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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
18/09/2015 |
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Data da última atualização: |
01/03/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, C. G.; CUMPA, H. C. B.; FONSECA NETO, A. M. da; MARTINS, C. F.; BÁO, S. N. |
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Afiliação: |
HEIDI CHRISTINA BESSLER CUMPA, CPAC; ALVARO MORAES DA FONSECA NETO, CPAC; CARLOS FREDERICO MARTINS, CPAC; UNB. |
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Título: |
Effect of trichostatin a in cloned cattle embryo production by nuclear transfer with mesenchymal stem cells. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Animal Reproduction, v. 12, n. 3, p. 812, Jul./Sept. 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Acetylation of histones is a major mechanism of genome epigenetic reprogramming of the gametes in order to establish a totipotent state to the normal development (Ikeda et al., Zygote 17, 209-215, 2009). The deacetylation is catalysed by histone deacetylases (HDAC) which remove acetyl groups and causes chromatin compaction and DNA segment silencing at this location (Johnstone, Nature Reviews Drug Discovery 1, 287-299, 2002). The trichostatin A (TSA) is an HDAC inhibitor that increases the amount of acetylated histones and the demethylation of DNA (Lee et al., Journal of Reproduction and Development 57, 34-42, 2011). In this sense, the drug has been used in an attempt to increase the production efficiency of embryos by nuclear transfer (NT). The objective of this study was to test the effect of TSA in exposure times of 20 and 25 hours in the culture of bovine embryos cloned by NT with mesenchymal stem cells (MSCs) derived from adipose tissue. A biopsy of skin and adipose tissue was collected from the perineal region of a female bovine Gir, at two months of age. The cells were isolated by the explant and cultured in Dulbeccos Modified Eagle Medium added with 10% fetal bovine serum. Cumulus-oocyte complexes recovered from slaughterhouse ovaries were matured for 18 h at 38.5°C and 5% CO2. The NT was then performed with the adipose tissue and the MSCs reconstructed embryos were subjected to culture with 50 nM TSA for 20 and 25 h, for 4 h in activation medium containing 6-DMAP and further for 16 or 21 h in medium cultivation. Subsequently, the embryos continued in culture in synthetic oviductal fluid (SOF) medium without TSA and the parthenogenetic control was performed in every manipulation. Five NT procedures were performed for each treatment (20 h, 25 h and without TSA). Fusion rates, cleavage and blastocyst production were compared by Tukey test (p <0.05). The cleavage rate of parthenogenetic embryos (93.45 ± 7.97) was higher than the cleavage rate of embryos without treatment (82.45 ± 5.59) but was statistically similar to embryos treated with 20 and 25 h (87.25 ± 8.41 and 85.54 ± 3.88, respectively). Still, there was no difference in cleavage rate between treated and untreated embryos. The blastocyst production rate on the seventh day of culture was superior to the parthenogenetic control (59.24 ± 11.75) compared to treatments for 20 h (36.22 ± 16.80), 25 h (33.66 ± 12.84) and without the use of TSA (32.70 ± 9.11), which did not differ. It can be concluded that the use of TSA had no significant effect in improving the rates of cleavage and development of bovine embryos by nuclear transfer with MSCs from adipose tissue. However, additional studies to evaluate the quality and embryos of the methylation pattern should be conducted to better understand the effects of TSA in embryos cloned with this cell type. MenosAcetylation of histones is a major mechanism of genome epigenetic reprogramming of the gametes in order to establish a totipotent state to the normal development (Ikeda et al., Zygote 17, 209-215, 2009). The deacetylation is catalysed by histone deacetylases (HDAC) which remove acetyl groups and causes chromatin compaction and DNA segment silencing at this location (Johnstone, Nature Reviews Drug Discovery 1, 287-299, 2002). The trichostatin A (TSA) is an HDAC inhibitor that increases the amount of acetylated histones and the demethylation of DNA (Lee et al., Journal of Reproduction and Development 57, 34-42, 2011). In this sense, the drug has been used in an attempt to increase the production efficiency of embryos by nuclear transfer (NT). The objective of this study was to test the effect of TSA in exposure times of 20 and 25 hours in the culture of bovine embryos cloned by NT with mesenchymal stem cells (MSCs) derived from adipose tissue. A biopsy of skin and adipose tissue was collected from the perineal region of a female bovine Gir, at two months of age. The cells were isolated by the explant and cultured in Dulbeccos Modified Eagle Medium added with 10% fetal bovine serum. Cumulus-oocyte complexes recovered from slaughterhouse ovaries were matured for 18 h at 38.5°C and 5% CO2. The NT was then performed with the adipose tissue and the MSCs reconstructed embryos were subjected to culture with 50 nM TSA for 20 and 25 h, for 4 h in activation medium containing 6-DMAP and... Mostrar Tudo |
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Palavras-Chave: |
Cloning; Epigenetic. |
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Thesagro: |
Clonagem. |
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Thesaurus Nal: |
histone deacetylase. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/129960/1/34712.pdf
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Marc: |
LEADER 03483nam a2200205 a 4500
001 2024379
005 2016-03-01
008 2015 bl uuuu u00u1 u #d
100 1 $aSILVA, C. G.
