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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
21/11/2017 |
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Data da última atualização: |
16/05/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARTINI, C. L.; LANGE, C. C.; BRITO, M. A. V. P. e; RIBEIRO, J. B.; MENDONCA, L. C.; VAZ, E. K. |
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Afiliação: |
CAROLINE LOPES MARTINI, UFRJ; CARLA CHRISTINE LANGE, CNPGL; MARIA APARECIDA V PAIVA E BRITO, CNPGL; JOAO BATISTA RIBEIRO, CNPGL; LETICIA CALDAS MENDONCA, CNPGL; ELIANA KNACKFUSS VAZ, UNIVERSIDADE DO ESTADO DE SANTA CATARINA. |
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Título: |
Characterization of penicillin and tetracycline resistance in Staphylococcus aureus isolated from bovine milk samples in Minas Gerais, Brazil. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Journal of Dairy Research, v. 84, p. 202-205, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
This Regional Research Communication describes the characterisation of ampicillin, penicillin and tetracycline resistance in Staphylococcus aureus isolated from bovine subclinical mastitis in Minas Gerais State, Brazil. Ninety S. aureus isolates from bovine mastitis exhibiting phenotypic resistance to ampicillin, penicillin and/or tetracycline were selected for this study. The minimum inhibitory concentration (MIC) of each antibiotic was determined using the E-Test® and the production of beta-lactamase was determined by cefinase disks. The resistance genes blaZ, tet(K), tet(L), tet(M), and tet(O) were investigated by PCR in all of the isolates. The MIC results classified 77, 83 and 71% of the isolates as resistant to ampicillin, penicillin and tetracycline, respectively. The MIC50 and MIC90 were, respectively, 1 and 2 µg/ml for ampicillin, 0·5 and 1 µg/ml for penicillin and 32 and 64 µg/ml for tetracycline. Eighty-six per cent of beta-lactamase producing isolates were detected. Of the 90 isolates investigated, 97% amplified blaZ, 84% amplified tet(K), 9% amplified tet(L), 2% amplified tet(M) and 1% amplified tet(O). Seventy-nine isolates (88%) showed blaZ together with at least one tet gene. S. aureus isolates showed high MIC50 and MIC90 values for the three antimicrobials. The blaZ and tet(K) genes were widespread in the herds studied, and most of the isolates harboured blaZ and tet(K) concomitantly. |
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Palavras-Chave: |
BlaZ; E-Test®; MIC; Tet genes. |
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Thesaurus Nal: |
beta-lactamase. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02149naa a2200241 a 4500
001 2080328
005 2024-05-16
008 2017 bl uuuu u00u1 u #d
100 1 $aMARTINI, C. L.
245 $aCharacterization of penicillin and tetracycline resistance in Staphylococcus aureus isolated from bovine milk samples in Minas Gerais, Brazil.$h[electronic resource]
260 $c2017
520 $aThis Regional Research Communication describes the characterisation of ampicillin, penicillin and tetracycline resistance in Staphylococcus aureus isolated from bovine subclinical mastitis in Minas Gerais State, Brazil. Ninety S. aureus isolates from bovine mastitis exhibiting phenotypic resistance to ampicillin, penicillin and/or tetracycline were selected for this study. The minimum inhibitory concentration (MIC) of each antibiotic was determined using the E-Test® and the production of beta-lactamase was determined by cefinase disks. The resistance genes blaZ, tet(K), tet(L), tet(M), and tet(O) were investigated by PCR in all of the isolates. The MIC results classified 77, 83 and 71% of the isolates as resistant to ampicillin, penicillin and tetracycline, respectively. The MIC50 and MIC90 were, respectively, 1 and 2 µg/ml for ampicillin, 0·5 and 1 µg/ml for penicillin and 32 and 64 µg/ml for tetracycline. Eighty-six per cent of beta-lactamase producing isolates were detected. Of the 90 isolates investigated, 97% amplified blaZ, 84% amplified tet(K), 9% amplified tet(L), 2% amplified tet(M) and 1% amplified tet(O). Seventy-nine isolates (88%) showed blaZ together with at least one tet gene. S. aureus isolates showed high MIC50 and MIC90 values for the three antimicrobials. The blaZ and tet(K) genes were widespread in the herds studied, and most of the isolates harboured blaZ and tet(K) concomitantly.
