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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
19/09/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
MARTINS, G. R.; MARINHO, R. C.; BEZERRA-JUNIOR, R. Q.; CÂMARA, L. M. C.; ALBUQUERQUE-PINTO, L. C.; TEIXEIRA, M. F. S. |
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Afiliação: |
Gabrielle R. Martins, Laboratório de Virologia/Núcleo de Pesquisa em Sanidade Animal/Programa de Pós-Graduação em Ciências Veterinárias/Universidade Estadual do Ceará; Rebeca C. Marinho, Laboratório de Virologia/Núcleo de Pesquisa em Sanidade Animal/Programa de Pós-Graduação em Ciências Veterinárias/Universidade Estadual do Ceará; Rosivaldo Q. Bezerra-Junior, Laboratório de Imunologia/Programa de Pós-Graduação em Microbiologia Médica/Universidade Federal do Ceará; Lilia M. C. Câmara, Laboratório de Virologia/Núcleo de Pesquisa em Sanidade Animal/Programa de Pós-Graduação em Ciências Veterinárias/Universidade Estadual do Ceará; Luiz C. Albuquerque-Pinto, Laboratório de Imunologia/Programa de Pós-Graduação em Microbiologia Médica/Universidade Federal do Ceará; Maria F. S. Teixeira, Laboratório de Virologia/Núcleo de Pesquisa em Sanidade Animal/Programa de Pós-Graduação em Ciências Veterinárias/Universidade Estadual do Ceará. |
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Título: |
Isolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 6, p. 643-649, jun. 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Isolamento, cultura e caracterização de células tronco mesenquimais multipotentes provenientes do sangue do cordão umbilical caprino. |
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Conteúdo: |
Mesenchymal stem cells (MSC) reside in small numbers in many adult tissues and organs, and play an active role in the homeostasis of these sites. Goat derived multipotent MSC have been established from bone marrow, adipose tissues and amniotic fluid. Umbilical cord blood (UCB) is considered an important source of these cells. However, the MSC isolation from the goat UCB has not been demonstrated. Therefore, the aim of the present study was to isolate, culture and characterize goat umbilical cord blood derived mesenchymal stem cells. MSC were isolated from UCB by Ficoll-Paque density centrifugation and cultured in DMEM supplemented with 10% or 20% FBS. FACS analysis was performed and induction lineage differentiation was made to characterize these cells. They exhibited two different populations in flow cytometry, and revealed the positive expression of CD90, CD44 and CD105, but negative staining for CD34 in larger cells, and positive stained for CD90 and CD105, but negative for CD44 and CD34 in the smaller cells. MSC from goat UCB showed capability to differentiate into chondrocytes and osteoblasts when incubated with specific differentiation medium. Present study established that goat mesenchymal stem cells can be derived successfully from umbilical cord blood. |
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Palavras-Chave: |
Células tronco; Citometria de fluxo; Diferenciação em linhagenm; Lineage differentiation; MSC; Stem cell. |
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Thesaurus Nal: |
Flow cytometry; Goats. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163981/1/Isolation-culture-and-characterization-1.pdf
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Marc: |
LEADER 02331naa a2200289 a 4500
001 2075898
005 2017-12-29
008 2017 bl uuuu u00u1 u #d
100 1 $aMARTINS, G. R.
245 $aIsolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood.$h[electronic resource]
260 $c2017
500 $aTítulo em português: Isolamento, cultura e caracterização de células tronco mesenquimais multipotentes provenientes do sangue do cordão umbilical caprino.
520 $aMesenchymal stem cells (MSC) reside in small numbers in many adult tissues and organs, and play an active role in the homeostasis of these sites. Goat derived multipotent MSC have been established from bone marrow, adipose tissues and amniotic fluid. Umbilical cord blood (UCB) is considered an important source of these cells. However, the MSC isolation from the goat UCB has not been demonstrated. Therefore, the aim of the present study was to isolate, culture and characterize goat umbilical cord blood derived mesenchymal stem cells. MSC were isolated from UCB by Ficoll-Paque density centrifugation and cultured in DMEM supplemented with 10% or 20% FBS. FACS analysis was performed and induction lineage differentiation was made to characterize these cells. They exhibited two different populations in flow cytometry, and revealed the positive expression of CD90, CD44 and CD105, but negative staining for CD34 in larger cells, and positive stained for CD90 and CD105, but negative for CD44 and CD34 in the smaller cells. MSC from goat UCB showed capability to differentiate into chondrocytes and osteoblasts when incubated with specific differentiation medium. Present study established that goat mesenchymal stem cells can be derived successfully from umbilical cord blood.
650 $aFlow cytometry
650 $aGoats
653 $aCélulas tronco
653 $aCitometria de fluxo
653 $aDiferenciação em linhagenm
653 $aLineage differentiation
653 $aMSC
653 $aStem cell
700 1 $aMARINHO, R. C.
