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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
13/01/2009 |
Data da última atualização: |
14/01/2009 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
MACHADO, M. A.; ÁSTUA, J. F.; AMARAL, A. M. do; MALOSSO, A.; MAZZAFERA, P. |
Afiliação: |
M. A. Machado, APTA; Juliana Freitas-Ástua, CNPMF/APTA; A. M. do Amaral, APTA; A. Malosso, APTA; P. Mazzafera, APTA. |
Título: |
Gene expression of carotenoid biosynthesis pathway and carotenoid content in different varieties of sweet orange (Citrus sinensis L. Osb.). |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan,China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 60. 105. |
Idioma: |
Inglês |
Conteúdo: |
Varieties of sweet orange are usually distinguishable by the content and quality of the juice. Such traits are important to characterize early, middle and late varieties. Juice quality is a complex trait that changes according with the content and quality of carotenoids, flavanoids, sugars and organic acids. The balance among such compounds is the main characteristic typifies the fruits of almost all varieties of sweet orange. The studies of biochemical pathway of carotenoid synthesis are hindered by the difficulties to evaluate enzyme activities which substracts and products are hydrophobic compounds. Thus the goal of this work was to evaluate the expression of genes of carotenoid synthesis in fruits of sweet orange Hamlin (early), Pera (middle), and Valencia (late), and to correlate with the content of carotenoids during fruit development. A pool of three fruits of 12 years old trees of sweet orange grafted on Rangpur lime was evaluate for fruit quality (juice content, Brix, ratio, content of total carotenoids), gene expression by Rt-qPCR in flavedo (epicarp) (Phytoene synthase, psy; Phytoene desaturase, pds; Z-carotene desaturase, zds; Carotenoid isomerase, crtiso; Lycopene cyclase, lcyb; Beta-hydroxylase, hyb; Zexanthin epoxidase, zep; Lycopene cyclase, lcye). Primers design was based on CitEST and public database. Rt-qPCR was performed using Sybergreen. The higher gene expression (7 to 19 times) was in sweet orange Hamlin at the end of the season (June, in Brazil), pointed out an active synthesis varieties the higher expression was in the middle of the cycle (July) before the picking time. Not always a direct relationship between gene expression and content of carotenoids could be inferred. MenosVarieties of sweet orange are usually distinguishable by the content and quality of the juice. Such traits are important to characterize early, middle and late varieties. Juice quality is a complex trait that changes according with the content and quality of carotenoids, flavanoids, sugars and organic acids. The balance among such compounds is the main characteristic typifies the fruits of almost all varieties of sweet orange. The studies of biochemical pathway of carotenoid synthesis are hindered by the difficulties to evaluate enzyme activities which substracts and products are hydrophobic compounds. Thus the goal of this work was to evaluate the expression of genes of carotenoid synthesis in fruits of sweet orange Hamlin (early), Pera (middle), and Valencia (late), and to correlate with the content of carotenoids during fruit development. A pool of three fruits of 12 years old trees of sweet orange grafted on Rangpur lime was evaluate for fruit quality (juice content, Brix, ratio, content of total carotenoids), gene expression by Rt-qPCR in flavedo (epicarp) (Phytoene synthase, psy; Phytoene desaturase, pds; Z-carotene desaturase, zds; Carotenoid isomerase, crtiso; Lycopene cyclase, lcyb; Beta-hydroxylase, hyb; Zexanthin epoxidase, zep; Lycopene cyclase, lcye). Primers design was based on CitEST and public database. Rt-qPCR was performed using Sybergreen. The higher gene expression (7 to 19 times) was in sweet orange Hamlin at the end of the season (June, in Brazil), poi... Mostrar Tudo |
Palavras-Chave: |
Citros. |
Thesagro: |
