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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
02/01/2008 |
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Data da última atualização: |
08/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERREIRA, C. R.; MEIRELLES, F. V.; YAMAZAKI, W.; CHIARATTI, M. R.; NICIURA, S. C. M.; PERECIN, F.; SMITH, L. C.; GARCIA, J. M. |
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Afiliação: |
CHRISTINA RAMIRES FERREIRA, UNESP; FLÁVIO VIEIRA MEIRELLES, USP; WALT YAMAZAKI, UNESP; MARCOS ROBERTO CHIARATTI, USP; SIMONE CRISTINA MEO NICIURA, CPPSE; FELIPE PERECIN, UNESP; LAWRENCE CHARLES SMITH, UNIVERSITÉ DE MONTRÉAL; JOAQUIM MANSANO GARCIA, UNESP. |
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Título: |
The kinetics of donor cell mtDNA in embryonic and somatic donor cell-derived bovine embryos. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Cloning and Stem Cells. v. 9, n. 4, p. 618-629, dec. 2007. |
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DOI: |
10.1089/clo.2006.0082 |
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Idioma: |
Inglês |
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Conteúdo: |
The mechanisms controlling the outcome of donor cell-derived mitochondrial DNA (mtDNA) in cloned animals remain largely unknown. This research was designed to investigate the kinetics of somatic and embryonic mtDNA in reconstructed bovine embryos during preimplantation development, as well as in cloned animals. The experiment involved two different procedures of embryo reconstruction and their evaluation at five distinct phases of embryo development to measure the proportion of donor cell mtDNA (Bos indicus), as well as the segregation of this mtDNA during cleavage. The ratio of donor cell (B. indicus) to host oocyte (B. taurus) mtDNA (heteroplasmy) from blastomere(NT-B) and fibroblast(NT-F) reconstructed embryos was estimated using an allele-specific PCR with fluorochrome-stained specific primers in each sampled blastomere, in whole blastocysts, and in the tissues of a fibroblast derived newborn clone. NT-B zygotes and blastocysts show similar levels of heteroplasmy (11.0% and 14.0%, respectively), despite a significant decrease at the 916 cell stage (5.8%; p < 0.05). Heteroplasmy levels in NT-F reconstructed zygotes, however, increased from an initial low level (4.7%), to 12.9% (p < 0.05) at the 9?16 cell stage. The NT-F blastocysts contained low levels of heteroplasmy (2.2%) and no somatic-derived mtDNA was detected in the gametes or the tissues of the newborn calf cloned. These results suggest that, in contrast to the mtDNA of blastomeres, that of somatic cells either undergoes replication or escapes degradation during cleavage, although it is degraded later after the blastocyst stage or lost during somatic development, as revealed by the lack of donor cell mtDNA at birth. MenosThe mechanisms controlling the outcome of donor cell-derived mitochondrial DNA (mtDNA) in cloned animals remain largely unknown. This research was designed to investigate the kinetics of somatic and embryonic mtDNA in reconstructed bovine embryos during preimplantation development, as well as in cloned animals. The experiment involved two different procedures of embryo reconstruction and their evaluation at five distinct phases of embryo development to measure the proportion of donor cell mtDNA (Bos indicus), as well as the segregation of this mtDNA during cleavage. The ratio of donor cell (B. indicus) to host oocyte (B. taurus) mtDNA (heteroplasmy) from blastomere(NT-B) and fibroblast(NT-F) reconstructed embryos was estimated using an allele-specific PCR with fluorochrome-stained specific primers in each sampled blastomere, in whole blastocysts, and in the tissues of a fibroblast derived newborn clone. NT-B zygotes and blastocysts show similar levels of heteroplasmy (11.0% and 14.0%, respectively), despite a significant decrease at the 916 cell stage (5.8%; p < 0.05). Heteroplasmy levels in NT-F reconstructed zygotes, however, increased from an initial low level (4.7%), to 12.9% (p < 0.05) at the 9?16 cell stage. The NT-F blastocysts contained low levels of heteroplasmy (2.2%) and no somatic-derived mtDNA was detected in the gametes or the tissues of the newborn calf cloned. These results suggest that, in contrast to the mtDNA of blastomeres, that of somatic cells either un... Mostrar Tudo |
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Palavras-Chave: |
Bovine embryos; Cell mtDNA; Embryonic. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02452naa a2200253 a 4500
001 1048156
005 2023-03-08
008 2007 bl uuuu u00u1 u #d
024 7 $a10.1089/clo.2006.0082$2DOI
100 1 $aFERREIRA, C. R.
