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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
21/11/2006 |
Data da última atualização: |
22/06/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
AGUIAR, R. W. S.; MARTINS, E. S.; VALICENTE, F. H.; CARNEIRO, N. P.; BATISTA, A. C.; MELATTI, V. M.; MONNERAT, R. G.; RIBEIRO, B. M. |
Título: |
A recombinant truncated cry1Ca protein is toxic to Lepidopteran insects and forms large cuboidal crystals in insect cells. |
Ano de publicação: |
2006 |
Fonte/Imprenta: |
Current Microbiology, v. 53, n. 4, p. 287-292, oct. 2006. |
Idioma: |
Inglês |
Conteúdo: |
A truncated version of the cry1Ca gene from Bacillus thuringiensis was introduced into the genome of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) under the control of two promoters. A recombinant virus (vSyncry1c) was isolated and used to infect insect cells in culture and insect larvae. Structural and ultrastructural analysis of insects infected with vSyncry1C showed the formation of large cuboidal crystals inside the cytoplasm of insect cells in culture and in insect cadavers late in infection. Infected insect cell extracts were analyzed by SDS-PAGE and Western blot and showed the presence of a 65-kDa polypeptide probably corresponding to the protease processed form of the toxin. Bioassays using purified recombinant toxin crystals showed a CL50 of 19.49 ng/ml for 2nd instar A. gemmatalis larvae and 114.1 ng/ml for S. frugiperda. |
Palavras-Chave: |
AcMNPV; Gene Cry1Ca. |
Thesagro: |
Bacillus Thuringiensis; Genoma; Inseto. |
Thesaurus Nal: |
Autographa californica multiple nucleopolyhedrovirus. |
Categoria do assunto: |
-- S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178044/1/ID-27630-1.pdf
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Marc: |
LEADER 01714naa a2200277 a 4500 001 1188097 005 2022-06-22 008 2006 bl uuuu u00u1 u #d 100 1 $aAGUIAR, R. W. S. 245 $aA recombinant truncated cry1Ca protein is toxic to Lepidopteran insects and forms large cuboidal crystals in insect cells.$h[electronic resource] 260 $c2006 520 $aA truncated version of the cry1Ca gene from Bacillus thuringiensis was introduced into the genome of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) under the control of two promoters. A recombinant virus (vSyncry1c) was isolated and used to infect insect cells in culture and insect larvae. Structural and ultrastructural analysis of insects infected with vSyncry1C showed the formation of large cuboidal crystals inside the cytoplasm of insect cells in culture and in insect cadavers late in infection. Infected insect cell extracts were analyzed by SDS-PAGE and Western blot and showed the presence of a 65-kDa polypeptide probably corresponding to the protease processed form of the toxin. Bioassays using purified recombinant toxin crystals showed a CL50 of 19.49 ng/ml for 2nd instar A. gemmatalis larvae and 114.1 ng/ml for S. frugiperda. 650 $aAutographa californica multiple nucleopolyhedrovirus 650 $aBacillus Thuringiensis 650 $aGenoma 650 $aInseto 653 $aAcMNPV 653 $aGene Cry1Ca 700 1 $aMARTINS, E. S. 700 1 $aVALICENTE, F. H. 700 1 $aCARNEIRO, N. P. 700 1 $aBATISTA, A. C. 700 1 $aMELATTI, V. M. 700 1 $aMONNERAT, R. G. 700 1 $aRIBEIRO, B. M. 773 $tCurrent Microbiology$gv. 53, n. 4, p. 287-292, oct. 2006.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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4. | | MARTINS, E. S.; PRACA, L. B.; MONNERAT, R. Characterization of strains of Bacillus thuringiensis effective against bolweevil Anthonomus grandis Boema, 1843. In: INTERNATIONAL COLLOQUIUM ON INVERTEBRATE PATHOLOGY AND MICROBIAL CONTROL, 8.; INTERNATIONAL CONFERENCE ON BACILLUS THURINGIENSIS 6.; ANNUAL MEETING OF THE SIP, 35., 2002, Foz do Iguassu. Program and abstracts. Londrina: Embrapa Soja: Embrapa Recursos Genéticos e Biotecnologia: UEL: SIP, 2002. p. 86. (Embrapa Soja. Documentos, 183; Embrapa Recursos Genéticos e Biotecnologia, 73).Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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17. | | MARTINS, E. S.; DUMAS, V. F.; BRAZ, S. V.; MONNERAT, R. G.; RIBEIRO, M. R. Análise funcional da proteína CRY1B de Bacillus thuyringiensis ativa contra o bicudo do algodoeiro (Anthonomus grandis boheman, 1843). In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. Resumo 068. p. 113.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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20. | | QUEIROZ, P. R.; MARTINS, E. S.; MONNERAT, R. G.; LIMA, L. H. C. Análise da variabilidade genética de populações das américas de Spodoptera frugiperda (J.E. SMITH, 1797) (LEPIDOPTERA: NOCTUIDAE) por meio de marcadores moleculares. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 9., 2004, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2004. p. 111.Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 185 | |
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