AGUIAR, T. de S.; TEIXEIRA, O. M. M. Atributos físicos e químicos de solos sob diferentes tipos de uso e manejo no assentamento Abril Vermelho, Amazônia Oriental. Conjecturas, v. 22, n. 1, p. 79-97, 2021.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 11. | | SILVA JUNIOR, M. L. da; SOUZA JUNIOR, J. C. de; BRAGA, A. C. M.; OHASHI, O. S.; MELO, V. S. de; SILVA, G. R. da; PEDROSO, A. J. S.; VIÉGAS, I. de J. M.; SALDANHA, E. C. M. Crescimento de mogno-brasileiro e resistência a Hypsipyla grandella em função do cálcio e do boro. Revista Árvore, Viçosa, MG, v. 38, n. 6, p. 1085-1094, 2014.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 12. | | PEDROSO, A. J. S.; SILVA JÚNIOR, M. L. da; SILVA, G. R. da; OHASHI, O. S.; BIRANI, S. M.; LEAL, N. C.; MELO, V. S. de; TEIXEIRA, O. M. M.; LOBATO, A. K. da S.; OLIVEIRA NETO, C. F. de; SILVA, R. T. L. da; RUIVO, M. de L. P. Influence of liming and boron on development of young Swietenia macrophylla plants grown in Yellow Oxisol. Journal of Food, Agriculture and Environment, v. 10, n. 2, p. 1257-1261, 2012.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 13. | | SILVA, D. A. S.; SILVA JÚNIOR, M. L. da; VIÉGAS, I. de J. M.; LOBATO, A. K. da S.; MELO, V. S. de; BOTELHO, S. M. A.; SILVA, G. R. da; FREITAS, J. M. N. de; OLIVEIRA NETO, C. F. de; CUNHA, M. L. A. da; ARAUJO, A. R. da R. Growth and visual symptoms of nutrient deficiencies in young Mentha piperita plants. Journal of Food, Agriculture & Environment, v. 12, n. 3/4, p. 292-296, 2014.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 13 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
22/02/2013 |
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Data da última atualização: |
07/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
MACHADO, G. M.; CAIXETA, E. S.; LUCCI, C. M.; RUMPF, R.; FRANCO, M. M.; DODE, M. A. N. |
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Afiliação: |
GRAZIELI MARINHEIRO MACHADO, UnB; ESTER SIQUEIRA CAIXETA, UnB; CAROLINA MADEIRA LUCCI, UnB; RODOLFO RUMPF, CENARGEN; MAURICIO MACHAIM FRANCO, CENARGEN; MARGOT ALVES NUNES DODE, CENARGEN. |
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Título: |
Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Zygote, v. 20, p. 123-134, 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demonstrated similar development but different gene expression. MenosThe objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demon... Mostrar Tudo |
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Palavras-Chave: |
Cultura do embrião; Sexagem. |
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Thesagro: |
Gado. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179338/1/posthatching-development-of-bovine-embryos-in-vitro-the-effects-of-tunnel-preparation-and-gender.pdf
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Marc: |
LEADER 02172naa a2200217 a 4500
001 1950734
005 2023-03-07
008 2011 bl uuuu u00u1 u #d
100 1 $aMACHADO, G. M.
245 $aPost-hatching development of bovine embryos in vitro$bthe effects of tunnel preparation and gender.$h[electronic resource]
260 $c2011
520 $aThe objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demonstrated similar development but different gene expression.
650 $aGado
653 $aCultura do embrião
653 $aSexagem
700 1 $aCAIXETA, E. S.
700 1 $aLUCCI, C. M.
700 1 $aRUMPF, R.
700 1 $aFRANCO, M. M.
700 1 $aDODE, M. A. N.
773 $tZygote$gv. 20, p. 123-134, 2011.
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