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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
23/05/2018 |
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Data da última atualização: |
23/05/2018 |
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Autoria: |
GOBBI, D. D. S.; SPINDOLA, M. G.; MORENO, L. Z.; MATAJIRA, C. E. C.; OLIVEIRA, M. G. X.; PAIXÃO, R.; FERREIRA, T. S. P.; MORENO, A. M. |
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Afiliação: |
Débora D. S. Gobbi, LSS/LEMRAL /FMVZ/USP; Maria G. Spindola, LSS/LEMRAL /FMVZ/USP; Luisa Z. Moreno, LSS/LEMRAL /FMVZ/USP; Carlos E. C. Matajira, LSS/LEMRAL /FMVZ/USP; Maria G. X. Oliveira, LSS/LEMRAL /FMVZ/USP; Renata Paixão, LSS/LEMRAL /FMVZ/USP; Thais S. P. Ferreira, LSS/LEMRAL /FMVZ/USP; Andrea M. Moreno, LSS/LEMRAL /FMVZ/USP. |
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Título: |
Isolation and molecular characterization of Arcobacter butzleri and Arcobacter cryaerophilus from the pork production chain in Brazil. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 3, p. 393-399, março 2018 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Isolamento e caracterização molecular de Arcobacter butzleri e Arcobacter cryaerophilus de linhas de abate suíno e do comércio de carne no Brasil. |
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Conteúdo: |
Arcobacter is an emerging zoonotic pathogen, and the major transmission routes to humans are the handling or consumption of contaminated raw/undercooked food products of animal origin, water and seafood. The isolation and identification of Arcobacter species are not routine in clinical laboratories; therefore, its true incidence in human infections may be underestimated. The present study aimed to isolate and characterize Arcobacter from carcasses and fecal samples collected at swine slaughterhouses and from meat markets in São Paulo State, Brazil. The isolates were identified using multiplex-PCR to differentiate the species and analyzed by single-enzyme amplified fragment length polymorphism (SE-AFLP). Arcobacter spp. were isolated from 73.0% of swine carcasses, 4% of fecal samples and 10% of pork samples. A. butzleri was the most prevalent species identified, followed by A. cryaerophilus. Interestingly, the carcasses presented higher frequency of A. butzleri isolation, whereas only A. cryaerophilus was isolated from fecal samples. SE-AFLP enabled the characterization of A. butzleri and A. cryaerophilus into 51 and 63 profiles, respectively. The great genetic heterogeneity observed for both species corroborates previous reports. This study confirms the necessity for a standard isolation protocol and the improvement of molecular tools to further elucidate Arcobacter epidemiology. |
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Palavras-Chave: |
Bacterioses; Carne suína. |
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Thesagro: |
Abatedouro; Bacteriose; Suíno. |
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Thesaurus Nal: |
Amplified fragment length polymorphism; Arcobacter butzleri; Arcobacter cryaerophilus; Slaughterhouses; Swine. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/177523/1/Isolation-and-molecular-charcaterization-of-Arcobacter-butzleri.pdf
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Marc: |
LEADER 02607naa a2200337 a 4500
001 2091855
005 2018-05-23
008 2018 bl uuuu u00u1 u #d
100 1 $aGOBBI, D. D. S.
245 $aIsolation and molecular characterization of Arcobacter butzleri and Arcobacter cryaerophilus from the pork production chain in Brazil.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Isolamento e caracterização molecular de Arcobacter butzleri e Arcobacter cryaerophilus de linhas de abate suíno e do comércio de carne no Brasil.
520 $aArcobacter is an emerging zoonotic pathogen, and the major transmission routes to humans are the handling or consumption of contaminated raw/undercooked food products of animal origin, water and seafood. The isolation and identification of Arcobacter species are not routine in clinical laboratories; therefore, its true incidence in human infections may be underestimated. The present study aimed to isolate and characterize Arcobacter from carcasses and fecal samples collected at swine slaughterhouses and from meat markets in São Paulo State, Brazil. The isolates were identified using multiplex-PCR to differentiate the species and analyzed by single-enzyme amplified fragment length polymorphism (SE-AFLP). Arcobacter spp. were isolated from 73.0% of swine carcasses, 4% of fecal samples and 10% of pork samples. A. butzleri was the most prevalent species identified, followed by A. cryaerophilus. Interestingly, the carcasses presented higher frequency of A. butzleri isolation, whereas only A. cryaerophilus was isolated from fecal samples. SE-AFLP enabled the characterization of A. butzleri and A. cryaerophilus into 51 and 63 profiles, respectively. The great genetic heterogeneity observed for both species corroborates previous reports. This study confirms the necessity for a standard isolation protocol and the improvement of molecular tools to further elucidate Arcobacter epidemiology.
650 $aAmplified fragment length polymorphism
650 $aArcobacter butzleri
650 $aArcobacter cryaerophilus
650 $aSlaughterhouses
650 $aSwine
650 $aAbatedouro
650 $aBacteriose
650 $aSuíno
653 $aBacterioses
653 $aCarne suína
700 1 $aSPINDOLA, M. G.
700 1 $aMORENO, L. Z.
700 1 $aMATAJIRA, C. E. C.
700 1 $aOLIVEIRA, M. G. X.
700 1 $aPAIXÃO, R.
700 1 $aFERREIRA, T. S. P.
700 1 $aMORENO, A. M.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 3, p. 393-399, março 2018
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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