Variedade; Vitamina. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02435naa a2200205 a 4500 001 1655661 005 2009-01-14 008 2008 bl uuuu u00u1 u #d 100 1 $aMACHADO, M. A. 245 $aGene expression of carotenoid biosynthesis pathway and carotenoid content in different varieties of sweet orange (Citrus sinensis L. Osb.). 260 $c2008 520 $aVarieties of sweet orange are usually distinguishable by the content and quality of the juice. Such traits are important to characterize early, middle and late varieties. Juice quality is a complex trait that changes according with the content and quality of carotenoids, flavanoids, sugars and organic acids. The balance among such compounds is the main characteristic typifies the fruits of almost all varieties of sweet orange. The studies of biochemical pathway of carotenoid synthesis are hindered by the difficulties to evaluate enzyme activities which substracts and products are hydrophobic compounds. Thus the goal of this work was to evaluate the expression of genes of carotenoid synthesis in fruits of sweet orange Hamlin (early), Pera (middle), and Valencia (late), and to correlate with the content of carotenoids during fruit development. A pool of three fruits of 12 years old trees of sweet orange grafted on Rangpur lime was evaluate for fruit quality (juice content, Brix, ratio, content of total carotenoids), gene expression by Rt-qPCR in flavedo (epicarp) (Phytoene synthase, psy; Phytoene desaturase, pds; Z-carotene desaturase, zds; Carotenoid isomerase, crtiso; Lycopene cyclase, lcyb; Beta-hydroxylase, hyb; Zexanthin epoxidase, zep; Lycopene cyclase, lcye). Primers design was based on CitEST and public database. Rt-qPCR was performed using Sybergreen. The higher gene expression (7 to 19 times) was in sweet orange Hamlin at the end of the season (June, in Brazil), pointed out an active synthesis varieties the higher expression was in the middle of the cycle (July) before the picking time. Not always a direct relationship between gene expression and content of carotenoids could be inferred. 650 $aVariedade 650 $aVitamina 653 $aCitros 700 1 $aÁSTUA, J. F. 700 1 $aAMARAL, A. M. do 700 1 $aMALOSSO, A. 700 1 $aMAZZAFERA, P. 773 $tIn: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan,China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 60. 105.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registros recuperados : 29 | |
2. | | FERREIRA, L. P.; GEROMEL, C.; CAVALAR, A. A.; MARRACCINI, P.; MAZZAFERA, P.; PEREIRA, L. F. P.; MAZZAFERA, P. Caracterização molecular de enzimas chave do metabolismo de açúcares em café. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 3., 2003, Porto Seguro. Resumos... Brasília, DF: Embrapa Café, 2003.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Café. |
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6. | | CONCEIÇÃO, H. E. O. da; MAZZAFERA, P.; ROCHA NETO, O. G. da; STEIN, R. L. B. Composição bioquímica e enzimas oxidativas em folhas de cupuaçuzeiro (Theobroma grandiflorum (Wildenow ex Sprengel) Schumann) infectadas por Crinipellis perniciosa. In: SEMINÁRIO INTERNACIONAL SOBRE PIMENTA-DO-REINO E CUPUAÇU, 1., 1996, Belém, PA. Resumos. Belém, PA: EMBRAPA-CPATU: JICA, 1996. p. 35. (EMBRAPA-CPATU. Documentos, 88).Biblioteca(s): Embrapa Amazônia Oriental. |
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7. | | CONCEIÇÃO, H. E. O. da; MAZZAFERA, P.; ROCHA NETO, O. G. da; STEINL, R. L. B. Composição bioquímica e enzimas oxidativas em folhas de cupuaçuzeiro (Theobroma grandiflorum (Willdenox ex Sprengel) Shumann) infectadas por Crinipellis perniciosa. In: SEMINÁRIO INTERNACIONAL SOBRE PIMENTA-DO-REINO E CUPUAÇU, 1996, Belém, PA. Anais... Belém, PA: Embrapa-CPATU: JICA, 1997. p. 173-181. (Embrapa-CPATU. Documentos, 89).Biblioteca(s): Embrapa Amazônia Oriental. |
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8. | | MACHADO, M. A.; ÁSTUA, J. F.; AMARAL, A. M. do; MALOSSO, A.; MAZZAFERA, P. Gene expression of carotenoid biosynthesis pathway and carotenoid content in different varieties of sweet orange (Citrus sinensis L. Osb.). In: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan,China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 60. 105.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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11. | | NOBILE, P. M.; QUECINI, V.; BAZZO, B.; QUITERIO, G.; MAZZAFERA, P.; COLOMBO, C. A. Transcriptional profile of genes involved in the byosynthesis of phytate and ferritin in coffea. Journal of Agricultural and Food Chemistry, Washington DC, v. 58, n. 6, p. 3479-3487, mar. 2010.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Uva e Vinho. |
