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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
29/12/2020 |
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Data da última atualização: |
29/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, L. J. da; CREVELIN, E. J.; SOUZA, D. T.; LACERDA JÚNIOR, G. V.; OLIVEIRA, V. M.; RUIZ, A. L. T. G.; ROSA, L. H.; MORAES, L. A. B.; MELO, I. S. de. |
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Afiliação: |
LEONARDO JOSÉ SILVA, ESALQ-USP; EDUARDO JOSÉ CREVELIN, FFCLRP-USP; DANILO TOSTA SOUZA, FFCLRP-USP; GILENO VIEIRA LACERDA JÚNIOR; VALERIA MAIA DE OLIVEIRA, CPQBA-UNICAMP; ANA LUCIA TASCA GOIS RUIZ, FCF-UNICAMP; LUIZ HENRIQUE ROSA, UFMG; LUIZ ALBERTO BERALDO MORAES, FFCLRP-USP; ITAMAR SOARES DE MELO, CNPMA. |
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Título: |
Actinobacteria from Antarctica as a source for anticancer discovery. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Scientific Reports, v. 10, article 13870, 2020. |
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Páginas: |
p. 1-15. |
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ISSN: |
2045-2322 |
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DOI: |
https://doi.org/10.1038/s41598-020-69786-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Although many advances have been achieved to treat aggressive tumours, cancer remains a leading cause of death and a public health problem worldwide. Among the main approaches for the discovery of new bioactive agents, the prospect of microbial secondary metabolites represents an effective source for the development of drug leads. In this study, we investigated the actinobacterial diversity associated with an endemic Antarctic species, Deschampsia antarctica, by integrated culture-dependent and culture-independent methods and acknowledged this niche as a reservoir of bioactive strains for the production of antitumour compounds. The 16S rRNA-based analysis showed the predominance of the Actinomycetales order, a well-known group of bioactive metabolite producers belonging to the Actinobacteria phylum. Cultivation techniques were applied, and 72 psychrotolerant Actinobacteria strains belonging to the genera Actinoplanes, Arthrobacter, Kribbella, Mycobacterium, Nocardia, Pilimelia, Pseudarthrobacter, Rhodococcus, Streptacidiphilus, Streptomyces and Tsukamurella were identified. The secondary metabolites were screened, and 17 isolates were identified as promising antitumour compound producers. However, the bio-guided assay showed a pronounced antiproliferative activity for the crude extracts of Streptomyces sp. CMAA 1527 and Streptomyces sp. CMAA 1653. The TGI and LC50 values revealed the potential of these natural products to control the proliferation of breast (MCF-7), glioblastoma (U251), lung/non-small (NCI-H460) and kidney (786-0) human cancer cell lines. Cinerubin B and actinomycin V were the predominant compounds identified in Streptomyces sp. CMAA 1527 and Streptomyces sp. CMAA 1653, respectively. Our results suggest that the rhizosphere of D. antarctica represents a prominent reservoir of bioactive actinobacteria strains and reveals it as an important environment for potential antitumour agents. MenosAbstract: Although many advances have been achieved to treat aggressive tumours, cancer remains a leading cause of death and a public health problem worldwide. Among the main approaches for the discovery of new bioactive agents, the prospect of microbial secondary metabolites represents an effective source for the development of drug leads. In this study, we investigated the actinobacterial diversity associated with an endemic Antarctic species, Deschampsia antarctica, by integrated culture-dependent and culture-independent methods and acknowledged this niche as a reservoir of bioactive strains for the production of antitumour compounds. The 