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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
26/02/2016 |
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Data da última atualização: |
26/02/2016 |
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Autoria: |
ECHELARD, Y.; ZIOMEK, C. A.; MEADE, H. M. |
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Título: |
Expression of recombinant proteins in the milk of transgenic goats. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON GOATS, 7., 2000, Tours, Poitiers, France. Proceedings... Paris: INRA: IGA: Institut de Elevage, 2000. v. 1, p. 25-29. |
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ISBN: |
2-84148-038-0 |
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Idioma: |
Inglês |
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Conteúdo: |
Recombinant proteins are incresingly being used as therapeutic agents. Clinical applications often require large amounts of highly purified molecules, often for chronic treatments. Produciton in the milk of transgenic dairy animals is currently being tested as an alternative to plasma fractionation for the manufacture of a number of blood factors, recombinant antibodies and growth factors. The following review examines the potential of transgenic goats for the production of recombinant therapeutic proteins. To date, more than 60 proteins have been expressed in the milk of transgenic mice, rabbits, goats, and dairy cows. A factor in the generation of transgenic goats for the production of therapeutic protein in milkis the method employed to introduce the exogenous DNA in the germline. To date, the method most frequently employed to produce trangenic goats has been pronuclear microinjection. While successful and widely used, the microinjection approach has had limited efficiency.. For most proteins, expression in th range of 3 - 5 grams/ liter do not seeem to adversely affect lactation volumes. It is possible to achieve high-level expression of recombinant therapeutic proteins in the milk of transgenic animals. This technology could also be useful for the improvement of carine dairy herds, especially increasing the yield of cheese. |
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Palavras-Chave: |
Injecção; Mutation breeding; Nucleus. |
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Thesagro: |
Animal transgênico; Anticorpo; Cabra; Caprino; DNA; Engenharia genética; Leite; Medicamento; Melhoramento animal; Mutação; Núcleo; Proteína. |
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Thesaurus Nal: |
Animal breeding; Antibody formation; Drug therapy; Drugs; Gene expression; Genetic engineering; Goats; Injection; Milk; Proteins; Transgenic animals. |
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Categoria do assunto: |
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Marc: |
LEADER 02607naa a2200469 a 4500
001 2038725
005 2016-02-26
008 2000 bl uuuu u00u1 u #d
020 $a2-84148-038-0
100 1 $aECHELARD, Y.
245 $aExpression of recombinant proteins in the milk of transgenic goats.
260 $c2000
520 $aRecombinant proteins are incresingly being used as therapeutic agents. Clinical applications often require large amounts of highly purified molecules, often for chronic treatments. Produciton in the milk of transgenic dairy animals is currently being tested as an alternative to plasma fractionation for the manufacture of a number of blood factors, recombinant antibodies and growth factors. The following review examines the potential of transgenic goats for the production of recombinant therapeutic proteins. To date, more than 60 proteins have been expressed in the milk of transgenic mice, rabbits, goats, and dairy cows. A factor in the generation of transgenic goats for the production of therapeutic protein in milkis the method employed to introduce the exogenous DNA in the germline. To date, the method most frequently employed to produce trangenic goats has been pronuclear microinjection. While successful and widely used, the microinjection approach has had limited efficiency.. For most proteins, expression in th range of 3 - 5 grams/ liter do not seeem to adversely affect lactation volumes. It is possible to achieve high-level expression of recombinant therapeutic proteins in the milk of transgenic animals. This technology could also be useful for the improvement of carine dairy herds, especially increasing the yield of cheese.
650 $aAnimal breeding
650 $aAntibody formation
650 $aDrug therapy
650 $aDrugs
650 $aGene expression
650 $aGenetic engineering
650 $aGoats
650 $aInjection
650 $aMilk
650 $aProteins
650 $aTransgenic animals
650 $aAnimal transgênico
650 $aAnticorpo
650 $aCabra
650 $aCaprino
650 $aDNA
650 $aEngenharia genética
650 $aLeite
650 $aMedicamento
650 $aMelhoramento animal
650 $aMutação
650 $aNúcleo
650 $aProteína
653 $aInjecção
653 $aMutation breeding
653 $aNucleus
700 1 $aZIOMEK, C. A.
700 1 $aMEADE, H. M.
773 $tIn: INTERNATIONAL CONFERENCE ON GOATS, 7., 2000, Tours, Poitiers, France. Proceedings... Paris: INRA: IGA: Institut de Elevage, 2000.$gv. 1, p. 25-29.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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