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12. | | GEROMEL, C.; MAZZAFERA, P.; MARRACCINI, P. R.; FERREIRA, L. P.; VIEIRA, L. G. E.; PEREIRA, L. F. P. Açúcares solúveis, sacarose sintase e sacarose fosfato sintase durante o desenvolvimento do fruto de café, sob diferentes condições In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia. Anais... Brasília, DF: Embrapa Café, 2007.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Café. |
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13. | | GEROMEL, C.; MAZZAFERA, P.; MARRACCINI, P. R.; FERREIRA, L. P.; VIEIRA, L. G. E.; PEREIRA, L. F. P. Açúcares solúveis, sacarose sintase e sacarose fosfato sintase durante o desenvolvimento do fruto de café, sob diferentes condições de luz e carga. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 4., 2005, Londrina. Anais... Brasília, DF: Embrapa Café, 2005.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Café. |
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14. | | BOSCO, D. D.; FERNANDO, J. A.; MAZZAFERA, P.; MAIA, J. D. G.; RITSCHEL, P. S.; QUECINI, V. Distinct transcriptional networks control environment sensing and development in wild and cultivated grapevine genotypes. In: Congresso Brasileiro de Genética, 61., 2015, Águas Lindas de Lindóia, SP. Águas Lindas de Lindóia, SP: SBG, 2015.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Uva e Vinho. |
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15. | | FELTRIM, D.; GUPTA, B.; GUNDIMEDA, S.; KIYOTA, E.; DOMINGUES JÚNIOR, A. P.; CINTRA, L. C.; MAZZAFERA, P. Exposure of Eucalyptus to varied temperature and CO2 has a profound effect on the physiology and expression of genes related to cell wall formation and remodeling. Tree Genetics & Genomes v. 18, n. 1, 3, Feb. 2022.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Agricultura Digital. |
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16. | | GEROMEL, C.; FERREIRA, L. P.; GUERREIRO, S. M. C.; CAVALARI, A. A.; POT, D.; PEREIRA, L. F. P.; LEROY, T.; VIEIRA, L. G. E.; MAZZAFERA, P.; MARRACCINI, P. Biochemical and genomic analysis of sucrose metabolism during coffee (Coffea arabica) fruit development. Journal of Experimental Botany, v. 57, n. 12, p. 3243-3258, 2006.Tipo: Artigo em Periódico Indexado |
Biblioteca(s): Embrapa Café. |
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17. | | SILVA, E. A. da; MAZZAFERA, P.; BRUNINI, O.; SAKAU, E.; ARRUDA, F. B.; MATTOSO, L. H. C.; CARVALHO, C. R. L. PIRES, R. C. M. The influence of water management and environmental conditions on the chemical composition and beverage quality of coffe beans. Brazilian Journal of Plant Physiology, Piracicaba, v. 17, n. 2, p. 229-238, June 2005.Biblioteca(s): Embrapa Instrumentação. |
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18. | | GEROMEL, C.; PEREIRA, L. P.; CAVALARI, A. A.; PEREIRA, L. F. P.; VIEIRA, L. G. E.; LEROY, T.; MAZZAFERA, P.; MARRACCINI, P. Metabolismo de açúcares durante o desenvolvimento de frutos de café. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 4., 2005, Londrina. Anais... Brasília, DF: Embrapa Café, 2005.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Café. |
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19. | | GEROMEL, C.; FERREIRA, L. P.; BOTTCHER, A.; POT, D.; PEREIRA, L. F. P.; LEROY, T.; VIEIRA, L. G. E.; MAZZAFERA, P.; MARRACCINI, P. Sucrose metabolism during fruit development in coffea racemosa. Annals of Applied Biology, v. 152, n. 2, p.179-187, 2008.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
Biblioteca(s): Embrapa Café. |
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20. | | GEROMEL, C.; FERREIRA, L. P.; CAVALARI, A. A.; PEREIRA, L. F. P.; VIEIRA, L. G. E.; LEROY, T.; MAZZAFERA, P.; MARRACCINI, P. Sugar metabolism during coffee fruit development. In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 20., 2004, Bangalore, India. Anais... Montpellier, France: Association for Science and Information on Coffee, 2005. 1 CD-ROM.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Café. |
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Registros recuperados : 29 | |
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