16S rRNA-based analysis showed the predominance of the Actinomycetales order, a well-known group of bioactive metabolite producers belonging to the Actinobacteria phylum. Cultivation techniques were applied, and 72 psychrotolerant Actinobacteria strains belonging to the genera Actinoplanes, Arthrobacter, Kribbella, Mycobacterium, Nocardia, Pilimelia, Pseudarthrobacter, Rhodococcus, Streptacidiphilus, Streptomyces and Tsukamurella were identified. The secondary metabolites were screened, and 17 isolates were identified as promising antitumour compound producers. However, the bio-guided assay showed a pronounced antiproliferative activity for the crude extracts of Streptomyces sp. CMAA 1527 and Streptomyces sp. CMAA 1653. The TGI and LC50 values revealed the potential of these natural products to control the proliferation of breast (MCF-7)... Mostrar Tudo |
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Palavras-Chave: |
Anticancer activity; Antitumoral activity. |
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Thesagro: |
Bactéria não Patogênica; Câncer. |
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Thesaurus Nal: |
Actinobacteria; Antarctica. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219757/1/Melo-Actinobacteria-Antarctica-2020.pdf
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Marc: |
LEADER 02877naa a2200325 a 4500
001 2128824
005 2020-12-29
008 2020 bl uuuu u00u1 u #d
022 $a2045-2322
024 7 $ahttps://doi.org/10.1038/s41598-020-69786-2$2DOI
100 1 $aSILVA, L. J. da
245 $aActinobacteria from Antarctica as a source for anticancer discovery.$h[electronic resource]
260 $c2020
300 $ap. 1-15.
520 $aAbstract: Although many advances have been achieved to treat aggressive tumours, cancer remains a leading cause of death and a public health problem worldwide. Among the main approaches for the discovery of new bioactive agents, the prospect of microbial secondary metabolites represents an effective source for the development of drug leads. In this study, we investigated the actinobacterial diversity associated with an endemic Antarctic species, Deschampsia antarctica, by integrated culture-dependent and culture-independent methods and acknowledged this niche as a reservoir of bioactive strains for the production of antitumour compounds. The 16S rRNA-based analysis showed the predominance of the Actinomycetales order, a well-known group of bioactive metabolite producers belonging to the Actinobacteria phylum. Cultivation techniques were applied, and 72 psychrotolerant Actinobacteria strains belonging to the genera Actinoplanes, Arthrobacter, Kribbella, Mycobacterium, Nocardia, Pilimelia, Pseudarthrobacter, Rhodococcus, Streptacidiphilus, Streptomyces and Tsukamurella were identified. The secondary metabolites were screened, and 17 isolates were identified as promising antitumour compound producers. However, the bio-guided assay showed a pronounced antiproliferative activity for the crude extracts of Streptomyces sp. CMAA 1527 and Streptomyces sp. CMAA 1653. The TGI and LC50 values revealed the potential of these natural products to control the proliferation of breast (MCF-7), glioblastoma (U251), lung/non-small (NCI-H460) and kidney (786-0) human cancer cell lines. Cinerubin B and actinomycin V were the predominant compounds identified in Streptomyces sp. CMAA 1527 and Streptomyces sp. CMAA 1653, respectively. Our results suggest that the rhizosphere of D. antarctica represents a prominent reservoir of bioactive actinobacteria strains and reveals it as an important environment for potential antitumour agents.
650 $aActinobacteria
650 $aAntarctica
650 $aBactéria não Patogênica
650 $aCâncer
653 $aAnticancer activity
653 $aAntitumoral activity
700 1 $aCREVELIN, E. J.
700 1 $aSOUZA, D. T.
700 1 $aLACERDA JÚNIOR, G. V.
700 1 $aOLIVEIRA, V. M.
700 1 $aRUIZ, A. L. T. G.
700 1 $aROSA, L. H.
700 1 $aMORAES, L. A. B.
