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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
29/05/2023 |
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Data da última atualização: |
25/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PEREIRA, B. M.; ARRAES, F.; MARTINS, A. C. Q.; ALVES, N. S. F.; MELO, B. P.; MORGANTE, C. V.; SARAIVA, M. A. P.; SA, M. F. G. de; GUIMARAES, P. M.; BRASILEIRO, A. C. M. |
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Afiliação: |
BRUNA MEDEIROS PEREIRA, UNIVERSIDADE DE BRASÍLIA; FABRÍCIO ARRAES, UNIVERSIDADE DE BRASILIA; ANDRESSA CUNHA QUINTANA MARTINS, UNIVERSIDADE CATOLICA DE BRASILIA; NAYARA SABRINA FREITAS ALVES, UNIVERSIDADE FEDERAL DO PARANÁ; BRUNO PAES MELO, LONGPING HIGH TECH; CAROLINA VIANNA MORGANTE, CPATSA; MARIO ALFREDO PASSOS SARAIVA, INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA; MARIA FATIMA GROSSI DE SA, CENARGEN; PATRICIA MESSEMBERG GUIMARAES, CENARGEN; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN. |
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Título: |
A novel soybean hairy root system for gene functional validation. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Plos One, v. 18, n. 5, e0285504, 2023. |
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DOI: |
https://doi. org/10.1371/journal.pone.0285504 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Maria Fátima Grossi-de-Sa; Patricia Messenberg Guimaraes. |
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Conteúdo: |
Agrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suitable for high throughput in root analysis of candidate genes in soybean. MenosAgrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suita... Mostrar Tudo |
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Palavras-Chave: |
Agrobacterium rhizogenes; Transgenic soybean. |
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Thesagro: |
Glycine Max; Melhoramento Genético Vegetal; Meloidogyne spp; Soja. |
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Thesaurus Nal: |
Soybeans. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1154066/1/A-novel-soybean-hairy-root-system-for-gene-functional-validation-2023.pdf
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Marc: |
LEADER 02598naa a2200337 a 4500
001 2154066
005 2024-04-25
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi. org/10.1371/journal.pone.0285504$2DOI
100 1 $aPEREIRA, B. M.
245 $aA novel soybean hairy root system for gene functional validation.$h[electronic resource]
260 $c2023
500 $aNa publicação: Maria Fátima Grossi-de-Sa; Patricia Messenberg Guimaraes.
520 $aAgrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suitable for high throughput in root analysis of candidate genes in soybean.
650 $aSoybeans
650 $aGlycine Max
650 $aMelhoramento Genético Vegetal
650 $aMeloidogyne spp
650 $aSoja
653 $aAgrobacterium rhizogenes
653 $aTransgenic soybean
700 1 $aARRAES, F.
700 1 $aMARTINS, A. C. Q.
700 1 $aALVES, N. S. F.
700 1 $aMELO, B. P.
700 1 $aMORGANTE, C. V.
700 1 $aSARAIVA, M. A. P.
700 1 $aSA, M. F. G. de
700 1 $aGUIMARAES, P. M.
700 1 $aBRASILEIRO, A. C. M.
773 $tPlos One$gv. 18, n. 5, e0285504, 2023.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Biblioteca |
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| Registros recuperados : 68 | |
| 61. |  | BRASILEIRO, A. C. M.; MORGANTE, C. V.; BERTIOLI, S. C. de M. L.; ARAUJO, A. C. G. de; PAPPAS, G.; SILVA JUNIOR, O. B. da; MARTINS, A. C. Q.; BERTIOLI, D.; GUIMARAES, P. M. Transcriptome analysis in response to gradual water deficit in Arachis wild relatives. In: INTERNATIONAL CONFERENCE ON LEGUME GENETICS AND GENOMICS, 6., 2012, Hyderabad, India. Programe & abstract book... Hyderabad: ICRISAT, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 62. | | MARTINS, A. C. Q.; MORGANTE, C. V.; SANTOS, C. M. R.; DA SILVA, F. R.; LEAL BERTIOLI, S. C. M.; BERTIOLI, D. J.; GUIMARÃES, P. M.; BRASILEIRO, A. C. M. Transcriptome analysis of wild Arachis under drought stress. In: CONGRESSO BRASILEIRO DE GENÉTICA, 54., 2008, Salvador. Resumos... Salvador: SBG, 2008. p. 305. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 63. | | BRASILEIRO, A. C. M.; BERTIOLI, S. C. de M. L.; MORGANTE, C. V.; MARTINS, A. C. Q.; SANTOS, C. M. R.; ARAUJO, A. C. G. de; SILVA, F. R.; BERTIOLI, D. J.; GUIMARAES, P. M. Transcriptome analysis of wild Arachis under water stress. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY, 4, 2009, Mali, África. Advances in Arachis through genomics and biotechnology (AAGB-2009). Mali: International Crops Research Institute for the Semi-Arid Tropics, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 64. | | MARTINS, A. C. Q.; MORGANTE, C. V.; SILVA, A. K.; GALHARDO, I. C.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, S. C. de M. L.; BERTIOLI, D. J.; MILLER, R. N. G.; BRASILEIRO, A. C. M.; GUIMARAES, P. M. Large scale transcriptome analysis of wild peanut (Arachis stenosperma) inoculated with Passalora personata, the causal agent of late leaf spot. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. p. 76.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 65. | | MARTINS, A. C. Q.; MORGANTE, C. V.; SILVA, A. K.; GALHARDO, I. C.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, S. C. de M. L.; BERTIOLI, D. J.; MILLER, R. N. G.; BRASILEIRO, A. C. M.; GUIMARAES, P. M. Large scale transcriptome analysis of wild peanut (Arachis stenosperma) inoculated with Passalora personata, the causal agent of late leaf spot. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. p. 76.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 66. | | GUIMARAES, P. M.; BRASILEIRO, A. C. M.; MORGANTE, C. V.; MARTINS, A. C. Q.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; TOGAWA, R. C.; BERTIOLI, S. C. de M. L.; ARAUJO, A. C. G. de; MORETZSOHN, M. de C.; BERTIOLI, D. J. Global transcriptome analysis of two wild relatives of peanut under drought and fungi infection. BMC Genomics, v. 13, n. 387, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 67. | | GUIMARAES, P. M.; GUIMARAES, L. A.; MORGANTE, C. V.; SILVA JUNIOR, O. B.; ARAUJO, A. C. G.; MARTINS, A. C. Q.; SARAIVA, M. A. P.; OLIVEIRA, T. N.; TOGAWA, R. C.; LEAL-BERTIOLI, S. C. M.; BERTIOLI, D. J.; BRASILEIRO, A. C. M. Root transcriptome analysis of wild peanut reveals candidate genes for nematode resistance. Plos One, October 21, p. 1-22, 2015. (Open Access).| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 68. | | BRASILEIRO, A. C. M.; MORGANTE, C. V.; ARAUJO, A. C. G.; LEAL-BERTIOLI, S. C. M.; SILVA, A. K.; MARTINS, A. C. Q.; VINSON, C. C.; SANTOS, C. M. R.; BONFIM, O.; TOGAWA, R. C.; SARAIVA, M. A. P.; BERTIOLI, D. J.; GUIMARAES, P. M. Transcriptome profiling of wild Arachis from water-limited environments uncovers drought tolerance candidate genes. Plant Molecular Biology Reporter, v. 33, p. 1876-1892, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| Registros recuperados : 68 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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