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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
06/08/2019 |
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Data da última atualização: |
12/02/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALVARES, R. C.; STONEHOUSE, R.; SOUZA, T. L. P. O.; MELO, P. G. S.; MIKLAS, P. N.; BETT, K. E.; MELO, L. C.; RODRIGUES, L. A.; SOUZA, L. L.; PEREIRA, H. S. |
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Afiliação: |
RENATA C. ALVARES, UFG; ROBERT STONEHOUSE, UNIVERSITY OF SASKATCHEWAN, Canada; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; PATRICIA G. S. MELO, UFG; PHILLIP N. MIKLAS, USDA, Prosser-WA; KIRSTIN E. BETT, UNIVERSITY OF SASKATCHEWAN, Canada; LEONARDO CUNHA MELO, CNPAF; LUANA ALVES RODRIGUES, CNPAF; LORENA L. SOUZA, UFG; HELTON SANTOS PEREIRA, CNPAF. |
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Título: |
Generation and validation of genetic markers for the selection of carioca dry bean genotypes with the slow-darkening seed coat trait. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Euphytica, v. 215, n. 8, Aug. 2019. |
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ISSN: |
1573-5060 |
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DOI: |
10.1007/s10681-019-2461-y |
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Idioma: |
Inglês |
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Conteúdo: |
Slow darkening (SD) is a trait that helps to maintain a brighter seed coat appearance in certain market classes of dry beans. The aim of this study was to generate new fluorescence-based markers and validate previously identified microsatellite markers for linkage to the SD trait in lines of the carioca market class. Four segregating populations were generated by Embrapa, the Brazilian Agricultural Research Corporation, from crosses between the SD cultivar BRSMG Madrepérola and the regular-darkening cultivars BRS Estilo, BRS Cometa, BRS Notável and BRS Sublime. These populations were screened with the simple-sequence markers Pvsd-1158 and PVM02TC116 and with a TaqMan marker designed for the single-nucleotide polymorphism (SNP) PvbHLHp12804. A KASP marker was also designed for the PvbHLHp12804 marker for testing on advanced carioca lines developed by the University of Saskatchewan. In the carioca lines developed by Embrapa, PVM02TC116 proved unsuitable for marker-assisted selection (MAS). Both the Pvsd-1158 and PvbHLHp12804 markers were found to be tightly linked to the gene responsible for the SD trait, with genetic distances calculated at 2.8 cM for Pvsd-1158 and 2.0 and 3.1 cM for PvbbHLHp12804, respectively. These markers presented more than 97% of selection efficiency. The genotypic scoring using the PvbHLHp12804 KASP marker was perfectly correlated with the phenotype in all lines of the University of Saskatchewan. The results of this study validates the use of Pvsd-1158 as a gel-based marker for SD in carioca beans. The new fluorescence-based SNP PvbHLHp12804 markers exhibited very tight linkage to SD in carioca and pinto bean lines. These markers will be ideal for MAS for the SD trait in these market classes. MenosSlow darkening (SD) is a trait that helps to maintain a brighter seed coat appearance in certain market classes of dry beans. The aim of this study was to generate new fluorescence-based markers and validate previously identified microsatellite markers for linkage to the SD trait in lines of the carioca market class. Four segregating populations were generated by Embrapa, the Brazilian Agricultural Research Corporation, from crosses between the SD cultivar BRSMG Madrepérola and the regular-darkening cultivars BRS Estilo, BRS Cometa, BRS Notável and BRS Sublime. These populations were screened with the simple-sequence markers Pvsd-1158 and PVM02TC116 and with a TaqMan marker designed for the single-nucleotide polymorphism (SNP) PvbHLHp12804. A KASP marker was also designed for the PvbHLHp12804 marker for testing on advanced carioca lines developed by the University of Saskatchewan. In the carioca lines developed by Embrapa, PVM02TC116 proved unsuitable for marker-assisted selection (MAS). Both the Pvsd-1158 and PvbHLHp12804 markers were found to be tightly linked to the gene responsible for the SD trait, with genetic distances calculated at 2.8 cM for Pvsd-1158 and 2.0 and 3.1 cM for PvbbHLHp12804, respectively. These markers presented more than 97% of selection efficiency. The genotypic scoring using the PvbHLHp12804 KASP marker was perfectly correlated with the phenotype in all lines of the University of Saskatchewan. The results of this study validates the use of Pvsd-1158... Mostrar Tudo |
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Palavras-Chave: |
SNP; SSR. |
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Thesagro: |
Feijão; Genótipo; Marcador Molecular; Melhoramento Genético Vegetal; Phaseolus Vulgaris. |
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Thesaurus Nal: |
Beans; Marker-assisted selection. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02782naa a2200361 a 4500
001 2111158
005 2020-02-12
008 2019 bl uuuu u00u1 u #d
022 $a1573-5060
024 7 $a10.1007/s10681-019-2461-y$2DOI
100 1 $aALVARES, R. C.