245 $aEffect of trichostatin a in cloned cattle embryo production by nuclear transfer with mesenchymal stem cells.
260 $aAnimal Reproduction, v. 12, n. 3, p. 812, Jul./Sept. 2015.$c2015
520 $aAcetylation of histones is a major mechanism of genome epigenetic reprogramming of the gametes in order to establish a totipotent state to the normal development (Ikeda et al., Zygote 17, 209-215, 2009). The deacetylation is catalysed by histone deacetylases (HDAC) which remove acetyl groups and causes chromatin compaction and DNA segment silencing at this location (Johnstone, Nature Reviews Drug Discovery 1, 287-299, 2002). The trichostatin A (TSA) is an HDAC inhibitor that increases the amount of acetylated histones and the demethylation of DNA (Lee et al., Journal of Reproduction and Development 57, 34-42, 2011). In this sense, the drug has been used in an attempt to increase the production efficiency of embryos by nuclear transfer (NT). The objective of this study was to test the effect of TSA in exposure times of 20 and 25 hours in the culture of bovine embryos cloned by NT with mesenchymal stem cells (MSCs) derived from adipose tissue. A biopsy of skin and adipose tissue was collected from the perineal region of a female bovine Gir, at two months of age. The cells were isolated by the explant and cultured in Dulbeccos Modified Eagle Medium added with 10% fetal bovine serum. Cumulus-oocyte complexes recovered from slaughterhouse ovaries were matured for 18 h at 38.5°C and 5% CO2. The NT was then performed with the adipose tissue and the MSCs reconstructed embryos were subjected to culture with 50 nM TSA for 20 and 25 h, for 4 h in activation medium containing 6-DMAP and further for 16 or 21 h in medium cultivation. Subsequently, the embryos continued in culture in synthetic oviductal fluid (SOF) medium without TSA and the parthenogenetic control was performed in every manipulation. Five NT procedures were performed for each treatment (20 h, 25 h and without TSA). Fusion rates, cleavage and blastocyst production were compared by Tukey test (p <0.05). The cleavage rate of parthenogenetic embryos (93.45 ± 7.97) was higher than the cleavage rate of embryos without treatment (82.45 ± 5.59) but was statistically similar to embryos treated with 20 and 25 h (87.25 ± 8.41 and 85.54 ± 3.88, respectively). Still, there was no difference in cleavage rate between treated and untreated embryos. The blastocyst production rate on the seventh day of culture was superior to the parthenogenetic control (59.24 ± 11.75) compared to treatments for 20 h (36.22 ± 16.80), 25 h (33.66 ± 12.84) and without the use of TSA (32.70 ± 9.11), which did not differ. It can be concluded that the use of TSA had no significant effect in improving the rates of cleavage and development of bovine embryos by nuclear transfer with MSCs from adipose tissue. However, additional studies to evaluate the quality and embryos of the methylation pattern should be conducted to better understand the effects of TSA in embryos cloned with this cell type.
650 $ahistone deacetylase
650 $aClonagem
653 $aCloning
653 $aEpigenetic
700 1 $aCUMPA, H. C. B.
700 1 $aFONSECA NETO, A. M. da
700 1 $aMARTINS, C. F.
700 1 $aBÁO, S. N.
