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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
22/04/2016 |
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Data da última atualização: |
10/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CERRO, P. del; ROLLA-SANTOS, A. A. P.; VALDERRAMA-FERNANDEZ, R.; GIL-SERRANO, A.; BELLOGÍN, R. A.; GOMES, D. F.; MONTAÑO, F. P.; MEGÍAS, M.; HUNGRIA, M.; JAVIER OLLERO, F. |
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Afiliação: |
PABLO DEL CERRO, Universidad de Sevilla; AMANDA A. P. ROLLA SANTOS, Pós-graduanda; ROCÍO VALDERRAMA FERNANDEZ, Universidad de Sevilla; ANTONIO GIL SERRANO, Universidad de Sevilla; RAMÓN A. BELLOGÍN, Universidad de Sevilla; DOUGLAS FABIANO GOMES, Pós-graduando; FRANCISCO PÉREZ MONTAÑO, Universidad de Sevilla; MANUEL MEGÍAS, Universidad de Sevilla; MARIANGELA HUNGRIA DA CUNHA, CNPSO; FRANCISCO JAVIER OLLERO, Universidad de Sevilla. |
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Título: |
NrcR, a new transcriptional regulator of Rhizobium tropici CIAT 899 involved in the Legume root-nodule symbiosis. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
PLOS One, v. 11, n. 4, e0154029, Apr. 2016. |
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ISSN: |
1932-6203 |
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DOI: |
10.1371/journal.pone.0154029 |
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Idioma: |
Inglês |
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Conteúdo: |
The establishment of nitrogen-fixing rhizobium-legume symbioses requires a highly complex cascade of events. In this molecular dialogue the bacterial NodD transcriptional regulators in conjunction with plant inducers, mostly flavonoids, are responsible for the biosynthesis and secretion of Nod factors which are key molecules for successful nodulation. Other transcriptional regulators related to the symbiotic process have been identified in rhizobial genomes, including negative regulators such as NolR. Rhizobium tropici CIAT 899 is an important symbiont of common bean (Phaseolus vulgaris L.), and its genome encompasses intriguing features such as five copies of nodD genes, as well as other possible transcriptional regulators including the NolR protein. Here we describe and characterize a new regulatory gene located in the non-symbiotic plasmid pRtrCIAT899c, that shows homology (46% identity) with the nolR gene located in the chromosome of CIAT 899. The mutation of this gene, named nrcR (nolR-like plasmid c Regulator), enhanced motility and exopolysaccharide production in comparison to the wild-type strain. Interestingly, the number and decoration of Nod Factors produced by this mutant were higher than those detected in the wildtype strain, especially under salinity stress. The nrcR mutant showed delayed nodulation and reduced competitiveness with P. vulgaris, and reduction in nodule number and shoot dry weight in both P. vulgaris and Leucaena leucocephala. Moreover, the mutant exhibited reduced capacity to induce the nodC gene in comparison to the wild-type CIAT 899. The finding of a new nod-gene regulator located in a non-symbiotic plasmid may reveal the existence of even more complex mechanisms of regulation of nodulation genes in R. tropici CIAT 899 that may be applicable to other rhizobial species. MenosThe establishment of nitrogen-fixing rhizobium-legume symbioses requires a highly complex cascade of events. In this molecular dialogue the bacterial NodD transcriptional regulators in conjunction with plant inducers, mostly flavonoids, are responsible for the biosynthesis and secretion of Nod factors which are key molecules for successful nodulation. Other transcriptional regulators related to the symbiotic process have been identified in rhizobial genomes, including