700 1 $aMELO, I. S. de
773 $tScientific Reports$gv. 10, article 13870, 2020.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Biblioteca |
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Origem |
Tipo/Formato |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
22/10/2015 |
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Data da última atualização: |
26/02/2016 |
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Autoria: |
JUIZO, C. G. F.; LAIOLA, P. L.; ZEN, L. R.; MARCHESAN, R.; CARVALHO, D. E.; BILA, N. F.; EGAS, A. F.; ROCHA, M. P. da; KILTZKE, R. J. |
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Título: |
Variação radial das propriedades físicas da madeira de Pinus patula plantados em Moçambique. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Florestal Brasileira, Colombo, v. 35, n. 83, p. 285-292, jul./set. 2015. |
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DOI: |
10.4336/2015.pfb.35.83.771 |
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Idioma: |
Português |
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Conteúdo: |
Este trabalho teve por objetivo avaliar as propriedades físicas da madeira de Pinus patula, com 38 anos, oriundos de um povoamento florestal de Moçambique, localizado no município de Machipanda. A determinação das propriedades físicas como a massa especifica básica, retratibilidade e umidade da madeira, no sentido medula- casca, seguiram as recomendações da Norma Panamericana - COPANT. Os resultados evidenciaram diferença estatística, ao nível de 5% de significância, no sentido radial para o coeficiente de retratibilidade. Os valores de retratibilidade foram de 6,06 e 8,80% no lenho juvenil e 6,18 e 8,65% no lenho adulto no sentido radial e tangencial, respectivamente, com coeficiente de anisotropia de 1,43 no lenho juvenil e 2,44 no lenho adulto. A madeira de Pinus patula, plantado na floresta de Inhamacari, apresenta uma madeira estável aos 38 anos de idade, que pode ser aplicada para fins estruturais e para a fabricação de artigos de carpintaria e móveis. |
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Palavras-Chave: |
Espécie exótica; Massa específica; Qualidade da madeira; Retratibilidade; Retratibility. |
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Thesagro: |
Pinus Patula; Umidade. |
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Thesaurus NAL: |
humidity; specific gravity; wood quality. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/132788/1/VariacaoRadial.pdf
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Marc: |
LEADER 02008naa a2200349 a 4500
001 2027071
005 2016-02-26
008 2015 bl uuuu u00u1 u #d
024 7 $a10.4336/2015.pfb.35.83.771$2DOI
100 1 $aJUIZO, C. G. F.
245 $aVariação radial das propriedades físicas da madeira de Pinus patula plantados em Moçambique.$h[electronic resource]
260 $c2015
520 $aEste trabalho teve por objetivo avaliar as propriedades físicas da madeira de Pinus patula, com 38 anos, oriundos de um povoamento florestal de Moçambique, localizado no município de Machipanda. A determinação das propriedades físicas como a massa especifica básica, retratibilidade e umidade da madeira, no sentido medula- casca, seguiram as recomendações da Norma Panamericana - COPANT. Os resultados evidenciaram diferença estatística, ao nível de 5% de significância, no sentido radial para o coeficiente de retratibilidade. Os valores de retratibilidade foram de 6,06 e 8,80% no lenho juvenil e 6,18 e 8,65% no lenho adulto no sentido radial e tangencial, respectivamente, com coeficiente de anisotropia de 1,43 no lenho juvenil e 2,44 no lenho adulto. A madeira de Pinus patula, plantado na floresta de Inhamacari, apresenta uma madeira estável aos 38 anos de idade, que pode ser aplicada para fins estruturais e para a fabricação de artigos de carpintaria e móveis.
650 $ahumidity
650 $aspecific gravity
650 $awood quality
650 $aPinus Patula
650 $aUmidade
653 $aEspécie exótica
653 $aMassa específica
653 $aQualidade da madeira
653 $aRetratibilidade
653 $aRetratibility
700 1 $aLAIOLA, P. L.
700 1 $aZEN, L. R.
700 1 $aMARCHESAN, R.
700 1 $aCARVALHO, D. E.
700 1 $aBILA, N. F.
700 1 $aEGAS, A. F.
700 1 $aROCHA, M. P. da
700 1 $aKILTZKE, R. J.
773 $tPesquisa Florestal Brasileira, Colombo$gv. 35, n. 83, p. 285-292, jul./set. 2015.
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Registro original: |
Embrapa Florestas (CNPF) |
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