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Registro original: |
Embrapa Cerrados (CPAC) |
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| Registros recuperados : 175 | |
| 81. |  | SIQUEIRA, K. M. M. de; MARTINS, C. F.; SCHLINDWEIN, C.; KIILL, L. H. P.; MONTEIRO, S. P. Estratégia reprodutiva do maracujá amarelo (Passiflora edulis Sims F. flavicarpa Deg) em área irrigada do Vale do Submédio São Francisco. In: REUNIÃO NORDESTINA DE BOTÂNICA, 29., 2006, Mossoró. Diversidade, conservação e uso sustentável da flora nordestina: resumos. Mossoró: UERN, 2006. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
|    |
| 82. | | DIESEL, T. O.; MARQUES, T. C.; MARTINS, C. F.; MOREIRA, P. V. S.; MOREIRA, R. C.; GAMBARINI, M. L. Meio de cultivo in vitro suplementado com L-carnitina na produção e viabilidade de embriões bovinos vitrificados. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CERRADOS. Jovens talentos 2016: resumos. Planaltina, DF: Embrapa Cerrados, 2016. 99 p. p. 43 (Embrapa Cerrados. Documentos, 334) Comissão organizadora: SOUZA, K. W. de; FALEIRO, A. S. G.; SALGUES, A. L. M.; ALONSO, A. M.; CARVALHO, A. M. de; LOBATO, B. R.; CRUZ, C. V.; SOUSA, E. dos S.; PELEGRINELLI, F.; SOARES, J. P. G.; LIMA, J. E. F. W.; ARBUES, J. F. de O.;...| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| 83. | | FEIJÓ, J. de O.; SCHMITT, E.; MARTINS, C. F.; DEL PINO, F. A. B.; RABASSA, V. R.; CORRÊA, M. N. Protocolo de indução de cetose sub-clínica em ovelhas prenhes e seu efeito sobre parâmetros metabólicos. In: ENCONTRO DE PÓS GRADUAÇÃO UFPEL, 15., 2012, Pelotas - RS. Anais... Pelotas: UFPel, 2012.| Biblioteca(s): Embrapa Rondônia. |
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| 86. | | MARTINS, C. F.; DRIESSEN, K.; COSTA, P. M.; CARVALHO-NETO, J. O.; SOUSA, R. V.; RUMPF, R.; DODE, M. N. Recovery, cryopreservation and fertilization potential of bovine spermatozoa obtained from epididymides stored at 5 .C by different periods of time Animal Reproduction Science, v. 116, n. 1-2, nov. 2009, p. 50-57| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Cerrados. |
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| 88. | | FERRA, J. de C.; CIESLAK, S.; SARTORI FILHO, R.; MCMANUS, C.; MARTINS, C. F.; SERENO, J. R. B. Weight and age at puberty and their correlations with morphometric measurements in crossbred breed suffolk ewe lambs. Revista Brasileira de Zootecnia, v. 39, n. 1, p. 134-141, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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| 89. | | ABUD, L. J.; GUIMARÃES, C. O.; OLIVEIRA NETO, J. C. de; DODE, M. N.; OLIVEIRA, C. T. S. A. M.; SCHWARZ, D. G. G.; MARTINS, C. F. Avaliação da eficiência de diferentes métodos de criopreservação de sêmen bovino. In: ENCONTRO DE JOVENS TALENTOS DA EMBRAPA CERRADOS, 4., 2009, Planaltina, DF. Resumos apresentados... Planaltina, DF: Embrapa Cerrados, 2009. p. 186-187 (Embrapa Cerrados. Documentos, 243).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| 90. | | SIQUEIRA, K. M. M. de; MARTINS, C. F.; SCHLINWEN, C.; KIILL, L. H. P.; MONTEIRO, S. P.; FEITOZA, E. de A.; LEMOS, I. B. Associação entre o comportamento de forrageamento de espécies de Xylocopa e morfologia floral de Passiflora edulis Sims Flavicarpa Deg, em área irrigada no Vale do Submédio São Francisco. In : ENCONTRO SOBRE ABELHAS, 7., 2006, Ribeirão Preto. Anais... Ribeirão Preto: USP, 2006. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 91. | | IANELLA, P.; MCMANUS, C. P.; CAETANO, A. R.; MARTINS, C. F.; SOUZA, C. J. H. de; FACÓ, O.; AZEVEDO, H. C.; CARNEIRO, P. S.; PAIVA, S. R. Avaliação dos polimorfismos do gene PRNP ligados à Scrapie clássica em núcleos de conservação de ovinos no Brasil. In: SIMPOSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA Y EL CARIBE, 7., 2009, Pucón. Proceeding... Santiago: INIA, 2009. p. 265-266. VII SIRGEALC.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 92. | | IANELLA, P.; McMANUS, C. P.; CAETANO, A. R.; MARTINS, C. F.; SOUZA, C. J. H. de; FACÓ, O.; AZEVEDO, H. C.; CARNEIRO, P. S.; PAIVA, S. R. Avaliação dos polimorfismos do gene PRNP ligados à Scrapie clássica em núcleos de conservação de ovinos no Brasil. In: SIMPOSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA Y EL CARIBE, 7., 2009, Pucón. Proceeding... Santiago: INIA, 2009. p. 265-266. VII SIRGEALC.