650 $abeta-lactamase
653 $aBlaZ
653 $aE-Test®
653 $aMIC
653 $aTet genes
700 1 $aLANGE, C. C.
700 1 $aBRITO, M. A. V. P. e
700 1 $aRIBEIRO, J. B.
700 1 $aMENDONCA, L. C.
700 1 $aVAZ, E. K.
773 $tJournal of Dairy Research$gv. 84, p. 202-205, 2017.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
19/09/2023 |
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Data da última atualização: |
19/09/2023 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
YASSITEPE, J. E. de C. T.; DANTE, R. A.; GERHARDT, I. R.; FERNANDES, F. R.; SOUZA, R. S. C. de; ARMANHI, J. S. L.; SILVA, V. C. H. da; RIBEIRO, A. P.; SILVA, M. J. da; ARRUDA, P. |
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Afiliação: |
JULIANA ERIKA DE C T YASSITEPE, CNPTIA; RICARDO AUGUSTO DANTE, CNPTIA; ISABEL RODRIGUES GERHARDT, CNPTIA; FERNANDA RAUSCH FERNANDES, CNPTIA; RAFAEL SOARES CORREA DE SOUZA, Unicamp, GCCRC; JADERSON SILVEIRA LEITE ARMANHI, Unicamp, GCCRC; VIVIANE CRISTINA HEINZEN DA SILVA, Unicamp, GCCRC; ANA PAULA RIBEIRO, Unicamp, GCCRC; MÁRCIO JOSÉ DA SILVA, Unicamp, GCCRC; PAULO ARRUDA, Unicamp, GCCRC. |
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Título: |
Genomics applied to climate change: Biotechnology for digital agriculture. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
In: MASSRUHÁ, S. M. F. S.; LEITE, M. A. de A.; OLIVEIRA, S. R. de M.; MEIRA, C. A. A.; LUCHIARI JUNIOR, A.; BOLFE, E. L. (ed.). Digital agriculture: research, development and innovation in production chains. Brasília, DF: Embrapa, 2023. cap. 11, p. 195-208. |
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ISBN: |
978-65-89957-72-0 |
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Idioma: |
Inglês |
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Conteúdo: |
Introduction. The GCCRC research pipeline: Discovery of genes and microorganisms; Proof of concept; Enhancement, large-scale testing, pre-release and release. Final considerations. |
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Palavras-Chave: |
Agricultura digital; Centro de Pesquisa em Genômica Aplicada às Mudanças Climáticas; Digital agriculture; Genômica; Mudanças climáticas. |
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Thesagro: |
Agricultura; Biotecnologia. |
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Thesaurus NAL: |
Agriculture; Biotechnology; Climate change; Genomics. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156765/1/LV-Digital-agriculture-2023-cap11.pdf
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Marc: |
LEADER 01485naa a2200373 a 4500
001 2156765
005 2023-09-19
008 2023 bl uuuu u00u1 u #d
020 $a978-65-89957-72-0
100 1 $aYASSITEPE, J. E. de C. T.
245 $aGenomics applied to climate change$bBiotechnology for digital agriculture.$h[electronic resource]
260 $c2023
520 $aIntroduction. The GCCRC research pipeline: Discovery of genes and microorganisms; Proof of concept; Enhancement, large-scale testing, pre-release and release. Final considerations.
650 $aAgriculture
650 $aBiotechnology
650 $aClimate change
650 $aGenomics
650 $aAgricultura
650 $aBiotecnologia
653 $aAgricultura digital
653 $aCentro de Pesquisa em Genômica Aplicada às Mudanças Climáticas
653 $aDigital agriculture
653 $aGenômica
653 $aMudanças climáticas
700 1 $aDANTE, R. A.
700 1 $aGERHARDT, I. R.
700 1 $aFERNANDES, F. R.
700 1 $aSOUZA, R. S. C. de
700 1 $aARMANHI, J. S. L.
700 1 $aSILVA, V. C. H. da
700 1 $aRIBEIRO, A. P.
700 1 $aSILVA, M. J. da
700 1 $aARRUDA, P.
773 $tIn: MASSRUHÁ, S. M. F. S.; LEITE, M. A. de A.; OLIVEIRA, S. R. de M.; MEIRA, C. A. A.; LUCHIARI JUNIOR, A.; BOLFE, E. L. (ed.). Digital agriculture: research, development and innovation in production chains. Brasília, DF: Embrapa, 2023. cap. 11, p. 195-208.
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