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Registro Completo |
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
15/10/2020 |
Data da última atualização: |
12/08/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SOARES, J. C.; MELENDEZ, M. E.; SOARES, A. C.; ARANTES, L. M. R. B.; RODRIGUES, V. C.; CARVALHO, A. L.; REIS, R. M.; MATTOSO, L. H. C.; OLIVEIRA JUNIOR, O. N. |
Afiliação: |
LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA. |
Título: |
Detection of HPV16 in cell lines deriving from cervical and head and neck cancer using a genosensor made with a DNA probe on a layer-by-layer matrix. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Materials Chemistry Frontiers, v. 4, n. 11, 2020. |
Páginas: |
3258-3266 |
DOI: |
10.1039/d0qm00530d |
Idioma: |
Inglês |
Conteúdo: |
Detection of human papillomavirus 16 (HPV16) is essential for the diagnosis of cervix and head and neck cancer, which has prompted the development of biosensors to replace the sophisticated molecular techniques. In this paper, we employ a genosensor made with a HPV16 DNA probe (cpHPV16) immobilized on a layer-by-layer (LbL) film of chitosan and carbon nanotubes to detect a complementary sequence HPV16 oligo (ssHPV16). In addition to employing impedance spectroscopy, which has already been used in the literature, we detect ssHPV16 in buffer samples and in cancer cell line samples with UV-vis. spectroscopy. The limit of detection was 18.5 pmol L−1 in impedance spectroscopy measurements and 10.9 pmol L−1 in UV-vis measurements. Distinction of the various ssHPV16 concentrations and of the cancer cell line samples CasKi, SiHa, UMSCC-47, UM-SCC104 and 93-VU147T with both electrical and optical data could be made with the multidimensional projection technique referred to as interactive document mapping (IDMAP), with which we could confirm the absence of false positives by testing cell lines that did not contain ssHPV16 (JHU12, JHU13 and JHU28). The hybridization between cpHPV16 and ssHPV16 responsible for the biosensing was verified with polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS), while the concentration dependence could be modeled with a double Freundlich function. The use of optical absorbance measurements to detect ssHPV16 is promising toward a simple colorimetric detection. MenosDetection of human papillomavirus 16 (HPV16) is essential for the diagnosis of cervix and head and neck cancer, which has prompted the development of biosensors to replace the sophisticated molecular techniques. In this paper, we employ a genosensor made with a HPV16 DNA probe (cpHPV16) immobilized on a layer-by-layer (LbL) film of chitosan and carbon nanotubes to detect a complementary sequence HPV16 oligo (ssHPV16). In addition to employing impedance spectroscopy, which has already been used in the literature, we detect ssHPV16 in buffer samples and in cancer cell line samples with UV-vis. spectroscopy. The limit of detection was 18.5 pmol L−1 in impedance spectroscopy measurements and 10.9 pmol L−1 in UV-vis measurements. Distinction of the various ssHPV16 concentrations and of the cancer cell line samples CasKi, SiHa, UMSCC-47, UM-SCC104 and 93-VU147T with both electrical and optical data could be made with the multidimensional projection technique referred to as interactive document mapping (IDMAP), with which we could confirm the absence of false positives by testing cell lines that did not contain ssHPV16 (JHU12, JHU13 and JHU28). The hybridization between cpHPV16 and ssHPV16 responsible for the biosensing was verified with polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS), while the concentration dependence could be modeled with a double Freundlich function. The use of optical absorbance measurements to detect ssHPV16 is promising toward a... Mostrar Tudo |
Palavras-Chave: |
Chitosan and carbon nanotubes; Sophisticated molecular techniques. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02407naa a2200265 a 4500 001 2125561 005 2022-08-12 008 2020 bl uuuu u00u1 u #d 024 7 $a10.1039/d0qm00530d$2DOI 100 1 $aSOARES, J. C. 245 $aDetection of HPV16 in cell lines deriving from cervical and head and neck cancer using a genosensor made with a DNA probe on a layer-by-layer matrix.$h[electronic resource] 260 $c2020 300 $a3258-3266 520 $aDetection of human papillomavirus 16 (HPV16) is essential for the diagnosis of cervix and head and neck cancer, which has prompted the development of biosensors to replace the sophisticated molecular techniques. In this paper, we employ a genosensor made with a HPV16 DNA probe (cpHPV16) immobilized on a layer-by-layer (LbL) film of chitosan and carbon nanotubes to detect a complementary sequence HPV16 oligo (ssHPV16). In addition to employing impedance spectroscopy, which has already been used in the literature, we detect ssHPV16 in buffer samples and in cancer cell line samples with UV-vis. spectroscopy. The limit of detection was 18.5 pmol L−1 in impedance spectroscopy measurements and 10.9 pmol L−1 in UV-vis measurements. Distinction of the various ssHPV16 concentrations and of the cancer cell line samples CasKi, SiHa, UMSCC-47, UM-SCC104 and 93-VU147T with both electrical and optical data could be made with the multidimensional projection technique referred to as interactive document mapping (IDMAP), with which we could confirm the absence of false positives by testing cell lines that did not contain ssHPV16 (JHU12, JHU13 and JHU28). The hybridization between cpHPV16 and ssHPV16 responsible for the biosensing was verified with polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS), while the concentration dependence could be modeled with a double Freundlich function. The use of optical absorbance measurements to detect ssHPV16 is promising toward a simple colorimetric detection. 653 $aChitosan and carbon nanotubes 653 $aSophisticated molecular techniques 700 1 $aMELENDEZ, M. E. 700 1 $aSOARES, A. C. 700 1 $aARANTES, L. M. R. B. 700 1 $aRODRIGUES, V. C. 700 1 $aCARVALHO, A. L. 700 1 $aREIS, R. M. 700 1 $aMATTOSO, L. H. C. 700 1 $aOLIVEIRA JUNIOR, O. N. 773 $tMaterials Chemistry Frontiers$gv. 4, n. 11, 2020.