700 1 $aBEZERRA-JUNIOR, R. Q.
700 1 $aCÂMARA, L. M. C.
700 1 $aALBUQUERQUE-PINTO, L. C.
700 1 $aTEIXEIRA, M. F. S.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 6, p. 643-649, jun. 2017.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
12/12/2018 |
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Data da última atualização: |
12/12/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
KLUSKA, S.; OLIVIERI, B. F.; BONAMY, M.; CHIAIA, H. L. J.; FEITOSA, F. L. B.; BERTON, M. P.; PERIPOLLI, E.; LEMOS, M. V. A.; TONUSSI, R. L.; LOBO, R. B.; MAGNABOSCO, C. de U.; CI CROCE, F.; OSTERSTOCK, J.; PEREIRA, A. S. C.; MUNARI, D. P.; BEZERRA, L. A.; LOPES, F. B.; BALDI, F. |
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Afiliação: |
SABRINA KLUSKA; BIANCA FERREIRA OLIVIERI; MARTIN BONAMY; HERMENEGILDO LUCAS JUSTINO; FABIELI LOISE BRAGA FEITOSA; MARIANA PIATTO BERTON; ELISA PERIPOLLI; MARCOS VINICIUS ANTUNES LEMOS; RAFAEL LARA TONUSSI; RAYSILDO BARBOSA LOBO; CLAUDIO DE ULHOA MAGNABOSCO, CPAC; FERNANDO DI CROCE; JASON OSTERSTOCK; ANGELICA SIMONE CRAVO PEREIRA; DANISIO PRADO MUNARI; LUIZ ANTONIO BEZERRA; FERNANDO BRITO LOPES; FERNANDO BALDI. |
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Título: |
Estimates of genetic parameters for growth, reproductive, and carcass traits in Nelore cattle using the single step genomic BLUP procedure. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Livestock Science, v. 216, p. 203-209, October 2018. |
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DOI: |
https://doi.org/10.1016/j.livsci.2018.08.015 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract This study was carried out to estimate the heritability and genetic correlations between adjusted weights at 210 (W210) and 450 (W450) days of age, scrotal circumference adjusted at 365 (SC365) and 450 (SC450) days of age, age at first calving (AFC), probability of precocious calving (PPC), stayability (STAY), ribeye area (REA), subcutaneous backfat thickness (BF), and rump fat (RF) traits. The covariance components and genetic parameters for all traits but PPC and STAY were estimated considering a linear animal model, for the remaining traits a threshold animal model was used. The single-step genomic BLUP (ssGBLUP) procedure was used for all traits, and the correlation estimates were obtained by two-traits analysis. The heritability estimated by single-trait analysis were 0.21 ± 0.01, 0.43 ± 0.01, 0.47 ± 0.02, 0.52 ± 0.02, 0.11 ± 0.01, 0.12 (0.07?0.16), 0.37 (0.26?0.38), 0.33 ± 0.02, 0.17 ± 0.01 and 0.33 ± 0.02 for W210, W450, SC365, SC450, AFC, PPC, STAY, REA, BF and RF, respectively. Growth traits (W210 and W450) showed high (0.95) genetic correlation with one another, moderate (0.30 to 0.66) with REA and scrotal circumferences, and low (−0.07 to 0.20) with female reproductive traits (AFC, PPC, and STAY) and fat carcass indicator traits (BF and RF). Female reproductive traits displayed moderate (−0.29 to 0.52) genetic correlations with male reproductive traits, and varied from low to moderate (−0.02 to 0.35) with carcass traits. Additionally, carcass traits exhibited low to moderate (−0.02 to 0.48) genetic correlations with all traits, except for the genetic correlation between BF and RF (0.73). Hence, higher genetic gains by direct selection would be obtained for W450, SC365, SC450, STAY, REA, and RF. Selection to increase growth traits might increase carcass yield and scrotal circumference, and it would not affect carcass fat content, precocity, and cow longevity indicator traits. The results of this study encourage the use of PPC in Nelore cattle since the selection for such trait would improve the productivity and longevity of the cows. The inclusion of carcass quality indicator traits such as BF or RF in selection indices would increase the genetic gain for carcass grade, but it will require further studies to estimate the economic value of these traits. MenosAbstract This study was carried out to estimate the heritability and genetic correlations between adjusted weights at 210 (W210) and 450 (W450) days of age, scrotal circumference adjusted at 365 (SC365) and 450 (SC450) days of age, age at first calving (AFC), probability of precocious calving (PPC), stayability (STAY), ribeye area (REA), subcutaneous backfat thickness (BF), and rump fat (RF) traits. The covariance components and genetic parameters for all traits but PPC and STAY were estimated considering a linear animal model, for the remaining traits a threshold animal model was used. The single-step genomic BLUP (ssGBLUP) procedure was used for all traits, and the correlation estimates were obtained by two-traits analysis. The heritability estimated by single-trait analysis were 0.21 ± 0.01, 0.43 ± 0.01, 0.47 ± 0.02, 0.52 ± 0.02, 0.11 ± 0.01, 0.12 (0.07?0.16), 0.37 (0.26?0.38), 0.33 ± 0.02, 0.17 ± 0.01 and 0.33 ± 0.02 for W210, W450, SC365, SC450, AFC, PPC, STAY, REA, BF and RF, respectively. Growth traits (W210 and W450) showed high (0.95) genetic correlation with one another, moderate (0.30 to 0.66) with REA and scrotal circumferences, and low (−0.07 to 0.20) with female reproductive traits (AFC, PPC, and STAY) and fat carcass indicator traits (BF and RF). Female reproductive traits displayed moderate (−0.29 to 0.52) genetic correlations with male reproductive tra... Mostrar Tudo |
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Thesagro: |
Análise Biológica; Bos Indicus; Gado de Corte; Genética Animal; Parâmetro Genético; Seleção Genética; Variação Genética. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 03701naa a2200421 a 4500
001 2101352
005 2018-12-12
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.livsci.2018.08.015$2DOI
100 1 $aKLUSKA, S.
