Data/hora: 05/06/2024 06:44:25

Biblioteca(s): | Embrapa Agricultura Digital; Embrapa Recursos Genéticos e Biotecnologia.
Data corrente: | 06/08/2008
Data da última atualização: | 24/10/2022
Autoria: | TOGAWA, R. C.; RIBEIRO, C.; MAZONI, I.; PELLIGRINELLI, T.; JARDINE, J. G.; NESHICH, G.
Afiliação: | Roberto Coiti Togawa, Embrapa Recursos Genéticos e Biotecnologia; Cristina Ribeiro, UFMG; Ivan Mazoni, Embrapa Informação Tecnógica; Thais Vital Pelligrinelli, Embrapa Informação Tecnógica; José Gilberto Jardine, Embrapa Informação Tecnógica; Goran Neshich, Embrapa Informação Tecnógica.
Título: | The table of interface forming residues as the specificity indicator for serine proteases bound to different inhibitors.
Ano de publicação: | 2008
Fonte/Imprenta: | In: INTERNATIONAL CONFERENCE ON BIOINFORMATICS & COMPUTATIONAL BIOLOGY, 2008, Georgia. Las Vegas Nevada: CSREA, 2008, p. 811-817.
Idioma: | Inglês
Notas: | BIOCOMP 2008. WORLDCOMP'08
Conteúdo: | We propose a novel method for defining the exclusive and exhaustive table of serine proteases specificity determining interface forming residues (IFR). The IFR are obtained by "hard body docking" among 73 structurally aligned, sequence wise non redundant, serine protease structures, with 3 inhibitors: ecotine, ovomucoid third domain inhibitor and basic pancreatic trypsin inhibitors. In silico constructed complexes offered a condition for determining which residues are participating, from both enzyme and inhibitor side, in the ensemble of amino acids that upon biding loose contact with a solvent. Our focus is on offering a thorough study on how the specificity is achieved among serine proteases even though they have very little difference in their tertiary structure (specifically if the position of catalytic triad residues is considered). Presented table of serine protease specificity based on IFR position occupation show clear variations among sub families such as: trypsines, chymotrypsines, elastases and thrombines.
Palavras-Chave: | Aminoácidos; Enzyme specificity; Especificidade enzimática; Hard body docking; Interface formando resíduos; Interface forming residues; Proteases de serina.
Thesagro: | Enzima.
Thesaurus Nal: | Enzymes; Serine proteinases.
Categoria do assunto: | --
Registro original: | Embrapa Recursos Genéticos e Biotecnologia (CENARGEN)

Exemplares:

CNPTIA - (12116-1) - UPC - AA - PP -  -  - 2008.00148 - DES
CNPTIA - (12116-2) - UPC - AA - DD -  -  -  - DIS
CENARGEN - (31661-1) - UPC - AA - PP - SP 19743 -  - SP 19743 - DIS