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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
23/08/2012 |
Data da última atualização: |
22/02/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; LIMA, M. R. de; MELO, D. S. de; NICIURA, S. C. M.; GARCIA, J. M. |
Afiliação: |
NAIARA ZOCCAL SARAIVA, Universidade Estadual Paulista; CLARA SLADE OLIVEIRA, UNESP; TATIANE ALMEIDA DRUMMOND TETZNER, UNESP; MARINA RAGAGNIN DE LIMA, UNESP/JABOTICABAL; DANILAS SALINET DE MELO, UNESP/JABOTICABAL; SIMONE CRISTINA MEO NICIURA, CPPSE; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL. |
Título: |
Chemically assisted enucleation results in higher G6PD expression in early bovine female embryos obtained by somatic cell nuclear transfer. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Cellular Reprogramming, v. 14, n. 5, p. 1-11, 2012. |
DOI: |
10.1089/cell.2011.0077 |
Idioma: |
Inglês |
Conteúdo: |
Despite extensive efforts, low efficiency is still an issue in bovine somatic cell nuclear transfer (SCNT). The hypothesis of our study was that the use of cytoplasts produced by chemically assisted enucleation (EN) would improve nuclear reprogramming in nuclear transfer (NT)?derived embryos because it results in lower damage and higher cytoplasm content than conventional EN. For that purpose, we investigated the expression of two X-linked genes: X inactive-specific transcript (XIST) and glucose 6-phosphate dehydrogenase (G6PD). In the first experiment, gene expression was assessed in day-7 female blastocysts from embryonic cell NT (ECNT) groups [conventional, ECNT conv; chemically assisted, ECNT deme (demecolcine)]. Where as in the ECNT conv group, only one embryo (25%; n = 4) expressed XIST transcripts, most embryos showed XIST expression (75%; n = 4) in the ECNT deme group. However, no significant differences in transcript abundance of XIST and G6PD were found when comparing the embryos from all groups. In a second experiment using somatic cells as nuclear donors, we evaluated gene expression profiles in female SCNT-derived embryos. No significant differences in relative abundance (RA) of XIST transcripts were observed among the groups. Nonetheless, higher ( p < 0.05) levels of G6PD were observed in SCNT deme and in vitro?derived groups in comparison to SCNT conv. To know whether higher G6PD expression in embryos derived from SCNT chemically assisted EN indicates higher metabolism in embryos considered of superior quality or if the presence of higher reactive oxygen species (ROS) levels generated by the increased oxygen consumption triggers G6PD activation, the expression of genes related to stress response should be investigated in embryos produced by that technique. MenosDespite extensive efforts, low efficiency is still an issue in bovine somatic cell nuclear transfer (SCNT). The hypothesis of our study was that the use of cytoplasts produced by chemically assisted enucleation (EN) would improve nuclear reprogramming in nuclear transfer (NT)?derived embryos because it results in lower damage and higher cytoplasm content than conventional EN. For that purpose, we investigated the expression of two X-linked genes: X inactive-specific transcript (XIST) and glucose 6-phosphate dehydrogenase (G6PD). In the first experiment, gene expression was assessed in day-7 female blastocysts from embryonic cell NT (ECNT) groups [conventional, ECNT conv; chemically assisted, ECNT deme (demecolcine)]. Where as in the ECNT conv group, only one embryo (25%; n = 4) expressed XIST transcripts, most embryos showed XIST expression (75%; n = 4) in the ECNT deme group. However, no significant differences in transcript abundance of XIST and G6PD were found when comparing the embryos from all groups. In a second experiment using somatic cells as nuclear donors, we evaluated gene expression profiles in female SCNT-derived embryos. No significant differences in relative abundance (RA) of XIST transcripts were observed among the groups. Nonetheless, higher ( p < 0.05) levels of G6PD were observed in SCNT deme and in vitro?derived groups in comparison to SCNT conv. To know whether higher G6PD expression in embryos derived from SCNT chemically assisted EN indicates higher m... Mostrar Tudo |
Palavras-Chave: |
Higher G6PD; Nuclear transfer; Somatic cell. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02567naa a2200241 a 4500 001 1932049 005 2023-02-22 008 2012 bl uuuu u00u1 u #d 024 7 $a10.1089/cell.2011.0077$2DOI 100 1 $aSARAIVA, N. Z. 245 $aChemically assisted enucleation results in higher G6PD expression in early bovine female embryos obtained by somatic cell nuclear transfer.$h[electronic resource] 260 $c2012 520 $aDespite extensive efforts, low efficiency is still an issue in bovine somatic cell nuclear transfer (SCNT). The hypothesis of our study was that the use of cytoplasts produced by chemically assisted enucleation (EN) would improve nuclear reprogramming in nuclear transfer (NT)?derived embryos because it results in lower damage and higher cytoplasm content than conventional EN. For that purpose, we investigated the expression of two X-linked genes: X inactive-specific transcript (XIST) and glucose 6-phosphate dehydrogenase (G6PD). In the first experiment, gene expression was assessed in day-7 female blastocysts from embryonic cell NT (ECNT) groups [conventional, ECNT conv; chemically assisted, ECNT deme (demecolcine)]. Where as in the ECNT conv group, only one embryo (25%; n = 4) expressed XIST transcripts, most embryos showed XIST expression (75%; n = 4) in the ECNT deme group. However, no significant differences in transcript abundance of XIST and G6PD were found when comparing the embryos from all groups. In a second experiment using somatic cells as nuclear donors, we evaluated gene expression profiles in female SCNT-derived embryos. No significant differences in relative abundance (RA) of XIST transcripts were observed among the groups. Nonetheless, higher ( p < 0.05) levels of G6PD were observed in SCNT deme and in vitro?derived groups in comparison to SCNT conv. To know whether higher G6PD expression in embryos derived from SCNT chemically assisted EN indicates higher metabolism in embryos considered of superior quality or if the presence of higher reactive oxygen species (ROS) levels generated by the increased oxygen consumption triggers G6PD activation, the expression of genes related to stress response should be investigated in embryos produced by that technique. 653 $aHigher G6PD 653 $aNuclear transfer 653 $aSomatic cell 700 1 $aOLIVEIRA, C. S. 700 1 $aTETZNER, T. A. D. 700 1 $aLIMA, M. R. de 700 1 $aMELO, D. S. de 700 1 $aNICIURA, S. C. M. 700 1 $aGARCIA, J. M. 773 $tCellular Reprogramming$gv. 14, n. 5, p. 1-11, 2012.