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 93. | | IANELLA, P.; MCMANUS, C. P.; CAETANO, A. R.; MARTINS, C. F.; SOUZA, C. J. H. de; FACÓ, O.; AZEVEDO, H. C.; CARNEIRO, P. S.; PAIVA, S. R. Avaliação dos polimorfismos do gene PRNP ligados à Scrapie clássica em núcleos de conservação de ovinos no Brasil. In: SIMPOSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA Y EL CARIBE, 7., 2009, Pucón. Proceeding... Santiago: INIA, 2009. p. 265-266. VII SIRGEALC.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 94. | | SIQUEIRA, K. M. M. de; KIILL, L. H. P.; MARTINS, C. F.; SCHILINDWEIN, C.; MONTEIRO, S. P.; FEITOSA, E. de A.; LEMOS, I. B. Biologia floral do maracujá amarelo (Passiflora edulis f. flavicarpa Deg) em área irrigada no Submédio do Vale do São Francisco. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMI-ÁRIDO, 1., 2006, Petrolina. Anais... Petrolina: Embrapa Semi-Árido, 2006. p. 147-151. (Embrapa Semi-Árido. Documentos, 197).| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Semiárido. |
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| 95. | | ABUD, L. J.; ABUD, C. O. G.; COSTA, G. L.; FIORAVANTI, M. C. S.; MARTINS, C. F.; PIMENTEL, C. M. M.; SERENO, J. R. B. Correlation between age, weight and body measures at first pregnancy of nellore heifers. Archives of Veterinary Science, v. 23, n. 3, p. 80-88, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Cerrados. |
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| 96. | | MARQUES, T. C.; DIESEL, T. O.; SILVA, C. G. da; SANTOS, E. C. S.; MARTINS, C. F.; DODE, M. A. N.; GAMBARINI, M. L. Blastocoel collapse associated with melatonin in the culture media improves bovine embryo viability after vitrification. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECHNOLOGY SOCIETY, 30., 2016, Foz do Iguaçu. Proceedings... Foz do Iguaçu: SBTE, 2016. Support Biotechnologies: Cryopreservation and cryobiology, diagnosis through imaging, molecular biology and "omics" (Abstracts A325). Publicado em Animal Reproduction, v. 13, n. 3, p. 699, jul./set. 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 97. | | MARQUES, T. C.; DIESEL, T. O.; SILVA, C. G. da; SANTOS, E. C. S.; MARTINS, C. F.; DODE, M. A. N.; GAMBARINI, M. L. Blastocoel collapse associated with melatonin in the culture media improves bovine embryo viability after vitrification. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECHNOLOGY SOCIETY, 30., 2016, Foz do Iguaçu. Proceedings... Foz do Iguaçu: SBTE, 2016. Support Biotechnologies: Cryopreservation and cryobiology, diagnosis through imaging, molecular biology and "omics" (Abstracts A325). Publicado em Animal Reproduction, v. 13, n. 3, p. 699, jul./set. 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
| |
| 98. | | CUNHA, E. R. da; SILVA, C. G. da; CUNHA, A. M.; REZENDE, L. O. F.; MARTINS, G. H. L.; CAMPOS, H. F.; MARTINS, C. F. Comparação de diferentes métodos para a avaliação da morfologia, integridade de membrana plasmática e acrossoma de sêmen criopreservado bovino importado. In:REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 25., 2011, Cumbuco. O impacto das biotecnologias reprodutivas na saúde e produção animal: anais. Cumbuco: SBTE, 2011. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| 99. | | ABUD, C. O. G.; ABUD, L. J.; OLIVEIRA NETO, J. C.; DODE, M. A. N.; SERENO, J. R. B.; MARTINS, C. F. Comparação entre os sistemas automatizado e convencional de criopreservação de sêmen bovino. Ciência Animal Brasileira, Goiânia, v. 15, n. 1, p. 32-37, jan./mar. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Cerrados. |
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| 100. | | SILVA, D. de O.; MARTINS, G. R.; SILVA, C. G. M.; ANTUNES NETO, A.; MARTINS, C. F. A.; PASSOS, A. M. A. dos. Caracterização de sementes de acessos do banco ativo de germoplasma de sorgo por visão computacional. In: CONGRESSO NACIONAL DE MILHO E SORGO, 33., 2022, Sete Lagoas. Brasil: 200 anos de independência: sustentabilidade e desafios para a cadeia produtiva de grãos: resumos. Sete Lagoas: Associação Brasileira de Milho e Sorgo, 2022. Evento online.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 175 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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