245 $aThe kinetics of donor cell mtDNA in embryonic and somatic donor cell-derived bovine embryos.$h[electronic resource]
260 $c2007
520 $aThe mechanisms controlling the outcome of donor cell-derived mitochondrial DNA (mtDNA) in cloned animals remain largely unknown. This research was designed to investigate the kinetics of somatic and embryonic mtDNA in reconstructed bovine embryos during preimplantation development, as well as in cloned animals. The experiment involved two different procedures of embryo reconstruction and their evaluation at five distinct phases of embryo development to measure the proportion of donor cell mtDNA (Bos indicus), as well as the segregation of this mtDNA during cleavage. The ratio of donor cell (B. indicus) to host oocyte (B. taurus) mtDNA (heteroplasmy) from blastomere(NT-B) and fibroblast(NT-F) reconstructed embryos was estimated using an allele-specific PCR with fluorochrome-stained specific primers in each sampled blastomere, in whole blastocysts, and in the tissues of a fibroblast derived newborn clone. NT-B zygotes and blastocysts show similar levels of heteroplasmy (11.0% and 14.0%, respectively), despite a significant decrease at the 916 cell stage (5.8%; p < 0.05). Heteroplasmy levels in NT-F reconstructed zygotes, however, increased from an initial low level (4.7%), to 12.9% (p < 0.05) at the 9?16 cell stage. The NT-F blastocysts contained low levels of heteroplasmy (2.2%) and no somatic-derived mtDNA was detected in the gametes or the tissues of the newborn calf cloned. These results suggest that, in contrast to the mtDNA of blastomeres, that of somatic cells either undergoes replication or escapes degradation during cleavage, although it is degraded later after the blastocyst stage or lost during somatic development, as revealed by the lack of donor cell mtDNA at birth.
653 $aBovine embryos
653 $aCell mtDNA
653 $aEmbryonic
700 1 $aMEIRELLES, F. V.
700 1 $aYAMAZAKI, W.
700 1 $aCHIARATTI, M. R.
700 1 $aNICIURA, S. C. M.
700 1 $aPERECIN, F.
700 1 $aSMITH, L. C.
700 1 $aGARCIA, J. M.
773 $tCloning and Stem Cells.$gv. 9, n. 4, p. 618-629, dec. 2007.
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Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
22/02/2013 |
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Data da última atualização: |
11/07/2018 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
COSTA, M. R.; OLIVEIRA, M. do S. P. de; NASCIMENTO, S. V. do; SILVA, C. dos A. da. |
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Afiliação: |
MARIA ROSA TRAVASSOS DA ROSA COSTA, CPATU; MARIA DO SOCORRO P DE OLIVEIRA, CPATU; SIDNEY VASCONCELOS DO NASCIMENTO, ESTAGIÁRIO CPATU; CLARISSA DOS ANJOS DA SILVA, ESTAGIÁRIA CPATU. |
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Título: |
Transferibilidade de inciadores microssatélites de açaizeiro (Euterpe oleracea) para dendenzeiro (Elaeis guinensis). |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
Considerando o tempo necessário para o desenvolvimento de iniciadores específicos, a análise da transferibilidade entre espécies é bastante oportuna. O objetivo deste trabalho foi avaliar a transferibilidade de seis iniciadores microssatélites desenvolvidos para o açaizeiro em dendezeiro. O material analisado foi composto de onze genótipos de dendezeiro e as amplificações foram feitas pela técnica da PCR. Os resultados mostraram a viabilidade da utilização de cinco inciadores EE2, EE3, EE8, EE15, EE43 em estudos de melhoramento e variabilidade genética em dendezeiro. |
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Palavras-Chave: |
Marcadores moleculares; Recursos genéticos; Variabilidade. |
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Thesagro: |
Açaí; Dendê. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/77055/1/431.pdf
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Marc: |
LEADER 01342nam a2200217 a 4500
001 1950596
005 2018-07-11
008 2012 bl uuuu u00u1 u #d
100 1 $aCOSTA, M. R.
245 $aTransferibilidade de inciadores microssatélites de açaizeiro (Euterpe oleracea) para dendenzeiro (Elaeis guinensis).
260 $aIn: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos$c2012
300 $c1 CD-ROM.
520 $aConsiderando o tempo necessário para o desenvolvimento de iniciadores específicos, a análise da transferibilidade entre espécies é bastante oportuna. O objetivo deste trabalho foi avaliar a transferibilidade de seis iniciadores microssatélites desenvolvidos para o açaizeiro em dendezeiro. O material analisado foi composto de onze genótipos de dendezeiro e as amplificações foram feitas pela técnica da PCR. Os resultados mostraram a viabilidade da utilização de cinco inciadores EE2, EE3, EE8, EE15, EE43 em estudos de melhoramento e variabilidade genética em dendezeiro.
650 $aAçaí
650 $aDendê
653 $aMarcadores moleculares
653 $aRecursos genéticos
653 $aVariabilidade
700 1 $aOLIVEIRA, M. do S. P. de
700 1 $aNASCIMENTO, S. V. do
700 1 $aSILVA, C. dos A. da
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