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Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
Biblioteca(s): |
Embrapa Acre. |
Data corrente: |
09/12/1997 |
Data da última atualização: |
13/03/2023 |
Autoria: |
MOURA, G. de M. |
Afiliação: |
GERALDO DE MELO MOURA, CPAF-AC. |
Título: |
Avaliação de cultivares e linhagens precoces de soja na microrregião do Alto Purus, Acre. |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
Rio Branco, AC: Embrapa CPAF-AC, 1997. |
Páginas: |
2 p. |
Série: |
(Embrapa CPAF-AC. Pesquisa em andamento, 113). |
ISSN: |
0101-6075 |
Idioma: |
Português |
Conteúdo: |
O presente trabalho vem sendo conduzido pela Embrapa Acre com o objetivo de avaliar o comportamento de cultivares e linhagens de soja e identificar genotipos altamente produtivos na microrregião do Alto Purus, Acre. |
Palavras-Chave: |
Aclimatación; Acre; Alto Purus; Alto Purus (AC); Amazonia Occidental; Amazônia Ocidental; Avaliação; Behaviour; Brasil; Comportamento; Cultivar; Ensayos de variedades; Evaluation; Factores ambientales; Fitomejoramiento; Linhagem precoce; Rendimiento de los cultivos; Semillas de soja; Soybean; Western Amazon. |
Thesagro: |
Aclimatação; Características Agronômicas; Comportamento de Variedade; Condição Ambiental; Glycine Max; Linhagem; Melhoramento; Melhoramento Genético Vegetal; Phaseolus Vulgaris; Produtividade; Progênie; Rendimento; Seleção; Soja; Variedade. |
Thesaurus NAL: |
Acclimation; Agronomic traits; Brazil; breeding; Crop yield; Environmental factors; Plant breeding; progeny; soybeans; varieties; Variety trials. |
Categoria do assunto: |
-- G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPAF-AC/1322/1/pesquisa113.pdf
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Marc: |
LEADER 02061nam a2200697 a 4500 001 1492597 005 2023-03-13 008 1997 bl uuuu u0uu1 u #d 022 $a0101-6075 100 1 $aMOURA, G. de M. 245 $aAvaliação de cultivares e linhagens precoces de soja na microrregião do Alto Purus, Acre. 260 $aRio Branco, AC: Embrapa CPAF-AC$c1997 300 $a2 p. 490 $a(Embrapa CPAF-AC. Pesquisa em andamento, 113). 520 $aO presente trabalho vem sendo conduzido pela Embrapa Acre com o objetivo de avaliar o comportamento de cultivares e linhagens de soja e identificar genotipos altamente produtivos na microrregião do Alto Purus, Acre. 650 $aAcclimation 650 $aAgronomic traits 650 $aBrazil 650 $abreeding 650 $aCrop yield 650 $aEnvironmental factors 650 $aPlant breeding 650 $aprogeny 650 $asoybeans 650 $avarieties 650 $aVariety trials 650 $aAclimatação 650 $aCaracterísticas Agronômicas 650 $aComportamento de Variedade 650 $aCondição Ambiental 650 $aGlycine Max 650 $aLinhagem 650 $aMelhoramento 650 $aMelhoramento Genético Vegetal 650 $aPhaseolus Vulgaris 650 $aProdutividade 650 $aProgênie 650 $aRendimento 650 $aSeleção 650 $aSoja 650 $aVariedade 653 $aAclimatación 653 $aAcre 653 $aAlto Purus 653 $aAlto Purus (AC) 653 $aAmazonia Occidental 653 $aAmazônia Ocidental 653 $aAvaliação 653 $aBehaviour 653 $aBrasil 653 $aComportamento 653 $aCultivar 653 $aEnsayos de variedades 653 $aEvaluation 653 $aFactores ambientales 653 $aFitomejoramiento 653 $aLinhagem precoce 653 $aRendimiento de los cultivos 653 $aSemillas de soja 653 $aSoybean 653 $aWestern Amazon
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