245 $aGeneration and validation of genetic markers for the selection of carioca dry bean genotypes with the slow-darkening seed coat trait.$h[electronic resource]
260 $c2019
520 $aSlow darkening (SD) is a trait that helps to maintain a brighter seed coat appearance in certain market classes of dry beans. The aim of this study was to generate new fluorescence-based markers and validate previously identified microsatellite markers for linkage to the SD trait in lines of the carioca market class. Four segregating populations were generated by Embrapa, the Brazilian Agricultural Research Corporation, from crosses between the SD cultivar BRSMG Madrepérola and the regular-darkening cultivars BRS Estilo, BRS Cometa, BRS Notável and BRS Sublime. These populations were screened with the simple-sequence markers Pvsd-1158 and PVM02TC116 and with a TaqMan marker designed for the single-nucleotide polymorphism (SNP) PvbHLHp12804. A KASP marker was also designed for the PvbHLHp12804 marker for testing on advanced carioca lines developed by the University of Saskatchewan. In the carioca lines developed by Embrapa, PVM02TC116 proved unsuitable for marker-assisted selection (MAS). Both the Pvsd-1158 and PvbHLHp12804 markers were found to be tightly linked to the gene responsible for the SD trait, with genetic distances calculated at 2.8 cM for Pvsd-1158 and 2.0 and 3.1 cM for PvbbHLHp12804, respectively. These markers presented more than 97% of selection efficiency. The genotypic scoring using the PvbHLHp12804 KASP marker was perfectly correlated with the phenotype in all lines of the University of Saskatchewan. The results of this study validates the use of Pvsd-1158 as a gel-based marker for SD in carioca beans. The new fluorescence-based SNP PvbHLHp12804 markers exhibited very tight linkage to SD in carioca and pinto bean lines. These markers will be ideal for MAS for the SD trait in these market classes.
650 $aBeans
650 $aMarker-assisted selection
650 $aFeijão
650 $aGenótipo
650 $aMarcador Molecular
650 $aMelhoramento Genético Vegetal
650 $aPhaseolus Vulgaris
653 $aSNP
653 $aSSR
700 1 $aSTONEHOUSE, R.
700 1 $aSOUZA, T. L. P. O.
700 1 $aMELO, P. G. S.
700 1 $aMIKLAS, P. N.
700 1 $aBETT, K. E.
700 1 $aMELO, L. C.
700 1 $aRODRIGUES, L. A.
700 1 $aSOUZA, L. L.
700 1 $aPEREIRA, H. S.
773 $tEuphytica$gv. 215, n. 8, Aug. 2019.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca |
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| Registros recuperados : 176 | |
| 5. |  | PICHELLI, K. R.; MELO, P. G. S. de; JAQUES, L. C. Ações de comunicação para desmistificar conceitos e incentivar o conhecimento sobre a araucária. Pesquisa Florestal Brasileira, Colombo, v. 39, (nesp), e201902043, 2019. p. 485. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 6. | | MELO, P. G. S. de; PICHELLI, K. R.; JAQUES, L. C. A ciência por trás dos alimentos: produção de materiais para participação da Embrapa Florestas na Semana Sabores do Pinhão. Pesquisa Florestal Brasileira, Colombo, v. 39, (nesp), e201902043, 2019. p. 485. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 7. | | PEREIRA, A. F.; ASSUNÇÃO, A.; OLIVEIRA, J. P.; XIMENES, P. A.; MELO, P. G. S. Caracterização agronômica e nutricional na avaliação de genótipos de milho doce. In: CONGRESSO DE PESQUISA, ENSINO E EXTENSÃO, 4., 2007, Goiânia. Ciência, educação e compromisso social: anais... Goiânia: Universidade Federal de Goiás, 2007. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 8. | | PICHELLI, K. R.; JAQUES, L. C.; MELO, P. G. S. de. Comunicação como estratégia para sensibilização do público urbano para as questões florestais. Pesquisa Florestal Brasileira, Colombo, v. 39, (nesp), e201902043, 2019. p. 699. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 18. | | FARIA, L. C. de; MELO, L. C.; DEL PELOSO, M. J.; MELO, P. G. S. Ganho genético do programa de melhoramento do feijoeiro comum da Embrapa no Brasil. In: CONGRESSO DE PESQUISA, ENSINO E EXTENSÃO, 4., 2007, Goiânia. Ciência, educação e compromisso social: anais... Goiânia: Universidade Federal de Goiás, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 176 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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