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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
07/05/2013 |
Data da última atualização: |
17/01/2024 |
Autoria: |
MARTINS, F. S.; SARAIVA, M. V. A.; CELESTINO, J. J. H.; BRUNO, J. B.; ALMEIDA, A. P.; CUNHA, R. M. S.; SILVA, J. R. V.; CAMPELO, C. C.; LUCCI, C. M.; MATOS, M. H. T.; FIGUEIREDO, J. R. |
Título: |
Expression of protein and mRNA encoding Insulin Growth Factor-I (IGF-I) in goat ovarian follicles and the influence of IGF-I on in vitro development and survival of caprine preantral follicles. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Animal Reproduction, v. 7, n. 4, p. 349-361, Oct./Dec. 2010. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The aim of this study was to investigate the protein and mRNA expression levels of IGF-I and the effects of IGF-I on preantral follicle survival and development, using an in vitro goat ovarian cortical culture system. The ovaries were used for immunohistochemical localization of IGF-I protein or used to demonstrate mRNA expression of IGF-I. For the latter goal, preantral and antral follicles, cumulus-oocyte complex, mural granulosa and theca cells were collected to study mRNA expression. For in vitro studies, ovarian cortex were cultured for 1 and 7 days with MEM supplemented IGF-I (0, 1, 10, 50, 100 or 200 ng/ml). Immunohistochemical results showed strong reactions for IGF-I in oocytes and granulosa cells of all follicular stages, except in granulosa cells of primordial and primary follicles. mRNA expression analysis demonstrated a discrete increase in the production of IGF-I during the transition from primordial to the primary and secondary follicle stages. After 7 days of culture, addition of 50 ng/ml of IGF-I to the medium showed the greatest percentage of normal follicles when compared with other concentrations. Furthermore, the highest percentage of primary follicles was observed after 7 days of culture in MEM+plus 10 and 50 ng/ml of IGF-I. Culture of ovarian tissue for 7 days in MEM+plus 50 ng/ml of IGF-I promoted the greatest increase (P < 0.05) in follicular diameter of all of the concentrations tested. In conclusion, IGF-I protein and mRNA were expressed in all follicular categories of goat. Furthermore, IGF-I maintained preantral follicle survival, promoted primordial follicle activation and stimulated the transition from intermediate to primary follicles. MenosAbstract: The aim of this study was to investigate the protein and mRNA expression levels of IGF-I and the effects of IGF-I on preantral follicle survival and development, using an in vitro goat ovarian cortical culture system. The ovaries were used for immunohistochemical localization of IGF-I protein or used to demonstrate mRNA expression of IGF-I. For the latter goal, preantral and antral follicles, cumulus-oocyte complex, mural granulosa and theca cells were collected to study mRNA expression. For in vitro studies, ovarian cortex were cultured for 1 and 7 days with MEM supplemented IGF-I (0, 1, 10, 50, 100 or 200 ng/ml). Immunohistochemical results showed strong reactions for IGF-I in oocytes and granulosa cells of all follicular stages, except in granulosa cells of primordial and primary follicles. mRNA expression analysis demonstrated a discrete increase in the production of IGF-I during the transition from primordial to the primary and secondary follicle stages. After 7 days of culture, addition of 50 ng/ml of IGF-I to the medium showed the greatest percentage of normal follicles when compared with other concentrations. Furthermore, the highest percentage of primary follicles was observed after 7 days of culture in MEM+plus 10 and 50 ng/ml of IGF-I. Culture of ovarian tissue for 7 days in MEM+plus 50 ng/ml of IGF-I promoted the greatest increase (P < 0.05) in follicular diameter of all of the concentrations tested. In conclusion, IGF-I protein and mRNA were expressed ... Mostrar Tudo |