245 $aEstimates of genetic parameters for growth, reproductive, and carcass traits in Nelore cattle using the single step genomic BLUP procedure.$h[electronic resource]
260 $c2018
520 $aAbstract This study was carried out to estimate the heritability and genetic correlations between adjusted weights at 210 (W210) and 450 (W450) days of age, scrotal circumference adjusted at 365 (SC365) and 450 (SC450) days of age, age at first calving (AFC), probability of precocious calving (PPC), stayability (STAY), ribeye area (REA), subcutaneous backfat thickness (BF), and rump fat (RF) traits. The covariance components and genetic parameters for all traits but PPC and STAY were estimated considering a linear animal model, for the remaining traits a threshold animal model was used. The single-step genomic BLUP (ssGBLUP) procedure was used for all traits, and the correlation estimates were obtained by two-traits analysis. The heritability estimated by single-trait analysis were 0.21 ± 0.01, 0.43 ± 0.01, 0.47 ± 0.02, 0.52 ± 0.02, 0.11 ± 0.01, 0.12 (0.07?0.16), 0.37 (0.26?0.38), 0.33 ± 0.02, 0.17 ± 0.01 and 0.33 ± 0.02 for W210, W450, SC365, SC450, AFC, PPC, STAY, REA, BF and RF, respectively. Growth traits (W210 and W450) showed high (0.95) genetic correlation with one another, moderate (0.30 to 0.66) with REA and scrotal circumferences, and low (−0.07 to 0.20) with female reproductive traits (AFC, PPC, and STAY) and fat carcass indicator traits (BF and RF). Female reproductive traits displayed moderate (−0.29 to 0.52) genetic correlations with male reproductive traits, and varied from low to moderate (−0.02 to 0.35) with carcass traits. Additionally, carcass traits exhibited low to moderate (−0.02 to 0.48) genetic correlations with all traits, except for the genetic correlation between BF and RF (0.73). Hence, higher genetic gains by direct selection would be obtained for W450, SC365, SC450, STAY, REA, and RF. Selection to increase growth traits might increase carcass yield and scrotal circumference, and it would not affect carcass fat content, precocity, and cow longevity indicator traits. The results of this study encourage the use of PPC in Nelore cattle since the selection for such trait would improve the productivity and longevity of the cows. The inclusion of carcass quality indicator traits such as BF or RF in selection indices would increase the genetic gain for carcass grade, but it will require further studies to estimate the economic value of these traits.
650 $aAnálise Biológica
650 $aBos Indicus
650 $aGado de Corte
650 $aGenética Animal
650 $aParâmetro Genético
650 $aSeleção Genética
650 $aVariação Genética
700 1 $aOLIVIERI, B. F.
700 1 $aBONAMY, M.
700 1 $aCHIAIA, H. L. J.
700 1 $aFEITOSA, F. L. B.
700 1 $aBERTON, M. P.
700 1 $aPERIPOLLI, E.
700 1 $aLEMOS, M. V. A.
700 1 $aTONUSSI, R. L.
700 1 $aLOBO, R. B.
700 1 $aMAGNABOSCO, C. de U.
700 1 $aCI CROCE, F.
700 1 $aOSTERSTOCK, J.
700 1 $aPEREIRA, A. S. C.
700 1 $aMUNARI, D. P.
700 1 $aBEZERRA, L. A.
700 1 $aLOPES, F. B.
700 1 $aBALDI, F.
773 $tLivestock Science$gv. 216, p. 203-209, October 2018.
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