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Embrapa Instrumentação (CNPDIA) |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
28/08/2001 |
Data da última atualização: |
20/09/2004 |
Autoria: |
VIDAL, R. A.; FLECK, N. G. |
Título: |
Controle de Girassol Espontaneo com Herbicidas Pos-Emergentes Seletivos para Soja. |
Ano de publicação: |
1992 |
Fonte/Imprenta: |
Pesquisa Agropecuaria Brasileira, Brasilia, v.27, n.4, p.549-560, abr.1992 |
Idioma: |
Português |
Conteúdo: |
Conduziu-se um experimento na Estacao Experimental Agronomica da Universidade Federal do Rio Grande do Sul, em Eldorado do Sul, RS, em 1989/90, para procurar alternativas para solucionar o problema da presenca de plantas espontaneas de girassol na cultura de soja. Foram avaliados os herbicidas acifluorfen, acifluorfen + bentazon, bentazon, clomazona, clorimuronetil, imazaquin, imazetapir, fomesafen e lactofen, utilizados na dose recomendada e aspergidos em girassol nos estadios de desenvolvimento de quatro e dez folhas. O controle de girassol com clomazona, clorimuron, bentazon e bentazon + acifluorfen foi dependente do estadio de desenvolvimento das plantas, obtendo-se controle adequado quando os produtos foram aspergidos em plantas com quatro folhas. O controle de girassol com imazaquin e imazetapir foi independente do estadio de desenvolvimento, obtendo-se controle satisfatorio quando os produtos foram aplicados em ambos os estadios. Acifluorfen, fomesafen e lactofen, aspergidos em quaisquer das duas epocas, nao foram eficientes no controle de girassol. |
Palavras-Chave: |
clomazona; clorimuronetil; imazetapir. |
Thesagro: |
Glycine Max; Helianthus Annuus. |
Thesaurus NAL: |
acifluorfen; bentazon; fomesafen; imazaquin; lactofen. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/20670/1/pab03_abr_92.pdf
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Marc: |
LEADER 01756naa a2200253 a 4500 001 1105605 005 2004-09-20 008 1992 bl uuuu u00u1 u #d 100 1 $aVIDAL, R. A. 245 $aControle de Girassol Espontaneo com Herbicidas Pos-Emergentes Seletivos para Soja. 260 $c1992 520 $aConduziu-se um experimento na Estacao Experimental Agronomica da Universidade Federal do Rio Grande do Sul, em Eldorado do Sul, RS, em 1989/90, para procurar alternativas para solucionar o problema da presenca de plantas espontaneas de girassol na cultura de soja. Foram avaliados os herbicidas acifluorfen, acifluorfen + bentazon, bentazon, clomazona, clorimuronetil, imazaquin, imazetapir, fomesafen e lactofen, utilizados na dose recomendada e aspergidos em girassol nos estadios de desenvolvimento de quatro e dez folhas. O controle de girassol com clomazona, clorimuron, bentazon e bentazon + acifluorfen foi dependente do estadio de desenvolvimento das plantas, obtendo-se controle adequado quando os produtos foram aspergidos em plantas com quatro folhas. O controle de girassol com imazaquin e imazetapir foi independente do estadio de desenvolvimento, obtendo-se controle satisfatorio quando os produtos foram aplicados em ambos os estadios. Acifluorfen, fomesafen e lactofen, aspergidos em quaisquer das duas epocas, nao foram eficientes no controle de girassol. 650 $aacifluorfen 650 $abentazon 650 $afomesafen 650 $aimazaquin 650 $alactofen 650 $aGlycine Max 650 $aHelianthus Annuus 653 $aclomazona 653 $aclorimuronetil 653 $aimazetapir 700 1 $aFLECK, N. G. 773 $tPesquisa Agropecuaria Brasileira, Brasilia$gv.27, n.4, p.549-560, abr.1992
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