Palavras-Chave: |
Folículo pré-antral; IGF-I. |
Thesagro: |
Cabra; Caprino; Endocrinologia; Fator de crescimento; Hormônio; Insulina; Ovário; Reprodução animal. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02813naa a2200361 a 4500 001 1957241 005 2024-01-17 008 2010 bl uuuu u00u1 u #d 100 1 $aMARTINS, F. S. 245 $aExpression of protein and mRNA encoding Insulin Growth Factor-I (IGF-I) in goat ovarian follicles and the influence of IGF-I on in vitro development and survival of caprine preantral follicles.$h[electronic resource] 260 $c2010 520 $aAbstract: The aim of this study was to investigate the protein and mRNA expression levels of IGF-I and the effects of IGF-I on preantral follicle survival and development, using an in vitro goat ovarian cortical culture system. The ovaries were used for immunohistochemical localization of IGF-I protein or used to demonstrate mRNA expression of IGF-I. For the latter goal, preantral and antral follicles, cumulus-oocyte complex, mural granulosa and theca cells were collected to study mRNA expression. For in vitro studies, ovarian cortex were cultured for 1 and 7 days with MEM supplemented IGF-I (0, 1, 10, 50, 100 or 200 ng/ml). Immunohistochemical results showed strong reactions for IGF-I in oocytes and granulosa cells of all follicular stages, except in granulosa cells of primordial and primary follicles. mRNA expression analysis demonstrated a discrete increase in the production of IGF-I during the transition from primordial to the primary and secondary follicle stages. After 7 days of culture, addition of 50 ng/ml of IGF-I to the medium showed the greatest percentage of normal follicles when compared with other concentrations. Furthermore, the highest percentage of primary follicles was observed after 7 days of culture in MEM+plus 10 and 50 ng/ml of IGF-I. Culture of ovarian tissue for 7 days in MEM+plus 50 ng/ml of IGF-I promoted the greatest increase (P < 0.05) in follicular diameter of all of the concentrations tested. In conclusion, IGF-I protein and mRNA were expressed in all follicular categories of goat. Furthermore, IGF-I maintained preantral follicle survival, promoted primordial follicle activation and stimulated the transition from intermediate to primary follicles. 650 $aCabra 650 $aCaprino 650 $aEndocrinologia 650 $aFator de crescimento 650 $aHormônio 650 $aInsulina 650 $aOvário 650 $aReprodução animal 653 $aFolículo pré-antral 653 $aIGF-I 700 1 $aSARAIVA, M. V. A. 700 1 $aCELESTINO, J. J. H. 700 1 $aBRUNO, J. B. 700 1 $aALMEIDA, A. P. 700 1 $aCUNHA, R. M. S. 700 1 $aSILVA, J. R. V. 700 1 $aCAMPELO, C. C. 700 1 $aLUCCI, C. M. 700 1 $aMATOS, M. H. T. 700 1 $aFIGUEIREDO, J. R. 773 $tAnimal Reproduction$gv. 7, n. 4, p. 349-361, Oct./Dec. 2010.
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7. | | REIS, K. B.; CORTÊS, M. V. de C. B.; MARTINS, F. S.; FILIPPI, M. C. C. de; PAULA, J. R. de; CONCEIÇÃO, E. C. da. Characterization of rue extract and its potential for controlling rice blast. Pesquisa Agropecuária Brasileira, v. 50, n. 12, p. 1121-1130, dez. 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Unidades Centrais. |
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9. | | FRADE, M. T. S.; FIRMINO, M. de O.; MAIA, L. A.; SILVEIRA, A. M.; NASCIMENTO, M. J. R. do; MARTINS, F. S. de M.; SOUZA, A. P. de; DANTAS, A. F. M. Características epidemiológicas, clínico-patológicas e morfotintoriais de quatorze casos de nocardiose em cães. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 1, p. 99-106, janeiro 2018. Título e inglês: Epidemiological, clinical, pathological and morphotinctorial features of fourteen cases of nocardiosis in dogs.Biblioteca(s): Embrapa Unidades Centrais. |
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Registros recuperados : 9 | |
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