negative regulators such as NolR. Rhizobium tropici CIAT 899 is an important symbiont of common bean (Phaseolus vulgaris L.), and its genome encompasses intriguing features such as five copies of nodD genes, as well as other possible transcriptional regulators including the NolR protein. Here we describe and characterize a new regulatory gene located in the non-symbiotic plasmid pRtrCIAT899c, that shows homology (46% identity) with the nolR gene located in the chromosome of CIAT 899. The mutation of this gene, named nrcR (nolR-like plasmid c Regulator), enhanced motility and exopolysaccharide production in comparison to the wild-type strain. Interestingly, the number and decoration of Nod Factors produced by this mutant were higher than those detected in the wildtype strain, especially under salinity stress. The nrcR mutant showed delayed nodulation and reduced competitiveness with P. vulgaris, and reduction in nodule number and shoot dry weight in both P. vulgaris and Leucaena leucocephala. Moreover, the mutan... Mostrar Tudo |
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Thesagro: |
Fixação de nitrogenio. |
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Thesaurus Nal: |
Nitrogen fixation. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142515/1/NrcR-a-new-transcriptional....pdf
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Marc: |
LEADER 02681naa a2200277 a 4500
001 2043848
005 2017-07-10
008 2016 bl uuuu u00u1 u #d
022 $a1932-6203
024 7 $a10.1371/journal.pone.0154029$2DOI
100 1 $aCERRO, P. del
245 $aNrcR, a new transcriptional regulator of Rhizobium tropici CIAT 899 involved in the Legume root-nodule symbiosis.$h[electronic resource]
260 $c2016
520 $aThe establishment of nitrogen-fixing rhizobium-legume symbioses requires a highly complex cascade of events. In this molecular dialogue the bacterial NodD transcriptional regulators in conjunction with plant inducers, mostly flavonoids, are responsible for the biosynthesis and secretion of Nod factors which are key molecules for successful nodulation. Other transcriptional regulators related to the symbiotic process have been identified in rhizobial genomes, including negative regulators such as NolR. Rhizobium tropici CIAT 899 is an important symbiont of common bean (Phaseolus vulgaris L.), and its genome encompasses intriguing features such as five copies of nodD genes, as well as other possible transcriptional regulators including the NolR protein. Here we describe and characterize a new regulatory gene located in the non-symbiotic plasmid pRtrCIAT899c, that shows homology (46% identity) with the nolR gene located in the chromosome of CIAT 899. The mutation of this gene, named nrcR (nolR-like plasmid c Regulator), enhanced motility and exopolysaccharide production in comparison to the wild-type strain. Interestingly, the number and decoration of Nod Factors produced by this mutant were higher than those detected in the wildtype strain, especially under salinity stress. The nrcR mutant showed delayed nodulation and reduced competitiveness with P. vulgaris, and reduction in nodule number and shoot dry weight in both P. vulgaris and Leucaena leucocephala. Moreover, the mutant exhibited reduced capacity to induce the nodC gene in comparison to the wild-type CIAT 899. The finding of a new nod-gene regulator located in a non-symbiotic plasmid may reveal the existence of even more complex mechanisms of regulation of nodulation genes in R. tropici CIAT 899 that may be applicable to other rhizobial species.
650 $aNitrogen fixation
650 $aFixação de nitrogenio
700 1 $aROLLA-SANTOS, A. A. P.
700 1 $aVALDERRAMA-FERNANDEZ, R.
700 1 $aGIL-SERRANO, A.
700 1 $aBELLOGÍN, R. A.
700 1 $aGOMES, D. F.
700 1 $aMONTAÑO, F. P.
700 1 $aMEGÍAS, M.
700 1 $aHUNGRIA, M.
700 1 $aJAVIER OLLERO, F.
773 $tPLOS One$gv. 11, n. 4, e0154029, Apr. 2016.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria Tropical; Embrapa Algodão. |
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Data corrente: |
07/01/2020 |
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Data da última atualização: |
20/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ANDRADE, F. K.; MORAIS, J. P. S.; MUNIZ, C. R.; NASCIMENTO, J. H. O. DO; VIEIRA, R. S.; GAMA, F. M. P. DA; ROSA, M. de F. |
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Afiliação: |
FÁBIA KARINE ANDRADE, Universidade Federal do Ceará - UFC/Departamento de Engenharia Química; JOAO PAULO SARAIVA MORAIS, CNPA; CELLI RODRIGUES MUNIZ, CNPAT; JOSÉ HERIBERTO OLIVEIRA DO NASCIMENTO, Universidade Federal doRio Grande do Norte - UFRN/Departamento de Engenharia; RODRIGO SILVEIRA VIEIRA, Universidade Federal do Ceará - UFC/Departamento de Engenharia Química; FRANCISCO MIGUEL PORTELA DA GAMA, IBB, Institute for Biotechnology and Bioengineering,University of Minho, Braga, Portugal; MORSYLEIDE DE FREITAS ROSA, CNPAT. |
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Título: |
Stable microfluidized bacterial cellulose suspension. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Cellulose, v. 26, n. 10, p. 5851-5864, July 2019. |
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DOI: |
. DOI: https://doi.org/10.1007/s10570-019-02512-y |
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Idioma: |
Inglês |
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Conteúdo: |
In this work, nanofibrillated suspensions of bacterial cellulose (BC) were produced via microfluidization. The effects of the size of the openings of the microfluidizer chamber and ultrasonication on the nanofibril properties were evaluated. The results of the X-ray diffraction analysis indicated a considerable reduction in BC crystallinity (86?65%) and crystallite size (5.8?4.0 nm) after microfluidization and ultrasonication. Thermal analysis showed a remarkable reduction from 337 to 283ªC in the initial temperature of degradation along the several steps of BC deconstruction. Moreover, infrared analysis indicated that both processes led to an increase in the Ib content (43?66%) of the fibers. Morphological analysis showed that the fibrillation process used exposed the internal faces of the ribbon-like nanofibrils, and thus, increased the surface area of the cellulose network, and produced fibers with a high aspect ratio (L/d). A thermally stable nanofibrillated suspension could be obtained by adding carboxymethyl cellulose as a simple and effective way to maintain cellulose fibers dispersed in the solution during sterilization by autoclaving. |
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Palavras-Chave: |
Celulose bacteriana; Celulose nanofibrilada; Microfluidização; Microfluidization; Nanofibrillated cellulose; Sterilization. |
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Thesagro: |
Celulose; Esterilização. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208760/1/Stable-microfluidized-bacterial.pdf
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Marc: |
LEADER 02053naa a2200301 a 4500
001 2118741
005 2020-01-20
008 2019 bl uuuu u00u1 u #d
024 7 $a. DOI: https://doi.org/10.1007/s10570-019-02512-y$2DOI
100 1 $aANDRADE, F. K.
245 $aStable microfluidized bacterial cellulose suspension.$h[electronic resource]
260 $c2019
520 $aIn this work, nanofibrillated suspensions of bacterial cellulose (BC) were produced via microfluidization. The effects of the size of the openings of the microfluidizer chamber and ultrasonication on the nanofibril properties were evaluated. The results of the X-ray diffraction analysis indicated a considerable reduction in BC crystallinity (86?65%) and crystallite size (5.8?4.0 nm) after microfluidization and ultrasonication. Thermal analysis showed a remarkable reduction from 337 to 283ªC in the initial temperature of degradation along the several steps of BC deconstruction. Moreover, infrared analysis indicated that both processes led to an increase in the Ib content (43?66%) of the fibers. Morphological analysis showed that the fibrillation process used exposed the internal faces of the ribbon-like nanofibrils, and thus, increased the surface area of the cellulose network, and produced fibers with a high aspect ratio (L/d). A thermally stable nanofibrillated suspension could be obtained by adding carboxymethyl cellulose as a simple and effective way to maintain cellulose fibers dispersed in the solution during sterilization by autoclaving.
650 $aCelulose
650 $aEsterilização
653 $aCelulose bacteriana
653 $aCelulose nanofibrilada
653 $aMicrofluidização
653 $aMicrofluidization
653 $aNanofibrillated cellulose
653 $aSterilization
700 1 $aMORAIS, J. P. S.
700 1 $aMUNIZ, C. R.
700 1 $aNASCIMENTO, J. H. O. DO
700 1 $aVIEIRA, R. S.
700 1 $aGAMA, F. M. P. DA
700 1 $aROSA, M. de F.
773 $tCellulose$gv. 26, n. 10, p. 5851-5864, July 2019.
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Embrapa Algodão (CNPA) |
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